We narrowed to 7,746 results for: sms

1. pSDMA66

   Plasmid

   #128355

   Purpose

   gRNA ribozyme construct with Cryptococcus neoformans ACT1 promter and TRP1 terminator. Following ribozyme cleavage, a gRNA targeting ADE2 is liberated.

   Depositor

   James Fraser

   Article

   Arras et al PLoS One. 2016 Oct 6;11(10):e0164322. do

   Insert

   Noureothricin (NAT)
   

   Use

   Unspecified

   Tags

   Expression

   Mutation

   Promoter

   ACT1

   Available since

   Aug. 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pLJM1-FLAG-Sestrin2-E451A

   Plasmid

   #73677

   Purpose

   overexpression

   Depositor

   David Sabatini

   Article

   Wolfson et al Science. 2016 Jan 1;351(6268):43-8. doi:

   Insert

   Sesn2 (SESN2 Human)
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   E451A changed Glutamate 451 to Alanine

   Promoter

   CMV

   Available since

   March 30, 2016

   Availability

   Academic Institutions and Nonprofits only

3. pFA6a-link-GFPγ-SpHis5

   Plasmid

   #60781

   Purpose

   Yeast C-terminal tagging vector, which creates gene fusions with GFPγ using SpHis5 selection

   Depositor

   Linda Huang

   Article

   Slubowski et al Yeast. 2015 Jan 22. doi: 10.1002/yea.306

   Insert

   GFPγ
   

   Use

   Yeast integrating

   Tags

   Expression

   Mutation

   T65C S72A V163A I167T L231H

   Promoter

   Available since

   Dec. 5, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCF711

   Plasmid

   #121662

   Purpose

   U6-sgRNA EFS-ProCas9-FlaviS6-P2A-Puro

   Depositor

   David Savage

   Article

   Oakes et al Cell. 2019 Jan 10;176(1-2):254-267.e16.

   Inserts

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available since

   March 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. HA Raptor mutant 7

   Plasmid

   #8475

   Depositor

   David Sabatini

   Article

   Kim et al Cell 2002 Jul 26;110(2):163-75.

   Insert

   Raptor (RPTOR Human)
   

   Use

   Tags

   ha

   Expression

   Mammalian

   Mutation

   738SLQN741-PAAA (S738P, L739A, Q740A, N741A)

   Promoter

   Available since

   May 11, 2005

   Availability

   Academic Institutions and Nonprofits only

6. Cherry-tTA-ER-C3

   Plasmid

   #134567

   Purpose

   Expresses a Cherry-tTA (tetracycline transcriptional activator)-ER (estrogen receptor hormone binding domain) fusion protein

   Depositor

   Susan Janicki

   Article

   Rafalska-Metcalf et al PLoS One. 2010 Apr 21;5(4):e10272. doi:

   Insert

   tTA-ER
   

   Use

   Tags

   Cherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pALPS_3'LTR GFP@gag start SFFV rev

   Plasmid

   #101339

   Purpose

   LTR drives GFP and internal SFFV promoter drives codon optimized rev

   Depositor

   Jeremy Luban

   Article

   McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:

   Insert

   GFP, rev
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. F5A deltaCT S6K1 MSCV puro

   Plasmid

   #8464

   Depositor

   David Sabatini

   Article

   Ali et al J Biol Chem 2005 Apr 4;.

   Insert

   f5a delta ctp70 S6K1 (Rps6kb1 Rat)
   

   Use

   Retroviral

   Tags

   myc

   Expression

   Mammalian

   Mutation

   deletion of 104 c-terminal amino acids mutation …

   Promoter

   Available since

   April 4, 2006

   Availability

   Academic Institutions and Nonprofits only

9. pAcrF3

   Plasmid

   #89246

   Purpose

   Plasmid contains the gene for anti-CRISPR protein AcrF3 (gene product 35 from bacteriophage JBD5).

