8,113 results
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Plasmid#228686PurposepGAL1 Promoter for yeast expression with Nterminal tagDepositorInsertpGAL1
ExpressionYeastMutationWTAvailable SinceDec. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMY.006
Plasmid#228685PurposepTEF2 Promoter for yeast expression with Nterminal tagDepositorInsertpTEF2
ExpressionYeastMutationWTAvailable SinceDec. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMY.005
Plasmid#228684PurposepTEF1 Promoter for yeast expression with Nterminal tagDepositorInsertpTEF1
ExpressionYeastMutationWTAvailable SinceDec. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMY.004
Plasmid#228683PurposepHHF2 Promoter for yeast expression with Nterminal tagDepositorInsertpHHF2
ExpressionYeastMutationWTAvailable SinceDec. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMY.003
Plasmid#228682PurposepPGK1 Promoter for yeast expression with Nterminal tagDepositorInsertpPGK1
ExpressionYeastMutationWTAvailable SinceDec. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMY.001
Plasmid#228680PurposepTDH3 Promoter for yeast expression with Nterminal tagDepositorInsertpTDH3
ExpressionYeastMutationWTAvailable SinceDec. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCfb13239
Plasmid#219912PurposeThe base plasmid of TUNEYALI for TF49DepositorInsertContains gRNA targeting TF49 (YALI1_C04143g) and homologous arm matching TF49
ExpressionYeastAvailable SinceDec. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
Tol2-TLCV2
Plasmid#196331Purposethe TLCV2 plasmid with Tol2 recombination sites for integrationDepositorInsertCas9-2A-eGFP
UseCRISPR; Fish expressionExpressionBacterial, Mammalian, and Y…PromoterTight TRE promoterAvailable SinceDec. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPICZ A-SLC-His+Strep-Opa1 (SB254)
Plasmid#227603PurposeInducible coexpression of His-tagged SLC25A46 and Strep-tagged Opa1 in Pichia pastorisDepositorInsertsTags10xHis and Twin-StrepTagExpressionYeastMutationcontains aa 253-960 onlyPromoterAOX1Available SinceDec. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPICZ A-SLC[∆2-83]-His+MFN2-Strep (SB259)
Plasmid#227608PurposeInducible coexpression of His-tagged SLC25A46 in its N-terminal region (∆2-83) and Strep-tagged MFN2 in Pichia pastorisDepositorTags10xHis and Twin-StrepTagExpressionYeastMutationaa 2-83 deletedPromoterAOX1Available SinceDec. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPICZ A-SLC-His+MFN2[∆TM]-Strep (SB260)
Plasmid#227609PurposeInducible coexpression of His-tagged SLC25A46 and Strep-tagged MFN2 lacking its transmembrane region in Pichia pastorisDepositorTags10xHis and Twin-StrepTagExpressionYeastMutationcontains aa 2-544, 651-757 only [TM deleted]PromoterAOX1Available SinceDec. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPICZ A-SLC-His+MFN2-Strep (SB255)
Plasmid#227604PurposeInducible coexpression of His-tagged SLC25A46 and Strep-tagged MFN2 in Pichia pastorisDepositorTags10xHis and Twin-StrepTagExpressionYeastPromoterAOX1Available SinceDec. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPICZ A-SLC(2-88)-MBP-His+MFN2-Strep (SB262)
Plasmid#227611PurposeInducible coexpression of His-tagged SLC25A46 truncated to its N-terminal region (2-88) fused to MBP and MFN2-Strep in Pichia pastorisDepositorTagsMBP, 10xHis and Twin-StrepTagExpressionYeastMutationcontains aa 2-88 onlyPromoterAOX1Available SinceDec. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPICZ A-SLC-His+MFN2[∆TM,∆HB2]-Strep (SB261)
Plasmid#227610PurposeInducible coexpression of His-tagged SLC25A46 and Strep-tagged MFN2 lacking its transmembrane region and helical bundle 2 (HB2) in Pichia pastorisDepositorTags10xHis and Twin-StrepTagExpressionYeastMutationcontains aa 2-400, 706-757 [TM and HB2 deleted]PromoterAOX1Available SinceDec. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPICZ A-SLC25A46[∆2-83]-His (SB258)
Plasmid#227607PurposeInducible expression of His-tagged SLC25A46 lacking its N-terminal region (∆2-83) in Pichia pastorisDepositorInsertSLC25A46 (SLC25A46 Human)
Tags10xHisExpressionYeastMutationaa 2-83 deletedPromoterAOX1Available SinceDec. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFa6-TID-FRB-NLS-3HA-KanMX6
Plasmid#228022PurposeTo integrate TID-FRB-NLS fusion at yeast promoter; yeast BioID with KanMX6 markerDepositorInsertTurboID-FRB
TagsHA tagExpressionYeastAvailable SinceDec. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pWR58
Plasmid#202795Purposeintegrating bioluminescene reporter for Cas9 activity in S. stipitisDepositorInsertsP-TDH3/coCBG(first portion)-YUM1
P-TEF1/ShBle/T-ACT1
YUM1-coCBG(last portion)/T-ADH2
URA3 and flanking sequence
ExpressionYeastAvailable SinceDec. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAM198
Plasmid#227657PurposepRS305 His-HRV3C-Rpn5 WTDepositorAvailable SinceDec. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAM200
Plasmid#227659PurposepRS305 3XFLAG-HRV3C-Rpn5 WTDepositorAvailable SinceDec. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -