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pUDE731

Plasmid

#103008

Purpose

S. cerevisiae Episomal plasmid harboring Fncpf1 under the control of TDH3p

Depositor

Jean-Marc Daran

Article

Swiat et al Nucleic Acids Res. 2017 Dec 1;45(21):125

Insert

Fncpf1

Tags

3HA and NLS

Expression

Yeast

Mutation

human codon optimized

Promoter

TEF1

Available Since

Dec. 1, 2017

Availability

Academic Institutions and Nonprofits only

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p416GPD-PYK1

Plasmid

#34604

Depositor

Markus Ralser

Article

Gruning et al Cell Metab. 2011 Sep 7;14(3):415-27.

Insert

PYK1

Expression

Yeast

Promoter

GPD

Available Since

Jan. 11, 2012

Availability

Academic Institutions and Nonprofits only

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pFA6a-12Pk-LEU2MX6

Plasmid

#49179

Purpose

genomic 12Pk epitope tagging in S. pombe

Depositor

Eishi Noguchi

Article

Gadaleta et al Biotechniques. 2013 Nov;55(5):257-63. do

Type

Empty backbone

Tags

12Pk

Expression

Yeast

Available Since

Nov. 19, 2013

Availability

Academic Institutions and Nonprofits only

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pUCdeltaSS-26X

Plasmid

#108218

Purpose

Yeast reporter gene with glucocorticoid response element (GRE) upstream of CYC1 promoter driving lacZ expression

Depositor

Didier Picard, Keith Yamamoto

Article

Schena et al Science. 1988 Aug 19. 241(4868):965-7.

Insert

GRE-CYC1-lacZ

Expression

Yeast

Promoter

CYC1

Available Since

Sept. 15, 2018

Availability

Academic Institutions and Nonprofits only

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pV1539

Plasmid

#111434

Purpose

Genedrive entry vector for Candida albicans

Depositor

Gerald Fink

Article

Vyas et al mSphere. 2018 Apr 25;3(2). pii: 3/2/e001

Insert

CaCas9/sgRNA-BsmBI stuffer

Use

CRISPR

Expression

Yeast

Available Since

Aug. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pMZ288

Plasmid

#59897

Purpose

Combination adh1:cas9/rrk1:sgRNA for CRISPR genome editing in fission yeast: sgRNA targeted against ade6+

Depositor

Mikel Zaratiegui

Article

Jacobs et al Nat Commun. 2014 Oct 29;5:5344. doi: 10.

Inserts

Cas9
rrk1:sgRNA

Use

CRISPR

Expression

Yeast

Mutation

Silent muation of the CspCI site

Available Since

Oct. 29, 2014

Availability

Academic Institutions and Nonprofits only

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416Gal-SV40NLS-FUS-YFP

Plasmid

#29595

Depositor

Aaron Gitler

Article

Sun et al PLoS Biol. 2011 Apr;9(4):e1000614. doi:

Insert

NLS-FUS (FUS Human)

Tags

SV40 NLS and YFP

Expression

Yeast

Available Since

June 7, 2011

Availability

Academic Institutions and Nonprofits only
pPMVAd36

Plasmid

#98302

Purpose

Enhancing the lower-part mevalonate pathway; overexpressing yeast mevalonate pathway genes under the control of consitutive promoters or CUP1 promoter.

Depositor

Claudia Vickers

Article

Peng et al Biotechnol Biofuels. 2017 Feb 21;10:43.

Insert

PCUP1>ERG12>TNAT5-PTEF2>ERG8>TIDP1-TIDP1

Expression

Yeast

Available Since

Aug. 24, 2017

Availability

Academic Institutions and Nonprofits only

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pBBK05 pCAS-Tyr-[gRNA: BsaI GFP dropout] (NatR,AmpR)

Plasmid

#178989

Purpose

Sp.Cas9 and gRNA yeast expression vector for HDR-based gRNA introduction. Selection = Nourseothricin resistance

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

S. pyogenes Cas9

Use

CRISPR and Synthetic Biology

Expression

Yeast

Available Since

May 2, 2022

Availability

Academic Institutions and Nonprofits only

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pBBK67 pHomL-RPL18Bp-RPC19-Link-Scarlet(rfp)-SSA1t-HomR (AmpR)

Plasmid

#179051

Purpose

Vector containing a yeast integration cassette for RPL18B-driven expression of RPC19-Link-Scarlet(rfp), a marker for the nucleolus

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

RPL18Bp-RPC19-Link-Scarlet(rfp)

