8,223 results

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YIplac204-TC-OM45-FKBPx2

Plasmid

#104471

Purpose

Integrating plasmid for overexpression of OM45-FKBPx2.

Depositor

Benjamin Glick

Article

Papanikou et al Elife. 2015 Dec 28;4. pii: e13232. doi:

Insert

OM45p (OM45 Budding Yeast)

Tags

FKBPx2- HA

Expression

Yeast

Promoter

TPI1

Available Since

Feb. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pBJ1808 pRS416-COF1RFP

Plasmid

#37103

Depositor

John Cooper

Article

Lin et al J Cell Sci. 2010 Apr 15;123(Pt 8):1329-4

Insert

COF1-RFP

Tags

RFP

Expression

Yeast

Mutation

Single RFP internal insertion at Cof147T48D with …

Available Since

June 19, 2012

Availability

Academic Institutions and Nonprofits only
pAG414GAL-ccdB-EGFP

Plasmid

#14191

Depositor

Susan Lindquist

Article

Alberti et al Yeast. 2007 Oct . 24(10):913-9.

Type

Empty backbone

Use

Gateway destination

Tags

EGFP

Expression

Yeast

Available Since

March 15, 2007

Availability

Academic Institutions and Nonprofits only

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pFA6a-kanMX-tetO-Pcyc1-3xFLAG-LINKER

Plasmid

#52689

Purpose

Same as 41022, with additional linker

Depositor

Michael Nick Boddy

Insert

tetO7-Pcyc1-3xFLAG+LINKER

Use

Promoter replacement

Expression

Yeast

Available Since

April 23, 2014

Availability

Academic Institutions and Nonprofits only

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LD211

Plasmid

#69183

Purpose

Integration of 2GFP tag at Atg11 C terminus, use auxotrophic marker URA3(Candida albicans)

Depositor

Zhiping Xie

Article

Li et al Autophagy. 2015;11(6):954-60. doi: 10.10

Insert

Atg11-2EGFP

Tags

2xEGFP

Expression

Yeast

Available Since

Oct. 28, 2015

Availability

Academic Institutions and Nonprofits only

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pAG424GPD-ccdB-HA

Plasmid

#14248

Depositor

Susan Lindquist

Article

Alberti et al Yeast. 2007 Oct . 24(10):913-9.

Type

Empty backbone

Use

Gateway destination

Tags

3HA

Expression

Yeast

Available Since

March 29, 2007

Availability

Academic Institutions and Nonprofits only

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pH3FS-PYK1

Plasmid

#85781

Purpose

N-ICE plasmid pH3FS-PYK1

Depositor

Scott Briggs

Article

Zhang et al Yeast. 2016 Dec 12. doi: 10.1002/yea.322

Insert

loxP-HphMX-loxP-PYK1p-3FLAG

Use

Cre/Lox

Expression

Yeast

Available Since

May 19, 2017

Availability

Academic Institutions and Nonprofits only

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pV1464

Plasmid

#111440

Purpose

N. castellii Solo CRISPR vector, marked with URA3 and NatR

Depositor

Gerald Fink

Article

Vyas et al mSphere. 2018 Apr 25;3(2). pii: 3/2/e001

Insert

CaCas9/sgRNA-BsmBI stuffer

Use

CRISPR

Expression

Yeast

Available Since

Aug. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pDH18

Plasmid

#83803

Purpose

YFP-CFP positive FRET control; his selection

Depositor

Eric Muller

Article

Hailey et al Methods Enzymol. 2002;351:34-49.

Insert

YFP-CFP

Expression

Yeast

Available Since

Oct. 28, 2016

Availability

Academic Institutions and Nonprofits only

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pDA200

Plasmid

#74248

Purpose

pGPD1-dPSTRy(4,3)

Depositor

Serge Pelet

Article

Aymoz et al Nat Commun. 2016 Apr 21;7:11304. doi: 10

Insert

pGPD1-dPSTRy(4,3)

Expression

Bacterial and Yeast

Available Since

May 4, 2016

Availability

Academic Institutions and Nonprofits only

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pCfB2226

Plasmid

#67533

Purpose

EasyClone system-based yeast integrative vector carrying loxP-flanked SpHIS5 marker, integration into S. cerevisiae X-4 chromosomal location, USER site for cloning, amp resistance

Depositor

Irina Borodina

Article

Stovicek et al J Ind Microbiol Biotechnol. 2015 Sep 16.

