8,128 results

1. pICE TRP1-URA3

   Plasmid

   #122894

   Purpose

   TRP1-URA3 ICE plasmid used to facilitate yeast recombination cloning and stable genomic Integration after CEN Excision.

   Depositor

   Randy Hampton

   Article

   Flagg et al Yeast. 2019 May 10. doi: 10.1002/yea.340

   Type

   Empty backbone

   Use

   Yeast recombination cloning

   Expression

   Yeast

   Available Since

   Feb. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pICE LYS2-HphMx

   Plasmid

   #122898

   Purpose

   LYS2-HphMx ICE plasmid used to facilitate yeast recombination cloning and stable genomic Integration after CEN Excision.

   Depositor

   Randy Hampton

   Article

   Flagg et al Yeast. 2019 May 10. doi: 10.1002/yea.340

   Type

   Empty backbone

   Use

   Yeast recombination cloning

   Expression

   Yeast

   Available Since

   Feb. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pICE LYS2-KanMx

   Plasmid

   #122899

   Purpose

   LYS2-KanMx ICE plasmid used to facilitate yeast recombination cloning and stable genomic Integration after CEN Excision.

   Depositor

   Randy Hampton

   Article

   Flagg et al Yeast. 2019 May 10. doi: 10.1002/yea.340

   Type

   Empty backbone

   Use

   Yeast recombination cloning

   Expression

   Yeast

   Available Since

   Feb. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pICE LYS2-NatMx

   Plasmid

   #122900

   Purpose

   LYS2-NatMx ICE plasmid used to facilitate yeast recombination cloning and stable genomic Integration after CEN Excision.

   Depositor

   Randy Hampton

   Article

   Flagg et al Yeast. 2019 May 10. doi: 10.1002/yea.340

   Type

   Empty backbone

   Use

   Yeast recombination cloning

   Expression

   Yeast

   Available Since

   Feb. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pICE TRP1-HphMx

   Plasmid

   #122901

   Purpose

   TRP1-HphMx ICE plasmid used to facilitate yeast recombination cloning and stable genomic Integration after CEN Excision.

   Depositor

   Randy Hampton

   Article

   Flagg et al Yeast. 2019 May 10. doi: 10.1002/yea.340

   Type

   Empty backbone

   Use

   Yeast recombination cloning

   Expression

   Yeast

   Available Since

   Feb. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. 6x_a1gRNAOp_pCYC1m_yeBFP2

   Plasmid

   #64392

   Purpose

   encodes six a1 operator sites upstream of minimal pCYC1 promoter driving yeast enhanced BFP

   Depositor

   Timothy Lu

   Article

   Farzadfard et al ACS Synth Biol. 2013 Sep 11.

   Insert

   yeast enhanced BFP2
   

   Expression

   Yeast

   Available Since

   May 12, 2015

   Availability

   Academic Institutions and Nonprofits only

7. pAG425GAL-ccdB-ECFP

   Plasmid

   #14177

   Depositor

   Susan Lindquist

   Article

   Alberti et al Yeast. 2007 Oct . 24(10):913-9.

   Type

   Empty backbone

   Use

   Gateway destination

   Tags

   ECFP

   Expression

   Yeast

   Available Since

   March 16, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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8. pTGPD-H-Hsp82

   Plasmid

   #1278

   Depositor

   Susan Lindquist

   Article

   Chang et al J Biol Chem 1994 Oct 7;269(40):24983-8.

   Insert

   Hsp82 (HSP82 Budding Yeast)
   

   Tags

   Histidinex6

   Expression

   Yeast

   Promoter

   GPD

   Available Since

   Jan. 6, 2005

   Availability

   Academic Institutions and Nonprofits only

9. pKdH_P-GAP-ScADH2+P-GAP*-ScALD6+ScACS1*

   Plasmid

   #126739

   Purpose

   yeast plasmid overexpressing ADH2,ALD6 and ACS1 from Saccharomyces cerevisiae

   Depositor

   Menghao Cai

   Article

   Liu et al Metab Eng. 2019 Jul;54:275-284. doi: 10.

   Insert

   adh2, acs1*, ald6
   

   Tags

   6xHis,Myc

   Expression

   Yeast

   Mutation

   T3542C and A30S, S629N (please see depositor comm…

   Promoter

   GAP

   Available Since

   July 31, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAG423GPD-ccdB-ECFP

    Plasmid

    #14174

    Depositor

    Susan Lindquist

    Article

    Alberti et al Yeast. 2007 Oct . 24(10):913-9.

