8,388 results
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Plasmid#212929PurposeEncodes the mRuby2 fluorescent protein (Part3b') with the 111AA SCW4 TFP (Part3a') assembled with S. cerevisiae pTEF1 promoter, tTDH1 terminator, LEU2 selectable marker and CEN/ARS yeast origin.DepositorInsertmRuby2
ExpressionYeastAvailable SinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pYSD141
Plasmid#212928PurposeEncodes the mRuby2 fluorescent protein (Part3b') with the Aga2 signal peptide (Part3a') assembled with S. cerevisiae pTEF1 promoter, tTDH1 terminator, LEU2 selectable marker and CEN/ARS yeast origin.DepositorInsertmRuby2
ExpressionYeastAvailable SinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEK19
Plasmid#210578PurposeReporter gene expression in B. dendrobatidisDepositorInsertBdBIP1pro-Hshph-G4S-nanoluc-ScADH1ter
UseLuciferaseTagsNanoLuc luciferaseExpressionBacterial and YeastPromoterBdBIP1proAvailable SinceJan. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEK22
Plasmid#210588PurposeReporter gene expression in B. dendrobatidisDepositorInsertBdH3pro-Hshph-G4S-nanoluc-ScADH1ter
UseLuciferaseTagsNanoLuc luciferaseExpressionBacterial and YeastPromoterBdH3proAvailable SinceJan. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHflu4
Plasmid#203882PurposepHI1 with insertion of monomeric red fluorescent protein (mRFP)DepositorInsertmonomeric red fluorescent protein
UseSynthetic BiologyExpressionBacterial and YeastAvailable SinceDec. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDRF1-GW (n1)StayGold-T2A-mCherry
Plasmid#205495PurposeExpression of (n1)StayGold-T2A-mCherry in yeastDepositorInsert(n1)StayGold-T2A-mCherry
ExpressionYeastAvailable SinceSept. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJS288
Plasmid#200714PurposeTo express yeast Hxt1 hexose transporter in yeastDepositorUseSynthetic BiologyTagsmCherryExpressionYeastMutationdomesticated for MoClo cloning (removal of intern…Available SinceAug. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMJ273: pRS426-His-MBP-GtFz1_PSP1
Plasmid#205258PurposeS. cerevisiae expression vector for GtFz1 reprogrammed guide (targeting PSP1)DepositorInsertGtFz1
Tags10xHis, MBPExpressionYeastAvailable SinceAug. 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAG416GAL-EMCV_3C
Plasmid#201941PurposeExpresses EMCV 3C protease from a GAL promoter with a URA3 markerDepositorAvailable SinceAug. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRS305-LEU2-pHIS3-SV40NLS-PfV-GS-GFPMUT3
Plasmid#203657PurposeYeast integrative vector for the expression of the GFPMUT3-tagged nuclear localizing 40nm nucGEMs under the HIS3 promoter.DepositorArticleInsertPfV
TagsGFPMUT3ExpressionYeastAvailable SinceJuly 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGBT9-Hook3
Plasmid#198525PurposeExpression of GAL4 DNA-binding domain (BD)-Hook3 fusion protein in yeast (yeast two-hybrid assays)DepositorInsertHook3 (HOOK3 Human)
TagsGAL4-DNA binding domain (BD) fragmentExpressionYeastPromoterADH1Available SinceApril 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDSM-bc-Ptdh3-CAD-Ttdh1
Plasmid#127727PurposeYeast pathway position 3. CAD transcription unit with the TDH3 promoter and TDH1 terminator.DepositorInsertcis-aconitate decarboxylase
UseSynthetic BiologyExpressionYeastPromoterPtdh3Available SinceMarch 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
UG75-PAH1-3HA
Plasmid#184761PurposePAH1 overexpression. Promotes the formation of diacylglycerol from phosphatidate. Uses antibiotic resistance marker hphMX6.DepositorAvailable SinceFeb. 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pICE HIS3
Plasmid#122877PurposeHIS3 ICE plasmid used to facilitate yeast recombination cloning and stable genomic Integration after CEN Excision.DepositorTypeEmpty backboneUseYeast recombination cloningExpressionYeastAvailable SinceFeb. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
C-SWAT AID*-GFP osTIR1
Plasmid#182514PurposeC-SWAT compatible vector for C-terminal tagging with AID*-GFP for auxin inducible gene knockdown. osTIR1 is flanked by SIR4 homology arms for integration.DepositorInsertAID*
TagsGFPExpressionYeastAvailable SinceJan. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTR1-GPD-TRPC
Plasmid#190103PurposeExpression vector for fungal (Aspergillus flavus) genes based on pyrithiamine resistance (ptrA) selectionDepositorTypeEmpty backboneExpressionYeastAvailable SinceJan. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGAL-SKI1-NAT
Plasmid#160382PurposeOverxpression of SKI1/XRN1 in yeastDepositorInsertSKI1
ExpressionYeastPromoterGALAvailable SinceDec. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRS306-PTDH3-SUMO10-6SIM
Plasmid#190248PurposeExpression SUMO10-SIM6 in yeast cellsDepositorInsert10SUMO-6SIM
ExpressionYeastAvailable SinceNov. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
Linked Free ClLIS1 (M)
Plasmid#182487PurposeYeast integrative plasmid for expressing fusion protein ERG20(F96W-N127W)-ClLIS1, with linker sequence AG4TGGA (GAL10 promoter). Contains uracil auxotrophic marker (K. lactis URA3)DepositorInsertFPPS(M)-LS
UseSynthetic Biology; Metabolic engineeringExpressionYeastPromoterGAL10Available SinceNov. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pgRNA-backbone
Plasmid#172517PurposeYeast plasmid encoding a 2-kb gRNA-placeholder flanked by BsmBI/Esp3I restriction sites to facilitate gRNA insertionDepositorInsertgRNA cassette
UseCRISPRExpressionYeastPromoterSNR52Available SinceNov. 7, 2022AvailabilityAcademic Institutions and Nonprofits only