162,717 results

1. psPAX2

   Plasmid

   #12260

   Purpose

   2nd generation lentiviral packaging plasmid. Can be used with 2nd or 3rd generation lentiviral vectors and envelope expressing plasmid (Addgene#12259)

   Depositor

   Didier Trono

   Type

   Empty backbone

   Use

   Lentiviral; Packaging

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 4, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMD2.G

   Plasmid

   #12259

   Purpose

   VSV-G envelope expressing plasmid

   Depositor

   Didier Trono

   Insert

   VSV G
   

   Use

   Lentiviral; Envelope

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 4, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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3. lentiCRISPR v2

   Plasmid

   #52961

   Purpose

   Replaces original lentiCRISPRv1 (Addgene Plasmid 49535) and produces ~10-fold higher titer virus. 3rd generation lentiviral backbone.

   Depositor

   Feng Zhang

   Article

   Sanjana et al Nat Methods. 2014 Aug;11(8):783-4. doi:

   Inserts

   Cas9
   Puromycin resistance
   

   Use

   CRISPR and Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   EFS-NS

   Available since

   May 8, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSpCas9(BB)-2A-Puro (PX459) V2.0

   Plasmid

   #62988

   Purpose

   Cas9 from S. pyogenes with 2A-Puro, and cloning backbone for sgRNA (V2.0)

   Depositor

   Feng Zhang

   Article

   Ran et al Nat Protoc. 2013 Nov;8(11):2281-308. doi

   Has Service

   Cloning Grade DNA

   Insert

   hSpCas9-2A-Puro V2.0
   

   Use

   CRISPR

   Tags

   2A-Puro and 3XFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Cbh

   Available since

   March 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. lentiGuide-Puro

   Plasmid

   #52963

   Purpose

   Expresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and puromycin resistance from EF-1a promoter. 3rd generation lentiviral backbone.

   Depositor

   Feng Zhang

   Article

   Sanjana et al Nat Methods. 2014 Aug;11(8):783-4. doi:

   Has Service

   Cloning Grade DNA

   Inserts

   S. pyogenes sgRNA cassette
   Puromycin resistance
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Ef1-a and hU6

   Available since

   May 8, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. SP-dCas9-VPR

   Plasmid

   #63798

   Purpose

   SP-dCas9 with VP64-p65-Rta (VPR) fused to C-terminus; mammalian vector

   Depositor

   George Church

   Article

   Chavez et al Nat Methods. 2015 Mar 2. doi: 10.1038/nm

   Insert

   SP-dCas9-VPR
   

   Use

   CRISPR and Synthetic Biology

   Tags

   VPR (VP64-p65-Rta)

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 3, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAdDeltaF6

   Plasmid

   #112867

   Purpose

   AAV helper plasmid

   Depositor

   James M. Wilson

   Insert

   E4, E2a and VA
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. CRISPRoff-v2.1

   Plasmid

   #167981

   Purpose

   Expresses CRISPRoff-v2.1 (DNMT3A-DNMT3L-XTEN80-dCas9-HA-2xNLS-BFP-KRAB) downstream of the CAG promoter

   Depositor

   Luke Gilbert

   Article

   Nuñez et al Cell. 2021 Apr 7. pii: S0092-8674(21)003

   Insert

   CRISPRoff-v2.1 (DNMT3A-DNMT3L-dCas9-BFP-KRAB)
   

   Use

   Tags

   2xNLS, DNMT3A-DNMT3L, HA, KRAB, and tagBFP

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   May 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. lentiCas9-Blast

   Plasmid

   #52962

   Purpose

   Expresses human codon-optimized S. pyogenes Cas9 protein and blasticidin resistance from EFS promoter. 3rd generation lentiviral backbone.