   Depositor

   Blake Wiedenheft

   Article

   Rollins et al Proc Natl Acad Sci U S A. 2017 Apr 24. p

   Insert

   AcrF3
   

   Use

   Tags

   6xHis-TEV

   Expression

   Mutation

   Promoter

   Available since

   April 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. Mis12 INCENP Δcen mCherry

    Plasmid

    #45228

    Depositor

    Susanne Lens, Michael Lampson

    Article

    Liu et al Science. 2009 Mar 6;323(5919):1350-3. do

    Insert

    Mis12 INCENP Δcen mCherry
    

    Use

    Tags

    VSVG

    Expression

    Mammalian

    Mutation

    CEN box deleted from INCENP (aa1-46)

    Promoter

    Available since

    July 16, 2013

    Availability

    Academic Institutions and Nonprofits only

11. pCF710

    Plasmid

    #121892

    Purpose

    EF1a-WNV-protease-mTagBFP2

    Depositor

    David Savage

    Article

    Oakes et al Cell. 2019 Jan 10;176(1-2):254-267.e16.

    Inserts

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EF1a core promoter

    Available since

    March 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pSDMA67

    Plasmid

    #128356

    Purpose

    gRNA ribozyme construct with Cryptococcus neoformans ACT1 promter and TRP1 terminator. Following ribozyme cleavage, a gRNA targeting ADE2 is liberated.

    Depositor

    James Fraser

    Article

    Arras et al PLoS One. 2016 Oct 6;11(10):e0164322. do

    Insert

    Noureothricin (NAT)
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    ACT1

    Available since

    Aug. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. env8 selectivity (d')

    Plasmid

    #99838

    Purpose

    mNeonGreen reporter that places expression of a fluorescent protein reporter (mNeonGreen) under control of the env8HyCbl riboswitch.

    Depositor

    Robert Batey

    Article

    Polaski et al J Biol Chem. 2017 May 8. pii: jbc.M117.7

    Insert

    env8 selectivity (d')
    

    Use

    Tags

    mNeonGreen

    Expression

    Bacterial

    Mutation

    A20G, A68G, G-C pairs in P1 and P3, G12A, G10U de…

    Promoter

    Available since

    April 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pTU1-A-lacZ

    Plasmid

    #72935

    Purpose

    Level 1 Destination vector - Position A

    Depositor

    Paul Freemont

    Article

    Moore et al ACS Synth Biol. 2016 May 2.

    Insert

    Level 1 - A site
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Beta-lactamase - G717C (nucleotide) - silent muta…

    Promoter

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. GB1-His-SAP25-126-186(pL91)

    Plasmid

    #124387

    Purpose

    E.Coli produced SAP25 SID purified by FPLC nickel column

    Depositor

    Shaun Cowley

    Article

    Chandru et al Sci Rep. 2018 Oct 2;8(1):14689. doi: 10.

    Insert

    GB1-His-SAP25-126-186 (SAP25 Human)
    

    Use

    Tags

    GB1-Histidine

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. HA Raptor mutant 2

    Plasmid

    #8471

    Depositor

    David Sabatini

    Article

    Kim et al Cell 2002 Jul 26;110(2):163-75.

    Insert

    Raptor (RPTOR Human)
    

    Use

    Tags

    ha

    Expression

    Mammalian

    Mutation

    261DLF263-AAA (D261A, L262A, F263A)

    Promoter

    Available since

    May 11, 2005

    Availability

    Academic Institutions and Nonprofits only

17. pCF713

    Plasmid

    #121665

    Purpose

    U6-sgRNA EF1a-ProCas9-FlaviS6-P2A-Puro

    Depositor

    David Savage

    Article

    Oakes et al Cell. 2019 Jan 10;176(1-2):254-267.e16.

    Inserts

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    March 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. HA Raptor mutant 3

    Plasmid

    #8473

    Depositor

    David Sabatini

    Article

    Kim et al Cell 2002 Jul 26;110(2):163-75.

    Insert

    Raptor (RPTOR Human)
    

    Use

    Tags

    ha

    Expression

    Mammalian

    Mutation

    313NWIF316-AAAA (N313A, W314A, I315A, F316A)

    Promoter

    Available since

    May 11, 2005

    Availability

    Academic Institutions and Nonprofits only

19. pALPS_3'LTR GFP@gag start SFFV tat

    Plasmid

    #101338

    Purpose

    LTR drives GFP and internal SFFV promoter drives codon optimized tat

    Depositor

    Jeremy Luban

    Article

    McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:

    Insert

    GFP, tat
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pJZ002

    Plasmid

    #120231

    Purpose

    CRISPR-cas9 targeting in E. coli with ampicillin resistance

    Depositor

    Bradley Moore

    Article

    Bauman et al Cell Chem Biol. 2019 Feb 15. pii: S2451-

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge