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pCT-L7.5.1

Plasmid

#42900

Depositor

Dane Wittrup

Article

Hackel et al J Mol Biol. 2008 Sep 19;381(5):1238-52.

Insert

L7.5.1

Tags

HA and c-myc

Expression

Yeast

Promoter

Gal1-10

Available Since

June 26, 2013

Availability

Academic Institutions and Nonprofits only

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LD118

Plasmid

#69170

Purpose

Integration of GFP tag at Atg18 C terminus, use auxotrophic marker URA3(Candida albicans)

Depositor

Zhiping Xie

Article

Li et al Autophagy. 2015;11(6):954-60. doi: 10.10

Insert

Atg18-EGFP

Tags

EGFP

Expression

Yeast

Available Since

Sept. 16, 2015

Availability

Academic Institutions and Nonprofits only

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pAG424GAL-ccdB-HA

Plasmid

#14247

Depositor

Susan Lindquist

Article

Alberti et al Yeast. 2007 Oct . 24(10):913-9.

Type

Empty backbone

Use

Gateway destination

Tags

3HA

Expression

Yeast

Available Since

March 29, 2007

Availability

Academic Institutions and Nonprofits only

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pGBW-m4046310

Plasmid

#149022

Purpose

Yeast Expression plasmid for SARS-CoV-2 M (membrane)

Depositor

Ginkgo Bioworks, Benjie Chen

Insert

SARS-CoV-2 M (membrane) (M Severe acute respiratory syndrome coronavirus 2, Budding Yeast)

Tags

Cleavable thrombin;MBP

Expression

Yeast

Mutation

wt

Promoter

pTEF1

Available Since

May 14, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pGBW-m4046423

Plasmid

#148998

Purpose

Yeast Expression plasmid for SARS-CoV-2 nsp7

Depositor

Ginkgo Bioworks, Benjie Chen

Insert

SARS-CoV-2 nsp7 (ORF1ab Severe acute respiratory syndrome coronavirus 2, Budding Yeast)

Tags

Cleavable thrombin;MBP

Expression

Yeast

Mutation

wt

Promoter

pTEF1

Available Since

May 14, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pRS415-Cas9-VPR

Plasmid

#163971

Purpose

Trifunctional Cas9-VPR fusion protein plasmid for simultaneous transcriptional activation, transcriptional repression, and genome editing in yeast

Depositor

Jiazhang Lian

Article

Dong et al ACS Synth Biol. 2020 Sep 18;9(9):2252-22

Insert

Cas9-VPR

Use

Synthetic Biology

Expression

Yeast

Available Since

Feb. 2, 2021

Availability

Academic Institutions and Nonprofits only

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p41Nat MagicFluor

Plasmid

#58563

Purpose

p41Nat carrying both mating type markers buffered by the Cyc1 terminator

Depositor

Leonid Kruglyak

Insert

Ste2p-Citrine, Cyc1 term, Ste3p-mCherry

Expression

Yeast

Available Since

Nov. 24, 2014

Availability

Academic Institutions and Nonprofits only

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pGP8GF-AtTIR1

Plasmid

#188427

Purpose

Arabidopsis Nuclear Auxin Signaling Receptor TIR1

Depositor

Britney Moss

Article

Ramos Baez et al Plant Physiol. 2020 Apr;182(4):1713-1722

Insert

Auxin Signaling Receptor TIR1 (TIR1 Mustard Weed)

Expression

Yeast

Available Since

Aug. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pCfB2804

Plasmid

#63644

Purpose

pCfB2804 is a vector for multiple integrations at sites sharing homology with Ty2Cons. The selective marker is Kl.LEU2. Additionally it contains a USER cassette.

Depositor

Irina Borodina, Jerome Maury

Article

Maury et al PLoS One. 2016 Mar 2;11(3):e0150394. doi

Type

Empty backbone

Use

Cre/Lox

Expression

Yeast

Promoter

none

Available Since

Aug. 15, 2016

Availability

Academic Institutions and Nonprofits only

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pAG414GPD-GaCYP722C(pYL777)

Plasmid

#178290

Purpose

Expresses GaCYP722C from G. arboreum in Saccharomyces cerevisiae. Centromeric TRP, PGPD-GaCYP722C-TCYC1

Depositor

Yanran Li

Article

Wu et al Sci Adv. 2021 Sep 17;7(38):eabh4048. doi

Insert

GaCYP722C

Expression

Yeast

Promoter

GAP

Available Since

Sept. 6, 2022

Availability

Academic Institutions and Nonprofits only

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pBS-SKII-URA-GAL1-HALO-GS

Plasmid

#188940

Purpose

Integration cassette to create N-terminal fusions for fluorescent tagging

Depositor

Stephen Buratowski

Article

Baek et al G3 (Bethesda). 2022 Aug 9. pii: 6659097.

