8,126 results

1. pAG415Gal_Gcn4 ILVtoED WGFP

   Plasmid

   #233007

   Purpose

   Galactose iduced expression of Gcn4 LVtoED W+GFPin yeast

   Depositor

   Tanja Mittag, Aseem Ansari

   Article

   Bremer et al bioRxiv 2024.11.21.624739

   Insert

   Gcn4 ILVtoED W+
   

   Tags

   HA-GFP-HA and TEV cleavage site

   Expression

   Yeast

   Mutation

   N126W, I128E, V130E, V135E, L137D, I142D

   Promoter

   Gal1

   Available Since

   March 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAG415Gal_Gcn4 R3W

   Plasmid

   #232975

   Purpose

   Galactose iduced expression of Gcn4 R++ W+ in yeast

   Depositor

   Tanja Mittag, Aseem Ansari

   Article

   Bremer et al bioRxiv 2024.11.21.624739

   Insert

   Gcn4 R++ W+
   

   Tags

   3xHA and TEV cleavage site

   Expression

   Yeast

   Mutation

   D103R, N126W, D133R, K143R

   Promoter

   Gal1

   Available Since

   March 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pAG415Gal_Gcn4 WT 44mer

   Plasmid

   #232956

   Purpose

   Galactose iduced expression of Gcn4 WT 44mer in yeast

   Depositor

   Tanja Mittag, Aseem Ansari

   Article

   Bremer et al bioRxiv 2024.11.21.624739

   Insert

   Gcn4 WT 44mer
   

   Tags

   TEV cleavage site

   Expression

   Yeast

   Promoter

   Gal1

   Available Since

   March 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pAG415Gal_Gcn4 E3HA

   Plasmid

   #232996

   Purpose

   Galactose iduced expression of Gcn4 E+HA in yeast

   Depositor

   Tanja Mittag, Aseem Ansari

   Article

   Bremer et al bioRxiv 2024.11.21.624739

   Insert

   Gcn4 E+
   

   Tags

   3xHA and TEV cleavage site

   Expression

   Yeast

   Mutation

   D105E, D118E, D139E

   Promoter

   Gal1

   Available Since

   March 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pAG415Gal_Gcn4 WW+

   Plasmid

   #232984

   Purpose

   Galactose iduced expression of Gcn4 WW+ in yeast

   Depositor

   Tanja Mittag, Aseem Ansari

   Article

   Bremer et al bioRxiv 2024.11.21.624739

   Insert

   Gcn4 WW+
   

   Tags

   TEV cleavage site

   Expression

   Yeast

   Mutation

   E109N, S117D, T121W, N126W, T132W

   Promoter

   Gal1

   Available Since

   March 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pAG415Gal_Gcn4 F108A+

   Plasmid

   #232974

   Purpose

   Galactose iduced expression of Gcn4 F108A in yeast

   Depositor

   Tanja Mittag, Aseem Ansari

   Article

   Bremer et al bioRxiv 2024.11.21.624739

   Insert

   Gcn4 F108A
   

   Tags

   TEV cleavage site

   Expression

   Yeast

   Mutation

   F108A, T121L, A141P

   Promoter

   Gal1

   Available Since

   March 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pAG415Gal_Gcn4 solvvol W

   Plasmid

   #232977

   Purpose

   Galactose iduced expression of Gcn4 solvvol W+ in yeast

   Depositor

   Tanja Mittag, Aseem Ansari

   Article

   Bremer et al bioRxiv 2024.11.21.624739

   Insert

   Gcn4 solvvol W+
   

   Tags

   3xHA and TEV cleavage site

   Expression

   Yeast

   Mutation

   S101T, D103E, S104T, D115E, N116Q, S117T, S122T, …

   Promoter

   Gal1

   Available Since

   March 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pAG415Gal_DBDGFP

   Plasmid

   #232999

   Purpose

   Galactose iduced expression of Gcn4 DBDGFP in yeast

   Depositor

   Tanja Mittag, Aseem Ansari

   Article

   Bremer et al bioRxiv 2024.11.21.624739

   Insert

   DBD
   

   Tags

   HA-GFP-HA and TEV cleavage site

   Expression

   Yeast

   Promoter

   Gal1

   Available Since

   March 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pAG415Gal_Gcn4 ILVtoAS

   Plasmid

   #232959

   Purpose

   Galactose iduced expression of Gcn4 ILVtoAS in yeast

   Depositor

   Tanja Mittag, Aseem Ansari

   Article

   Bremer et al bioRxiv 2024.11.21.624739

   Insert

   Gcn4 ILVtoAS
   

   Tags

   TEV cleavage site

   Expression

   Yeast

   Mutation

   L123S, I128S, V130A, V135A, L137S, I142S

   Promoter

   Gal1

   Available Since

   March 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pAG415Gal_Gcn4 WT 44merGFP

    Plasmid

    #233000

    Purpose

    Galactose iduced expression of Gcn4 WT 44merGFP in yeast

    Depositor

    Tanja Mittag, Aseem Ansari

    Article

    Bremer et al bioRxiv 2024.11.21.624739

    Insert

    Gcn4 WT 44mer
    

    Tags

    HA-GFP-HA and TEV cleavage site

    Expression

    Yeast

    Promoter

    Gal1

    Available Since

    March 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pAG415Gal_Gcn4 WT 30mer

    Plasmid

    #232986

    Purpose

    Galactose iduced expression of Gcn4 WT 30mer in yeast

    Depositor

    Tanja Mittag, Aseem Ansari

    Article

    Bremer et al bioRxiv 2024.11.21.624739

    Insert

    Gcn4 WT 30mer
    

    Tags

    TEV cleavage site

    Expression

    Yeast

    Promoter

    Gal1

    Available Since

    March 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pRS424 Prp8 WT TRP1

    Plasmid

    #234120

    Purpose

    The N-terminus of Prp8 was restored thereby destroying the NheI site and correcting the ORF

    Depositor

    Aaron Hoskins

    Insert

    Prp8
    

    Expression

    Yeast

    Available Since

    March 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pML107-ARB1

    Plasmid

    #232888

    Purpose

    Plasmid expressing Cas9 and gRNA CAAAAACTAACTGCTTACGG which targets the ARB1 gene.

    Depositor

    Jolanda Van Leeuwen

    Insert

    ARB1 gRNA (ARB1 Budding Yeast)
    

    Use

    CRISPR

    Expression

    Yeast

    Promoter

    snR52

    Available Since

    March 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pFA6a-FLAG-APEX2-NES-HIS3

    Plasmid

    #229230

    Purpose

    Template plasmid for the endogenous tagging with FLAG-APEX2-NAS in yeast

    Depositor

    Fulvio Reggiori

    Article

    Filali-Mouncef et al Autophagy. 2024 Oct;20(10):2323-2337. do

    Type

    Empty backbone

    Expression

    Yeast

    Available Since

    March 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pRS413-CUP1pr-NFAPoptim

    Plasmid

    #221110

    Purpose

    FAP insert for N-terminally tagging genes of interest (optimized FAP for yeast expression + 2xMYC tag)

    Depositor

    Allyson O’Donnell

    Article

    Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

    Type

    Empty backbone

    Expression

    Yeast

    Available Since

    March 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pRS413-CUP1pr-CFAPoptim

    Plasmid

    #221111

    Purpose

    FAP insert for C-terminally tagging genes of interest (optimized FAP for yeast expression + 2xMYC tag)

    Depositor

    Allyson O’Donnell

    Article

    Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

    Type

    Empty backbone

    Expression

    Yeast

    Available Since

    March 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pRS413-TEF1pr-ANP1-FAPoptim

    Plasmid

    #221120

    Purpose

    FAP-tagged marker for the cis-Golgi (FAP optimized for yeast expression)

    Depositor

    Allyson O’Donnell

    Article

    Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

    Insert

    ANP1-FAPoptim
    

    Expression

    Yeast

    Promoter

    TEF1

    Available Since

    March 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pRS413-TEF1pr-ERG6-FAPoptim

    Plasmid

    #221121

    Purpose

    FAP-tagged marker for lipid droplets (FAP optimized for yeast expression)

    Depositor

    Allyson O’Donnell

    Article

    Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

    Insert

    ERG6-FAPoptim
    

    Expression

    Yeast

    Promoter

    TEF1

    Available Since

    March 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pRS413-TEF1pr-PMA1-FAPoptim

    Plasmid

    #221122

    Purpose

    FAP-tagged marker for the plasma membrane (FAP optimized for yeast expression)

    Depositor

    Allyson O’Donnell

    Article

    Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

    Insert

    PMA1-FAPoptim
    

    Expression

    Yeast

    Promoter

    TEF1

    Available Since

    March 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pRS413-TEF1pr-RPA34-FAPoptim

    Plasmid

    #221123

    Purpose

    FAP-tagged marker for the nucleus (FAP optimized for yeast expression)

    Depositor

    Allyson O’Donnell

    Article

    Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

    Insert

    RPA34-FAPoptim
    

    Expression

    Yeast

    Promoter

    TEF1

    Available Since

    March 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge