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pET22b-T7-6h-GST-α-Synuclein

Plasmid

#225224

Purpose

Alpha synuclein gene fused with gst gene under t7 promoter for bacterial expression of alpha synuclein protein.

Depositor

Urartu Seker

Article

Ozcelik et al ACS Chem Neurosci. 2023 Oct 4;14(19):360

Inserts

GST (GSTK1 E. coli)
α-Synuclein (SNCA Human)

Tags

6xHis Tag and TEV Cleavage Site

Expression

Bacterial

Promoter

T7

Available Since

Feb. 5, 2025

Availability

Academic Institutions and Nonprofits only

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pTec_mCherry

Plasmid

#229145

Purpose

Promoter strength evaluation of Ptec

Depositor

Shrikrishnan Sankaran

Article

Blanch-Asensio et al Microb Cell Fact. 2024 Feb 7;23(1):42. d

Insert

mCherry

Expression

Bacterial

Mutation

No

Promoter

Ptec

Available Since

Jan. 29, 2025

Availability

Academic Institutions and Nonprofits only

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pET-30a-L6KD-PT linker-PEP-Mtu ΔI-CM-xylanase

Plasmid

#231128

Purpose

Expresses xylanase via a cSAT2.0 scheme

Depositor

Xiaofeng Yang

Article

Huang et al Trends Biotechnol. 2024 Nov 12:S0167-779

Insert

xylanase

Tags

cSAT2.0

Expression

Bacterial

Promoter

T7

Available Since

Jan. 29, 2025

Availability

Academic Institutions and Nonprofits only

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pET28a-AlkB

Plasmid

#228218

Purpose

Express wild type AlkB protein in E. coli BL21(DE3)

Depositor

Junchao Shi

Article

Shi et al Nat Cell Biol. 2021 Apr;23(4):424-436. d

Insert

Alpha-ketoglutarate-dependent Dioxygenase (alkB Synthetic)

Tags

His tag

Expression

Bacterial

Mutation

codon optimized

Promoter

T7

Available Since

Jan. 28, 2025

Availability

Academic Institutions and Nonprofits only

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pVP141

Plasmid

#222079

Purpose

Single component CRISPR-mediated base-editor for Agrobacterium genetic engineering visually marked with sfGFP for guide insertion.

Depositor

Wayne Parrott

Article

Pennetti et al Biodes Res. 2025 Feb 27;7(1):100001. doi

Insert

dCas9-PmCDA1, sfGFP and sacB

Use

CRISPR

Expression

Bacterial

Available Since

Jan. 27, 2025

Availability

Academic Institutions and Nonprofits only

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pRSFDUET-1_VNp-anti-6xHis mAb

Plasmid

#214952

Purpose

Bacterial expression of anti-6xHis mAb light and heavy chain VNp fusions.

Depositor

Dan Mulvihill

Article

Baker et al Open Biol. 2025 Feb;15(2):240229. doi: 1

Inserts

VNp-anti-6xHis mAb heavy chain
VNp-anti-6xHis mAb light chain

Tags

VNp6

Expression

Bacterial

Promoter

T7

Available Since

Jan. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pET21-pelB-LaG94-15

Plasmid

#228971

Purpose

For bacterial expression of anti-GFP nanobody LaG94-15, with pelB leader for periplasmic secretion, and C-term free cysteine and 6xHIS tag.

Depositor

Michael Rout

Article

Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

Insert

LaG94-15

Tags

6xHIS

Expression

Bacterial

Promoter

T7

Available Since

Jan. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pKJ0463

Plasmid

#228715

Purpose

Bacterial expression of epT7 RNAP; for protein production and purification

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Article

Jiang et al Science 0, eadr6006

Insert

Sumo_epT7

Expression

Bacterial

Mutation

T3M/G47A/E643G

Available Since

Nov. 25, 2024

Availability

Academic Institutions and Nonprofits only

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pGEX-parkin

Plasmid

#227367

Purpose

For bacterial expression of parkin

Depositor

Kalle Gehring

Article

Huq et al Commun Biol. 2024 Aug 8;7(1):961. doi: 1

Insert

Parkin (PRKN Human)

Tags

GST

Expression

Bacterial

Mutation

Codon usage optimized for E.coli expression.