Expression

Yeast

Available Since

May 4, 2022

Availability

Academic Institutions and Nonprofits only

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CUP Cyc8 YFP p316

Plasmid

#15594

Depositor

Susan Lindquist

Article

Duennwald et al Proc Natl Acad Sci U S A. 2006 Jul 18. 1

Insert

CYC8 (CYC8 Budding Yeast)

Tags

YFP

Expression

Yeast

Available Since

Aug. 15, 2007

Availability

Academic Institutions and Nonprofits only
pDRF1-GW (n1)StayGold-T2A-mCherry

Plasmid

#205495

Purpose

Expression of (n1)StayGold-T2A-mCherry in yeast

Depositor

Bas Teusink

Article

Botman et al ACS Synth Biol. 2026; doi: https://doi.o

Insert

(n1)StayGold-T2A-mCherry

Expression

Yeast

Available Since

Sept. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pAG415GPD-ccdB-EGFP

Plasmid

#14194

Depositor

Susan Lindquist

Article

Alberti et al Yeast. 2007 Oct . 24(10):913-9.

Type

Empty backbone

Use

Gateway destination

Tags

EGFP

Expression

Yeast

Available Since

March 16, 2007

Availability

Academic Institutions and Nonprofits only

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pGRCRE

Plasmid

#129116

Purpose

Expresses cre recombinase in Z. rouxii yeast cells

Depositor

Paolo Giudici

Article

Bizzarri et al Yeast. 2019 Dec;36(12):711-722. doi: 10.

Insert

cre

Use

Cre/Lox

Expression

Yeast

Promoter

S. cerevisiae GAL1 promoter

Available Since

April 19, 2022

Availability

Academic Institutions and Nonprofits only

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pRS423-mitoT

Plasmid

#174525

Purpose

Expresses yeast variant of mitoT synthetic intermembrane tether bridging the inner and outer mitochondrial membranes

Depositor

Oleh Khalimonchuk

Article

Dietz et al Redox Biol. 2021 Oct;46:102125. doi: 10.

Insert

Yeast mitochondrial intermembrane tether

Use

Synthetic Biology

Tags

eGFP

Expression

Yeast

Promoter

MET25

Available Since

Jan. 3, 2022

Availability

Academic Institutions and Nonprofits only

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3322_pETcon_SARS2_Omicron-BA2

Plasmid

#184409

Purpose

yeast surface display of the SARS-CoV-2 Omicron BA.2 variant RBD

Depositor

Jesse Bloom

Article

Starr et al Science. 2022 Jun 28:eabo7896. doi: 10.1

Insert

SARS-CoV-2 Omicron BA.2 Spike receptor binding domain (RBD) (S SARS-CoV-2 virus)

Tags

HA and c-Myc

Expression

Yeast

Mutation

G339D+S371F+S373P+S375F+T376A+D405N+R408S+K417N+N…

Available Since

May 23, 2022

Availability

Academic Institutions and Nonprofits only

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FRP800_insul-(lexA-box)3-PminCYC1-Citrine-TCYC1

Plasmid

#58436

Purpose

Induces Citrine expression from a promoter containing 3 lexA boxes in yeast cells

Depositor

Joerg Stelling

Article

Ottoz et al Nucleic Acids Res. 2014 Jul 17. pii: gku

Insert

CitrineA206K

Expression

Yeast

Promoter

insul-(lexA-box)3-PminCYC1

Available Since

Aug. 27, 2014

Availability

Academic Institutions and Nonprofits only

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pJT82_GalL_nCDA1Δ198-BE3

Plasmid

#145046

Purpose

Expresses nCDA1Δ198-BE3 in yeast cells

Depositor

Ralph Bock

Article

Tan et al Nat Commun. 2019 Jan 25;10(1):439. doi:

Insert

nCDA1Δ198-BE3

Use

CRISPR

Expression

Yeast

Mutation

pmCDA1(1-198AA); spCas9(D10A)

Promoter

GalL

Available Since

July 10, 2020

Availability

Academic Institutions and Nonprofits only

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pDRF1 Cyterm-ytd8oxStayGold

Plasmid

#219853

Purpose

expression of Cyterm-ytd8oxStayGold in budding yeast

Depositor

Bas Teusink

Article

Botman et al ACS Synth Biol. 2026; doi: https://doi.o

Insert

Cyterm-ytd8oxStayGold

Expression

Yeast

Available Since

Aug. 27, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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LHP398 - Ub-K48R/K63R

Plasmid

#32167

Depositor

Linda Hicke

Insert

synthetic yeast Ubiquitin (UBA1, UBA2 Budding Yeast)

Expression

Yeast

Mutation

K48R and K63R

Promoter

CUP1

Available Since

Sept. 27, 2011

Availability

Academic Institutions and Nonprofits only