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pFA NuiHA-loxP-nat-CUP1p

Plasmid

#131170

Purpose

PCR template for genomic N-terminal Nui-HA tag, nat-CUP1p flanking loxP sites allow expression by the native promoter after CRE-recombinase expression, nat marker confers resistance to Nourseothricin

Depositor

Nils Johnsson

Publication

Dunkler et al Methods Mol Biol. 2012;786:115-30. doi:

Type

Empty backbone

Tags

Nui-HA

Expression

Yeast

Available since

Sept. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pJW1671

Plasmid

#112045

Purpose

CEN/ARS plasmid for GAL-inducible ORM1-V5-TEV-EGFP expression marked with URA

Depositor

Jonathan Weissman

Publication

Costa et al Science. 2018 Jan 18. pii: science.aar36

Insert

ORM1

Tags

V5-TEV-EGFP

Expression

Yeast

Promoter

GAL1

Available since

Aug. 22, 2019

Availability

Academic Institutions and Nonprofits only

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pMV13

Plasmid

#128864

Purpose

Position 1 module- CEN (Centromeric)

Depositor

Gilles Charvin

Publication

Goulev et al PLoS One. 2019 Aug 28;14(8):e0220694. do

Insert

CEN/ARS

Use

Synthetic Biology

Expression

Yeast

Available since

Aug. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pMJA053

Plasmid

#128515

Purpose

Expresses SFA8 in Pichia

Depositor

Marcos Alcocer

Publication

Alcocer et al J Mol Biol. 2002 Nov 15;324(1):165-75.

Insert

SFA8 a 2S albumin from sunflower (LOC110892047 Helianthus annus)

Expression

Yeast

Promoter

AOX1

Available since

Aug. 9, 2019

Availability

Academic Institutions and Nonprofits only

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pMV12

Plasmid

#128893

Purpose

Position 6 module- NatMX

Depositor

Gilles Charvin

Publication

Goulev et al PLoS One. 2019 Aug 28;14(8):e0220694. do

Insert

NatMX

Use

Synthetic Biology

Expression

Yeast

Available since

Aug. 9, 2019

Availability

Academic Institutions and Nonprofits only

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pKdH_P-GAP-ScADH2+P-GAP*-ScALD6+ScACS1*

Plasmid

#126739

Purpose

yeast plasmid overexpressing ADH2,ALD6 and ACS1 from Saccharomyces cerevisiae

Depositor

Menghao Cai

Publication

Liu et al Metab Eng. 2019 Jul;54:275-284. doi: 10.

Insert

adh2, acs1*, ald6

Tags

6xHis,Myc

Expression

Yeast

Mutation

T3542C and A30S, S629N (please see depositor comm…

Promoter

GAP

Available since

July 31, 2019

Availability

Academic Institutions and Nonprofits only

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Designed VEGF-binding scFv G6des1

Plasmid

#110212

Purpose

Yeast Surface Display of G6des1

Depositor

Sarel Fleishman

Publication

Warszawski et al PLoS Comput Biol. 2019 Aug 23;15(8):e100

Insert

G6des1

Tags

cmyc tag

Expression

Yeast

Mutation

L A43P, L Y49F, L Y55F ,L Q89L, L T94V, L P95T, L…

Available since

July 25, 2019

Availability

Academic Institutions and Nonprofits only

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pMJA151

Plasmid

#128526

Purpose

Expresses mutant Ber e 1 in Pichia

Depositor

Marcos Alcocer

Insert

mutant Ber e 1

Expression

Yeast

Promoter

AOX1

Available since

July 24, 2019

Availability

Academic Institutions and Nonprofits only

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HEL binding scFv D44.1

Plasmid

#111718

Purpose

Yeast Surface Display of D44.1 as a reference starting point for design

Depositor

Sarel Fleishman

Publication

Warszawski et al PLoS Comput Biol. 2019 Aug 23;15(8):e100

Insert

D44.1

Tags

cmyc tag

Expression

Yeast

Available since

July 22, 2019

Availability

Academic Institutions and Nonprofits only

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pZ_lacO-cP-AOX1-CPR

Plasmid

#126742

Purpose

yeast plasmid overexpressing CPR

Depositor

Menghao Cai

Publication

Liu et al Metab Eng. 2019 Jul;54:275-284. doi: 10.

