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pBbA8c-RFP

Plasmid

#35272

Depositor

Jay Keasling

Article

Lee et al J Biol Eng. 2011 Sep 20;5:12.

Insert

RFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

BAD

Available Since

Feb. 16, 2012

Availability

Academic Institutions and Nonprofits only

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pSrpR-S3

Plasmid

#74693

Purpose

SrpR-S3 gate

Depositor

Christopher Voigt

Article

Nielsen et al Science. 2016 Apr 1;352(6281):aac7341. d

Insert

PTac-RBSS3-SrpR-PSrpR-YFP

Expression

Bacterial

Available Since

April 22, 2016

Availability

Academic Institutions and Nonprofits only

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pET23-PBD(65-150)-N-Cys

Plasmid

#13722

Depositor

Klaus Hahn

Article

Kraynov et al Science. 2000 Oct 13. 290(5490):333-7.

Insert

PBD(65-150)-N-Cys (PAK1 Human)

Tags

6xHis

Expression

Bacterial

Mutation

Met-Cys-Gly-PBD(65-150)-

Available Since

March 28, 2007

Availability

Academic Institutions and Nonprofits only
pET28b-ETV6-ETS

Plasmid

#111180

Purpose

Minimal ETS domain of human ETV6 (TEL)

Depositor

Gregory Poon

Article

Vo et al J Biol Chem. 2017 Aug 11;292(32):13187-1

Insert

ETV6 ETS domain (ETV6 Mouse, Bovine, Human)

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

Feb. 15, 2019

Availability

Academic Institutions and Nonprofits only

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PdnaK-IR3-IR3-Bxb1-CadR-pZa

Plasmid

#204686

Purpose

Cadmium responsive genetic circuit

Depositor

Urartu Seker

Article

Akboga et al Biosensors (Basel). 2022 Feb 16;12(2):12

Insert

Bxb1 (Bxb1p45 Synthetic)

Use

Synthetic Biology

Tags

CadR

Expression

Bacterial

Promoter

Inducible, HspR is its repressor

Available Since

Nov. 27, 2023

Availability

Academic Institutions and Nonprofits only

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5W9Q (MBD1)

Plasmid

#101265

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Cheryl Arrowsmith

Insert

MBD1 (MBD1 Human)

Tags

6xHis-TEV

Expression

Bacterial

Promoter

T7

Available Since

Oct. 2, 2017

Availability

Industry, Academic Institutions, and Nonprofits

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pCas_5+7_gfp

Plasmid

#60595

Purpose

encodes a reversed Int7 gene flanked by the attB/P sites of Int5, as well as a reversed GFP (GFPmut33) gene flanked by the attB/P sites of Int7.

Depositor

Christopher Voigt

Article

Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

Insert

Cas_5+7_gfp

Expression

Bacterial

Available Since

April 28, 2015

Availability

Academic Institutions and Nonprofits only

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pCas_2+5

Plasmid

#60594

Purpose

encodes the Int2 gene controlled by PBad promoter and a reversed Int5 gene flanked by the attB/P sites of Int2

Depositor

Christopher Voigt

Article

Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

Insert

Cas_2+5

Expression

Bacterial

Available Since

April 28, 2015

Availability

Academic Institutions and Nonprofits only

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pDB88

Plasmid

#26958

Depositor

Michael Glickman

Article

Barkan et al Gene. 2011 Jan 1;470(1-2):31-6. doi: 10.

Insert

e. coli galactokinase

Expression

Bacterial

Mutation

cloned under the MOP promoter

Available Since

March 18, 2011

Availability

Academic Institutions and Nonprofits only

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pMJ915v2 + sfGFP

Plasmid

#88918

Purpose

For expression of modified SpyCas9 in e.coli.

Depositor

Jennifer Doudna

Article

Staahl et al Nat Biotechnol. 2017 Feb 13. doi: 10.103

Inserts

Cas9
T7

Use

CRISPR

Expression

Bacterial

Available Since

May 2, 2017

Availability

Academic Institutions and Nonprofits only

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pBTK509

Plasmid

#110610

Purpose

BTK assembled broad-host-range plasmid encoding GFP driven by PA2 promoter

Depositor

Jeffrey Barrick

Article

Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

Insert

GFP

Expression

Bacterial

Available Since

Aug. 21, 2018

Availability

Industry, Academic Institutions, and Nonprofits

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pOPTM p62 PB1 (1-102)

Plasmid

#210427

Purpose

Bacterial expression of MBP-PB1(1-102) with TEV cleavage site

Depositor

Carsten Sachse

Article

Tarafder et al Methods Mol Biol. 2019;1880:3-15. doi: 1

Insert

p62 PB1 (1-102) (NUP62 Human)

Tags

MBP

Expression

Bacterial

Available Since

Jan. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pMSR:MSMEG_0310:Dendra

Plasmid

#159051

Purpose

T7 expression of C-term fluorescent fusion in E. coli

Depositor

Keith Derbyshire, Joseph Wade

Article

Judd et al mBio. 2021 Mar 2;12(2). pii: mBio.02401-

Insert

MSMEG_0310

Use

Integrating mycobacterial expression vector attl5

Tags

Gly-Ala Linker/Dendra2/1x Flag Tag

Expression

Bacterial

Promoter

pmycTetO

Available Since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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883 pET15b PTEN

Plasmid

#10936

Depositor

William Sellers

Insert

PTEN (PTEN Human)

Tags

His

Expression

Bacterial

Available Since

Jan. 3, 2006

Availability

Academic Institutions and Nonprofits only
dFP-mini

Plasmid

#120543

Purpose

Expresses dFP biliverdin-binding fluorescence-activating mini 10 KDa protein in bacteria.

Depositor

Brian Chow

Article

Sheehan et al Biochemistry. 2018 Nov 28. doi: 10.1021/

Insert

de novo fluorescence-activating protein mini (dFP-mini)

Tags

6xHis - TEV cleavage

Expression

Bacterial

Promoter

T7

Available Since

Jan. 30, 2019

Availability

Academic Institutions and Nonprofits only

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pEB1-sfGFP

Plasmid

#103983

Purpose

Plasmid encoding sfGFP

Depositor

Philippe Cluzel

Article

Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

Insert

sfGFP

Use

Low copy

Expression

Bacterial

Mutation

Mutations relative to wild-type GFP (S30R, Y39N, …

Promoter

proC

Available Since

Dec. 13, 2017

Availability

Academic Institutions and Nonprofits only

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p3521 pET ubcH6

Plasmid

#8653

Depositor

Peter Howley

Article

Scheffner et al Proc Natl Acad Sci U S A 1994 Sep 13;91(

Insert

UbcH6 (UBE2E1 Human)

Expression

Bacterial

Available Since

May 24, 2006

Availability

Academic Institutions and Nonprofits only
pKB230

Plasmid

#170017

Purpose

Encodes LacI-controlled expression of surface-displayed protegrin-1 and PvirK-mNeonGreen reporter

Depositor

Jeffrey Tabor

Article

Brink et al bioRxiv

Inserts

mNeonGreen
Protegrin-1

Use

Synthetic Biology

Tags

Lpp, OmpA, and Tether

Expression

Bacterial

Promoter

Ptac and PvirK

Available Since

Aug. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pGBW-m4046740

Plasmid

#145588

Purpose

Bacterial Expression plasmid for SARS-CoV-2 endoRNAse

Depositor

Ginkgo Bioworks, Benjie Chen

Insert

SARS-CoV-2 endoRNAse (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)

Tags

Cleavable TEV;6xHIS

Expression

Bacterial

Mutation

Escherichia coli recode 1

Promoter

Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

Available Since

April 29, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pBb(B5)8k-sfGFP

Plasmid

#110140

Purpose

IPTG-inducible pTRC promoter expressing sfGFP on BBR1-B5 origin

Depositor

Brian Pfleger

Article

Cook et al J Ind Microbiol Biotechnol. 2018 Jan 3.

Insert

sfGFP

Expression

Bacterial

Available Since

July 9, 2018

Availability

Academic Institutions and Nonprofits only

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