We narrowed to 3,792 results for: golden gate

1. pGG-BC-TurboID-GOI

   Plasmid

   #209390

   Purpose

   Golden Gate compatible plasmid for TurboID-based proximity labelling experiment in planta

   Depositor

   Rosa Lozano-Durán

   Article

   Tan et al J Integr Plant Biol. 2024 Jan 31. doi: 1

   Type

   Empty backbone

   Expression

   Plant

   Mutation

   TurboID gene sequence C249A

   Available Since

   Nov. 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGG-GOI-DE-TurboID

   Plasmid

   #209388

   Purpose

   Golden Gate compatible plasmid for TurboID-based proximity labelling experiment in planta

   Depositor

   Rosa Lozano-Durán

   Article

   Tan et al J Integr Plant Biol. 2024 Jan 31. doi: 1

   Type

   Empty backbone

   Expression

   Plant

   Mutation

   TurboID gene sequence C249A

   Available Since

   Nov. 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pYPQ143

   Plasmid

   #69295

   Purpose

   Golden Gate recipient and Gateway entry vector; assembly of 3 gRNAs

   Depositor

   Yiping Qi

   Article

   Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

   Type

   Empty backbone

   Use

   CRISPR

   Available Since

   Nov. 4, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. sgRNA_expression_vector

   Plasmid

   #210212

   Purpose

   An empty gRNA expression vector to clone U6 promoter driven sgRNAs with gRNA scaffold and with co-expression of DsRed. sgRNA can be cloned by Golden Gate Assembly or restriction digestion using BbsI.

   Depositor

   Albert Jeltsch

   Article

   Rajaram et al Methods Mol Biol. 2024;2842:179-192. doi

   Type

   Empty backbone

   Expression

   Bacterial and Mammalian

   Promoter

   U6

   Available Since

   Dec. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEC2483 (pSpy1C)

   Plasmid

   #220248

   Purpose

   replicative E. coli-S. pyogenes shuttle plasmid for heterologous gene expression

   Depositor

   Emmanuelle Charpentier

   Article

   Lautenschlager et al Front Bioeng Biotechnol. 2024 Jun 7;12:1

   Type

   Empty backbone

   Expression

   Bacterial

   Available Since

   Sept. 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCHW111040

   Plasmid

   #222451

   Purpose

   Level 1, Position 3 containing CBS4-CMV::RUBY::OCS.

   Depositor

   Chih-Hang Wu

   Article

   Chiang et al bioRxiv 2024.03.23.586378

   Insert

   CBS4-CMV1::RUBY::OCS
   

   Use

   Synthetic Biology

   Expression

   Plant

   Mutation

   no

   Available Since

   May 12, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pIVT-Bxb1

   Plasmid

   #232746

   Purpose

   IVT template for making modRNA encoding Bxb1

   Depositor

   Kate Galloway

   Article

   Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

   Insert

   Bxb1
   

   Use

   In vitro transcription

   Tags

   HA and SV40 NLS

   Available Since

   March 17, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. BCJJ345

   Plasmid

   #117504

   Purpose

   YAO:Cas9:E9 Golden Gate Level 1 Position 2 reverse

   Depositor

   Jonathan D Jones

   Article

   Castel et al PLoS One. 2019 Jan 9;14(1):e0204778. doi

   Insert

   BpiI:tagt:YAO:Cas9-3(intron):E9:ttgc:BpiI
   

   Use

   CRISPR

   Expression

   Plant

   Promoter

   AtYAO

   Available Since

   Nov. 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. MbCas12a_N1153_GPG_GR2

   Plasmid

   #231578

   Purpose

   Encodes a cortisol inducible variant of MbCas12a

   Depositor

   Dominik Niopek

   Article

   Wolf et al bioRxiv 2024.12.04.626757

   Insert

   MbCas12a with a GR2 insertion behind N1153
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Jan. 30, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pIVT-mGL

