Addgene

Showing: 3241 - 3260 of 7892 results

Results per page:

Sort results by:

pGPDluxAB(Ura)

Plasmid

#1119

Depositor

Susan Lindquist

Article

Singer et al Mol Cell 1998 Apr;1(5):639-48.

Insert

bacterial luciferase

Use

Tags

Expression

Yeast

Mutation

n/a

Promoter

Available since

May 24, 2006

Availability

Academic Institutions and Nonprofits only
GTT-r

Plasmid

#81111

Purpose

Gene Tagging vector TRP/RFP - Plasmid for monomeric Cherry (mCherry) labeling of endogenous proteins with a TRP selection marker, originating from pSIVt (ID:81092)

Depositor

Serge Pelet

Article

Wosika et al Mol Genet Genomics. 2016 Dec;291(6):2231

Insert

mCherry

Use

Cre/Lox

Tags

mCherry

Expression

Yeast

Mutation

Promoter

Available since

Nov. 16, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
LD221

Plasmid

#69189

Purpose

Integration of 2GFP tag at Atg21 C terminus, use auxotrophic marker URA3(Candida albicans)

Depositor

Zhiping Xie

Article

Li et al Autophagy. 2015;11(6):954-60. doi: 10.10

Insert

Atg21-2EGFP

Use

Tags

2xEGFP

Expression

Yeast

Mutation

Promoter

Available since

Oct. 28, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
413-Met25 Kar2 TorsinADE GFP

Plasmid

#32120

Depositor

Susan Lindquist

Article

Valastyan et al PLoS One. 2011;6(7):e22744. Epub 2011 Ju

Insert

TorsinA (TOR1A Human)

Use

Tags

GFP and Kar2

Expression

Yeast

Mutation

deletion of E at 302 or 303

Promoter

Available since

Sept. 30, 2011

Availability

Academic Institutions and Nonprofits only
pNB655

Plasmid

#60142

Purpose

Firefly luciferase (Promega) yeast codon-optimized yellow fluorescent protein (yEVenus) fusion driven by MET17 promoter.

Depositor

Nicolas Buchler

Article

Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

Insert

FLuc-yEVenus

Use

Tags

Expression

Yeast

Mutation

A4V

Promoter

MET17pr

Available since

Feb. 7, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pRM102

Plasmid

#44525

Depositor

Michael Grunstein

Article

Mann et al EMBO J. 1992 Sep;11(9):3297-306.

Insert

P(GAL10)-HHT2 P(GAL1)-HHF2

Use

Tags

Expression

Yeast

Mutation

Promoter

GAL 10

Available since

May 22, 2013

Availability

Academic Institutions and Nonprofits only
MCA2-eGFP

Plasmid

#117098

Purpose

protein expression in yeast

Depositor

Geoffrey Chang

Insert

MCA2-eGFP

Use

Tags

His

Expression

Yeast

Mutation

Promoter

AOX

Available since

Nov. 6, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
MCA1-eGFP

Plasmid

#117097

Purpose

protein expression in yeast

Depositor

Geoffrey Chang

Insert

MCA1-eGFP

Use

Tags

His

Expression

Yeast

Mutation

Promoter

AOX

Available since

Nov. 6, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pCfB2400

Plasmid

#67552

Purpose

EasyClone system-based yeast integrative vector carrying loxP-flanked dsdA marker, integration into S. cerevisiae XII-1 chromosomal location, USER site for cloning, amp resistance

Depositor

Irina Borodina

Article

Stovicek et al J Ind Microbiol Biotechnol. 2015 Sep 16.

Type

Empty backbone

Use

Cre/Lox; Integrative

Tags

Expression

Yeast

Mutation

Promoter

Available since

March 10, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pYEXNHG3

Plasmid

#49025

Purpose

FX cloning Saccharomyces cerevisiae expression vector with GAL1 promoter and N-terminal 10x His tag, GFP and 3C protease cleavage site

Depositor

Eric Geertsma, Raimund Dutzler

Article

Geertsma et al Biochemistry. 2011 Apr 19;50(15):3272-8.

Type

Empty backbone

Use

Tags

3C protease site (PreScission site), GFP, and dec…

Expression

Yeast

Mutation

Promoter

GAL1

Available since

Jan. 15, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pLEP1-H-Hsp82

Plasmid

#1277

Depositor

Susan Lindquist

Article

Chang et al J Biol Chem 1994 Oct 7;269(40):24983-8.

Insert

his-Hsp82 (HSP82 Budding Yeast)

Use

Tags

Histidinex6

Expression

Yeast

Mutation

Promoter

Available since

July 18, 2005

Availability

Academic Institutions and Nonprofits only
pCfB8382

Plasmid

#126916

Purpose

Plasmid containing integration cassette at XI-3 integration site in Saccharomyces cerevisiae for overexpression of XdCrtI

Depositor

Irina Borodina

Article

Milne et al FEMS Yeast Res. 2019 Sep 26. pii: foz062

Insert

XdCrtI

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Dec. 17, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pDS255

Plasmid

#34970

Depositor

Michael Glotzer

Article

Strickland et al Nat Methods. 2012 Mar 4. doi: 10.1038/nm

Insert

Mid2(SS/TM)-GFP-LOVpep

Use

Tags

Expression

Yeast

Mutation

T406A, T407A

Promoter

Available since

March 23, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
pXRkMX

Plasmid

#63147

Purpose

Kan marker pXR vector uses for both plasmid assembly in vivo, as well as targeted genomic integration.

Depositor

Marc Gartenberg

Article

Chou et al FEMS Yeast Res. 2015 May;15(3):fov010. d

Type

Empty backbone

Use

Cre/Lox

Tags

Expression

Bacterial and Yeast

Mutation

Promoter

Available since

April 3, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
POM43-mCitrine

Plasmid

#69855

Purpose

N-terminal tagging with mCitrine using LEU2 as a selection marker in S. cerevisiae.

Depositor

Liza Pon

Article

Higuchi-Sanabria et al PLoS One. 2016 Jan 4;11(1):e0146120. doi

Insert

mCitrine minus start and stop codon

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

April 16, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pRS408

Plasmid

#11255

Depositor

Fred Cross

Type

Empty backbone

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Feb. 15, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
pRS316Nab3

Plasmid

#110533

Purpose

Expresses yeast NAB3 using the endogenous promoter

Depositor

Daniel Reines

Article

Loya et al Nucleic Acids Res. 2012 Aug;40(15):7476-

Insert

NAB3 (NAB3 Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

June 19, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pCfB3043(gRNA XI-1)

Plasmid

#73285

Purpose

EasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at site XI-1

Depositor

Irina Borodina

Article

Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

Insert

guiding RNA

Use

CRISPR; Grna

Tags

Expression

Yeast

Mutation

Promoter

Available since

Nov. 23, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pR5-30

Plasmid

#22290

Depositor

Louise Prakash

Article

Pages et al Genetics. 2008 Sep . 180(1):73-82.

Insert

RAD5 (RAD5 Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

D681, E682->AA

Promoter

Available since

Nov. 10, 2009

Availability

Academic Institutions and Nonprofits only
pR5-19

Plasmid

#22288

Depositor

Louise Prakash

Article

Pages et al Genetics. 2008 Sep . 180(1):73-82.

Insert

RAD5 (RAD5 Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

C914, C917->AA in the C3HC4 ring-finger motif

Promoter

Available since

Nov. 2, 2009

Availability

Academic Institutions and Nonprofits only