Insert

cpr

Tags

6xHis

Expression

Yeast

Available since

July 10, 2019

Availability

Academic Institutions and Nonprofits only

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pZ_lacO-cP-AOX1-eGFP

Plasmid

#126720

Purpose

yeast plasmid overexpressing eGFP

Depositor

Menghao Cai

Publication

Liu et al Metab Eng. 2019 Jul;54:275-284. doi: 10.

Insert

egfp

Tags

6xHis,Myc

Expression

Yeast

Available since

June 28, 2019

Availability

Academic Institutions and Nonprofits only

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pZ_P-GAP-LacI-Mit1AD

Plasmid

#126722

Purpose

LacI-Mit1AD on pGAPZ_ A expression vector

Depositor

Menghao Cai

Publication

Liu et al Metab Eng. 2019 Jul;54:275-284. doi: 10.

Insert

hybrid lacI-Mit1 activation domain coding sequence

Expression

Yeast

Promoter

GAP

Available since

June 28, 2019

Availability

Academic Institutions and Nonprofits only

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pZ_lacO-cP-AOX1-sLovA

Plasmid

#126741

Purpose

yeast plasmid overexpressing sLovA

Depositor

Menghao Cai

Publication

Liu et al Metab Eng. 2019 Jul;54:275-284. doi: 10.

Insert

slovA from Aspergillus terreus ATCC 20542 (LovA) codon optimized for expression in Komagataella phaffii

Tags

6xHis

Expression

Yeast

Available since

June 28, 2019

Availability

Academic Institutions and Nonprofits only

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pUC_delta ald1

Plasmid

#126708

Purpose

vector for knocking out ald1 in Komagataella phaffii

Depositor

Menghao Cai

Publication

Liu et al Metab Eng. 2019 Jul;54:275-284. doi: 10.

Insert

HIS4

Expression

Yeast

Promoter

His

Available since

June 28, 2019

Availability

Academic Institutions and Nonprofits only

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pZ_P-GAP-ACS1

Plasmid

#126712

Purpose

yeast plasmid complementing ACS1 from Komagataella phaffii

Depositor

Menghao Cai

Publication

Liu et al Metab Eng. 2019 Jul;54:275-284. doi: 10.

Insert

acs1

Tags

6xHis,Myc

Expression

Yeast

Promoter

GAP

Available since

June 19, 2019

Availability

Academic Institutions and Nonprofits only

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pDRF1-GW yosfGFP-T2A-mCherry

Plasmid

#118448

Purpose

Produces equimolar expression of yeast codon-optimized sfGFP and mCherry

Depositor

Bas Teusink

Publication

Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

Insert

yosfGFP-T2A-mCherry

Expression

Yeast

Available since

June 10, 2019

Availability

Academic Institutions and Nonprofits only

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pDRF1-GW eGFP-T2A-mCherry

Plasmid

#118422

Purpose

Produces equimolar expression of eGFP and mCherry

Depositor

Bas Teusink

Publication

Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

Insert

eGFP-T2A-mCherry

Expression

Yeast

Available since

June 3, 2019

Availability

Academic Institutions and Nonprofits only

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pCfB4613

Plasmid

#126752

Purpose

Integrate pTRX2_5xUAS-CDC14-tADH1 into yeast genome EasyClone site X-4 with SpHIS5 marker

Depositor

Michael Jensen

Publication

Zhang et al ACS Synth Biol. 2016 Dec 16;5(12):1546-1

Insert

CDC14 (CDC14 Budding Yeast)

Use

Integrative plasmid

Expression

Yeast

Promoter

pTRX2_5xUAS

Available since

June 3, 2019

Availability

Academic Institutions and Nonprofits only

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pMST-1312

Plasmid

#118075

Purpose

pTPI1-cexA-tCYC1

Depositor

Michael Sauer

Publication

Steiger et al Metab Eng. 2019 Mar;52:224-231. doi: 10.

Insert

pTPI1-cexA-tCYC1

Expression

Yeast

Promoter

pTPI1

Available since

May 20, 2019

Availability

Academic Institutions and Nonprofits only

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wt Cus2p

Plasmid

#73046

Purpose

Wild-type S. cerevisiae Cus2p gene for recombinant expression and crystallization

Depositor

Aaron Hoskins

Publication

Rodgers et al RNA. 2015 Dec 2.

Insert

CUS2p (CUS2 Budding Yeast)

Tags

6x His tag fused to TEV cleavage site

Expression

Bacterial and Yeast

Promoter

T7

Available since

May 14, 2019

Availability

Academic Institutions and Nonprofits only

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