8,430 results
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Plasmid#184760PurposeCDS1 overexpression. Diverts glycerolipid synthesis pathway towards phospholipids. Uses antibiotic resistance marker hphMX6.DepositorAvailable sinceFeb. 17, 2023AvailabilityAcademic Institutions and Nonprofits only
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UG75-DGK1-3HA
Plasmid#184759PurposeDGK1 overexpression. Promotes the formation of phosphatidate from diacylglycerol. Uses antibiotic resistance marker hphMX6.DepositorAvailable sinceFeb. 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
IRE1-2EGFP-ter-kanMX4
Plasmid#184766PurposeIntegration of 2xGFP tag at IRE1 C terminus. Potential indicator of UPR activation. Uses antibiotic resistance marker kanMX4.DepositorAvailable sinceFeb. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
EMC1-2EGFP-ter-NAT
Plasmid#184763PurposeMarker for yeast endoplasmic reticulum (ER). Integration of 2xGFP tag at EMC1 C terminus. Uses antibiotic resistance marker natMX6.DepositorAvailable sinceFeb. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
trp1D-proLYS2-GFP-PKCdelta(C1a+C1b)-NAT
Plasmid#184774PurposeMarker for DAG. Yeast expression of mouse PKCdelta under LSY2 promoter. Uses antibiotic resistance marker natMX6. Replaces endogenous TRP1 upon genome integration, leading to trp1D.DepositorAvailable sinceFeb. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
UG76-pLYS2-GFP-PKCbeta
Plasmid#184762PurposeMarker for DAG. Yeast expression of human PKC beta under LSY2 promoter. Uses auxotrophic marker TRP1(Kluyveromyces lactis).DepositorAvailable sinceFeb. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
ABF2-2EGFP-ter-URA3
Plasmid#184768PurposeMarker for yeast mitochondria. Integration of 2xGFP tag at ABF2 C terminus. Uses auxotrophic marker URA3(Candida albicans).DepositorAvailable sinceFeb. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCEV-G2-Km ymScarletI
Plasmid#193960PurposeTEF1 promoter controlled expression plasmid with G418 resistance for expressing ymScarletI in budding yeastDepositorInsertymScarletI
ExpressionYeastPromoterTEF1Available sinceFeb. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
NAT-TET-OFF -MET - 3xHA
Plasmid#182526PurposeDoxycycline-induced transcriptional regulation and 3xHA N-terminal tagging.DepositorInsert7 Copies of TetO Box
Tags3HAExpressionYeastAvailable sinceJan. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
NAT-TET-OFF -LEU- 4xFLAG
Plasmid#182527PurposeDoxycycline-induced transcriptional regulation and Ubiquitin - LEU - 4xFLAG N-terminal tagging. Ubiquitin is cleaved off, resulting in an unstable protein with an N-terminal leucine.DepositorInsert7 Copies of TetO Box
Tags4XFLAGExpressionYeastAvailable sinceJan. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTPI1-EMC1-GFP-APEX2-URA3
Plasmid#184776PurposeExpresses EMC1-GFP-APEX2 chimera for labeling yeast endoplasmic reticulum (ER). Uses auxotrophic marker URA3(Kluyveromyces lactis).DepositorInsertAPEX2
ExpressionYeastAvailable sinceJan. 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
pILGFP4I
Plasmid#104504PurposeUsed to evaluate the expression output of Saccharomyces kudriavzevii GAL1 promoter with yEGFP used as the reporter geneDepositorInsertSkGAL1 promoter-yEGFP
ExpressionYeastAvailable sinceDec. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pILGFP4Q
Plasmid#104512PurposeUsed to evaluate the expression output of Saccharomyces kudriavzevii GAL2 promoter with yEGFP used as the reporter geneDepositorInsertSkGAL2 promoter-yEGFP
ExpressionYeastAvailable sinceDec. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAG25-1199 cDNA
Plasmid#160379PurposeN1199 narnavirus cDNADepositorInsertN1199
ExpressionYeastPromoterNAAvailable sinceDec. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pESC-NAT-Ago-Dcr
Plasmid#160381PurposeOverexpression of Ago-Dcr fusion protein in yeastDepositorInsertAgo- Dcr
ExpressionYeastPromoterGALAvailable sinceDec. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
N1199 launching
Plasmid#160386PurposeExpression of N1199 narnavirus ORF under PGK1 promoter with a 3' HDV ribozyme sequenceDepositorInsertN1199
ExpressionYeastPromoterPGK1Available sinceDec. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
Linked Free NES (M)
Plasmid#182436PurposeYeast integrative plasmid for expressing fusion protein ERG20(F96W-N127W)-AcNES1 (GAL10 promoter). Contains uracil auxotrophic marker (K. lactis URA3).DepositorPublicationInsertFPPS(M)-NES
UseSynthetic Biology; Metabolic engineeringExpressionYeastPromoterGAL10Available sinceDec. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
p414GPD-3xFLAG-Nop4 WT
Plasmid#169261PurposeS. cerevisiae (yeast) vector for constitutive expression of 3xFLAG-Nop4 WTDepositorAvailable sinceDec. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
Yeast_Target-AID3S
Plasmid#188646PurposeExpresses Target-AID3S in yeastsDepositorPublicationInsertnCas9-AID2S-UGI
TagsUGIExpressionYeastMutationD10A for SpCas9PromoterpGal1Available sinceNov. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pILGFP4L
Plasmid#104507PurposeUsed to evaluate the expression output of Saccharomyces mikatae GAL2 promoter with yEGFP used as the reporter geneDepositorInsertSmGAL2 promoter-yEGFP
ExpressionYeastAvailable sinceNov. 24, 2022AvailabilityAcademic Institutions and Nonprofits only