8,430 results
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Plasmid#63656PurposepCfB2801 is a vector for multiple integrations at sites sharing homology with Ty1Cons2. The selective marker is Kl.LEU2. Additionally it contains a PTEF1-GFP as reporter.DepositorInsertGFP
UseCre/LoxExpressionYeastPromoterTEF1 promoterAvailable sinceAug. 15, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pESC-NAT-LACap
Plasmid#80763PurposeExpresses truncated L-A capsid for curing satellite virusDepositorInsertL-A cDNA (L-A Cap)
ExpressionYeastPromoterPGK1Available sinceAug. 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDZ529 pTRP MET25p PP7-PS-2x-yeGFP
Plasmid#72233PurposeTryptophan selection; Expression of PP7-2x-yeGFP fusion protein under the MET25 promoterDepositorInsertPP7-PS-2x-yeGFP
ExpressionYeastPromoterMET25Available sinceAug. 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pWHI2-NAT
Plasmid#80588PurposeCEN plasmid expressing full-length WHI2 from S.cerevisiae S288C background under its native promoterDepositorAvailable sinceJuly 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
pREP3xN_ccdB
Plasmid#79021PurposeGateway destination vector for S. pombe. Allow N-terminal tag(s) to be introduced using MCS. attR1-CmR-ccdB-attR2 cassette, nmt1 (ATATATAAA) promoter, LEU2 selectionDepositorTypeEmpty backboneExpressionYeastAvailable sinceJuly 7, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pREP82xN_ccdB
Plasmid#79026PurposeGateway destination vector for S. pombe. Allow N-terminal tag(s) to be introduced using MCS. attR1-CmR-ccdB-attR2 cassette, nmt1** (AT) promoter, ura4+ selectionDepositorTypeEmpty backboneExpressionYeastAvailable sinceJuly 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pREP81xN_ccdB
Plasmid#79025PurposeGateway destination vector for S. pombe. Allow N-terminal tag(s) to be introduced using MCS. attR1-CmR-ccdB-attR2 cassette, nmt1** (AT) promoter, LEU2 selectionDepositorTypeEmpty backboneExpressionYeastAvailable sinceJuly 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pREP4xV5_ccdB
Plasmid#79016PurposeGateway destination vector for S. pombe. Allow sequence of interest to be fused to N-terminal double V5 epitope tag. attR1-CmR-ccdB-attR2 cassette, nmt1 (ATATATAAA) promoter, ura4+ selectionDepositorTypeEmpty backboneTagsdouble V5 epitope tagExpressionYeastAvailable sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pIM35
Plasmid#74644PurposeCentromeric plasmid to construct episomal C-terminal fusions of genes with yeast-adapted TagRFP-T and URA3 selection markerDepositorInsertyTagRFP-T
ExpressionYeastPromoterMET25Available sinceJune 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK276
Plasmid#73366PurposeEncodes Crepis alpina plastid omega-3 fatty acid desaturase (FAD7)DepositorInsertplastid omega-3 fatty acid desaturase
ExpressionYeastMutationMutation I231F. Also insert is missing residues 1…PromoterGAL1Available sinceMay 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCfB2374
Plasmid#67534PurposeEasyClone system-based yeast integrative vector carrying loxP-flanked KlURA3 marker, integration into S. cerevisiae XI-1 chromosomal location, USER site for cloning, amp resistanceDepositorTypeEmpty backboneUseCre/Lox; IntegrativeExpressionYeastAvailable sinceMarch 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK371
Plasmid#73596PurposeEncodes Crepis alpina delta-12 fatty acid acetylenase (vFAD2) with C-terminal FLAG epitope and delta-12 fatty acid desaturase (FAD2-3) with C-terminal c-myc epitopeDepositorInsertsdelta-12 fatty acid acetylenase
delta-12 fatty acid desaturase (form 3)
TagsFLAG tag and c-myc tagExpressionYeastMutationPoint mutants in FAD2-3 ORF relative to deposited…PromoterGAL1 and GAL10Available sinceMarch 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK142
Plasmid#73317PurposeEncodes Crepis alpina delta-12 fatty acid acetylenase (vFAD2) as a fusion to Pas2p, with c-myc tag and His6-tagDepositorInsertdelta-12 fatty acid acetylenase
TagsHis6, Pas2p, and c-mycExpressionYeastMutationsynthetic gene. Encodes a glycine-204 to serine m…PromoterAOX1Available sinceFeb. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK694
Plasmid#72379PurposeProduces human phosphoribosyl pyrophosphate amidotransferase (PRAT), synthetic geneDepositorAvailable sinceFeb. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTG005
Plasmid#71386Purposelocalization of yeast RNase RMP1 (RNase MRP binding protein) in yeastDepositorAvailable sinceFeb. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK277
Plasmid#71565PurposeProduces Crepis alpina delta15 fatty acid desaturase (FAD3) in yeastDepositorInsertdelta15 fatty acid desaturase
ExpressionBacterial and YeastPromoterGAL1Available sinceJan. 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK273
Plasmid#71563PurposeProduces Crepis alpina delta12 fatty acid desaturase (FAD2-2) in yeastDepositorInsertdelta12 fatty acid desaturase (form 2)
ExpressionBacterial and YeastPromoterGAL1Available sinceJan. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDS1U-PKS12
Plasmid#70150PurposeFusarium graminearum PKS12 encoded on CEN/ARS yeast shuttle vector with DS1U selection markerDepositorAvailable sinceDec. 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDRFLIP42
Plasmid#65518PurposeFRET biosensor Destination vector for yeast expression. Encodes a FRET pair for fusion with ligand binding domain of interest.DepositorTypeEmpty backboneTags6His-Cit-attR1 and attR2-mCer-cMycExpressionYeastPromoterpPMA1Available sinceDec. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDRFLIP35
Plasmid#65513PurposeFRET biosensor Destination vector for yeast expression. Encodes a FRET pair for fusion with ligand binding domain of interest.DepositorTypeEmpty backboneTags6His-AFPt9-attR1 and attR2-TFPt9-cMycExpressionYeastPromoterpPMA1Available sinceNov. 13, 2015AvailabilityAcademic Institutions and Nonprofits only