8,060 results

1. pFA3

   Plasmid

   #46790

   Purpose

   To express ATPase inactive Fun30 in budding yeast

   Depositor

   Patrick Varga-Weisz

   Article

   Neves-Costa et al PLoS One. 2009 Dec 1;4(12):e8111. doi: 1

   Insert

   FUN30 (FUN30 Budding Yeast)
   

   Use

   Tags

   V5

   Expression

   Yeast

   Mutation

   ATP-binding site mutated replaced lysin 603 by ar…

   Promoter

   PGAL1

   Available Since

   Aug. 7, 2013

   Availability

   Academic Institutions and Nonprofits only

2. pAG305GAL-ccdB-EYFP

   Plasmid

   #14209

   Depositor

   Susan Lindquist

   Article

   Alberti et al Yeast. 2007 Oct . 24(10):913-9.

   Type

   Empty backbone

   Use

   Gateway destination

   Tags

   EYFP

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   March 16, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. YIp TRP1-URA3

   Plasmid

   #122915

   Purpose

   TRP1-URA3 integrating (YIp) vector for stable genomic integration in the BY4741 background.

   Depositor

   Randy Hampton

   Article

   Flagg et al Yeast. 2019 May 10. doi: 10.1002/yea.340

   Type

   Empty backbone

   Use

   Yeast recombination cloning

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   July 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pPTK008-3a-αMFΔ no Kex

   Plasmid

   #84967

   Purpose

   Type 3a, α-mating factor no Kex recognition site - secretion signal

   Depositor

   Volker Sieber

   Article

   Obst et al ACS Synth Biol. 2017 Mar 15. doi: 10.102

   Insert

   α-mating factor no Kex recognition site secretion tag
   

   Use

   Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Non

   Available Since

   April 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pILGFPQ3

   Plasmid

   #83558

   Purpose

   Used to evaluate the expression output of GAL2 promoter with yEGFP used as the reporter gene.

   Depositor

   Claudia Vickers

   Article

   Peng et al Biotechnol Biofuels. 2017 Feb 21;10:43.

   Insert

   GAL2 promoter-yEGFP
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   GAL2

   Available Since

   Dec. 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAG416GPD-Cerulean-CHC1

   Plasmid

   #18850

   Depositor

   Susan Lindquist

   Article

   Gitler et al Proc Natl Acad Sci U S A. 2008 Jan 8. 10

   Insert

   CHC1 (CHC1 Budding Yeast)
   

   Use

   Tags

   Cerulean

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   Nov. 21, 2008

   Availability

   Academic Institutions and Nonprofits only

7. pFA6a-PAmKate-KanMX6

   Plasmid

   #87028

   Purpose

   Tag S. pombe genes in C terminal with PAmKate

   Depositor

   Julien Berro

   Insert

   PAmKate
   

   Use

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   Available Since

   Feb. 22, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pFA6a-his3-P3nmt1-3FLAG

   Plasmid

   #19339

   Depositor

   Eishi Noguchi

   Article

   Noguchi et al Biotechnol J. 2008 Oct;3(9-10):1280-5. d

   Insert

   his3 (his3 Fission Yeast)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   Sept. 19, 2008

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pTSK241

   Plasmid

   #34550

   Depositor

   James McNally

   Article

   Karpova et al Science. 2008 Jan 25;319(5862):466-9.

   Insert

   Ace1 (CUP2 Budding Yeast)
   

   Use

   Integrative plasmid

   Tags

   GFP

   Expression

   Yeast

   Mutation

   Promoter

   CAP2

   Available Since

   Jan. 24, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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10. pPS832

    Plasmid

    #1357

    Depositor

    Pamela Silver

    Article

    Schlenstedt et al Proc Natl Acad Sci U S A 1995 Jan 3;92(1

    Insert

    GSP1 (GSP1 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Aug. 3, 2005

    Availability

    Academic Institutions and Nonprofits only

11. pPS833

    Plasmid

    #1358

    Depositor

    Pamela Silver

    Article

    Schlenstedt et al Proc Natl Acad Sci U S A 1995 Jan 3;92(1

    Insert

    GSP1 (GSP1 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    G21V, stabilizes the GTP-bound form

    Promoter

    Available Since

    Aug. 3, 2005

    Availability

    Academic Institutions and Nonprofits only

12. pGBW-m4046534

    Plasmid

    #145571

    Purpose

    Yeast Expression plasmid for SARS-CoV-2 ORF10 protein

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 ORF10 protein (ORF10 Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Cleavable thrombin;MBP

    Expression

    Yeast

    Mutation

    wt

    Promoter

    pGAL1

    Available Since

    April 29, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

13. YIp204-P30-His-POL30

    Plasmid

    #99550

    Purpose

    Yeast integrative vector for expression of His-tagged POL30 (PCNA); TRP1 marker

    Depositor

    Helle Ulrich

    Article

    Davies et al Methods Mol Biol. 2012;920:543-67. doi:

    Insert

    POL30 (POL30 Budding Yeast)
    

    Use

    Tags

    6xHis

    Expression

    Yeast

    Mutation

    Promoter

    POL30

    Available Since

    Sept. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGBW-m4046495

    Plasmid

    #148983

    Purpose

    Yeast Expression plasmid for SARS-CoV-2 nsp8

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 nsp8 (ORF1ab Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Cleavable thrombin;MBP

    Expression

    Yeast

    Mutation

    wt

    Promoter

    pTEF1

    Available Since

    May 14, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. pPS1891

    Plasmid

    #1363

    Depositor

    Pamela Silver

    Article

    Damelin et al Mol Cell 2000 Jan;5(1):133-40.

    Insert

    NLS-EYFP
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    May 24, 2006

    Availability

    Academic Institutions and Nonprofits only

16. PSUC2-GFPantisense-CYC1t-pRS413

    Plasmid

    #64404

    Purpose

    SUC2 regulated expression of an antisense RNAi construct for GFP

    Depositor

    Claudia Vickers

    Article

    Williams et al Microb Cell Fact. 2015 Apr 1;14:43. doi:

    Insert

    GFPantisense
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    SUC2

    Available Since

    June 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCP5b

    Plasmid

    #37185

    Depositor

    Adam Bogdanove

    Article

    Cermak et al Nucleic Acids Res. 2011 Jul;39(12):e82.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Dec. 6, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pRS415-BUD32

    Plasmid

    #10991

    Depositor

    Francisco Malagon

    Insert

    BUD32 (BUD32 Budding Yeast)
    

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Wild type

    Promoter

    Available Since

    Dec. 29, 2005

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. p426 1-F GW

    Plasmid

    #58548

    Purpose

    pRS426 carrying Gateway cassette for a single insert

    Depositor

    Leonid Kruglyak

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Sept. 26, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pRS303-pGAL-SPPHO5-G-SrtAstrep-HA-HDEL (pKS82)

    Plasmid

    #50032

    Purpose

    Galactose-inducible expression of ER-luminal S. pyogenes SrtA in yeast

    Depositor

    Hidde Ploegh

    Article

    Strijbis et al Traffic. 2012 Jun;13(6):780-9. doi: 10.1

    Insert

    HA-SrtA(strep)
    

    Use

    Tags

    HA-HDEL

    Expression

    Yeast

    Mutation

    Promoter

    pGAL

    Available Since

    Jan. 26, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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