32,457 results
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Plasmid#100167PurposeUsed to improve production from engineered biosynthetic pathways include optimizing codon usage, enhancing production of rate-limiting enzymes, and eliminating the accumulation of toxic intermediates or byproducts to improve cell growthDepositorInsertMevT and MBIS operon
ExpressionBacterialPromoterlacUV5Available SinceSept. 15, 2017AvailabilityAcademic Institutions and Nonprofits only -
pVRb01_syn20
Plasmid#49704DepositorInsertsfgfp
UseSynthetic BiologyExpressionBacterialPromoterPecf01_syn20Available SinceJan. 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
pQIq_MRGS_HIS8_(27G2)_FLAG
Plasmid#199616PurposeEncodes DARPin-FLAG 27G2 for bacterial expression and column purificationDepositorInsertDARPin-FLAG 27G2
Tags8xHis and FLAGExpressionBacterialPromoterT5Available SinceMay 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET His6 prescission LIC cloning vector (HPKS)
Plasmid#29720DepositorTypeEmpty backboneTags6xHis and Prescission cleavage siteExpressionBacterialPromoterT7-lacO (lactose/IPTG inducible)Available SinceSept. 17, 2013AvailabilityAcademic Institutions and Nonprofits only -
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ETV4A-c072
Plasmid#53638Purposebacterial expression plasmidDepositorInsertETV4A
TagsHis-6-TEVExpressionBacterialMutationBacterial expression for structure determination;…PromoterT7Available SinceJune 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
p15aC-1D-5
Plasmid#107865PurposeBacterial expression of PIS and PI4P5KDepositorInsertsPIS
phosphatidylinositol 4-phosphate 5-kinase
TagsmycExpressionBacterialPromoterproD and proD (in operon)Available SinceApril 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
TPMT
Plasmid#25497PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceAug. 23, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits -
pH6HTC_NASP-Halo
Plasmid#175340PurposeProduction of HaloTag fusion proteinDepositorAvailable SinceOct. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSub-MBP-GFP(2M-IKQE)
Plasmid#185757PurposeBacterial expression of MBP-GFP(2M IKQE)DepositorInsertGFP(2M-IKQE)
TagsMBPExpressionBacterialPromotertacAvailable SinceJuly 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBbB1k-GFP
Plasmid#35342DepositorInsertGFP
UseSynthetic BiologyExpressionBacterialPromoterTrcAvailable SinceFeb. 20, 2012AvailabilityAcademic Institutions and Nonprofits only -
pET21TtTERT(195-516)
Plasmid#28172DepositorInsertTetrahymena TERT
ExpressionBacterialAvailable SinceFeb. 24, 2011AvailabilityAcademic Institutions and Nonprofits only -
pMOD3-mWasabi-nat
Plasmid#120331PurposeBacterial mWasabi promoter trapDepositorInsertmWasabi
ExpressionBacterialPromoter(None)Available SinceJune 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMS32: pTwinStrep-SUMO-PmcTnsC
Plasmid#168165PurposePmcTnsC expression construct for protein purification.DepositorInsertPmcCAST TnsC
Tags6X His, Twin Strep and SUMOExpressionBacterialAvailable SinceJune 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
MP6.6TAG_AraC_Para-dnaQ926-dam-seqA-emrR-ugi-CDA1.6TAG
Plasmid#163719PurposeArabinose-inducible expression of six mutagenesis genes, each encoding one amber codon after the initiator methionineDepositorInsertaraC dnaQ926.1TAG dam.1TAG seqA.1TAG emrR.1TAG ugi.1TAG CDA1.1TAG
ExpressionBacterialMutationdnaQ926.1TAG dam.1TAG seqA.1TAG emrR.1TAG ugi.1TA…Available SinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
B6-4
Plasmid#111488PurposePhe-tRNA synthetase ADepositorInsertPhe-tRNA synthetase A
Tags6xHisExpressionBacterialMutationA81SAvailable SinceSept. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMTB2-cytoBirA-2A-mCherry_Ras
Plasmid#80066PurposeBeta-actin2 promoter driving HA-tagged biotin ligase (BirA), a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequencesDepositorInsertBiotin ligase (BirA), a Thosea asigna virus peptide (2A), and mCherry protein
UseZebrafish biotaggingTags3x HAExpressionBacterialPromoterBeta-actin2Available SinceOct. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
sfTq2-PBP5
Plasmid#117959PurposePeriplasmic expression of superfolder mTurquoise2 (pNM067 pTHV037-DsbAss-LEGPAGL-sfTq2-EFGS-∆1-17-PBP5)DepositorInsertsfTq2-PBP5 (dacA )
ExpressionBacterialAvailable SinceJan. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJOE3075.3
Plasmid#135084PurposeL-rhamnose-inducible expression vectorDepositorInsertP-rhaP-BAD-malE
ExpressionBacterialAvailable SinceFeb. 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET-22b(+)-HpENR
Plasmid#12651DepositorInsertnoyl-acyl carrier protein reductase (fabI Helicobacter pylori)
ExpressionBacterialAvailable SinceJan. 5, 2007AvailabilityAcademic Institutions and Nonprofits only