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pReporter_ZF2_mNeonGreen_ZF1

Plasmid

#213837

Purpose

Facilitates genomic integration of the reporter for screen 4: ZF-2 upstream of the reporter gene and ZF-1 downstream.

Depositor

James Collins

Article

Alcantar et al Mol Cell. 2024 Jun 20;84(12):2382-2396.e

Insert

mNeonGreen

Expression

Yeast

Available Since

June 21, 2024

Availability

Academic Institutions and Nonprofits only

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pReporter_mNeonGreen

Plasmid

#213838

Purpose

Facilitates genomic integration of the control reporter without zinc finger binding sites.

Depositor

James Collins

Article

Alcantar et al Mol Cell. 2024 Jun 20;84(12):2382-2396.e

Insert

mNeonGreen

Expression

Yeast

Available Since

June 21, 2024

Availability

Academic Institutions and Nonprofits only

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pPtet-1_mNeonGreen_Ptet-2_mCherry

Plasmid

#213839

Purpose

Expression of mNeonGreen and mCherry from aTc-inducible Ptet promoters.

Depositor

James Collins

Article

Alcantar et al Mol Cell. 2024 Jun 20;84(12):2382-2396.e

Insert

mNeonGreen, mCherry

Expression

Yeast

Available Since

June 21, 2024

Availability

Academic Institutions and Nonprofits only

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pReporter_ZF1_mNeonGreen

Plasmid

#213840

Purpose

Facilitates genomic integration of the reporter with upstream binding sites for ZF-1

Depositor

James Collins

Article

Alcantar et al Mol Cell. 2024 Jun 20;84(12):2382-2396.e

Insert

mNeonGreen

Expression

Yeast

Available Since

June 21, 2024

Availability

Academic Institutions and Nonprofits only

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pReporter_ZF2_mNeonGreen

Plasmid

#213841

Purpose

Facilitates genomic integration of the reporter with upstream binding sites for ZF-2

Depositor

James Collins

Article

Alcantar et al Mol Cell. 2024 Jun 20;84(12):2382-2396.e

Insert

mNeonGreen

Expression

Yeast

Available Since

June 21, 2024

Availability

Academic Institutions and Nonprofits only

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pPtet-2-VP16

Plasmid

#213842

Purpose

Used for expressing VP16 fused to ZF-2.

Depositor

James Collins

Article

Alcantar et al Mol Cell. 2024 Jun 20;84(12):2382-2396.e

Insert

VP16

Expression

Yeast

Available Since

June 21, 2024

Availability

Academic Institutions and Nonprofits only

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pPtet-1-VP16

Plasmid

#213843

Purpose

Used for expressing VP16 fused to ZF-1.

Depositor

James Collins

Article

Alcantar et al Mol Cell. 2024 Jun 20;84(12):2382-2396.e

Insert

VP16

Expression

Yeast

Available Since

June 21, 2024

Availability

Academic Institutions and Nonprofits only

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pStorage_Ptet-1_ZF1

Plasmid

#213844

Purpose

CombiGEM storage plasmid for ZF-1 CR fusions.

Depositor

James Collins

Article

Alcantar et al Mol Cell. 2024 Jun 20;84(12):2382-2396.e

Type

Empty backbone

Expression

Yeast

Available Since

June 21, 2024

Availability

Academic Institutions and Nonprofits only

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pStorage_Ptet-2_ZF2

Plasmid

#213845

Purpose

CombiGEM storage plasmid for ZF-2 CR fusions.

Depositor

James Collins

Article

Alcantar et al Mol Cell. 2024 Jun 20;84(12):2382-2396.e

Type

Empty backbone

Expression

Yeast

Available Since

June 21, 2024

Availability

Academic Institutions and Nonprofits only

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pReporter_TetR

Plasmid

#213847

Purpose

Facilitates genomic integration of tetR expression cassette.

Depositor

James Collins

Article

Alcantar et al Mol Cell. 2024 Jun 20;84(12):2382-2396.e

Insert

TetR

Expression

Yeast

Available Since

June 21, 2024

Availability

Academic Institutions and Nonprofits only

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pCfb12622

Plasmid

#219929

Purpose

PrACT with the overhangs for SapI restriction site can be insert into the base plasmid of TUNEYALI

Depositor

Irina Borodina

Article

Jiang et al Proc Natl Acad Sci U S A. 2025 Jun 10;12

Insert

Promoter ACT

Expression

Yeast

Available Since

June 13, 2024

Availability

Academic Institutions and Nonprofits only

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pCfb13237

Plasmid

#219910

Purpose

The base plasmid of TUNEYALI for TF47

Depositor

Irina Borodina

Article

Jiang et al Proc Natl Acad Sci U S A. 2025 Jun 10;12

Insert

Contains gRNA targeting TF47 (YALI1_C19151g) and homologous arm matching TF47

Expression

Yeast

Available Since

June 12, 2024

Availability

Academic Institutions and Nonprofits only

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pCfb12562

Plasmid

#219923

Purpose

PrGPD with the overhangs for SapI restriction site can be insert into the base plasmid of TUNEYALI

Depositor

Irina Borodina

Article

Jiang et al Proc Natl Acad Sci U S A. 2025 Jun 10;12

Insert

Promoter GPD

Expression

Yeast

Available Since

June 12, 2024

Availability

Academic Institutions and Nonprofits only

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ClhN-p500-Erg6-V5-APEX2-URA

Plasmid

#207070

Purpose

Expressing p500-Erg6-V5-APEX2. Stains cell peripheral in addition to lipid droplets.

Depositor

Zhiping Xie

Article

Li et al mSphere. 2022 Aug 31;7(4):e0010722. doi:

Insert

Erg6

Tags

V5-APEX2

Expression

Yeast

Promoter

pERG6

Available Since

June 12, 2024

Availability

Academic Institutions and Nonprofits only

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BS-ZJLEU-PTEF1-GFP

Plasmid

#207018

Purpose

Over-expression of GFP under TEF1 promoter, use auxotrophic marker LEU2(Saccharomyces cerevisiae).

Depositor

Zhiping Xie

Article

He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

Insert

GFP

Expression

Yeast

Promoter

pTEF1

Available Since

June 12, 2024

Availability

Academic Institutions and Nonprofits only

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PTPI1-Dsred-URA3

Plasmid

#207017

Purpose

Expresses DsRed-Express 2 as a marker of cytosol, uses auxotrophic marker URA3(Saccharomyces cerevisiae).

Depositor

Zhiping Xie

Article

He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

Insert

DsRed-Express 2

Expression

Yeast

Promoter

pTPI1

Available Since

June 12, 2024

Availability

Academic Institutions and Nonprofits only

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pCfb13186

Plasmid

#219924

Purpose

PrTEF with the overhangs for SapI restriction site can be insert into the base plasmid of TUNEYALI

Depositor

Irina Borodina

Article

Jiang et al Proc Natl Acad Sci U S A. 2025 Jun 10;12

Insert

Promoter TEF

Expression

Yeast

Available Since

June 12, 2024

Availability

Academic Institutions and Nonprofits only

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pCfb13204

Plasmid

#219879

Purpose

The base plasmid of TUNEYALI for TF14

Depositor

Irina Borodina

Article

Jiang et al Proc Natl Acad Sci U S A. 2025 Jun 10;12

Insert

Contains gRNA targeting TF14 (YALI1_F21261g) and homologous arm matching TF14

Expression

Yeast

Available Since

June 12, 2024

Availability

Academic Institutions and Nonprofits only

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pCfb13200

Plasmid

#219875

Purpose

The base plasmid of TUNEYALI forTF10

Depositor

Irina Borodina

Article

Jiang et al Proc Natl Acad Sci U S A. 2025 Jun 10;12

Insert

Contains gRNA targeting TF10 ( YALI1_C16204g) and homologous arm matching TF10

Expression

Yeast

Available Since

June 11, 2024

Availability

Academic Institutions and Nonprofits only

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pCfb13242

Plasmid

#219914

Purpose

The base plasmid of TUNEYALI for TF52

Depositor

Irina Borodina

Article

Jiang et al Proc Natl Acad Sci U S A. 2025 Jun 10;12

Insert

Contains gRNA targeting TF52 (YALI1_E12668g) and homologous arm matching TF52

Expression

Yeast

Available Since

June 11, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge