Addgene

We narrowed to 142 results for: SYFP2

Showing: 1 - 20 of 142 results

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pRJPaph-sYFP2

Plasmid

#67033

Purpose

Plasmid for Paph-sYFP2 integration downstream of B. diazoefficiens (B. japonicum) scoI

Depositor

Julia Vorholt

Article

Ledermann et al Mol Plant Microbe Interact. 2015 Jun 2.

Inserts

B. diazoefficiens DNA from scoI downstream region (comprising blr1132')
sYFP2

Use

Conjugative bacterial vector

Tags

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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BtGRK2-sYFP2

Plasmid

#137778

Purpose

Visualization of GRK2

Depositor

Dorus Gadella

Article

Pietraszewska-Bogiel et al ACS Omega. 2020 Feb 3;5(6):2648-2659. do

Insert

Bovine GRK2 (GRK2 Bovine)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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pDx_mRuby3-SYFP2

Plasmid

#189758

Purpose

Dual expression vector for bacteria and mammalian cells producing mRuby3-SYFP2 fusion protein for FRET analysis

Depositor

Dorus Gadella

Article

Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi

Insert

mRuby3-SYFP2

Use

Tags

6xHis

Expression

Bacterial and Mammalian

Mutation

Promoter

CMV & Rha

Availability

Academic Institutions and Nonprofits only

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pDx_mCherry-SYFP2

Plasmid

#189759

Purpose

Dual expression vector for bacteria and mammalian cells producing mCherry-SYFP2 fusion protein for FRET analysis

Depositor

Dorus Gadella

Article

Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi

Insert

mCherry-SYFP2

Use

Tags

6xHis

Expression

Bacterial and Mammalian

Mutation

Promoter

CMV & Rha

Availability

Academic Institutions and Nonprofits only

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pDx_FusionRed-SYFP2

Plasmid

#189760

Purpose

Dual expression vector for bacteria and mammalian cells producing FusionRed-SYFP2 fusion protein for FRET analysis

Depositor

Dorus Gadella

Article

Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi

Insert

FusionRed-SYFP2

Use

Tags

6xHis

Expression

Bacterial and Mammalian

Mutation

Promoter

CMV & Rha

Availability

Academic Institutions and Nonprofits only

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pDx_mScarlet-SYFP2

Plasmid

#189762

Purpose

Dual expression vector for bacteria and mammalian cells producing mScarlet-SYFP2 fusion protein for FRET analysis

Depositor

Dorus Gadella

Article

Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi

Insert

mScarlet-SYFP2

Use

Tags

6xHis

Expression

Bacterial and Mammalian

Mutation

Promoter

CMV & Rha

Availability

Academic Institutions and Nonprofits only

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pDx_mScarlet3-SYFP2

Plasmid

#189764

Purpose

Dual expression vector for bacteria and mammalian cells producing mScarlet3-SYFP2 fusion protein for FRET analysis

Depositor

Dorus Gadella

Article

Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi

Insert

mScarlet3-SYFP2

Use

Tags

6xHis

Expression

Bacterial and Mammalian

Mutation

Promoter

CMV & Rha

Availability

Academic Institutions and Nonprofits only

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pSYFP2-C1

Plasmid

#22878

Depositor

Dorus Gadella

Article

Kremers et al Biochemistry. 2006 May 30. 45(21):6570-8

Insert

SYFP2

Use

Tags

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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PH-PLCD1_mScarlet_IRES_SYFP2_PH_N1

Plasmid

#85069

Purpose

Yellow/red FRET biosensor for monotoring plasmamembrane PtdIns(4,5)P2 levels (PIP2)

Depositor

Dorus Gadella

Article

Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

Insert

PH-PLCD1 (PLCD1 Human)

Use

Tags

IRES-sYFP2-PH and mScarlet

Expression

Mammalian

Mutation

PLCD aa 1–170

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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cfSYFP2-N

Plasmid

#110631

Purpose

Cys-free yellow fluorescent protein for extracellular imaging. SYFP2 variant (C48S/C70M). Molecular brightness is almost comparable to SYFP2.

