8,175 results
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Plasmid#106157PurposeEasyCloneYALI system-based yeast integrative vector for markerfree integration via CRISPR/Cas9 to be used in combination with pCfB6637, integration into Yarrowia lipolytica chromosomal location IntE_3, USER site for cloning, amp resistanceDepositorTypeEmpty backboneExpressionYeastAvailable SinceMay 11, 2018AvailabilityAcademic Institutions and Nonprofits only
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pCfB3053(gRNA X-2, XI-5, XII-4)
Plasmid#73295PurposeEasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at sites X-2, XI-5, and XII-4DepositorInsertguiding RNA
UseCRISPR; GrnaExpressionYeastAvailable SinceNov. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pXRH3
Plasmid#63145PurposeHis3 marker pXR vector uses for both plasmid assembly in vivo, as well as targeted genomic integration.DepositorTypeEmpty backboneUseCre/LoxExpressionBacterial and YeastAvailable SinceApril 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
pJK371
Plasmid#73596PurposeEncodes Crepis alpina delta-12 fatty acid acetylenase (vFAD2) with C-terminal FLAG epitope and delta-12 fatty acid desaturase (FAD2-3) with C-terminal c-myc epitopeDepositorInsertsdelta-12 fatty acid acetylenase
delta-12 fatty acid desaturase (form 3)
TagsFLAG tag and c-myc tagExpressionYeastMutationPoint mutants in FAD2-3 ORF relative to deposited…PromoterGAL1 and GAL10Available SinceMarch 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
p413TEF-HST2
Plasmid#51745Purposeoverexpression of HST2 in S. cerevisiae, HIS3 markerDepositorAvailable SinceMarch 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
pYEXC3H
Plasmid#49026PurposeFX cloning Saccharomyces cerevisiae expression vector with GAL1 promoter and C-terminal 3C protease cleavage site and 10x His tagDepositorTypeEmpty backboneTags3C protease site (PreScission site) and decaHis (…ExpressionYeastPromoterGAL1Available SinceJan. 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
pXP114
Plasmid#26812DepositorTypeEmpty backboneUseCre/LoxExpressionYeastAvailable SinceDec. 8, 2010AvailabilityAcademic Institutions and Nonprofits only -
ymScarletI-ymNeongreen pDRF1-GW
Plasmid#168067PurposeFRET fusion between ymScarletI and ymNeongreen for use in yeastDepositorInsertymScarletI-ymNeongreen
ExpressionYeastAvailable SinceJune 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pXP316
Plasmid#26836DepositorTypeEmpty backboneExpressionYeastAvailable SinceDec. 8, 2010AvailabilityAcademic Institutions and Nonprofits only -
pGY466
Plasmid#166661PurposeFor light-inducible Cre recombinase (LiCre) expression under the Met17 promoter in yeastDepositorInsertLiCre
UseCre/LoxExpressionYeastMutationCre: E340A, D341APromoterMET17Available SinceApril 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pYEXNHG3
Plasmid#49025PurposeFX cloning Saccharomyces cerevisiae expression vector with GAL1 promoter and N-terminal 10x His tag, GFP and 3C protease cleavage siteDepositorTypeEmpty backboneTags3C protease site (PreScission site), GFP, and dec…ExpressionYeastPromoterGAL1Available SinceJan. 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
pNX3a-PK3
Plasmid#78346Purposefission yeast epitope taggingDepositorInserts3xPK
kanMX6
ExpressionYeastAvailable SinceNov. 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
pICE MET17
Plasmid#122880PurposeMET17 ICE plasmid used to facilitate yeast recombination cloning and stable genomic Integration after CEN Excision.DepositorTypeEmpty backboneUseYeast recombination cloningExpressionYeastAvailable SinceJan. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
CUP Puf2 YFP p316
Plasmid#15595DepositorAvailable SinceAug. 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
GTT-r
Plasmid#81111PurposeGene Tagging vector TRP/RFP - Plasmid for monomeric Cherry (mCherry) labeling of endogenous proteins with a TRP selection marker, originating from pSIVt (ID:81092)DepositorInsertmCherry
UseCre/LoxTagsmCherryExpressionYeastAvailable SinceNov. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pILGFP86
Plasmid#83543PurposeUsed to evaluate the expression output of RPL4A promoter with yEGFP used as the reporter gene.DepositorInsertRPL4A promoter-yEGFP
ExpressionYeastPromoterRPL4AAvailable SinceDec. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMM0647
Plasmid#129008PurposeIntegration vector for optogenetic machinery (pMEDIUM-VP16-CIB1/pMEDIUM-ZCRY2PHR) and pZF-mRUBY2 with loxable KIURA3 markerDepositorInsertKanR-ColE1 URA 5'-pRPL18B-SV40NLS-VP16-CIB1-tENO1-pRPL18B-SV40NLS-ZCRY2PHR-tSSA1--pZF(BS)-mRUBY2-tADH1-loxP-KIURA3-loxP-URA3 3'
ExpressionYeastAvailable SinceOct. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMM0623
Plasmid#128998PurposepRPL18B-Zif268DBD-CRY2PHR cassette plasmid flanked by ConL1 and ConR2DepositorInsertConL1-pRPL18B-SV40NLS-ZiF268DBD-CRY2PHR-tSSA1-ConR2 AmpR-ColE1
ExpressionYeastAvailable SinceOct. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMM0624
Plasmid#128999PurposepZF(3BS)-mRUBY2 cassette plasmid flanked by ConL2 and ConREDepositorInsertConL2-pZF(BS)-mRUBY2-tADH1-ConRE AmpR-ColE1
ExpressionYeastAvailable SinceOct. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMM0621
Plasmid#128996PurposepRPL18B-VP16-CIB1 cassette plasmid flanked by ConLS and ConR1DepositorInsertConLS_pRPL18B-SV40NLS-VP16-CIB1-tENO1-ConR1 AmpR Col-E1
ExpressionYeastAvailable SinceOct. 7, 2019AvailabilityAcademic Institutions and Nonprofits only