8,173 results
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Plasmid#133903PurposePositive control when used in combination with pAWH-largeT. Expression of Gal4BD-p53 hybrid protein. Homology regions for recombination with pAWHDepositorAvailable SinceDec. 20, 2019AvailabilityAcademic Institutions and Nonprofits only
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pROS12
Plasmid#107926Purposehph based Yeast/E.coli shuttle vector designed to clone and express Cas9 programming spacerDepositorInsertgRNA-CAN1.Y, gRNA-ADE2.Y
UseCRISPRExpressionYeastAvailable SinceApril 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-link-yoLSS-mKate2-CaURA3
Plasmid#44886PurposeYeast tagging vector to create gene fusion with yeast optimized LSS-mKate2 with CaUra3 selectionDepositorInsertyoLSS-mKate2
ExpressionYeastAvailable SinceOct. 16, 2013AvailabilityAcademic Institutions and Nonprofits only -
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pAG413GPD-EGFP-ccdB
Plasmid#14310DepositorTypeEmpty backboneUseGateway destinationTagsEGFPExpressionYeastAvailable SinceJune 18, 2007AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4046633
Plasmid#149161PurposeYeast Expression plasmid for SARS-CoV-2 ORF3a proteinDepositorInsertSARS-CoV-2 ORF3a protein (ORF3a Severe acute respiratory syndrome coronavirus 2, Budding Yeast)
ExpressionYeastMutationwtPromoterpTEF1Available SinceMay 14, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAV11K
Plasmid#63184PurposepRS416 backbone, URA3 replaced by KanMX, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG.DepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterial and YeastAvailable SinceSept. 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCHAP4
Plasmid#206850PurposeA plasmid containing a 16,121 bp fragment of the P. tricornutum chloroplast genome. The fragment is flanked by SapI recognition sites, allowing it to be released from the cloning vector.DepositorTypeEmpty backboneExpressionBacterial and YeastAvailable SinceFeb. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pNB783
Plasmid#60162PurposeFirefly luciferase (Promega) driven by RNR1 promoter.DepositorInsertFLuc
ExpressionYeastMutationA4V & S504G (no functional effect)PromoterRNR1prAvailable SinceMarch 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
pYC54
Plasmid#63909PurposeRecipient integrative vector to construct YFP tagged fusionsDepositorTypeEmpty backboneTagsYFPExpressionYeastAvailable SinceMay 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
pYC48
Plasmid#63905PurposeRecipient integrative vector to construct HA tagged fusionsDepositorTypeEmpty backboneTagsHAExpressionYeastAvailable SinceMay 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
YIPlac204-TC-Sec7-6xDsRed-M1
Plasmid#25448DepositorAvailable SinceJuly 14, 2010AvailabilityAcademic Institutions and Nonprofits only -
pHES820
Plasmid#89190PurposeExpresses YFP under control of GAL1 promoter.DepositorInsertpGAL1-YFP
ExpressionYeastPromoterGAL1Available SinceOct. 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
RPL3-GFP (96)
Plasmid#24040DepositorAvailable SinceMarch 31, 2010AvailabilityAcademic Institutions and Nonprofits only -
pDH3
Plasmid#83789PurposeUseful for tagging yeast proteins with C-terminal CFP tag; G418 selectionDepositorInsertCFP
TagsCFPExpressionYeastMutationF64L, Y66W, N146I, M153T, V163A, N164H mutations …Available SinceSept. 30, 2016AvailabilityAcademic Institutions and Nonprofits only -
YIplac211-APL2-iGFPx6
Plasmid#105251PurposePlasmid for pop-in/pop-out replacement of Apl2 with Apl2-iGFPx6.DepositorAvailable SinceJune 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJAS1052
Plasmid#158598PurposeVioA, VioBDepositorInsertsVioA
VioB
ExpressionYeastAvailable SinceOct. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
Linked Free NES
Plasmid#182485PurposeYeast integrative plasmid for expressing fusion protein ERG20-AcNES1, with linker sequence AG4TGGA (GAL10 promoter). Contains uracil auxotrophic marker (K. lactis URA3).DepositorInsertFPPS-NES
UseSynthetic Biology; Metabolic engineeringExpressionYeastPromoterGAL10Available SinceNov. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
Linked Free PcPTS
Plasmid#182486PurposeYeast integrative plasmid for expressing fusion protein ERG20-PcPTS, with linker sequence AG4TGGA (GAL10 promoter). Contains uracil auxotrophic marker (K. lactis URA3)DepositorInsertFPPS-PTS
UseSynthetic Biology; Metabolic engineeringExpressionYeastPromoterGAL10Available SinceNov. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCYC1pr-GNSI (LEU)
Plasmid#111450PurposeGNSI is a synthetic, genetically-encoded reporter that allows rapid plate-based assessment of AP-3 functional deficiency, using either colorimetric or growth phenotype readouts.DepositorInsertsCYC1 Promoter (CYC1 Budding Yeast)
Nyv1 Cytosolic Domain (aa 6-230)
Snc1 Transmembrane Domain (TMD)
SUC2 CDS
SUC2 terminator
TagsmGFP5ExpressionYeastMutationM109I (Naturally occurring SNP) and The first 21 …Available SinceJune 14, 2018AvailabilityAcademic Institutions and Nonprofits only