    Type

    Empty backbone

    Use

    Gateway destination

    Tags

    ECFP

    Expression

    Yeast

    Available Since

    March 15, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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11. pNB763

    Plasmid

    #60158

    Purpose

    Firefly luciferase (Promega) driven by LYS9 promoter.

    Depositor

    Nicolas Buchler

    Article

    Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

    Insert

    FLuc
    

    Expression

    Yeast

    Mutation

    FLuc has A4V & S504G (no functional effect)

    Promoter

    LYS9pr

    Available Since

    March 25, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDUAL-P41nmt1-Nup124-GFP-MTn

    Plasmid

    #154365

    Purpose

    Yeast expression of Nup124

    Depositor

    Wanzhong He

    Article

    Jiang et al Nat Methods. 2020 Sep;17(9):937-946. doi

    Insert

    Nup124 (nup124 Fission Yeast)
    

    Expression

    Yeast

    Mutation

    See depositor comments below

    Available Since

    Aug. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. BB3aK_14*

    Plasmid

    #98529

    Purpose

    empty BB3 for insertion and overexpression of 1 gene in P. pastoris

    Depositor

    Brigitte Gasser, Diethard Mattanovich, Michael Sauer

    Article

    Prielhofer et al BMC Syst Biol. 2017 Dec 8;11(1):123. doi

    Type

    Empty backbone

    Expression

    Bacterial and Yeast

    Available Since

    Feb. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCH043

    Plasmid

    #47949

    Purpose

    rtTA under the control of GAL1 promoter

    Depositor

    Attila Becskei

    Article

    Hsu et al Nat Commun. 2012 Feb 21;3:682. doi: 10.1

    Insert

    rtTA
    

    Use

    Tet inducible expression

    Expression

    Yeast

    Promoter

    Gal1

    Available Since

    Nov. 14, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDH5

    Plasmid

    #83775

    Purpose

    Useful for tagging yeast proteins with C-terminal YFP tag; his selection

    Depositor

    Eric Muller

    Article

    Hailey et al Methods Enzymol. 2002;351:34-49.

    Insert

    YFP
    

    Tags

    YFP

    Expression

    Yeast

    Mutation

    S65G, V68L, Q69K, S72A, T203Y. Also includes Q80R…

    Available Since

    Oct. 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pRS414 Hsh155 WT

    Plasmid

    #87220

    Purpose

    Encodes Hsh155 WT for expression in yeast

    Depositor

    Aaron Hoskins

    Article

    Carrocci et al Nucleic Acids Res. 2017 Jan 6. pii: gkw1

    Insert

    HSH155 (HSH155 Budding Yeast)
    

    Expression

    Bacterial and Yeast

    Available Since

    July 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pGBW-m4046697

    Plasmid

    #153869

    Purpose

    Yeast expression plasmid for SARS-CoV-2 nsp4

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 nsp4 (ORF1ab Severe acute respiratory syndrome coronavirus 2)
    

    Expression

    Yeast

    Mutation

    wt

    Promoter

    pGAL1

    Available Since

    June 12, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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18. pGBW-m4046702

    Plasmid

    #149158

    Purpose

    Yeast Expression plasmid for SARS-CoV-2 nsp6

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 nsp6 (ORF1ab Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
    

    Expression

    Yeast

    Mutation

    wt

    Promoter

    pGAL1

    Available Since

    May 14, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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19. pGBW-m4046387

    Plasmid

    #149005

    Purpose

    Yeast Expression plasmid for SARS-CoV-2 nsp4

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 nsp4 (ORF1ab Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
    

    Tags

    Cleavable thrombin;StrepII

    Expression

    Yeast

    Mutation

    Saccharomyces cerevisiae recode 1

    Promoter

    pGAL1

    Available Since

    May 14, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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20. pNC952

    Plasmid

    #20401

    Depositor

    Beverly Errede

    Article

    Hackett et al Yeast. 2006 Apr 15. 23(5):333-49.

    Insert

    CFP-reporter
    

    Expression

    Yeast

    Mutation

    X=UbiM (see schematic) linker=deltak

    Available Since

    March 6, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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