    Plasmid

    #232768

    Purpose

    IVT template for making modRNA encoding mGreenLantern

    Depositor

    Kate Galloway

    Article

    Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

    Insert

    mGreenLantern
    

    Use

    In vitro transcription

    Available Since

    March 17, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pICSL11015

    Plasmid

    #117499

    Purpose

    FAST-Red selectable marker Golden Gate Level 1 Position 1

    Depositor

    Jonathan D Jones

    Article

    Castel et al PLoS One. 2019 Jan 9;14(1):e0204778. doi

    Insert

    BpiI:TGCC:pOLE1:OLE1-RFP:OLE1t:GCAA:BpiI
    

    Tags

    RFP

    Expression

    Plant

    Promoter

    AtOLE1

    Available Since

    Oct. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pJJB1577

    Plasmid

    #218619

    Purpose

    Express Pex5, Pex2, Pex17, and Pex10

    Depositor

    John Dueber

    Article

    Baker et al Nat Chem Biol. 2024 Oct 14. doi: 10.1038

    Insert

    Pex5
    

    Expression

    Yeast

    Available Since

    Oct. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. MET-HYGRO

    Plasmid

    #218113

    Purpose

    Integration backbone for the MET15 integration site of S. cerevisiae. Includes a HygromycinR gene and a sfGFP drop-out.

    Depositor

    Carl Denard

    Article

    Martinusen et al Protein Eng Des Sel. 2024 Jan 29;37:gzae

    Type

    Empty backbone

    Expression

    Bacterial and Yeast

    Available Since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCAG-T7-TALEN(Sangamo)-FokI-ELD-Destination

    Plasmid

    #40132

    Purpose

    Destination vector for Voytas Golden Gate TALEN assembly incorporating a CAG promoter for mammalian expression and a T7 promoter for synthesis of TALEN mRNA. Uses heterodimeric FokI domain (ELD).

    Depositor

    Pawel Pelczar

    Article

    Hermann et al J Vis Exp. 2014 Apr 2;(86). doi: 10.3791

    Type

    Empty backbone

    Tags

    FokI nuclease (ELD)

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Aug. 31, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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15. pRO117

    Plasmid

    #233823

    Purpose

    MoClo-YTK; type3; Reporter; mNeonGreen

    Depositor

    Jeff Hasty

    Article

    O'Laughlin et al ACS Synth Biol. 2024 Jan 19;13(1):85-102

    Insert

    mNeonGreen
    

    Expression

    Yeast

    Available Since

    May 22, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCAG-T7-TALEN(Sangamo)-FokI-KKR-Destination

    Plasmid

    #40131

    Purpose

    Destination vector for Voytas Golden Gate TALEN assembly incorporating a CAG promoter for mammalian expression and a T7 promoter for synthesis of TALEN mRNA. Uses heterodimeric FokI domain (KKR).

    Depositor

    Pawel Pelczar

    Article

    Hermann et al J Vis Exp. 2014 Apr 2;(86). doi: 10.3791

    Type

    Empty backbone

    Tags

    FokI nuclease (KKR)

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Aug. 31, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCHW111117

    Plasmid

    #222460

    Purpose

    Level 1 acceptor, Position 3 containing CBS4-CMV1::LoxP-mCherry-stop-LacZ::nosT.

    Depositor

    Chih-Hang Wu

    Article

    Chiang et al bioRxiv 2024.03.23.586378

    Type

    Empty backbone

    Use

    Synthetic Biology

    Expression

    Plant

    Mutation

    no

    Promoter

    CBS4-CMV1 (Cucumber mosaic virus)

    Available Since

    Nov. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pPiggyBacATCC LacZ GCTT loxAttP

    Plasmid

    #173496

    Purpose

    piggyBac insect transgenesis destination vector for Golden Gate Cloning assemblies

    Depositor

    Eric Marois

    Article

    Lutrat et al Commun Biol. 2022 Mar 7;5(1):210. doi: 1

    Type

    Empty backbone

    Expression

    Insect

    Available Since

    Sept. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCHW110035

    Plasmid

    #222444

    Purpose

    Level 0, N-terminal tag module (CCAT-AATG) containing LoxP-mCherry-stop.

    Depositor

    Chih-Hang Wu

    Article

    Chiang et al bioRxiv 2024.03.23.586378

    Insert

    LoxP-mCherry-stop
    

    Use

    Synthetic Biology

    Expression

    Plant

    Mutation

    no

    Available Since

    Nov. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pYPQ146

    Plasmid

    #69298

    Purpose

    Golden Gate recipient and Gateway entry vector; assembly of 6 gRNAs

    Depositor

    Yiping Qi

    Article

    Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

    Type

    Empty backbone

    Use

    CRISPR

    Available Since

    Sept. 14, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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