8,086 results

1. pRS408

   Plasmid

   #11255

   Depositor

   Fred Cross

   Type

   Empty backbone

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   Feb. 15, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLEP1-H-Hsp82

   Plasmid

   #1277

   Depositor

   Susan Lindquist

   Article

   Chang et al J Biol Chem 1994 Oct 7;269(40):24983-8.

   Insert

   his-Hsp82 (HSP82 Budding Yeast)
   

   Use

   Tags

   Histidinex6

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   July 18, 2005

   Availability

   Academic Institutions and Nonprofits only

3. pBCSK_HIS3_II

   Plasmid

   #109059

   Purpose

   yeast shuttle vector with deletable CEN6-ARSH4 cassette. For teaching purposes.

   Depositor

   Colin Logie

   Insert

   HIS3 (HIS3 Zygosaccharomyces rouxii, Budding Yeast)
   

   Use

   Cre/Lox

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   HIS3

   Available Since

   Aug. 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pFA1

   Plasmid

   #46789

   Purpose

   To express Fun30 in trans in budding yeast

   Depositor

   Patrick Varga-Weisz

   Article

   Neves-Costa et al PLoS One. 2009 Dec 1;4(12):e8111. doi: 1

   Insert

   FUN30 (FUN30 Budding Yeast)
   

   Use

   Tags

   V5

   Expression

   Yeast

   Mutation

   Promoter

   PGAL1

   Available Since

   Aug. 6, 2013

   Availability

   Academic Institutions and Nonprofits only

5. pDS255

   Plasmid

   #34970

   Depositor

   Michael Glotzer

   Article

   Strickland et al Nat Methods. 2012 Mar 4. doi: 10.1038/nm

   Insert

   Mid2(SS/TM)-GFP-LOVpep
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   T406A, T407A

   Promoter

   Available Since

   March 23, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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6. GTT-r

   Plasmid

   #81111

   Purpose

   Gene Tagging vector TRP/RFP - Plasmid for monomeric Cherry (mCherry) labeling of endogenous proteins with a TRP selection marker, originating from pSIVt (ID:81092)

   Depositor

   Serge Pelet

   Article

   Wosika et al Mol Genet Genomics. 2016 Dec;291(6):2231

   Insert

   mCherry
   

   Use

   Cre/Lox

   Tags

   mCherry

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   Nov. 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pYEXNHG3

   Plasmid

   #49025

   Purpose

   FX cloning Saccharomyces cerevisiae expression vector with GAL1 promoter and N-terminal 10x His tag, GFP and 3C protease cleavage site

   Depositor

   Raimund Dutzler, Eric Geertsma

   Article

   Geertsma et al Biochemistry. 2011 Apr 19;50(15):3272-8.

   Type

   Empty backbone

   Use

   Tags

   3C protease site (PreScission site), GFP, and dec…

   Expression

   Yeast

   Mutation

   Promoter

   GAL1

   Available Since

   Jan. 15, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pFA6a-kanMX6-P41nmt1-mYFP

   Plasmid

   #105141

   Purpose

   Yeast gene targeting

   Depositor

   Jian-Qiu Wu, Thomas Pollard

   Article

   Wu et al Dev Cell. 2003 Nov;5(5):723-34.

   Insert

   kanMX6-P41nmt1-mYFP
   

   Use

   Tags

   41nmt1-mYFP

   Expression

   Bacterial and Yeast

   Mutation

   A206K

   Promoter

   41nmt1

   Available Since

   March 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pVEG17*

   Plasmid

   #111856

   Purpose

   Xylose-responsive Gal3p-Syn4.1 under control of native GAL1p (promoter)

   Depositor

   Nikhil Nair

   Article

   Endalur Gopinarayanan et al Nat Commun. 2018 Mar 26;9(1):1233. doi:

   Insert

   GAL1p-GAL3-Syn4.1-TEFt
   

   Use

   Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   D68N, V69M, A109V, I271L

   Promoter

   GAL1p

   Available Since

   June 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pRG711_lexO-AscI_LexA-TF_HIS3MX

