Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

We narrowed to 64 results for: golden gate

Clear keywords
Showing: 21 - 40 of 64 results

  1. 4G-Cloning Plasmid Kit

    Plasmid Kit
    #1000000252
    Purpose
    Use 4G (Golden-Gate-guided Gibson) cloning for rapid assembly of single- or multi-gene expression vectors for multi-subunit protein complexes in various expression hosts.
    Depositor
    Stephan Gruber
    Application
    Cloning and Synthetic Biology
    Vector Type
    Bacterial Expression
    Cloning Type
    Golden Gate
    Available Since
    Jan. 21, 2025
    Availability
    Academic Institutions and Nonprofits only

  2. AspFlex Kit

    Plasmid Kit
    #1000000217
    Purpose
    Collection of standardized parts for protein expression analyses and CRISPRi experiments in Acinetobacter sp.
    Depositor
    Veronica Godoy-Carter
    Application
    Cloning and Synthetic Biology
    Vector Type
    Bacterial Expression
    Cloning Type
    Golden Gate (MoClo)
    Available Since
    Sept. 27, 2023
    Availability
    Academic Institutions and Nonprofits only

  3. LbPlant toolbox I

    Plasmid Kit
    #1000000262
    Purpose
    Plasmids and parts for hierarchical assembly for expression in Lactiplantibacillus plantarum. Recommended to be used in combination with LbPlant Toolbox II.
    Depositor
    Thorsten Mascher
    Application
    Cloning and Synthetic Biology
    Vector Type
    Bacterial Expression
    Cloning Type
    Golden Gate (MoClo)
    Available Since
    July 22, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits

  4. MoClo Yeast Secretion and Display (YSD) Toolkit

    Plasmid Kit
    #1000000240
    Purpose
    Collection of plasmids to optimize secretion and cell surface display of proteins of interest in yeast.
    Depositor
    Paul Young
    Application
    Cloning and Synthetic Biology
    Vector Type
    Yeast Expression
    Cloning Type
    Golden Gate (MoClo)
    Available Since
    April 10, 2024
    Availability
    Academic Institutions and Nonprofits only

  5. Ralstonia-GG-Kit

    Plasmid Kit
    #1000000142
    Purpose
    Plasmids for gene knockout and complementation in the bacterial plant pathogen Ralstonia solanacearum.
    Depositor
    Thomas Lahaye
    Application
    Cloning and Synthetic Biology
    Vector Type
    Bacterial Expression
    Cloning Type
    Golden Gate
    Available Since
    Dec. 18, 2018
    Availability
    Academic Institutions and Nonprofits only

  6. POMBOX MoClo YTK

    Plasmid Kit
    #1000000251
    Purpose
    Seamless and efficient assembly of single gene and multigene plasmids for genome integration in Schizosaccharomyces pombe.
    Depositor
    Tomáš Pluskal
    Application
    Cloning and Synthetic Biology
    Vector Type
    Yeast Expression
    Cloning Type
    Golden Gate (MoClo)
    Available Since
    Jan. 23, 2025
    Availability
    Academic Institutions and Nonprofits only

  7. Yeast GPCR-sensor Toolkit

    Plasmid Kit
    #1000000157
    Purpose
    This toolkit includes plasmids for constructing highly-tunable GPCR-based biosensors in yeast.
    Depositor
    Tom Ellis
    Application
    Cloning and Synthetic Biology, Gene Expression and Labeling
    Vector Type
    Yeast Expression
    Cloning Type
    Golden Gate (MoClo)
    Available Since
    Oct. 17, 2019
    Availability
    Academic Institutions and Nonprofits only

  8. MultiGreen

    Plasmid Kit
    #1000000250
    Purpose
    Modular assembly of multi-gene expression vectors.
    Depositor
    Wayne Parrott
    Application
    Cloning and Synthetic Biology
    Vector Type
    Plant Expression
    Cloning Type
    Golden Gate (GreenGate)
    Available Since
    Dec. 16, 2024
    Availability
    Academic Institutions and Nonprofits only

  9. MoBiFC

    Plasmid Kit
    #1000000222
    Purpose
    Modular bimolecular fluorescence complementation (MoBiFC) toolkit for analyzing protein-protein interactions in plants.
    Depositor
    Benjamin Field
    Application
    Cloning and Synthetic Biology, Gene Expression and Labeling
    Vector Type
    Plant Expression
    Cloning Type
    Golden Gate (MoClo)
    Available Since
    July 26, 2024
    Availability
    Academic Institutions and Nonprofits only

  10. Komagataeibacter Tool Kit (KTK)

    Plasmid Kit
    #1000000216
    Purpose
    A collection of standardized parts used for hierarchical assembly of single and multi-gene constructs for Komagataeibacter species.
    Depositor
    Tom Ellis
    Application
    Cloning and Synthetic Biology
    Vector Type
    Bacterial Expression
    Cloning Type
    Golden Gate (MoClo)
    Available Since
    May 2, 2023
    Availability
    Academic Institutions and Nonprofits only

