8,086 results

1. pHES854

   Plasmid

   #89197

   Purpose

   Expresses mKate2 under control of TDH3 constitutive promoter.

   Depositor

   Hana El-Samad

   Article

   Aranda-Diaz et al ACS Synth Biol. 2016 Dec 27. doi: 10.102

   Insert

   pTDH3-mKate2
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   TDH3

   Available Since

   Dec. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pRS416-PHO5-GFP-Osh3

   Plasmid

   #58839

   Purpose

   Expresses GFP-Tagged Osh3 in yeast

   Depositor

   Sean Munro, Tim Levine

   Article

   Levine et al Mol Biol Cell. 2001 Jun;12(6):1633-44.

   Insert

   Osh3p (OSH3 Budding Yeast)
   

   Use

   Tags

   GFP and Myc

   Expression

   Yeast

   Mutation

   Promoter

   PHO5

   Available Since

   Jan. 20, 2015

   Availability

   Academic Institutions and Nonprofits only

3. 1000.pCCLsin.cPPT.hPGK.Myt1L.MIRT124_x4 Xma_lost.WPRE

   Plasmid

   #67293

   Purpose

   Induce human iN conversion from fibroblast

   Depositor

   Malin Parmar

   Article

   Lau et al Cell Rep. 2014 Dec 11;9(5):1673-80. doi:

   Insert

   Myt1L (Myt1l Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian and Yeast

   Mutation

   Promoter

   PGK

   Available Since

   Aug. 20, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCfB2804

   Plasmid

   #63644

   Purpose

   pCfB2804 is a vector for multiple integrations at sites sharing homology with Ty2Cons. The selective marker is Kl.LEU2. Additionally it contains a USER cassette.

   Depositor

   Jerome Maury, Irina Borodina

   Article

   Maury et al PLoS One. 2016 Mar 2;11(3):e0150394. doi

   Type

   Empty backbone

   Use

   Cre/Lox

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   none

   Available Since

   Aug. 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. C-SWAT GFP CL1

   Plasmid

   #182512

   Purpose

   C-SWAT compatible vector for C-terminal tagging with GFP followed by the CL1 degron for constitutive gene expression knockdown.

   Depositor

   Rob Mitra

   Article

   Shively et al ACS Synth Biol. 2023 Jan 17. doi: 10.102

   Insert

   CL1 Degron
   

   Use

   Tags

   GFP

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   Jan. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. YIplac211-VPS8-iYFPx6

   Plasmid

   #105272

   Purpose

   Plasmid for pop-in/pop-out replacement of Vps8 with Vps8-iYFPx6.

   Depositor

   Benjamin Glick

   Article

   Day et al Dev Cell. 2018 Jan 8;44(1):56-72.e4. doi

   Insert

   Vps8 portion (VPS8 Budding Yeast)
   

   Use

   Tags

   iYFPx6

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   June 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGAD424-hSyntenin2b

   Plasmid

   #89427

   Purpose

   Two-hybrid system

   Depositor

   Fritz Rathjen

   Article

   Koroll et al J Biol Chem. 2001 Apr 6;276(14):10646-54

   Insert

   SDCBP2 (SDCBP2 Human)
   

   Use

   Tags

   Gal4 AD

   Expression

   Yeast

   Mutation

   *see below

   Promoter

   ADH1

   Available Since

   July 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. p416PGK1-OsCYP711A2(pYL770)

   Plasmid

   #178301

   Purpose

   Expresses OsCYP711A2 from O. sativa in Saccharomyces cerevisiae. Centromeric URA, PPGK1-OsCYP711A2-Tpho5

   Depositor

   Yanran Li

   Article

   Wu et al Sci Adv. 2021 Sep 17;7(38):eabh4048. doi

   Insert

   OsCYP711A2
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   PGK1

   Available Since

   Sept. 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pAG413GPD-ccdB-ECFP

   Plasmid

   #14166

   Depositor

   Susan Lindquist

   Article

   Alberti et al Yeast. 2007 Oct . 24(10):913-9.

