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K218Tc-3

Plasmid

#1300

Depositor

Susan Lindquist

Article

Schirmer et al Proc Natl Acad Sci U S A 2001 Jan 30;98(

Insert

mutant HSP104 (HSP104 Budding Yeast)

Expression

Yeast

Mutation

K218T: A315T

Available Since

Jan. 6, 2005

Availability

Academic Institutions and Nonprofits only
pAG305GAL-Cerulean-ccdB

Plasmid

#14401

Depositor

Susan Lindquist

Article

Alberti et al Yeast. 2007 Oct . 24(10):913-9.

Type

Empty backbone

Use

Gateway destination

Tags

Cerulean

Expression

Yeast

Available Since

June 18, 2007

Availability

Academic Institutions and Nonprofits only

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pLEP1Hsp82NdeI

Plasmid

#1276

Depositor

Susan Lindquist

Article

Chang et al J Biol Chem 1994 Oct 7;269(40):24983-8.

Insert

Hsp82 (HSP82 N/A)

Expression

Yeast

Available Since

Jan. 6, 2005

Availability

Academic Institutions and Nonprofits only
pKAT6

Plasmid

#1215

Depositor

Susan Lindquist

Article

Nathan et al Mol Cell Biol 1995 Jul;15(7):3917-25.

Insert

Hsc82 (HSC82 Budding Yeast)

Use

2 micron

Expression

Yeast

Mutation

vector backbone is YEp24 URA3 selection

Available Since

July 18, 2005

Availability

Academic Institutions and Nonprofits only
pRS316-pTEF-Sc_IntAct-tCYC

Plasmid

#248980

Purpose

Low-copy centromeric plasmids which expresses Saccharomyces cerevisiae IntAct (actin internally tagged with ALFA tag between T229 and A230 amino acids)

Depositor

Saravanan Palani

Article

van Zwam et al PLoS Biol. 2024 Mar 11;22(3):e3002551. d

Insert

Sc_IntAct

Tags

ALFA tag

Expression

Yeast

Promoter

TEF

Available Since

March 11, 2026

Availability

Academic Institutions and Nonprofits only

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pDUAL-pAct1-Sp_IntAct-tADH1

Plasmid

#250862

Purpose

Low-copy centromeric plasmids which expresses Schizosaccharomyces pombe IntAct (actin internally tagged with ALFA tag between T229 and A230 amino acids)

Depositor

Saravanan Palani

Article

van Zwam et al PLoS Biol. 2024 Mar 11;22(3):e3002551. d

Insert

Sp_IntAct

Tags

ALFA tag

Expression

Yeast

Promoter

TEF

Available Since

March 11, 2026

Availability

Academic Institutions and Nonprofits only

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pPT24-mtArg8m*-V5-UTR-HH-sgRNA_ATP6-HDV

Plasmid

#251981

Purpose

For biolistic transformation to express Arg8m* and control sgRNA targeting ATP6 from mtDNA

Depositor

Alice Ting

Article

Yin et al Proc Natl Acad Sci U S A. 2026 Jan 20;12

Inserts

mtArg8m*
HH-sgRNA_ATP6-HDV

Tags

V5

Expression

Yeast

Mutation

premature stop CAA->TAA, Q79*

Available Since

March 4, 2026

Availability

Academic Institutions and Nonprofits only

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pVG173

Plasmid

#251208

Purpose

Expresses a version of light-inducible transcription factor EL222 (Gly80Arg) under a strong constitutive PGK1pr

Depositor

Sahand Jamal Rahi

Article

Gligorovski et al Cell. 2026 Mar 6:S0092-8674(26)00167-4.

Insert

EL222 codon-optimized for budding yeast

Use

Synthetic Biology

Expression

Bacterial and Yeast

Mutation

Gly80Arg

Promoter

PGK1pr

Available Since

March 3, 2026

Availability

Academic Institutions and Nonprofits only

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pVG298

Plasmid

#227762

Purpose

Expresses a version of light-inducible transcription factor EL222 (Thr83Ala) under a strong constitutive PGK1pr

Depositor

Sahand Jamal Rahi

Article

Gligorovski et al Cell. 2026 Mar 6:S0092-8674(26)00167-4.

Insert

EL222 codon-optimized for budding yeast

Expression

Yeast

Mutation

Thr83Ala mutant

Promoter

PGK1pr

Available Since

March 3, 2026

Availability

Academic Institutions and Nonprofits only

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pADH1-MTS-ABE8e-P2A-EGFP

Plasmid

#251973

Purpose

Yeast mtDNA editing with a mid-strength promoter for lower expression level

Depositor

Alice Ting

Article

Yin et al Proc Natl Acad Sci U S A. 2026 Jan 20;12

Insert

ABE8e

Use

CRISPR

Tags

MTS and V5-P2A-EGFP

Expression

Yeast

Promoter

ADH1

Available Since

Feb. 24, 2026

Availability

Academic Institutions and Nonprofits only

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pPT24-mtArg8m-V5

Plasmid

#251979

Purpose

For biolistic transformation to express Arg8m from mtDNA

Depositor

Alice Ting

Article

Yin et al Proc Natl Acad Sci U S A. 2026 Jan 20;12

Insert

mtArg8m

Tags

V5

Expression

Yeast

Available Since

Feb. 24, 2026

Availability

Academic Institutions and Nonprofits only

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MTS-ABE-ARG8

Plasmid

#251976

Purpose

Expression of ABE-ARG8 fusion protein in yeast

Depositor

Alice Ting

Article

Yin et al Proc Natl Acad Sci U S A. 2026 Jan 20;12

Insert

ABE-ARG8

Use

CRISPR

Tags

MTS

Expression

Yeast

Promoter

GPD

Available Since

Feb. 24, 2026

Availability

Academic Institutions and Nonprofits only

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NLS-ABE8e-GFP

Plasmid

#251972

Purpose

Yeast nDNA editing with ABE8e

Depositor

Alice Ting

Article

Yin et al Proc Natl Acad Sci U S A. 2026 Jan 20;12

Insert

ABE8e

Use

CRISPR

Tags

3xNLS-3xFLAG-EGFP and NLS

Expression

Yeast

Promoter

GPD

Available Since

Feb. 23, 2026

Availability

Academic Institutions and Nonprofits only

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pPT24-mtArg8m*-V5

Plasmid

#251978

Purpose

For biolistic transformation to express Arg8m* from mtDNA

Depositor

Alice Ting

Article

Yin et al Proc Natl Acad Sci U S A. 2026 Jan 20;12

Insert

mtArg8m*

Tags

V5

Expression

Yeast

Mutation

premature stop CAA->TAA, Q79*

Available Since

Feb. 23, 2026

Availability

Academic Institutions and Nonprofits only

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pRS426-SNR52-crRNA

Plasmid

#251984

Purpose

Expression of crRNA (version 3) for editing, used together with tracrRNA Plasmid #251985. This plasmid is also called crRNA in the paper.

Depositor

Alice Ting

Article

Yin et al Proc Natl Acad Sci U S A. 2026 Jan 20;12

Insert

crRNA

Use

CRISPR

Expression

Yeast

Promoter

SNR52

Available Since

Feb. 23, 2026

Availability

Academic Institutions and Nonprofits only

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pPT24-mtArg8m*-V5-UTR-HH-ntsgRNA-HDV

Plasmid

#251982

Purpose

For biolistic transformation to express Arg8m* and a non-targeting control sgRNA from mtDNA

Depositor

Alice Ting

Article

Yin et al Proc Natl Acad Sci U S A. 2026 Jan 20;12

Inserts

mtArgm*
HH-nt sgRNA-HDV

Tags

V5

Expression

Yeast

Mutation

premature stop CAA->TAA, Q79*

Available Since

Feb. 23, 2026

Availability

Academic Institutions and Nonprofits only

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nArg8-EGFP

Plasmid

#251977

Purpose

Expression of nuclear ARG8-EGFP as control

Depositor

Alice Ting

Article

Yin et al Proc Natl Acad Sci U S A. 2026 Jan 20;12

Insert

nArg8-EGFP

Tags

EGFP

Expression

Yeast

Promoter

GPD

Available Since

Feb. 23, 2026

Availability

Academic Institutions and Nonprofits only

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pRembiv

Plasmid

#252216

Purpose

Yeast expression of BK polyomavirus VP1 and aeBlue reporter

Depositor

Christopher Buck

Inserts

BKV-IV VP1
aeBlue
Formaldehyde dehydrogenase 1

Expression

Yeast

Promoter

FDH1 and MAL32

Available Since

Feb. 23, 2026

Availability

Industry, Academic Institutions, and Nonprofits

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pV1210

Plasmid

#242891

Purpose

Modified plasmid from pV1200 to perform ENO1-SI CRISPR in Candidozyma auris

Depositor

Patrick Van Dijck

Article

Sofras et al bioRxiv [Preprint]. 2025 Jan 11:2025.01.

Insert

CaCas9/sgRNA-BsmBI stuffer

Use

CRISPR

Expression

Yeast

Available Since

Feb. 19, 2026

Availability

Academic Institutions and Nonprofits only

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HRPEadh-pAG303

Plasmid

#249735

Purpose

Derived from pAG303GPD-ccdb (#14134); GPD replaced with a synthetic 5xHRPE-ADHutr sequence for orthogonal activation by plant RAP2.12-based TFs.

Depositor

Francesco Licausi

Article

Lavilla-Puerta et al bioRxiv 2025.09.01.673502

Type

Empty backbone

Expression

Yeast

Available Since

Feb. 9, 2026

Availability

Academic Institutions and Nonprofits only

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