  11. Start-Stop Assembly Toolkit

    Plasmid Kit
    #1000000152
    Purpose
    Modular, scarless DNA assembly method suitable for combinatorial assembly and ideal for metabolic engineering.
    Depositor
    John Heap
    Application
    Cloning and Synthetic Biology
    Vector Type
    Bacterial Expression
    Cloning Type
    Golden Gate
    Available Since
    March 20, 2019
    Availability
    Academic Institutions and Nonprofits only

  12. EMMA toolkit

    Plasmid Kit
    #1000000119
    Purpose
    Collection of Golden Gate vectors and standardized genetic parts for the construction mammalian expression plasmids.
    Depositor
    Yizhi Cai
    Application
    Cloning and Synthetic Biology
    Vector Type
    Mammalian Expression
    Cloning Type
    Golden Gate
    Available Since
    March 1, 2018
    Availability
    Academic Institutions and Nonprofits only

  13. MoClo Baculo toolkit

    Plasmid Kit
    #1000000256
    Purpose
    Plasmids to construct multigene expression vectors for yeast and baculovirus, through compatibility with the Bac-to-Bac and biGBac systems.
    Depositor
    Chen Davidovich
    Application
    Cloning and Synthetic Biology
    Vector Type
    Insect Expression, Yeast Expression
    Cloning Type
    Golden Gate (MoClo)
    Available Since
    May 13, 2025
    Availability
    Academic Institutions and Nonprofits only

  14. Easy-MISE toolkit

    Plasmid Kit
    #1000000230
    Purpose
    Easy-Modular Integrative fuSion-ready Expression toolkit allows fast metabolic engineering in Saccharomyces cerevisiae using CRISPR-Cas9 to insert TUs into well-defined genomic loci.
    Depositor
    Paola Branduardi
    Application
    Cloning and Synthetic Biology, Genome Editing
    Vector Type
    Yeast Expression
    Editing Type
    CRISPR
    Cloning Type
    Golden Gate
    Available Since
    Oct. 25, 2023
    Availability
    Academic Institutions and Nonprofits only

  15. yeast Tunable Expression Systems Toolkit (yTEST)

    Plasmid Kit
    #1000000266
    Purpose
    Plasmids for assembly of small-molecule inducible promoter systems in Saccharomyces cerevisiae.
    Depositor
    Jeff Hasty
    Application
    Cloning and Synthetic Biology
    Vector Type
    Yeast Expression
    Cloning Type
    Golden Gate (MoClo)
    Available Since
    Aug. 19, 2025
    Availability
    Academic Institutions and Nonprofits only

  16. Yeast Secrete and Detect

    Plasmid Kit
    #1000000166
    Purpose
    Assembly of transcription units for protein secretion in the yeasts Saccharomyces cerevisiae and Pichia pastoris.
    Depositor
    Sylvestre Marillonnet
    Application
    Cloning and Synthetic Biology
    Vector Type
    Yeast Expression
    Cloning Type
    Golden Gate (MoClo)
    Available Since
    Jan. 28, 2021
    Availability
    Academic Institutions and Nonprofits only

  17. openCIDAR MoClo Toolkit

    Plasmid Kit
    #1000000227
    Purpose
    A collection of standardized parts compatible with CIDAR MoClo (Addgene #1000000059) to assemble gene expression constructs in many bacteria across Pseudomonadota.
    Depositor
    Eric Young
    Application
    Cloning and Synthetic Biology
    Vector Type
    Bacterial Expression
    Cloning Type
    Golden Gate (MoClo)
    Available Since
    Sept. 20, 2023
    Availability
    Academic Institutions and Nonprofits only

  18. PUF Assembly Kit

    Plasmid Kit
    #1000000051
    Purpose
    Assembly of Pumilio and FBF (PUF) homology domain based RNA-binding proteins for recognition of RNA targets.
    Depositor
    Huimin Zhao
    Application
    Cloning and Synthetic Biology, Genome Editing
    Vector Type
    Bacterial Expression, Mammalian Expression
    Editing Type
    PUF
    Cloning Type
    Golden Gate
    Available Since
    June 17, 2014
    Availability
    Academic Institutions and Nonprofits only

  19. TUNEYALI Kit

    Plasmid Kit
    #1000000255
    Purpose
    Regulate gene expression by combining gRNA constructs targeting natural promoters and insertion of synthetic promoters in Yarrowia lipolytica.
    Depositor
    Irina Borodina
    Application
    Cloning and Synthetic Biology, Genome Editing
    Vector Type
    Yeast Expression
    Editing Type
    CRISPR
    Cloning Type
    Golden Gate
    Available Since
    April 28, 2025
    Availability
    Academic Institutions and Nonprofits only

  20. MoClo Yeast Secretion Biosensor (YSB) Toolkit

    Plasmid Kit
    #1000000268
    Purpose
    Library of plasmids containing a variety of signal peptides, C-tags, and alpha-mating factor tags for use in protein secretion optimization in yeast.
    Depositor
    Joseph Brock
    Application
    Cloning and Synthetic Biology
    Vector Type
    Yeast Expression
    Cloning Type
    Golden Gate (MoClo)
    Available Since
    Sept. 17, 2025
    Availability
    Academic Institutions and Nonprofits only
Showing: 21 - 40 of 64 results