8,133 results

1. pCfB2228

   Plasmid

   #67540

   Purpose

   EasyClone system-based yeast integrative vector carrying loxP-flanked SpHIS5 marker, integration into S. cerevisiae XII-4 chromosomal location, USER site for cloning, amp resistance

   Depositor

   Irina Borodina

   Article

   Stovicek et al J Ind Microbiol Biotechnol. 2015 Sep 16.

   Type

   Empty backbone

   Use

   Cre/Lox; Integrative

   Expression

   Yeast

   Available Since

   March 10, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pPS1303

   Plasmid

   #8849

   Depositor

   Pamela Silver

   Article

   Shen et al Genes Dev 1998 Mar 1;12(5):679-91.

   Insert

   Gal-sGFP
   

   Expression

   Yeast

   Available Since

   Aug. 3, 2005

   Availability

   Academic Institutions and Nonprofits only

3. LD-BS-Trp1

   Plasmid

   #69503

   Purpose

   Improved version for complementation of yeast auxotrophic marker TRP1

   Depositor

   Zhiping Xie

   Article

   Li et al Autophagy. 2015;11(6):954-60. doi: 10.10

   Insert

   TRP1
   

   Expression

   Yeast

   Available Since

   Oct. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. p413GPD-hTPI Ile170Val

   Plasmid

   #50720

   Purpose

   expression of the human TPI Ile170Val allele in yeast

   Depositor

   Markus Ralser

   Article

   Gruning et al Open Biol. 2014 Mar 5;4(3):130232. doi:

   Insert

   Triosephosphate isomerase 1 (TPI1 Human)
   

   Expression

   Yeast

   Mutation

   Isoleucine 170 to Valine

   Promoter

   GPD

   Available Since

   Feb. 20, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pRS416 Gal-RNQ1 1-153-YFP

   Plasmid

   #18690

   Depositor

   Susan Lindquist

   Article

   Douglas et al Proc Natl Acad Sci U S A. 2008 May 20. 1

   Insert

   Rnq1 1-153
   

   Tags

   YFP

   Expression

   Yeast

   Mutation

   NonPrion domain of Rnq1

   Available Since

   June 11, 2008

   Availability

   Academic Institutions and Nonprofits only

6. Ube2v3

   Plasmid

   #31431

   Depositor

   Rachel Klevit

   Article

   Christensen et al Nat Struct Mol Biol. 2007 Oct . 14(10):9

   Insert

   Ubiquitin Conjugating Enzyme (UEVLD Human)
   

   Tags

   HA

   Expression

   Yeast

   Available Since

   Oct. 27, 2011

   Availability

   Academic Institutions and Nonprofits only

7. pMJ22

   Plasmid

   #63919

   Purpose

   Initial replicative vector

   Depositor

   Irene Castano Navarro

   Article

   Yanez-Carrillo et al Fungal Genet Biol. 2015 May 15. pii: S10

   Type

   Empty backbone

   Tags

   NATMX

   Expression

   Yeast

   Available Since

   July 6, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pGBW-m4046374

   Plasmid

   #149120

   Purpose

   Yeast Expression plasmid for SARS-CoV-2 3'-to-5' exonuclease

   Depositor

   Ginkgo Bioworks, Benjie Chen

   Insert

   SARS-CoV-2 3'-to-5' exonuclease (ORF1ab Severe acute respiratory syndrome coronavirus 2, Budding Yeast)
   

   Tags

   Cleavable thrombin;6xHIS

   Expression

   Yeast

   Mutation

   Saccharomyces cerevisiae recode 1

   Promoter

   pGAL1

   Available Since

   May 14, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

9. pGBW-m4046372

   Plasmid

   #149048

   Purpose

   Yeast Expression plasmid for SARS-CoV-2 helicase

   Depositor

   Ginkgo Bioworks, Benjie Chen

   Insert

   SARS-CoV-2 helicase (ORF1ab Severe acute respiratory syndrome coronavirus 2, Budding Yeast)
   

   Tags

   Cleavable thrombin;6xHIS

   Expression

   Yeast

   Mutation

   Saccharomyces cerevisiae recode 1

   Promoter

   pGAL1

   Available Since

   May 14, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

10. pGBW-m4046194

    Plasmid

    #148979

    Purpose

    Yeast Expression plasmid for SARS-CoV-2 ORF7a protein

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 ORF7a protein (ORF7a Severe acute respiratory syndrome coronavirus 2, Budding Yeast)
    

    Tags

    Cleavable thrombin;6xHIS

    Expression

    Yeast

    Mutation

    Saccharomyces cerevisiae recode 1

    Promoter

    pGAL1

    Available Since

    May 14, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

11. pGBW-m4046521

    Plasmid

    #145473

    Purpose

    Yeast Expression plasmid for SARS-CoV-2 nsp11

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 nsp11 (ORF1ab Severe acute respiratory syndrome coronavirus 2, Budding Yeast)
    

    Tags

    Cleavable thrombin;6xHIS

    Expression

    Yeast

    Mutation

    Saccharomyces cerevisiae recode 1

    Promoter

    pGAL1

    Available Since

    April 29, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

12. pNX3a-PK6

    Plasmid

    #78355

    Purpose

    fission yeast epitope tagging

    Depositor

    Kazunori Tomita

    Article

    Amelina et al BMC Biotechnol. 2016 Nov 8;16(1):76.

    Inserts

    6xPK
    kanMX6
    

    Expression

    Yeast

    Available Since

    Nov. 17, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pILGFP84

    Plasmid

    #83542

    Purpose

    Used to evaluate the expression output of SSA1 promoter with yEGFP used as the reporter gene.

    Depositor

    Claudia Vickers

    Article

    Peng et al Microb Cell Fact. 2015 Jun 26;14:91. doi

    Insert

    SSA1 promoter-yEGFP
    

    Expression

    Yeast

    Promoter

    SSA1

    Available Since

    Oct. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pGBW-m4046696

    Plasmid

    #153882

    Purpose

    Yeast expression plasmid for SARS-CoV-2 N (nucleocapsid)

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 N (nucleocapsid) (N Severe acute respiratory syndrome coronavirus 2)
    

    Expression

    Yeast

    Mutation

    wt

    Promoter

    pTEF1

    Available Since

    June 12, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

15. pSP571

    Plasmid

    #139498

    Purpose

    CRISPR-Cas9 plasmid to generate double strand break in STL1 locus in S. cerevisiae. Expresses both Cas9 and STL1 sgRNA

    Depositor

    Serge Pelet

    Article

    Wosika et al Nat Commun. 2020 Jun 23;11(1):3171. doi:

    Insert

    pGPD Cas9 / sgRNA (STL162)
    

    Expression

    Yeast

    Mutation

    WT

    Promoter

    pGPD

    Available Since

    June 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pTH399-SUP45-S30P

    Plasmid

    #29372

    Depositor

    Tobias von der Haar

    Article

    Merritt et al Nucleic Acids Res. 2010 Sep . 38(16):547

    Insert

    SUP45 gene encoding translation release factor 1 from S. cerevisiae (SUP45 Budding Yeast)
    

    Expression

    Yeast

    Mutation

    S30P mutant gene (corresponding to the sl23ts all…

    Available Since

    May 23, 2011

    Availability

    Academic Institutions and Nonprofits only

17. pTH375-SUP45-P174Q

    Plasmid

    #29383

    Depositor

    Tobias von der Haar

    Article

    Merritt et al Nucleic Acids Res. 2010 Sep . 38(16):547

    Insert

    SUP45 gene encoding translation release factor 1 from S. cerevisiae (SUP45 Budding Yeast)
    

    Expression

    Yeast

    Mutation

    P174Q mutant gene including genomic sequence 1432…

    Available Since

    May 23, 2011

    Availability

    Academic Institutions and Nonprofits only

18. pRS413-IVb

    Plasmid

    #31459

    Depositor

    Ana Kitazono

    Article

    Kitazono et al Gene. 2011 Sep 15;484(1-2):86-9. doi: 10

    Insert

    linker
    

    Expression

    Yeast

    Available Since

    Aug. 29, 2011

    Availability

    Academic Institutions and Nonprofits only

19. pTH734-CEN-minHIS3

    Plasmid

    #38227

    Depositor

    Tobias von der Haar

    Article

    Chu et al EMBO J. 2014 Jan 1;33(1):21-34. doi: 10.

    Insert

    HIS3 (HIS3 Synthetic, Budding Yeast)
    

    Tags

    HA

    Expression

    Yeast

    Mutation

    Every codon of the coding sequence has been repla…

    Promoter

    TDH3

    Available Since

    Dec. 19, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pTH736-CEN-staHIS3

    Plasmid

    #38228

    Depositor

    Tobias von der Haar

    Article

    Chu et al EMBO J. 2014 Jan 1;33(1):21-34. doi: 10.

    Insert

    HIS3 (HIS3 Synthetic, Budding Yeast)
    

    Tags

    HA

    Expression

    Yeast

    Promoter

    TDH3 (=GPD)

    Available Since

    Dec. 19, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge