8,430 results

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BS-Lys2

Plasmid

#69199

Purpose

complementation of yeast auxotrophic marker LYS2

Depositor

Zhiping Xie

Publication

Li et al Autophagy. 2015;11(6):954-60. doi: 10.10

Insert

LYS2

Expression

Yeast

Available since

Nov. 3, 2015

Availability

Academic Institutions and Nonprofits only

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LD121

Plasmid

#69172

Purpose

Integration of GFP tag at Atg21 C terminus, use auxotrophic marker URA3(Candida albicans)

Depositor

Zhiping Xie

Publication

Li et al Autophagy. 2015;11(6):954-60. doi: 10.10

Insert

Atg21-EGFP

Tags

EGFP

Expression

Yeast

Available since

Oct. 28, 2015

Availability

Academic Institutions and Nonprofits only

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LD111

Plasmid

#69165

Purpose

Integration of GFP tag at Atg11 C terminus, use auxotrophic marker URA3(Candida albicans)

Depositor

Zhiping Xie

Publication

Li et al Autophagy. 2015;11(6):954-60. doi: 10.10

Insert

Atg11-EGFP

Tags

EGFP

Expression

Yeast

Available since

Oct. 28, 2015

Availability

Academic Institutions and Nonprofits only

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LD106

Plasmid

#69163

Purpose

Integration of GFP tag at Atg6 C terminus, use auxotrophic marker URA3(Candida albicans)

Depositor

Zhiping Xie

Publication

Li et al Autophagy. 2015;11(6):954-60. doi: 10.10

Insert

Atg6-EGFP

Tags

EGFP

Expression

Yeast

Available since

Oct. 28, 2015

Availability

Academic Institutions and Nonprofits only

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pED45: pRS306 pRPS2-Ste7DS-NLS-mCherry

Plasmid

#68274

Purpose

SKARS: Fus3 and Kss1 MAPK activity sensor: Ste7DS 2xNLS mCherry

Depositor

Serge Pelet

Publication

Durandau et al BMC Biol. 2015 Aug 1;13:55. doi: 10.1186

Insert

Ste7DS NLS mCherry

Tags

mCherry

Expression

Yeast

Promoter

pRPS2

Available since

Sept. 14, 2015

Availability

Academic Institutions and Nonprofits only

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LD117

Plasmid

#69169

Purpose

Integration of GFP tag at Atg17 C terminus, use auxotrophic marker URA3(Candida albicans)

Depositor

Zhiping Xie

Publication

Li et al Autophagy. 2015;11(6):954-60. doi: 10.10

Insert

Atg17-EGFP

Tags

EGFP

Expression

Yeast

Available since

Sept. 14, 2015

Availability

Academic Institutions and Nonprofits only

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pRG227

Plasmid

#64532

Purpose

Episomal E. coli/S. cerevisiae shuttle vector

Depositor

Joerg Stelling

Publication

Gnugge et al Yeast. 2015 Dec 9. doi: 10.1002/yea.3144

Type

Empty backbone

Expression

Yeast

Available since

Aug. 28, 2015

Availability

Academic Institutions and Nonprofits only

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pDRFLIP32

Plasmid

#65511

Purpose

FRET biosensor Destination vector for yeast expression. Encodes a FRET pair for fusion with ligand binding domain of interest.

Depositor

Wolf Frommer

Publication

Jones et al eLife. 2014 Apr 15;3:e01741. doi: 10.755

Type

Empty backbone

Tags

6His-AFPt9-attR1 and attR2-t7CFPt9-cMyc

Expression

Yeast

Promoter

pPMA1

Available since

June 18, 2015

Availability

Academic Institutions and Nonprofits only

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PSUC2-GFPantisense-CYC1t-pRS413

Plasmid

#64404

Purpose

SUC2 regulated expression of an antisense RNAi construct for GFP

Depositor

Claudia Vickers

Publication

Williams et al Microb Cell Fact. 2015 Apr 1;14:43. doi:

Insert

GFPantisense

Expression

Yeast

Promoter

SUC2

Available since

June 11, 2015

Availability

Academic Institutions and Nonprofits only

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pILGFP3

Plasmid

#65451

Purpose

pILGFP3 was designed to test the promoter activity using the yeast enhanced green fluorescent protein (yEGFP) as reporter.

Depositor

Claudia Vickers

Publication

Peng et al Microb Cell Fact. 2015 Jun 26;14:91. doi

Type

Empty backbone

Tags

yEGFP reporter

Expression

Yeast

Available since

June 9, 2015

Availability

Academic Institutions and Nonprofits only

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pITGFP3

Plasmid

#65452

Purpose

pITGFP3 was designed to test the promoter activity using the destabilized yeast enhanced green fluorescent protein (yEGFP-CLN2PEST) as reporter.

Depositor

Claudia Vickers

Publication

Peng et al Microb Cell Fact. 2015 Jun 26;14:91. doi

Type

Empty backbone

Tags

yEGFP-CLN2PEST reporter

Expression

Yeast

Available since

June 2, 2015

Availability

Academic Institutions and Nonprofits only
3x_a1gRNAOp_pCYC1m_yeBFP2

Plasmid

#64391

Purpose

encodes three a1 operator sites upstream of minimal pCYC1 promoter driving yeast enhanced BFP

Depositor

Timothy Lu

Publication

Farzadfard et al ACS Synth Biol. 2013 Sep 11.

Insert

yeast enhanced BFP2

Expression

Yeast

Available since

May 12, 2015

Availability

Academic Institutions and Nonprofits only
pRS424-TUB2 - human TUBB6 Cterminal tail

Plasmid

#60403

Purpose

Expression of chimeric yeast beta tubulin (TUB2) - human beta5 tubulin Cterminal tail (TUBB6) using a GAL promoter

Depositor

Ron Vale

Publication

Sirajuddin et al Nat Cell Biol. 2014 Apr;16(4):335-44. do

Insert

TUB2

Expression

Yeast

Mutation

TUB2 amino acids 429 - end, replaced with human T…

Promoter

GAL

Available since

May 6, 2015

Availability

Academic Institutions and Nonprofits only
pRPR1_c6gRNA_RPR1t

Plasmid

#64384

Purpose

encodes c6 gRNA

Depositor

Timothy Lu

Publication

Farzadfard et al ACS Synth Biol. 2013 Sep 11.

Insert

c6 gRNA

Expression

Yeast

Available since

May 5, 2015

Availability

Academic Institutions and Nonprofits only
pRPR1_a1gRNA_RPR1t

Plasmid

#64378

Purpose

encodes a1 gRNA

Depositor

Timothy Lu

Publication

Farzadfard et al ACS Synth Biol. 2013 Sep 11.

Insert

a1 gRNA

Expression

Yeast

Available since

May 5, 2015

Availability

Academic Institutions and Nonprofits only
pYC16

Plasmid

#63913

Purpose

Recipient overexpressing vector to construct CFP tagged fusions

Depositor

Irene Castano Navarro

Publication

Yanez-Carrillo et al Fungal Genet Biol. 2015 May 15. pii: S10

Type

Empty backbone

Tags

CFP

Expression

Yeast

Promoter

TEF1 (Saccharomyces cerevisiae)

Available since

April 27, 2015

Availability

Academic Institutions and Nonprofits only

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pJW1508

Plasmid

#62362

Purpose

pFA6a BirA-mVenus-SSH1::HIS5

Depositor

Jonathan Weissman

Publication

Jan et al Science. 2014 Nov 7;346(6210):1257521. d

Insert

BirA-mVenus-Ssh1

Tags

BirA-mVenus

Expression

Yeast

Promoter

Gal1

Available since

April 21, 2015

Availability

Academic Institutions and Nonprofits only

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pJW1516

Plasmid

#62386

Purpose

pFA6a Osm1(M1A)-yoEGFP::LEU2

Depositor

Jonathan Weissman

Publication

Williams et al Science. 2014 Nov 7;346(6210):748-51. do

Insert

OSM1-EGFP fusion

Tags

EGFP

Expression

Yeast

Mutation

Methionine 1 to Alanine

Promoter

Adh1p

Available since

April 2, 2015

Availability

Academic Institutions and Nonprofits only

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pNB785

Plasmid

#60164

Purpose

Firefly luciferase (Promega) yeast codon-optimized yellow fluorescent protein (yEVenus) fusion with PEST degron driven by SIC1 promoter.

Depositor

Nicolas Buchler

Publication

Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

Insert

FLuc-yEVenus-PEST

Tags

PEST degron

Expression

Yeast

Mutation

FLuc has A4V & S504G (no functional effect)

Promoter

SIC1pr

Available since

March 25, 2015

Availability

Academic Institutions and Nonprofits only

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pNB761

Plasmid

#60156

Purpose

Firefly luciferase (Promega) driven by ADE17 promoter.

Depositor

Nicolas Buchler

Publication

Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

Insert

FLuc

Expression

Yeast

Mutation

FLuc has A4V & S504G (no functional effect)

Promoter

ADE17pr

Available since

March 25, 2015

Availability

Academic Institutions and Nonprofits only

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