   Depositor

   Feng Zhang

   Article

   Sanjana et al Nat Methods. 2014 Aug;11(8):783-4. doi:

   Has Service

   Lentiviral Prep

   Inserts

   Cas9
   Blasticidin resistance
   

   Use

   CRISPR and Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   EFS-NS

   Available since

   May 8, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. pEF1a-hMLH1dn

    Plasmid

    #174824

    Purpose

    Mammalian expression of human MLH1dn (codon optimized)

    Depositor

    David Liu

    Article

    Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.

    Insert

    human MLH1dn (codon optimized)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    deletion of residues 754-756

    Promoter

    EF1a

    Available since

    Oct. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pU6-tevopreq1-GG-acceptor

    Plasmid

    #174038

    Purpose

    Prime editing in mammalian cells

    Depositor

    David Liu

    Article

    Nelson et al Nat Biotechnol. 2021 Oct 4. pii: 10.1038

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    Oct. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3/F-tractin=mStayGold

    Plasmid

    #212019

    Purpose

    For filamentous actin labeling. Alternatively, the F-tractin gene can be replaced with a target molecule gene for C-terminal tagging with mStayGold through a linker [(GGGGS)3] (denoted as ‘=’).

    Depositor

    Atsushi Miyawaki

    Article

    Ando et al Nat Methods. 2023 Nov 30. doi: 10.1038/s

    Insert

    F-tractin
    

    Use

    Tags

    mStayGold

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV-MCS2

    Plasmid

    #46954

    Purpose

    Cloning plasmid for the generation of Knock-In in human cells using rAAV.

    Depositor

    Steve Jackson

    Article

    Britton et al J Cell Biol. 2013 Jul 29.

    Type

    Empty backbone

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 19, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCBASceI

    Plasmid

    #26477

    Purpose

    I-SceI endonuclease expression vector with mammalian promoter to introduce a DSB at a genomic I-SceI site

    Depositor

    Maria Jasin

    Article

    Richardson et al Genes Dev. 1998 Dec 15. 12(24):3831-42.

    Insert

    pCBASceI
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 20, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLKO.1 puro

    Plasmid

    #8453

    Purpose

    3rd gen lentiviral backbone for cloning and expression of new shRNA sequences. Uses puromycin for selection.

    Depositor

    Bob Weinberg

    Article

    Stewart et al RNA 2003 Apr;9(4):493-501.

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 18, 2005

    Availability

    Academic Institutions and Nonprofits only

    

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16. eeBxb1

    Plasmid

    #222339

    Purpose

    Plasmid for expressing evolved and engineered Bxb1 in the mammalian cells

    Depositor

    David Liu

    Article

    Pandey et al Nat Biomed Eng. 2024 Jun 10. doi: 10.103

    Insert

    Codon-optimized evolved and engineered Bxb1
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. Tet-pLKO-puro

    Plasmid

    #21915

    Purpose

    3rd generation lentiviral plasmid for inducible expression of shRNA; puromycin selection. See manual for detailed protocols. No further technical support is available from the depositing laboratory.

    Depositor

    Dmitri Wiederschain

    Article

    Wiederschain et al Cell Cycle. 2009 Feb 1. 8(3):498-504.

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 22, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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18. pTW1169

    Plasmid

    #236583

    Purpose

    TRV1

    Depositor

    Steven Jacobsen

    Article

    Weiss et al Nat Plants. 2025 Apr 22. doi: 10.1038/s4

    Insert

    TRV1
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    May 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. SB100X in pCAG globin pA

    Plasmid

    #127909

    Purpose

    Sleeping Beauty Transposase expression vector driven by the CAG promoter

    Depositor

    Mark Groudine

    Article

    Strom et al Elife. 2021 Jun 9;10. pii: 63972. doi: 1

    Insert

    Sleeping Beauty Transposase
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLVX-TetOne-Puro-GFP

    Plasmid

    #171123

    Purpose

    Doxycycline inducible expression of GFP

    Depositor

    Jason Sheltzer

    Article

    Lukow et al Dev Cell. 2021 Sep 13;56(17):2427-2439.e

    Insert

    EGFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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