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Showing: 1 - 20 of 159111 results

  1. psPAX2

    Plasmid
    #12260
    Purpose
    2nd generation lentiviral packaging plasmid. Can be used with 2nd or 3rd generation lentiviral vectors and envelope expressing plasmid (Addgene#12259)
    Depositor
    Didier Trono
    Type
    Empty backbone
    Use
    Lentiviral; Packaging
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 4, 2006
    Availability
    Academic Institutions and Nonprofits only
    12260-plasmid-map-sequence-id-467229

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  2. pMD2.G

    Plasmid
    #12259
    Purpose
    VSV-G envelope expressing plasmid
    Depositor
    Didier Trono
    Insert
    VSV G
    Use
    Lentiviral; Envelope
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 4, 2006
    Availability
    Academic Institutions and Nonprofits only
    12259-plasmid-map-sequence-id-443804

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  3. lentiCRISPR v2

    Plasmid
    #52961
    Purpose
    Replaces original lentiCRISPRv1 (Addgene Plasmid 49535) and produces ~10-fold higher titer virus. 3rd generation lentiviral backbone.
    Depositor
    Feng Zhang
    Article
    Sanjana et al Nat Methods. 2014 Aug;11(8):783-4. doi:
    Inserts
    Cas9
    Puromycin resistance
    Use
    CRISPR and Lentiviral
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    EFS-NS
    Available since
    May 8, 2014
    Availability
    Academic Institutions and Nonprofits only
    52961-plasmid-map-sequence-id-244694

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  4. pAdDeltaF6

    Plasmid
    #112867
    Purpose
    AAV helper plasmid
    Depositor
    James M. Wilson
    Insert
    E4, E2a and VA
    Use
    AAV
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 22, 2018
    Availability
    Academic Institutions and Nonprofits only
    112867-plasmid-map-sequence-id-372973

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  11. pMDLg/pRRE

    Plasmid
    #12251
    Purpose
    3rd generation lentiviral packaging plasmid; Contains Gag and Pol; also requires pRSV-Rev (Addgene#12253) and envelope expressing plasmid (Addgene#12259)
    Depositor
    Didier Trono
    Article
    Dull et al J Virol. 1998 Nov . 72(11):8463-71.
    Insert
    HIV-1 GAG/POL
    Use
    Lentiviral; Packaging
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 4, 2006
    Availability
    Academic Institutions and Nonprofits only
    12251-plasmid-map-sequence-id-433915

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  12. pRSV-Rev

    Plasmid
    #12253
    Purpose
    3rd generation lentiviral packaging plasmid; Contains Rev; also requires pMDLg/pRRE (Addgene#12251) and envelope expressing plasmid (Addgene#12259)
    Depositor
    Didier Trono
    Article
    Dull et al J Virol. 1998 Nov . 72(11):8463-71.
    Insert
    Rev (rev )
    Use
    Lentiviral; Packaging
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 4, 2006
    Availability
    Academic Institutions and Nonprofits only
    12253-plasmid-map-sequence-id-443803

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  13. lentiGuide-Puro

    Plasmid
    #52963
    Purpose
    Expresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and puromycin resistance from EF-1a promoter. 3rd generation lentiviral backbone.
    Depositor
    Feng Zhang
    Article
    Sanjana et al Nat Methods. 2014 Aug;11(8):783-4. doi:
    Has Service
    Cloning Grade DNA
    Inserts
    S. pyogenes sgRNA cassette
    Puromycin resistance
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Ef1-a and hU6
    Available since
    May 8, 2014
    Availability
    Academic Institutions and Nonprofits only
    52963-plasmid-map-sequence-id-331247

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  15. pCMV-VSV-G

    Plasmid
    #8454
    Purpose
    Envelope protein for producing lentiviral and MuLV retroviral particles. Use in conjunction with a packaging vector such as pCMV-dR8.2 dvpr (lentiviral) or pUMVC (MuLV retroviral).
    Depositor
    Bob Weinberg
    Article
    Stewart et al RNA 2003 Apr;9(4):493-501.
    Type
    Empty backbone
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    June 20, 2005
    Availability
    Academic Institutions and Nonprofits only
    8454-plasmid-map-sequence-id-404044

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  19. PB-TO-hNGN2

    Plasmid
    #172115
    Purpose
    Piggybac Tet-ON plasmid for differentiating hiPSCs into glutamatergic neurons via NGN2 expression
    Depositor
    Michael Ward, iPSC Neurodegenerative Disease Initiative (iNDI)
    Article
    Pantazis et al Cell Stem Cell. 2022 Dec 1;29(12):1685-1
    Inserts
    hNGN2 (NEUROG2 Human)
    puroR-T2A-mycNLS-mTagBFP2
    Use
    Tags
    T2A-mycNLS-mTagBFP2
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a and TRE3G
    Available since
    April 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    172115-plasmid-map-sequence-id-448148

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  20. pAAV2/8

    Plasmid
    #112864
    Purpose
    AAV packaging plasmid expressing Rep/Cap genes
    Depositor
    James M. Wilson
    Insert
    Rep2/Cap8
    Use
    AAV
    Tags
    Expression
    Mutation
    Promoter
    Truncated P5
    Available since
    Aug. 28, 2018
    Availability
    Academic Institutions and Nonprofits only
    112864-plasmid-map-sequence-id-333531

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Showing: 1 - 20 of 159111 results