Insert

pBS-SKII-URA-GAL1-HALO-GS

Expression

Yeast

Available Since

Sept. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pAV0612

Plasmid

#133505

Purpose

Expression of 3xmTagBFP2 targeted to the plasma membrane with an amphipathic helix of RitC

Depositor

Sophie Martin

Article

Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

Insert

pAde6(PmeI)-p(tdh1)-3mTagBFP2-RitC-terminator(ScADH1)-bsdMX

Expression

Yeast

Available Since

Nov. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pHES873

Plasmid

#87945

Purpose

Expresses estradiol-inducible synthetic transcription factor in yeast under control of GAL1 promoter. It activates transcription of promoters containing Zif268 binding sites.

Depositor

Hana El-Samad

Article

Aranda-Diaz et al ACS Synth Biol. 2016 Dec 27. doi: 10.102

Insert

pGAL1- Zif268 DBD - hPR LBD - MSN2 AD (PGR Human, Budding Yeast)

Expression

Yeast

Promoter

GAL1

Available Since

Dec. 1, 2017

Availability

Academic Institutions and Nonprofits only

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pCP5

Plasmid

#35397

Depositor

Daniel Voytas

Article

Cermak et al Nucleic Acids Res. 2011 Jul;39(12):e82.

Type

Empty backbone

Use

Reporter

Expression

Yeast

Promoter

GDP promoter

Available Since

Aug. 24, 2012

Availability

Academic Institutions and Nonprofits only

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pPTK001-2-pAOX1

Plasmid

#84960

Purpose

Type 2, pAOX1, Alcohol oxidase 1 promoter

Depositor

Volker Sieber

Article

Obst et al ACS Synth Biol. 2017 Mar 15. doi: 10.102

Insert

Alcohol oxidase 1 promoter

Use

Synthetic Biology

Expression

Yeast

Promoter

Non

Available Since

April 3, 2017

Availability

Academic Institutions and Nonprofits only

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pFA6a-zeoCV

Plasmid

#73890

Purpose

fission yeast drug selection marker

Depositor

Kazunori Tomita

Article

Amelina et al BMC Biotechnol. 2016 Nov 8;16(1):76.

Insert

zeoCV

Expression

Bacterial, Mammalian, and Y…

Promoter

CMV

Available Since

Nov. 17, 2016

Availability

Academic Institutions and Nonprofits only

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pDS271

Plasmid

#34974

Depositor

Michael Glotzer

Article

Strickland et al Nat Methods. 2012 Mar 4. doi: 10.1038/nm

Insert

Mid2-GFP-LOVpep (MID2 A. sativa, Budding Yeast)

Expression

Yeast

Mutation

T406A, T407A, V416I

Promoter

TEF

Available Since

March 23, 2012

Availability

Academic Institutions and Nonprofits only

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pILGFPQ4

Plasmid

#83559

Purpose

Used to evaluate the expression output of GAL7 promoter with yEGFP used as the reporter gene.

Depositor

Claudia Vickers

Article

Peng et al Biotechnol Biofuels. 2017 Feb 21;10:43.

Insert

GAL7 promoter-yEGFP

Expression

Yeast

Promoter

GAL7

Available Since

Dec. 15, 2016

Availability

Academic Institutions and Nonprofits only

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YIplac211-VPS8-iYFPx6

Plasmid

#105272

Purpose

Plasmid for pop-in/pop-out replacement of Vps8 with Vps8-iYFPx6.

Depositor

Benjamin Glick

Article

Day et al Dev Cell. 2018 Jan 8;44(1):56-72.e4. doi

Insert

Vps8 portion (VPS8 Budding Yeast)

Tags

iYFPx6

Expression

Yeast

Available Since

June 8, 2018

Availability

Academic Institutions and Nonprofits only

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pTPI1-EMC1-GFP-APEX2-URA3

Plasmid

#184776

Purpose

Expresses EMC1-GFP-APEX2 chimera for labeling yeast endoplasmic reticulum (ER). Uses auxotrophic marker URA3(Kluyveromyces lactis).

Depositor

Zhiping Xie

Article

Li et al BMC Biol. 2020 Aug 28;18(1):107. doi: 10

Insert

APEX2

Expression

Yeast

Available Since

Jan. 19, 2023

Availability

Academic Institutions and Nonprofits only

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