8,086 results

1. pPS2045

   Plasmid

   #1376

   Depositor

   Pamela Silver

   Article

   Brodsky et al RNA 2000 Dec;6(12):1737-49.

   Insert

   U1A (1-94) (RNU1-4 Human)
   

   Use

   Tags

   GFP

   Expression

   Yeast

   Mutation

   U1A (1-94)

   Promoter

   Available Since

   Aug. 3, 2005

   Availability

   Academic Institutions and Nonprofits only

2. pRS316Nab3

   Plasmid

   #110533

   Purpose

   Expresses yeast NAB3 using the endogenous promoter

   Depositor

   Daniel Reines

   Article

   Loya et al Nucleic Acids Res. 2012 Aug;40(15):7476-

   Insert

   NAB3 (NAB3 Budding Yeast)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   June 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. YIp ADE2-MET17

   Plasmid

   #122906

   Purpose

   ADE2-MET17 integrating (YIp) vector for stable genomic integration in the BY4741 background.

   Depositor

   Randy Hampton

   Article

   Flagg et al Yeast. 2019 May 10. doi: 10.1002/yea.340

   Type

   Empty backbone

   Use

   Yeast recombination cloning

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   Aug. 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAG425GPD-ccdB-ECFP

   Plasmid

   #14178

   Depositor

   Susan Lindquist

   Article

   Alberti et al Yeast. 2007 Oct . 24(10):913-9.

   Type

   Empty backbone

   Use

   Gateway destination

   Tags

   ECFP

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   March 16, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEM01

   Plasmid

   #135414

   Purpose

   Expression of Hygromycin resistance from CMV promoter

   Depositor

   Nicolas Buchler

   Article

   Medina et al Elife. 2020 May 11;9. pii: 52741. doi: 1

   Insert

   CMVpr hph ADH1t
   

   Use

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   CMVpr

   Available Since

   May 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMZ381

   Plasmid

   #59899

   Purpose

   Combination adh1:cas9/rrk1:sgRNA for CRISPR genome editing in fission yeast: sgRNA targeted against ade6-L469

   Depositor

   Mikel Zaratiegui

   Article

   Jacobs et al Nat Commun. 2014 Oct 29;5:5344. doi: 10.

   Inserts

   Cas9
   rrk1:sgRNA
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Silent muation of the CspCI site

   Promoter

   Available Since

   Oct. 29, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pREP4xN_ccdB

   Plasmid

   #79022

   Purpose

   Gateway destination vector for S. pombe. Allow N-terminal tag(s) to be introduced using MCS. attR1-CmR-ccdB-attR2 cassette, nmt1 (ATATATAAA) promoter, ura4+ selection

   Depositor

   Pernilla Bjerling, Göran Akusjärvi

   Type

   Empty backbone

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   July 7, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pRS406-PHO5-GFP-Scs2

   Plasmid

   #58834

   Purpose

   Expresses GFP-Tagged Scs2 in yeast

   Depositor

   Tim Levine

   Article

   Loewen et al EMBO J. 2003 May 1;22(9):2025-35.

   Insert

   Scs2 (SCS2 Budding Yeast)
   

   Use

   Tags

   GFP and Myc

   Expression

   Yeast

   Mutation

   Promoter

   PHO5

   Available Since

   Aug. 27, 2014

   Availability

   Academic Institutions and Nonprofits only

9. pAG413GAL-Cerulean-ccdB

   Plasmid

   #14405

   Depositor

   Susan Lindquist

   Article

   Alberti et al Yeast. 2007 Oct . 24(10):913-9.

   Type

   Empty backbone

   Use

   Gateway destination

   Tags

   Cerulean

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   June 18, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pJT45_GalL_nCDA1-BE3

    Plasmid

    #145038

    Purpose

    Expresses nCDA1-BE3 in yeast cells

    Depositor

    Ralph Bock

    Article

    Tan et al Nat Commun. 2019 Jan 25;10(1):439. doi:

    Insert

    nCDA1-BE3
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    spCas9(D10A)

    Promoter

    GalL

    Available Since

    Sept. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pJAID5VY

    Plasmid

    #165048

    Purpose

    Integrative expression of CUP1-AID* (mini auxin inducible degron)-yEGFP and rice auxin receptor gene OsTIR1.

