8,086 results

1. pFA6a-TKnatAX

   Plasmid

   #73895

   Purpose

   fission yeast drug counter-selectable selection marker

   Depositor

   Kazunori Tomita

   Article

   Amelina et al BMC Biotechnol. 2016 Nov 8;16(1):76.

   Insert

   TK-nat
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   Nov. 17, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pFA6a-link-mKO1-CaUra3

   Plasmid

   #44638

   Purpose

   Yeast tagging vector to create gene fusion with mKO1 with CaUra3 selection

   Depositor

   Kurt Thorn

   Insert

   mKO1
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   May 7, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pYES2.1-cerMET3

   Plasmid

   #107436

   Purpose

   Overexpression of MET fusion protein in S. cerevisiae

   Depositor

   Clare O'Connor

   Article

   Reeves et al CBE Life Sci Educ. 2018 Spring;17(1). pi

   Insert

   MET3 (MET3 Budding Yeast)
   

   Use

   Tags

   V5-His

   Expression

   Yeast

   Mutation

   Promoter

   GAL1

   Available Since

   April 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pYES2.1-cerMET17

   Plasmid

   #107443

   Purpose

   Overexpression of MET fusion protein in S. cerevisiae

   Depositor

   Clare O'Connor

   Article

   Reeves et al CBE Life Sci Educ. 2018 Spring;17(1). pi

   Insert

   MET17 (MET17 Budding Yeast)
   

   Use

   Tags

   V5-His

   Expression

   Yeast

   Mutation

   Promoter

   GAL1

   Available Since

   April 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. THI VP64 pGAP T5

   Plasmid

   #161492

   Purpose

   CRISPR/RNA scaffold based transcription regulation in Pichia pastoris BB3rN_pTEF2_dCAS9(3xFLAG_SV40NLS)_pPOR1_MS2bind_VP64_SV40NLS_pGAP_THI11_T5_gRNA_MS2

   Depositor

   Diethard Mattanovich, Brigitte Gasser

   Article

   Baumschabl et al ACS Synth Biol. 2020 Dec 18;9(12):3202-3

   Inserts

   dCAS9
   MS2-VP64
   gRNA (Targeting T5 in the THI11 promoter)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   pGAP, pPOR1, and pTEF2

   Available Since

   March 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pUB23-L-beta-gal

   Plasmid

   #8820

   Depositor

   Alexander Varshavsky

   Article

   Bachmair et al Science 1986 Oct 10;234(4773):179-86.

   Insert

   beta-gal
   

   Use

   Tags

   Ubiquitin

   Expression

   Yeast

   Mutation

   L (leucine) at amino terminus of beta-gal

   Promoter

   Available Since

   Dec. 28, 2005

   Availability

   Academic Institutions and Nonprofits only

7. THI VP64 pGAP T1

   Plasmid

   #161488

   Purpose

   CRISPR/RNA scaffold based transcription regulation in Pichia pastoris BB3rN_pTEF2_dCAS9(3xFLAG_SV40NLS)_pPOR1_MS2bind_VP64_SV40NLS_pGAP_THI11_T1_gRNA_MS2

   Depositor

   Diethard Mattanovich, Brigitte Gasser

   Article

   Baumschabl et al ACS Synth Biol. 2020 Dec 18;9(12):3202-3

   Inserts

   dCAS9
   MS2-VP64
   gRNA (Targeting T1 in the THI11 promoter)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   pGAP, pPOR1, and pTEF2

   Available Since

   March 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pGBW-m4046574

   Plasmid

   #148976

   Purpose

   Yeast Expression plasmid for SARS-CoV-2 surface glycoprotein (Spike protein)

   Depositor

   Benjie Chen, Ginkgo Bioworks

   Insert

   SARS-CoV-2 S (surface glycoprotein) (S Severe acute respiratory syndrome coronavirus 2, Budding Yeast)
   

   Use

   Tags

   Cleavable thrombin;3xFLAG

   Expression

   Yeast

   Mutation

   Saccharomyces cerevisiae recode 1

   Promoter

   pGAL1

   Available Since

   May 14, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. pBES2

   Plasmid

   #83800

   Purpose

   Useful for tagging yeast proteins with N-terminal Venus tag; G418 selection

   Depositor

   Eric Muller

   Article

   Hailey et al Methods Enzymol. 2002;351:34-49.

   Insert

   Venus
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   Sept. 30, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. p42Neo 3-F GW

    Plasmid

    #58558

    Purpose

    Multicopy with G418 resistance marker for triple Gateway insertion

    Depositor

    Leonid Kruglyak

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Sept. 26, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pFA6a-link-yoPS-CFP2-SpHis5

    Plasmid

    #44853

    Purpose

    Yeast tagging vector to create gene fusion with yeast optimized photoconvertible CFP2 with SpHis5 selection

    Depositor

    Kurt Thorn, Wendell Lim

    Article

    Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

    Insert

    yoPS-CFP2
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Oct. 16, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pYES2.1-cerMET14

    Plasmid

    #107441

    Purpose

    Overexpression of MET fusion protein in S. cerevisiae

    Depositor

    Clare O'Connor

    Article

    Reeves et al CBE Life Sci Educ. 2018 Spring;17(1). pi

    Insert

    MET14 (MET14 Budding Yeast)
    

    Use

    Tags

    V5-His

    Expression

    Yeast

    Mutation

    Promoter

    GAL1

    Available Since

    April 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. E2-Crimson-FKBP(L)

    Plasmid

    #85001

    Purpose

    Expresses E2-Crimson-FKBP(L)

    Depositor

    Benjamin Glick

    Article

    Barrero et al Cell Logist. 2016 Jun 24;6(3):e1204848.

    Insert

    E2-Crimson-FKBP(L)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Dec. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. YIp211-P30-His-POL30-3'

    Plasmid

    #99546

    Purpose

    Yeast integrative vector for expression of His-tagged POL30 (PCNA); URA3 marker

    Depositor

    Helle Ulrich

    Article

    Davies et al Methods Mol Biol. 2012;920:543-67. doi:

    Insert

    POL30 (POL30 Budding Yeast)
    

    Use

    Tags

    6xHis

    Expression

    Yeast

    Mutation

    Promoter

    POL30

    Available Since

    Sept. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pFS485

    Plasmid

    #89348

    Purpose

    Leu1 integrating plasmid with cdc25 genomic fragment

    Depositor

    Nick Rhind

    Article

    Keifenheim et al Curr Biol. 2017 May 22;27(10):1491-1497.

    Insert

    cdc25 (cdc25 Fission Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    July 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCSN074

    Plasmid

    #166731

    Purpose

    Single copy yeast plasmid expressing DNase-dead dLbCas12a E925A fused to NLS, codon optimized for Saccharomyces cerevisiae

    Depositor

    Rene Verwaal

    Article

    Ciurkot et al Nucleic Acids Res. 2021 Jul 1. pii: 6312

    Insert

    dCas12a-NLS
    

    Use

    CRISPR

    Tags

    SV40 NLS

    Expression

    Yeast

    Mutation

    E925A

    Promoter

    S. cerevisiae TEF1

    Available Since

    June 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pDRf1-SweetTrac1

    Plasmid

    #169083

    Purpose

    Expression of SweetTrac1 biosensor in yeast

    Depositor

    Lily S Cheung

    Article

    Park et al Proc Natl Acad Sci U S A. 2022 Jan 25;11

    Insert

    SweetTrac1
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    PMA1

    Available Since

    Feb. 4, 2022

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

18. pHUM

    Plasmid

    #64170

    Purpose

    harbours marker genes HIS3, URA3 and MET17

    Depositor

    Markus Ralser

    Article

    Mulleder et al F1000Res. 2016 Sep 20;5:2351. eCollectio

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available Since

    Sept. 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pXP732

    Plasmid

    #46058

    Depositor

    Nancy DaSilva

    Article

    Shen et al Yeast. 2012 Dec;29(12):495-503. doi: 10.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    CUP1

    Available Since

    Sept. 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pYS104

    Plasmid

    #1190

    Depositor

    Susan Lindquist

    Article

    Patino et al Science 1996 Aug 2;273(5275):622-6.

    Insert

    Hsp104 (HSP104 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    wild-type Hsp104 sequence with 588bp of 5' p…

    Promoter

    Available Since

    June 23, 2005

    Availability

    Academic Institutions and Nonprofits only