Type

Empty backbone

Use

Cre/Lox; Integrative

Expression

Yeast

Available Since

March 10, 2016

Availability

Academic Institutions and Nonprofits only

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pPICZc-Ub-R-HsACTB*-thymosinB-8His

Plasmid

#111149

Purpose

Expresses arginylated human beta-actin fused with thymosin beta and a His tag.

Depositor

Mohan Balasubramanian

Article

Hatano et al J Cell Sci. 2018 Mar 13. pii: jcs.213827

Insert

ACTB (ACTB Human)

Use

Pichia pastoris integration plasmid

Tags

Thymosin beta and a His-tag

Expression

Yeast

Mutation

N-terminal arginylation

Promoter

AOX1

Available Since

June 27, 2018

Availability

Academic Institutions and Nonprofits only

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NAT-TET-OFF -MET - 3xHA

Plasmid

#182526

Purpose

Doxycycline-induced transcriptional regulation and 3xHA N-terminal tagging.

Depositor

Rob Mitra

Article

Shively et al ACS Synth Biol. 2023 Jan 17. doi: 10.102

Insert

7 Copies of TetO Box

Tags

3HA

Expression

Yeast

Available Since

Jan. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pTY24

Plasmid

#83794

Purpose

Useful for tagging yeast proteins with C-terminal DsRed tag; G418 selection

Depositor

Eric Muller

Article

Hailey et al Methods Enzymol. 2002;351:34-49.

Insert

DsRed

Tags

DsRed

Expression

Yeast

Available Since

Sept. 30, 2016

Availability

Academic Institutions and Nonprofits only

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YIplac211-iGFP-TLG2

Plasmid

#105262

Purpose

Plasmid for pop-in/pop-out replacement of Tlg2 with iGFP-Tlg2.

Depositor

Benjamin Glick

Article

Day et al Dev Cell. 2018 Jan 8;44(1):56-72.e4. doi

Insert

Tlg2 (TLG2 Budding Yeast)

Tags

iGFP

Expression

Yeast

Available Since

Jan. 25, 2018

Availability

Academic Institutions and Nonprofits only

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pJK59-ATG18-GFP

Plasmid

#42529

Depositor

James Hurley

Article

Baskaran et al Mol Cell. 2012 Aug 10;47(3):339-48. doi:

Insert

Atg18 (ATG18 Budding Yeast)

Tags

GFP

Expression

Yeast

Mutation

none

Available Since

July 29, 2013

Availability

Academic Institutions and Nonprofits only
pJZC595

Plasmid

#62322

Purpose

Expresses MCP-VP64 and dCas9 in Yeast cells

Depositor

Wendell Lim, Stanley Qi

Article

Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

Inserts

MCP-VP64
dCas9

Tags

VP64

Expression

Yeast

Mutation

Has nuclease-inactivating mutations in the RuvC1 …

Promoter

Tdh3 and pAdh

Available Since

March 23, 2015

Availability

Academic Institutions and Nonprofits only

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pXP414

Plasmid

#26841

Depositor

Nancy DaSilva, Suzanne Sandmeyer

Article

Fang et al Yeast. 2011 Feb;28(2):123-36. doi: 10.10

Type

Empty backbone

Expression

Yeast

Available Since

Dec. 8, 2010

Availability

Academic Institutions and Nonprofits only

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YIplac211-ARF1-msGFP

Plasmid

#105255

Purpose

Plasmid for pop-in/pop-out replacement of Arf1 with Arf1-msGFP.

Depositor

Benjamin Glick

Article

Day et al Dev Cell. 2018 Jan 8;44(1):56-72.e4. doi

Insert

Arf1 (ARF1 Budding Yeast)

Tags

msGFP

Expression

Yeast

Available Since

Jan. 25, 2018

Availability

Academic Institutions and Nonprofits only

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pAG413GPD-ccdB-HA

Plasmid

#14238

Depositor

Susan Lindquist

Article

Alberti et al Yeast. 2007 Oct . 24(10):913-9.

Type

Empty backbone

Use

Gateway destination

Tags

3HA

Expression

Yeast

Available Since

March 29, 2007

Availability

Academic Institutions and Nonprofits only

Enlarge