31,868 results
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Plasmid#92060PurposeThe C-BLa 2.0 plasmid encodes a protein, where the TMD is connected to the C-terminus of the cytoplasmic domain of the ToxR protein and to the N-terminus of the C-terminal beta-lactamase fragment.DepositorInsertC-BLa 2.0 fusion protein
TagsFlag (DYKDHDG)ExpressionBacterialPromoteraraBADAvailable SinceJune 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSB_init_Sb92
Plasmid#159424PurposeExpression of the anti-TGP (thermostable GFP) synthetic nanobody Sb92DepositorInsertSb92
Tagsc-Myc and poly-HisExpressionBacterialAvailable SinceJan. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEDJ400
Plasmid#177269PurposeUSER cloning-compatible 2μ vector (URA3)DepositorTypeEmpty backboneExpressionBacterial and YeastAvailable SinceJan. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET His6 TEV co-transformation cloning vector (13S-B)
Plasmid#48324DepositorTypeEmpty backboneTagsHis6ExpressionBacterialAvailable SinceSept. 25, 2013AvailabilityAcademic Institutions and Nonprofits only -
bbCas9pluspAAA
Plasmid#82581PurposeThe plasmid encodes the T7 promoter, Cas9 (from pX330 #42230) and a stretch of poly-A. After linearization with restriction enzyme SapI It is used to produce in vitro transcribed mRNADepositorInsertCas9
ExpressionBacterialPromoterT7Available SinceNov. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pQLinkHD_EBFP2-LSSmOrange
Plasmid#118856Purposefluorescent protein expression with an N-terminal His-tagDepositorInsertEBFP2-LSSmOrange
TagsHis-tagExpressionBacterialMutationEBFP1.2-I128V/V150I/ D155V/V224R and for LSSmOra…Available SinceDec. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pANY6
Plasmid#112198PurposeThis plasmid is used for efficient cloning of DNA, efficient expression of protein in E. coli, and ELP-based protein purification.DepositorTypeEmpty backboneTagsELP-tagExpressionBacterialAvailable SinceAug. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
GALTA-c001
Plasmid#79645PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceJune 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTYB2-PAB1
Plasmid#37234DepositorAvailable SinceJuly 16, 2012AvailabilityAcademic Institutions and Nonprofits only -
pLITMUS-rpoN-cI5C6A,P-J23106-geneIII
Plasmid#80860PurposePhagemid (encodes evolved cI5C6A,P and M13 gene III)DepositorInsertsM13 gene III
cI5C6A,P
ExpressionBacterialPromoterBBa_J23106 and rpoNAvailable SinceDec. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET24a-LaM4-3xTS
Plasmid#162777PurposeBacterial expression of a functionalized anti-mCherry (LaM4) nanobody fused to three tyrosine sulfation (TS) motifs. LaM4-3xTS also contains a T7, HA, BAP and His6 epitopeDepositorInsertanti-mCherry nanobody fused to three TS sites, T7, HA, BAP and His6 epitope
TagsBAP, HA, His6, T7, and TS site from proCCKExpressionBacterialPromoterT7Available SinceOct. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDHK29
Plasmid#80783PurposeCloning vector compatible with ColE1 plasmids with high copy numberDepositorTypeEmpty backboneExpressionBacterialAvailable SinceNov. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGST-HsSyx-375-792
Plasmid#179292PurposeE. coli expression plasmid (T7 promoter) for expression-optimized DNA of human Syx (aa 375-792) with N-terminal His6 + GST + TEV protease cleavage siteDepositorInsertSyx
TagsHis6 + GST + TEV protease cleavage siteExpressionBacterialMutationresidues 375-792 from accession number NP_0010361…PromoterT7Available SinceJune 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
YWHAH
Plasmid#38814PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceMarch 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
pGEX p85a(C)-SH2
Plasmid#46463DepositorInsertp85a(C) (PIK3R1 Human)
TagsGST and PreScissionExpressionBacterialMutationcontains amino acids 612-724PromoterTacAvailable SinceJuly 23, 2013AvailabilityAcademic Institutions and Nonprofits only -
pBA789
Plasmid#186244Purposesoc-F HR Donor Vector (T4 phage editing)DepositorInsertBBR1 backbone + T4 phage homology
ExpressionBacterialAvailable SinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pF712-pET28a-Hs-delta44-GCDH-NHis wt
Plasmid#85113Purposeexpression of recombinant human mature (delta44) GCDH wt with N-terminal 6xHis-tagDepositorInserthuman mature (delta44) GCDH (GCDH Human)
Tags6xHisExpressionBacterialMutationsilent SNP: G1041TPromoterT7Available SinceNov. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
4MTL
Plasmid#60100PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertMETTL21C:PBC002-E06:C229066 (METTL21C Human)
Tags6xHisExpressionBacterialMutationSee commentsPromoterT7Available SinceOct. 22, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pMA7
Plasmid#79967PurposeTM-MAGE strainDepositorInsertsLambda Red recombinase beta subunit
DNA adenine methylase
UseSynthetic BiologyExpressionBacterialPromoterpBADAvailable SinceSept. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pVRa22_4450
Plasmid#49676DepositorInsertECF22_4450
UseSynthetic BiologyTagsHis6-PreScissionExpressionBacterialMutationCodon optimized for E.coliPromoterpT7-lacOAvailable SinceJan. 6, 2014AvailabilityAcademic Institutions and Nonprofits only