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p4A8_S7T_BC

Plasmid

#217832

Purpose

4A8 variant in barcoded, yeast surface display plasmid

Depositor

Timothy Whitehead

Publication

Petersen et al Nat Commun. 2024 May 10;15(1):3974. doi:

Insert

4A8 variant Fab

Expression

Yeast

Mutation

VL: S7T

Available since

April 24, 2024

Availability

Academic Institutions and Nonprofits only

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pYSD_kappa_mRFP

Plasmid

#217830

Purpose

Yeast surface display Fab vector with kappa light chain and mRFP

Depositor

Timothy Whitehead

Publication

Petersen et al Nat Commun. 2024 May 10;15(1):3974. doi:

Type

Empty backbone

Expression

Yeast

Available since

April 22, 2024

Availability

Academic Institutions and Nonprofits only

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ClhN-pADH3-Tgl3-4V5-APEX2-URA

Plasmid

#207066

Purpose

Expressing Tgl3-4V5-APEX2. Marker for lipid droplets.

Depositor

Zhiping Xie

Publication

Li et al mSphere. 2022 Aug 31;7(4):e0010722. doi:

Insert

Tgl3

Tags

4xV5-APEX2

Expression

Yeast

Promoter

pADH3

Available since

March 4, 2024

Availability

Academic Institutions and Nonprofits only

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pYSD300

Plasmid

#212930

Purpose

Encodes the S. cerevisiae Aga1 protein (Part3) with pGBK1 promoter, tENO2 terminator, HIS3 selectable marker and CEN/ARS yeast origin.

Depositor

Paul Young

Publication

O'Riordan et al ACS Synth Biol. 2024 Mar 14. doi: 10.102

Insert

AGA1 (AGA1 Budding Yeast)

Expression

Yeast

Available since

Feb. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pYSD153

Plasmid

#212929

Purpose

Encodes the mRuby2 fluorescent protein (Part3b') with the 111AA SCW4 TFP (Part3a') assembled with S. cerevisiae pTEF1 promoter, tTDH1 terminator, LEU2 selectable marker and CEN/ARS yeast origin.

Depositor

Paul Young

Publication

O'Riordan et al ACS Synth Biol. 2024 Mar 14. doi: 10.102

Insert

mRuby2

Expression

Yeast

Available since

Feb. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pYSD141

Plasmid

#212928

Purpose

Encodes the mRuby2 fluorescent protein (Part3b') with the Aga2 signal peptide (Part3a') assembled with S. cerevisiae pTEF1 promoter, tTDH1 terminator, LEU2 selectable marker and CEN/ARS yeast origin.

Depositor

Paul Young

Publication

O'Riordan et al ACS Synth Biol. 2024 Mar 14. doi: 10.102

Insert

mRuby2

Expression

Yeast

Available since

Feb. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pEK19

Plasmid

#210578

Purpose

Reporter gene expression in B. dendrobatidis

Depositor

Lillian Fritz-Laylin

Publication

Kalinka et al Proc Natl Acad Sci U S A. 2024 Jan 23;12

Insert

BdBIP1pro-Hshph-G4S-nanoluc-ScADH1ter

Use

Luciferase

Tags

NanoLuc luciferase

Expression

Bacterial and Yeast

Promoter

BdBIP1pro

Available since

Jan. 22, 2024

Availability

Academic Institutions and Nonprofits only

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pEK22

Plasmid

#210588

Purpose

Reporter gene expression in B. dendrobatidis

Depositor

Lillian Fritz-Laylin

Publication

Kalinka et al Proc Natl Acad Sci U S A. 2024 Jan 23;12

Insert

BdH3pro-Hshph-G4S-nanoluc-ScADH1ter

Use

Luciferase

Tags

NanoLuc luciferase

Expression

Bacterial and Yeast

Promoter

BdH3pro

Available since

Jan. 22, 2024

Availability

Academic Institutions and Nonprofits only

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pDN0606 (pDEST-DHFR F[3] N-term,HygR)

Plasmid

#210488

Purpose

pDEST vector to tag gene of interest with DHFR F[3] on the N-terminus to perform DHFR-PCA in S. cerevisiae.

Depositor

Christian Landry

Publication

Evans-Yamamoto et al Mol Biol Evol. 2023 Nov 18:msad246. doi:

Type

Empty backbone

Use

Synthetic Biology; Destination vector for gateway…

Expression

Yeast

Promoter

ADH1

Available since

Dec. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pKB11 (pDEST-DHFR F[1,2] C-term, NatR)

Plasmid

#210485

Purpose

pDEST vector to tag gene of interest with DHFR F[1,2] on the C-terminus to perform DHFR-PCA in S. cerevisiae.