Available Since

Nov. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pCF1135

Plasmid

#222106

Purpose

E. coli - Veillonella shuttle plasmid with single selectable marker

Depositor

J Christopher Fenno

Article

Goetting-Minesky et al Lett Appl Microbiol. 2024 Aug 5;77(8):ov

Type

Empty backbone

Use

E. coli-veillonella shuttle plasmid

Expression

Bacterial

Available Since

Oct. 31, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pCF1148

Plasmid

#222330

Purpose

Fluorescent reporter plasmid for Veillonella

Depositor

J Christopher Fenno

Article

Goetting-Minesky et al Lett Appl Microbiol. 2024 Aug 5;77(8):ov

Insert

bs2 gene with Veillonella parvula SKV38 mdh promoter

Use

E. coli-veillonella shuttle plasmid

Expression

Bacterial

Promoter

Veillonella parvula SKV38 mdh promoter

Available Since

Oct. 31, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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WT USP7 catalytic

Plasmid

#227201

Purpose

Expresses human USP7 Catalytic domain with a N-terminal cleavable 6His tag in E. coli

Depositor

Irina Bezsonova

Article

Valles et al bioRxiv [Preprint]. 2023 Jan 12:2023.01.

Insert

USP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)

Tags

6His

Expression

Bacterial

Mutation

aa 207-560

Promoter

T7-promoter

Available Since

Oct. 17, 2024

Availability

Academic Institutions and Nonprofits only

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pDIV643

Plasmid

#226182

Purpose

Expresses a color marker (uidA) cassette for disruption on KU70 gene in K. phaffii.

Depositor

Uffe Mortensen

Article

Strucko et al FEMS Yeast Res. 2024 Aug 23:foae026. doi

Insert

uidA marker flanked with targeting regions to KU70 gene in K.phaffii

Expression

Bacterial

Promoter

TEF1p

Available Since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pBAD_FLAG-GFP(Y39TAG)-6His

Plasmid

#223510

Purpose

Fluorescent reporter plasmid for genetic code expansion which consists of a modified GFP with a Amber stop codon on position Y39

Depositor

Edward Lemke

Article

Milles et al J Am Chem Soc. 2012 Mar 21;134(11):5187-

Insert

GFP(Y39TAG)

Tags

6xHis and FLAG

Expression

Bacterial

Mutation

Y39TAG

Available Since

Oct. 7, 2024

Availability

Academic Institutions and Nonprofits only

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pQE81XN-mStayGold(J)

Plasmid

#225445

Purpose

Expresses Hisx6-Tagged mStayGold(J) in bacteria

Depositor

Benjamin Glick

Insert

mStayGold(J)

Tags

6X-His

Expression

Bacterial

Available Since

Sept. 20, 2024

Availability

Academic Institutions and Nonprofits only

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pBAD-iPASnFR-L

Plasmid

#225238

Purpose

Bacterial expression of iPASnFR-L

Depositor

Loren Looger

Article

Tamura et al bioRxiv 2024.08.21.609037

Insert

iPASnFR-L

Tags

6xHis

Expression

Bacterial

Available Since

Sept. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pFW2000

Plasmid

#224211

Purpose

E. coli-Bacteroides shuttle plasmid; pB8-51 (Bacteroides replication origin), p15A (E. coli replication origin)

Depositor

Ophelia Venturelli

Article

Feng et al Cell Host Microbe. 2022 Feb 9;30(2):200-

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available Since

Sept. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pBAD02_CBASS_Escherichia_coli_SLK172

Plasmid

#224395

Purpose

For expression of the type I CBASS system encoded by Escherichia coli SLK172

Depositor

Philip Kranzusch

Article

Richmond-Buccola et al Cell Host Microbe. 2024 Jul 10;32(7):107

Insert

EcCdnD_4TM

Expression

Bacterial

Available Since

Sept. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pQ-NFY-A-GB-HC

Plasmid

#171081

Purpose

Co-expresses of NFY complex in bacteria. The resulting plasmid was used to generate a single expression construct encoding the three subunit NF-Y, with untagged NFYA, GST-NFYB, and 6-His-NFYC

Depositor

Joan Conaway

Article

He et al Proc Natl Acad Sci U S A. 2021 Sep 28;11

Inserts

NF-YA (NFYA Human)
NF-YB (NFYB Human)
NF-YC (NFYC Human)

Tags

GST and His

Expression

Bacterial

Promoter

T5 promoter

Available Since

July 19, 2024

Availability

Academic Institutions and Nonprofits only

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pJMP3665

Plasmid

#222351

Purpose

Expression vector with PabstBR promoter, hygR marker, and replicates in A. baumannii and E. coli; empty vector plasmid with MCS for cloning - we recommend NcoI/BamHI.

Depositor

Jason Peters

Article

Bacon et al Microbiol Spectr. 2024 Nov 5;12(11):e013

Type

Empty backbone

Expression

Bacterial

Available Since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

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