Depositor

Ikuo Wada

Insert

cfSYFP2-N

Use

Tags

Multi-cloning sites

Expression

Mammalian

Mutation

Cys48Ser and Cys70/Met

Promoter

Availability

Academic Institutions and Nonprofits only

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pSYFP2-SCFP3A

Plasmid

#22905

Depositor

Dorus Gadella

Article

Kremers et al Biochemistry. 2006 May 30. 45(21):6570-8

Insert

SYFP2-SCFP3A

Use

Tags

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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PH-PLCD1_mScarlet-H_IRES_SYFP2_PH_N1

Plasmid

#85070

Purpose

Yellow/red FRET biosensor for monotoring plasmamembrane PtdIns(4,5)P2 levels (PIP2)

Depositor

Dorus Gadella

Article

Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

Insert

PH-PLCD1 (PLCD1 Human)

Use

Tags

IRES-sYFP2-PH and mScarlet-H

Expression

Mammalian

Mutation

PLCD aa 1–170

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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PH-PLCD1_mScarlet-I_IRES_sYFP2_PH_N1

Plasmid

#110623

Purpose

It expresses separate mScarletI pHdomain and SYFP2 pHdomain in a 3:1 ratio.

Depositor

Dorus Gadella

Article

Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

Insert

PH mScarlet-I- IRES- sYFP2 PH

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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pDx_mScarlet-I-SYFP2

Plasmid

#189761

Purpose

Dual expression vector for bacteria and mammalian cells producing mScarlet-I-SYFP2 fusion protein for FRET analysis

Depositor

Dorus Gadella

Article

Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi

Insert

mScarlet-I-SYFP2

Use

Tags

6xHis

Expression

Bacterial and Mammalian

Mutation

Promoter

CMV & Rha

Availability

Academic Institutions and Nonprofits only

Enlarge
pDx_mScarlet-I3-SYFP2

Plasmid

#189763

Purpose

Dual expression vector for bacteria and mammalian cells producing mScarlet-I3-SYFP2 fusion protein for FRET analysis

Depositor

Dorus Gadella

Article

Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi

Insert

mScarlet-I3-SYFP2

Use

Tags

6xHis

Expression

Bacterial and Mammalian

Mutation

Promoter

CMV & Rha

Availability

Academic Institutions and Nonprofits only

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sYFP2-OP18-mScI

Plasmid

#128003

Purpose

Redshifted FRET-readout of Stathmin/OP18 interaction with α-tubulin

Depositor

Dorus Gadella

Article

Van Geel et al Sci Rep. 2020 Apr 7;10(1):6034. doi: 10.

Insert

sYFP2-OP18-mScI

Use

Tags

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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ROPY-sYFP2(Y67G)

Plasmid

#128008

Purpose

Dark sYFP2 mutant of ROPY

Depositor

Dorus Gadella

Article

Van Geel et al Sci Rep. 2020 Apr 7;10(1):6034. doi: 10.

Insert

ROPY-sYFP2(Y67G)

Use

Tags

Expression

Mammalian

Mutation

Y67G in sYFP2

Promoter

Availability

Academic Institutions and Nonprofits only

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pRepair-SYFP2-CTNNB1

Plasmid

#153432

Purpose

Homology Directed Repair construct for N-terminal tagging of hsCTNNB1 with SYFP2

Depositor

Renee van Amerongen

Article

de Man et al Elife. 2021 Jun 30;10. pii: 66440. doi:

Insert

CTNNB1 homology arms and SYFP2 coding sequence (CTNNB1 Human)

Use

CRISPR; Hdr donor template

Tags

SYFP2

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pDx_mScarlet-I3-NCwt-SYFP2

Plasmid

#189765

Purpose

Dual expression vector for bacteria and mammalian cells producing mScarlet-I3-NCwt-SYFP2 fusion protein for FRET analysis

Depositor

Dorus Gadella

Article

Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi

Insert

mScarlet-I3-NCwt-SYFP2

Use

Tags

6xHis

Expression

Bacterial and Mammalian

Mutation

Promoter

CMV & Rha

Availability

Academic Institutions and Nonprofits only

Enlarge
pDx_mScarlet3-NCwt-SYFP2

Plasmid

#189766

Purpose

Dual expression vector for bacteria and mammalian cells producing mScarlet3-NCwt-SYFP2 fusion protein for FRET analysis

Depositor

Dorus Gadella

Article

Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi

Insert

mScarlet3-NCwt-SYFP2

Use

Tags

6xHis

Expression

Bacterial and Mammalian

Mutation

encodes the expected internal mutations relative …

Promoter

CMV & Rha

Availability

Academic Institutions and Nonprofits only

Enlarge