Depositor

Christian Landry

Publication

Evans-Yamamoto et al Mol Biol Evol. 2023 Nov 18:msad246. doi:

Type

Empty backbone

Use

Synthetic Biology; Destination vector for gateway…

Expression

Yeast

Promoter

ADH1

Available since

Dec. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pHflu4

Plasmid

#203882

Purpose

pHI1 with insertion of monomeric red fluorescent protein (mRFP)

Depositor

Bogumil Karas

Insert

monomeric red fluorescent protein

Use

Synthetic Biology

Expression

Bacterial and Yeast

Available since

Dec. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pDN0605 (pDEST-DHFR F[1,2] N-term, NatR)

Plasmid

#210487

Purpose

pDEST vector to tag gene of interest with DHFR F[1,2] on the N-terminus to perform DHFR-PCA in S. cerevisiae.

Depositor

Christian Landry

Publication

Evans-Yamamoto et al Mol Biol Evol. 2023 Nov 18:msad246. doi:

Type

Empty backbone

Use

Synthetic Biology; Destination vector for gateway…

Expression

Yeast

Promoter

ADH1

Available since

Dec. 4, 2023

Availability

Academic Institutions and Nonprofits only

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pKB12 (pDEST-DHFR F[3] C-term, HygR)

Plasmid

#210486

Purpose

pDEST vector to tag gene of interest with DHFR F[3] on the C-terminus to perform DHFR-PCA in S. cerevisiae.

Depositor

Christian Landry

Publication

Evans-Yamamoto et al Mol Biol Evol. 2023 Nov 18:msad246. doi:

Type

Empty backbone

Use

Synthetic Biology; Destination vector for gateway…

Expression

Yeast

Promoter

ADH1

Available since

Nov. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pDRF1-GW (n1)StayGold-T2A-mCherry

Plasmid

#205495

Purpose

Expression of (n1)StayGold-T2A-mCherry in yeast

Depositor

Bas Teusink

Publication

Botman et al ACS Synth Biol. 2026 Apr 1. doi: 10.1021

Insert

(n1)StayGold-T2A-mCherry

Expression

Yeast

Available since

Sept. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pJS288

Plasmid

#200714

Purpose

To express yeast Hxt1 hexose transporter in yeast

Depositor

Jens Boch

Publication

Fuhrmeister et al Bio Protoc. 2023 Aug 5;13(15):e4733. doi

Inserts

PMA1 promoter (PMA1 Budding Yeast)
Hxt1 (HXT1 Budding Yeast)
ADH2 Ter (ADH2 Budding Yeast)

Use

Synthetic Biology

Tags

mCherry

Expression

Yeast

Mutation

domesticated for MoClo cloning (removal of intern…

Available since

Aug. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pMJ273: pRS426-His-MBP-GtFz1_PSP1

Plasmid

#205258

Purpose

S. cerevisiae expression vector for GtFz1 reprogrammed guide (targeting PSP1)

Depositor

Feng Zhang

Publication

Saito et al Nature. 2023 Jun 28. doi: 10.1038/s41586

Insert

GtFz1

Tags

10xHis, MBP

Expression

Yeast

Available since

Aug. 15, 2023

Availability

Academic Institutions and Nonprofits only

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pAG416GAL-EMCV_3C

Plasmid

#201941

Purpose

Expresses EMCV 3C protease from a GAL promoter with a URA3 marker

Depositor

Alejandro Chavez

Publication

Hong et al Mol Syst Biol. 2025 Feb;21(2):158-172. d

Insert

EMCV 3C protease (EMCVgp1 )

Expression

Yeast

Promoter

GAL1

Available since

Aug. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pRS305-LEU2-pHIS3-SV40NLS-PfV-GS-GFPMUT3

Plasmid

#203657

Purpose

Yeast integrative vector for the expression of the GFPMUT3-tagged nuclear localizing 40nm nucGEMs under the HIS3 promoter.

Depositor

Liam Holt

Publication

Shu T et et al bioRxiv 2022

Insert

PfV

Tags

GFPMUT3

Expression

Yeast

Available since

July 25, 2023

Availability

Academic Institutions and Nonprofits only

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pGBT9-Hook3

Plasmid

#198525

Purpose

Expression of GAL4 DNA-binding domain (BD)-Hook3 fusion protein in yeast (yeast two-hybrid assays)

Depositor

Juan Bonifacino

Publication

Mattera et al Mol Biol Cell. 2020 Apr 15;31(9):963-979

Insert

Hook3 (HOOK3 Human)

Tags

GAL4-DNA binding domain (BD) fragment

Expression

Yeast

Promoter

ADH1

Available since

April 3, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pDSM-bc-Ptdh3-CAD-Ttdh1

Plasmid

#127727

Purpose

Yeast pathway position 3. CAD transcription unit with the TDH3 promoter and TDH1 terminator.

Depositor

Christopher Voigt

Publication

Young et al Metab Eng. 2018 Jul;48:33-43. doi: 10.10

Insert

cis-aconitate decarboxylase

Use

Synthetic Biology

Expression

Yeast

Promoter

Ptdh3

Available since

March 1, 2023

Availability

Academic Institutions and Nonprofits only

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