   Type

   Empty backbone

   Use

   Gateway destination

   Tags

   ECFP

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   March 15, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pFA6a-PATagRFP-KanMX6

    Plasmid

    #87027

    Purpose

    Tag S. pombe genes in C terminal with PATagRFP

    Depositor

    Julien Berro

    Insert

    PATagRFP
    

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available Since

    Feb. 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pDS296

    Plasmid

    #34985

    Depositor

    Michael Glotzer

    Article

    Strickland et al Nat Methods. 2012 Mar 4. doi: 10.1038/nm

    Insert

    ePDZb1
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    GAL1

    Available Since

    March 23, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCT-L7.5.1

    Plasmid

    #42900

    Depositor

    Dane Wittrup

    Article

    Hackel et al J Mol Biol. 2008 Sep 19;381(5):1238-52.

    Insert

    L7.5.1
    

    Use

    Tags

    HA and c-myc

    Expression

    Yeast

    Mutation

    Promoter

    Gal1-10

    Available Since

    June 26, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGM-ENO2-YFP-mCherry

    Plasmid

    #180569

    Purpose

    Dual fluorescent reporter for 5' UTR activity in yeast YFP/mCherry

    Depositor

    Joel McManus

    Article

    May et al Elife. 2023 May 25;12:e69611. doi: 10.75

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    March 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pDN-G1TGt

    Plasmid

    #44508

    Depositor

    Gabor Balazsi

    Article

    Nevozhay et al Proc Natl Acad Sci U S A. 2009 Mar 31;10

    Inserts

    PGal1-D12 promoter (GAL1 Budding Yeast, Synthetic)
    tetR::yEGFP
    

    Use

    Synthetic Biology; Expression regulator/reporter

    Tags

    Expression

    Yeast

    Mutation

    Two tandem tet operators (2XtetO2) downstream of …

    Promoter

    PGal1-D12 promoter

    Available Since

    April 23, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMC6-TEFkFLP(LYS2)

    Plasmid

    #49458

    Purpose

    overespression of Flp step-arrest mutant in yeast, LYS2 selection

    Depositor

    Marc Gartenberg

    Insert

    Flp(H305L) (flp Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    H305L, ‘step-arrest’ mutant,

    Promoter

    TEF1

    Available Since

    Dec. 3, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGBW-m4046299

    Plasmid

    #153878

    Purpose

    Yeast expression plasmid for SARS-CoV-2 S (surface glycoprotein)

    Depositor

    Benjie Chen, Ginkgo Bioworks

    Insert

    SARS-CoV-2 S (surface glycoprotein) (S Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Cleavable thrombin;6xHIS

    Expression

    Yeast

    Mutation

    wt

    Promoter

    pGAL1

    Available Since

    June 12, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

17. LDB107

    Plasmid

    #185423

    Purpose

    NUP1-HA CEN TRP1 AmpR. Yeast shuttle plasmid for expression of hemagluttinin tagged NUP1 nucleoporin

    Depositor

    Ken Belanger, Laura Davis

    Article

    Belanger et al J Cell Biol. 1994 Aug;126(3):619-30. doi

    Insert

    NUP1
    

    Use

    Tags

    HA

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    July 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pAG306GPD-EGFP-ccdB

    Plasmid

    #14308

    Depositor

    Susan Lindquist

    Article

    Alberti et al Yeast. 2007 Oct . 24(10):913-9.

    Type

    Empty backbone

    Use

    Gateway destination

    Tags

    EGFP

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    June 18, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pPSG830

    Plasmid

    #158601

    Purpose

    tNCS-ePTS1 (2micron)

    Depositor

    John Dueber

    Article

    Grewal et al Nat Chem Biol. 2020 Oct 12. pii: 10.1038

    Insert

    pTDH3-tNCS-ePTS1
    

    Use

    Tags

    ePTS1

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Oct. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAG414GPD-ccdB-HA

    Plasmid

    #14240

    Depositor

    Susan Lindquist

    Article

    Alberti et al Yeast. 2007 Oct . 24(10):913-9.

    Type

    Empty backbone

    Use

    Gateway destination

    Tags

    3HA

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    March 29, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge