8,133 results

1. deltaVP1 + VP2C-GFP

   Plasmid

   #166676

   Purpose

   Yeast integrative plasmid for expressing Murine polyomavirus deltaVP1 (GAL1 promoter) and GFP tagged with VP2C (GAL10 promoter). Contains uracil auxotrophic marker (K. lactis URA3)

   Depositor

   Claudia Vickers

   Article

   Cheah et al ACS Synth Biol. 2021 Dec 17;10(12):3251-

   Inserts

   VP2C-GFP
   Murine polyomavirus deltaVP1 (NLS deletion mutant)
   

   Use

   Synthetic Biology

   Expression

   Yeast

   Promoter

   GAL1 and GAL10

   Available Since

   April 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. LD116

   Plasmid

   #69168

   Purpose

   Integration of GFP tag at Atg16 C terminus, use auxotrophic marker URA3(Candida albicans)

   Depositor

   Zhiping Xie

   Article

   Li et al Autophagy. 2015;11(6):954-60. doi: 10.10

   Insert

   Atg16-GFP
   

   Tags

   EGFP

   Expression

   Yeast

   Available Since

   Nov. 3, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pWZ414-F53

   Plasmid

   #23077

   Depositor

   Sharon Dent

   Article

   Zhang et al EMBO J. 1998 Jun 1. 17(11):3155-67.

   Insert

   yeast Histone H3-2 and Histone H4-2
   

   Expression

   Yeast

   Mutation

   Histone H3 changed Lysine 9 to Arginine, H3 K9R

   Available Since

   April 12, 2010

   Availability

   Academic Institutions and Nonprofits only

4. pWZ414-F30

   Plasmid

   #23066

   Depositor

   Sharon Dent

   Article

   Zhang et al EMBO J. 1998 Jun 1. 17(11):3155-67.

   Insert

   yeast Histone H3-2 and Histone H4-2
   

   Expression

   Yeast

   Mutation

   Histone H3 changed lysine 9 to Glutamine H3 K9Q

   Available Since

   Feb. 5, 2010

   Availability

   Academic Institutions and Nonprofits only

5. pPS1888

   Plasmid

   #1360

   Depositor

   Pamela Silver

   Article

   Damelin et al Mol Cell 2000 Jan;5(1):133-40.

   Insert

   NLS-EYFP
   

   Expression

   Yeast

   Available Since

   Aug. 3, 2005

   Availability

   Academic Institutions and Nonprofits only

6. S3_002_026_096

   Plasmid

   #134403

   Purpose

   pYTK096-pRPL18B_NLS_FdeR-N_tPGK1-pGPM1-fdeO_mcherry_tTDH1

   Depositor

   Mattheos Koffas

   Article

   Wang et al ACS Synth Biol. 2019 Sep 20;8(9):2121-21

   Inserts

   mCherry
   NLS_FdeR-N
   

   Tags

   None and none

   Expression

   Yeast

   Mutation

   None and none

   Promoter

   pGPM1 and pRPL18B

   Available Since

   Dec. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pHES833

   Plasmid

   #90409

   Purpose

   Expresses YFP under control of GAL1 promoter

   Depositor

   Hana El-Samad

   Article

   Aranda-Diaz et al ACS Synth Biol. 2016 Dec 27. doi: 10.102

   Insert

   pZ-YFP
   

   Use

   Synthetic Biology

   Expression

   Yeast

   Available Since

   Nov. 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCfB5219

   Plasmid

   #106135

   Purpose

   EasyCloneYALI system-based yeast integrative vector carrying loxP-flanked hygromycin-resistance marker, integration into Yarrowia lipolytica chromosomal location IntE_4, USER site for cloning, amp resistance

   Depositor

   Irina Borodina

   Article

   Holkenbrink et al Biotechnol J. 2018 Jan 29. doi: 10.1002/

   Type

   Empty backbone

   Expression

   Yeast

   Available Since

   April 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. PP75

   Plasmid

   #78945

   Purpose

   Supplemental Figure 3. Integration Site 2.

   Depositor

   Timothy Lu

   Article

   Perez-Pinera et al Nat Commun. 2016 Jul 29;7:12211. doi: 10

   Insert

   Integration Site 2
   

   Expression

   Yeast

   Available Since

   Feb. 9, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGBW-m4046620

    Plasmid

    #153894

    Purpose

    Yeast expression plasmid for SARS-CoV-2 M (membrane)

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 M (membrane) (M Severe acute respiratory syndrome coronavirus 2)
    

    Expression

    Yeast

    Mutation

    wt

    Promoter

    pGAL1

    Available Since

    June 12, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. pBBK31 pHomL-TDH3p-Scarlet(rfp)-Link-GFPdropout-SSA1t-HomR (AmpR)

    Plasmid

    #179015

    Purpose

    Parent vector for markerless yeast integration cassettes with N-terminal Scarlet(rfp)-tagged genes

    Depositor

    Vincent Martin

    Article

    Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

    Type

    Empty backbone

    Expression

    Yeast

    Available Since

    May 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGBW-m4046581

    Plasmid

    #149064

    Purpose

    Yeast Expression plasmid for SARS-CoV-2 RNA-dependent RNA polymerase

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 RNA-dependent RNA polymerase (ORF1ab Severe acute respiratory syndrome coronavirus 2, Budding Yeast)
    

    Tags

    Cleavable thrombin;6xHIS

    Expression

    Yeast

    Mutation

    Saccharomyces cerevisiae recode 1

    Promoter

    pGAL1

    Available Since

    May 14, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. pLyGo-Kp-2

    Plasmid

    #163144

    Purpose

    pLyGo cloning vector for a sequence of interest (LPMO) in K. phaffii. Vector encoding the alfa-amylase signal peptide and the LyGo cassette (SapI-ccdB-SapI)

    Depositor

    Morten Norholm

    Article

    Hernandez-Rollan et al ACS Synth Biol. 2021 Apr 16;10(4):897-90

    Type

    Empty backbone

    Use

    Synthetic Biology

    Expression

    Yeast

    Promoter

    AOX1

    Available Since

    May 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGBW-m4046445

    Plasmid

    #145493

    Purpose

    Yeast Expression plasmid for SARS-CoV-2 nsp9

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 nsp9 (ORF1ab Severe acute respiratory syndrome coronavirus 2, Budding Yeast)
    

    Tags

    Cleavable thrombin;6xHIS

    Expression

    Yeast

    Mutation

    Saccharomyces cerevisiae recode 1

    Promoter

    pGAL1

    Available Since

    April 29, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. pGBW-m4046589

    Plasmid

    #145450

    Purpose

    Yeast Expression plasmid for SARS-CoV-2 ORF6 protein

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 ORF6 protein (ORF6 Severe acute respiratory syndrome coronavirus 2, Budding Yeast)
    

    Tags

    Cleavable thrombin;6xHIS

    Expression

    Yeast

    Mutation

    Saccharomyces cerevisiae recode 1

    Promoter

    pGAL1

    Available Since

    April 29, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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16. pDN-G1GZmbh

    Plasmid

    #44516

    Depositor

    Gabor Balazsi

    Article

    Nevozhay et al Proc Natl Acad Sci U S A. 2009 Mar 31;10

    Inserts

    PGal1-D12 promoter (GAL1 Synthetic, Budding Yeast)
    yEGFP::ZeoR
    

    Use

    Synthetic Biology; Expression regulator/reporter

    Expression

    Yeast

    Mutation

    Two tandem tet operators (2XtetO2) downstream of …

    Promoter

    PGal1-D12 promoter

    Available Since

    April 23, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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17. pI4-MTU-DO-HIS

    Plasmid

    #160026

    Purpose

    Integrative vector for K. marxianus, targeting integration site I4 described in (Rajkumar et al.,2019). Histidine selection.

    Depositor

    John Morrissey

    Article

    Rajkumar et al Microb Cell Fact. 2020 Nov 11;19(1):207.

    Type

    Empty backbone

    Use

    Kluyveromyces marxianus

    Expression

    Yeast

    Promoter

    n/a

    Available Since

    March 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pI2-MTU-DO-HPH

    Plasmid

    #160019

    Purpose

    Integrative vector for K. marxianus, targeting integration site I2 described in (Rajkumar et al.,2019). Hygromycin selection.

    Depositor

    John Morrissey

    Article

    Rajkumar et al Microb Cell Fact. 2020 Nov 11;19(1):207.

    Type

    Empty backbone

    Use

    Kluyveromyces marxianus

    Expression

    Yeast

    Promoter

    n/a

    Available Since

    Nov. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pI2-MTU-DO-LEU

    Plasmid

    #160017

    Purpose

    Integrative vector for K. marxianus, targeting integration site I2 described in (Rajkumar et al.,2019). Leucine selection.

    Depositor

    John Morrissey

    Article

    Rajkumar et al Microb Cell Fact. 2020 Nov 11;19(1):207.

    Type

    Empty backbone

    Use

    Kluyveromyces marxianus

    Expression

    Yeast

    Promoter

    n/a

    Available Since

    Nov. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. YIplac211-SNX4-iGFPx3

    Plasmid

    #105269

    Purpose

    Plasmid for pop-in/pop-out replacement of Snx4 with Snx4-iGFPx3.

    Depositor

    Benjamin Glick

    Article

    Day et al Dev Cell. 2018 Jan 8;44(1):56-72.e4. doi

    Insert

    Snx4 portion (SNX4 Budding Yeast)
    

    Tags

    iGFPx3

    Expression

    Yeast

    Available Since

    Jan. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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