    Depositor

    Claudia Vickers

    Article

    Lu et al Nat Commun. 2021 Feb 16;12(1):1051. doi:

    Insert

    P-URA3>KlURA3>T-AgTEF1-P-TEF1>CUP1-AID*>yEGFP>T-PGK1-P-ACS2>OsTIR1opt>T-URA3
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    OsTIR1 is codon-optimised for expression in S. ce…

    Promoter

    Available Since

    March 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pPIC-mouseKir3.2-mC-S

    Plasmid

    #140745

    Purpose

    Expression of mouse Kir3.2 fused to mCherry and Streptag II

    Depositor

    Arthur Laganowsky

    Article

    Qiao et al Biochemistry. 2020 May 6. doi: 10.1021/a

    Insert

    Kir3.2 (Kcnj6 Mouse)
    

    Use

    Tags

    mCherry with Streptag II

    Expression

    Yeast

    Mutation

    Promoter

    AOX1

    Available Since

    May 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pPTK015-3-yEGFP_Intra

    Plasmid

    #84974

    Purpose

    Type 3, Green fluorescent protein

    Depositor

    Volker Sieber

    Article

    Obst et al ACS Synth Biol. 2017 Mar 15. doi: 10.102

    Insert

    Green fluorescent protein
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Non

    Available Since

    April 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGBW-m4046380

    Plasmid

    #148977

    Purpose

    Yeast Expression plasmid for SARS-CoV-2 E (envelope)

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 E (envelope) (E Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Cleavable thrombin;MBP

    Expression

    Yeast

    Mutation

    Saccharomyces cerevisiae recode 1

    Promoter

    pGAL1

    Available Since

    May 14, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. pGBW-m4046710

    Plasmid

    #149178

    Purpose

    Yeast Expression plasmid for SARS-CoV-2 E (envelope)

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 E (envelope) (E Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    wt

    Promoter

    pTEF1

    Available Since

    May 5, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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16. pDRF’- AmTryoshka1;3-GS

    Plasmid

    #100967

    Purpose

    Yeast expression of control vector for fluorescent sensors reporting the activity of ammonium transceptors.

    Depositor

    Wolf Frommer

    Article

    Ast et al Nat Commun. 2017 Sep 5;8(1):431. doi: 10

    Insert

    AmTryoshka1;3-GS (AMT1;3 Mustard Weed)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    PMA

    Available Since

    Oct. 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pWS033

    Plasmid

    #123056

    Purpose

    Green fluorescent protein

    Depositor

    Tom Ellis

    Article

    Shaw et al Cell. 2019 Apr 18;177(3):782-796.e27. do

    Insert

    sfGFP [3]
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    April 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pAG415GPD-Cerulean-ccdB

    Plasmid

    #14410

    Depositor

    Susan Lindquist

    Article

    Alberti et al Yeast. 2007 Oct . 24(10):913-9.

    Type

    Empty backbone

    Use

    Gateway destination

    Tags

    Cerulean

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    June 18, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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19. pFA6a-kanMX6-P41nmt1-mEGFP

    Plasmid

    #105140

    Purpose

    Yeast gene targeting

    Depositor

    Jian-Qiu Wu

    Article

    Laporte et al Mol Biol Cell. 2012 Aug;23(16):3094-110.

    Insert

    kanMX6-P41nmt1-mEGFP
    

    Use

    Tags

    41nmt1-mEGFP

    Expression

    Bacterial and Yeast

    Mutation

    A206K

    Promoter

    41nmt1

    Available Since

    Feb. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pNIA-CEN-Venus-FLAG-LINXa4-Bre1

    Plasmid

    #73618

    Purpose

    Visualizing the nuclear translocation of LINXa4-Bre1 in yeast.

    Depositor

    Brian Kuhlman

    Article

    Yumerefendi et al Nat Chem Biol. 2016 Jun;12(6):399-401. d

    Insert

    Venus
    

    Use

    Synthetic Biology

    Tags

    FLAG

    Expression

    Yeast

    Mutation

    Bre1 point mutations K8A, K9A and K11A

    Promoter

    ADH1

    Available Since

    April 27, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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