    Plasmid

    #154820

    Purpose

    Inducible expression of AscI endonuclease in S. cerevisiae

    Depositor

    Lorraine Symington

    Article

    Gnugge et al Nucleic Acids Res. 2020 Oct 14. pii: 592

    Inserts

    AscI endonuclease
    LexA-ER-B112
    

    Use

    Tags

    FLAG and NLS

    Expression

    Yeast

    Mutation

    Promoter

    ACT1 and lexO_4-CYC1_core

    Available Since

    Oct. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pXP316

    Plasmid

    #26836

    Depositor

    Nancy DaSilva, Suzanne Sandmeyer

    Article

    Fang et al Yeast. 2011 Feb;28(2):123-36. doi: 10.10

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Dec. 8, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCTCON2-BXG-2TAG

    Plasmid

    #158138

    Purpose

    Dual fluorescent protein reporter (BFP-GFP) for ncAA incorporation (2 TAG construct)

    Depositor

    James Van Deventer

    Article

    Potts et al Mol Syst Des Eng. 2020 Feb 1;5(2):573-58

    Insert

    BFP-GFP fragment
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Cloned in a TAG codon at the first serine residue…

    Promoter

    GAL1-10

    Available Since

    Jan. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. YIplac211-KEX2-iGFPx6

    Plasmid

    #105264

    Purpose

    Plasmid for pop-in/pop-out replacement of Kex2 with Kex2-iGFP-x6.

    Depositor

    Benjamin Glick

    Article

    Day et al Dev Cell. 2018 Jan 8;44(1):56-72.e4. doi

    Insert

    Kex2 portion (KEX2 )
    

    Use

    Tags

    iGFPx6

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Jan. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDDGFP-Leu2d_GgPCFT

    Plasmid

    #165414

    Purpose

    expression GgPCFT protein with C terminally tagged GFP-His tag cleavable with TEV protease.

    Depositor

    Simon Newstead

    Article

    Parker et al Nature. 2021 May 26. pii: 10.1038/s41586

    Insert

    GgPCFT (SLC46A1 Chicken, Synthetic)
    

    Use

    Tags

    GFP-His8

    Expression

    Yeast

    Mutation

    Promoter

    GAL1

    Available Since

    July 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCSN069

    Plasmid

    #166728

    Purpose

    Single copy yeast plasmid expressing DNase-dead dLbCas12a E925A fused to Mxi1, codon optimized for Saccharomyces cerevisiae

    Depositor

    Rene Verwaal

    Article

    Ciurkot et al Nucleic Acids Res. 2021 Jul 1. pii: 6312

    Insert

    dCas12a-Mxi1
    

    Use

    CRISPR

    Tags

    Mxi1

    Expression

    Yeast

    Mutation

    E925A

    Promoter

    S. cerevisiae TEF1

    Available Since

    June 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pPS892

    Plasmid

    #8908

    Depositor

    Pamela Silver

    Article

    Koepp et al J Cell Biol. 1996 Jun . 133(6):1163-76.

    Type

    Empty backbone

    Use

    Tags

    GST

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Aug. 19, 2005

    Availability

    Academic Institutions and Nonprofits only

17. pR5-30

    Plasmid

    #22290

    Depositor

    Louise Prakash

    Article

    Pages et al Genetics. 2008 Sep . 180(1):73-82.

    Insert

    RAD5 (RAD5 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    D681, E682->AA

    Promoter

    Available Since

    Nov. 10, 2009

    Availability

    Academic Institutions and Nonprofits only

18. pR5-19

    Plasmid

    #22288

    Depositor

    Louise Prakash

    Article

    Pages et al Genetics. 2008 Sep . 180(1):73-82.

    Insert

    RAD5 (RAD5 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    C914, C917->AA in the C3HC4 ring-finger motif

    Promoter

    Available Since

    Nov. 2, 2009

    Availability

    Academic Institutions and Nonprofits only

19. pXRkMX

    Plasmid

    #63147

    Purpose

    Kan marker pXR vector uses for both plasmid assembly in vivo, as well as targeted genomic integration.

    Depositor

    Marc Gartenberg

    Article

    Chou et al FEMS Yeast Res. 2015 May;15(3):fov010. d

    Type

    Empty backbone

    Use

    Cre/Lox

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available Since

    April 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pNC891

    Plasmid

    #20397

    Depositor

    Beverly Errede

    Article

    Hackett et al Yeast. 2006 Apr 15. 23(5):333-49.

    Insert

    YFP-reporter
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    X=M (see schematic)

    Promoter

    Available Since

    March 6, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge