8,430 results
-
Plasmid#219896PurposeThe base plasmid of TUNEYALI for TF31DepositorInsertContains gRNA targeting TF31 (YALI1_D18727g) and homologous arm matching TF31
ExpressionYeastAvailable sinceJune 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCfb13235
Plasmid#219908PurposeThe base plasmid of TUNEYALI for TF45DepositorInsertContains gRNA targeting TF45 ( YALI1_B23373g) and homologous arm matching TF45
ExpressionYeastAvailable sinceJune 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCfb13212
Plasmid#219887PurposeThe base plasmid of TUNEYALI for TF22DepositorInsertContains gRNA targeting TF22 (YALI1_C25288g) and homologous arm matching TF22
ExpressionYeastAvailable sinceJune 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCfb13202
Plasmid#219877PurposeThe base plasmid of TUNEYALI forTF12DepositorInsertContains gRNA targeting TF12 (YALI1_C06870g) and homologous arm matching TF12
ExpressionYeastAvailable sinceJune 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCfb13234
Plasmid#219907PurposeThe base plasmid of TUNEYALI for TF44DepositorInsertContains gRNA targeting TF44 (YALI1_B22561g) and homologous arm matching TF44
ExpressionYeastAvailable sinceJune 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCfb13219
Plasmid#219894PurposeThe base plasmid of TUNEYALI for TF29DepositorInsertContains gRNA targeting TF29 (YALI1_C09133g) and homologous arm matching TF29
ExpressionYeastAvailable sinceJune 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCfb13248
Plasmid#219919PurposeThe base plasmid of TUNEYALI for TF58DepositorInsertContains gRNA targeting TF58 (YALI1_F37742g) and homologous arm matching TF58
ExpressionYeastAvailable sinceJune 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
BS-TRP1-PGAL-GFP-Cps1
Plasmid#207030PurposeExpression of GFP-Cps1 under GAL promoter.DepositorInsertCps1
TagsGFPExpressionYeastPromoterGAL1Available sinceJune 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
Ypq2-2GFP-URA
Plasmid#207010PurposeIntegration of 2xGFP tag at YPQ2 C terminus, use auxotrophic marker URA3(Kluyveromyces lactis) for selection. Marker for yeast vacuolar membrane.DepositorInsertYPQ2
Tags2xGFPExpressionYeastAvailable sinceJune 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSCL475
Plasmid#219503PurposeExpress Ade2, Can1 and Faa1 retron msd donor-gRNAs from a single Gal7 promoter, separated by a Csy4 recognition sequence, with an msr expressed in transDepositorInsertAde2, Can1 and Faa1 retron msd donor-gRNAs separated by a Csy4 recognition sequence, with an msr expressed in trans
ExpressionYeastPromoterGAL7Available sinceMay 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSCL452
Plasmid#219502PurposeGolden Gate entry vector for library cloning of msd-sgRNA arrays: GAL7p-Csy4-PaqCI-PaqCI-GAL7t // SNR52p-msr-SUP4tDepositorInsertGAL7p-Csy4-PaqCI-PaqCI-GAL7t // SNR52p-msr-SUP4t
ExpressionYeastPromoterGAL7Available sinceMay 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSCL390
Plasmid#219500PurposeIntegrating inducible cassette (Cas9-P2A-Csy4 and Eco1 RT) for yeast overexpressionDepositorInsertIntegrating inducible cassette: Cas9-P2A-Csy4 and Eco1 RT
TagsSV40-NLSExpressionYeastPromoterGAL1-GAL10Available sinceMay 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSCL391
Plasmid#219501PurposeExpress Ade2 and Faa1 editrons from a single Gal7 promoter, separated by a Csy4 recognition sequenceDepositorInsertEco1 editing ncRNAs and Cas9 gRNAs to edit ADE2 and FAA1
ExpressionYeastPromoterGAL7Available sinceMay 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKL1-100
Plasmid#219408PurposeReporter gene (4 operators) for zinc finger activatorDepositorInsert(42-10 op)x4-minCyc1-Venus-tCyc1
ExpressionYeastAvailable sinceMay 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKL1-422
Plasmid#219410PurposeReporter gene (1 operator) for zinc finger activatorDepositorInsert(42-10 op)x1-minCyc1-Venus-tCyc1
ExpressionYeastAvailable sinceMay 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKL1-424
Plasmid#219412PurposeReporter gene (3 operator) for zinc finger activatorDepositorInsert(42-10 op)x3-minCyc1-Venus-tCyc1
ExpressionYeastAvailable sinceMay 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKL1-095
Plasmid#219407PurposeReporter gene (4 operators) for zinc finger activatorDepositorInsert(13-6 op)x4-minCyc1-Venus-tCyc1
ExpressionYeastAvailable sinceMay 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKL1-423
Plasmid#219411PurposeReporter gene (2 operator) for zinc finger activatorDepositorInsert(42-10 op)x2-minCyc1-Venus-tCyc1
ExpressionYeastAvailable sinceMay 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKL2-138
Plasmid#219413PurposeEstradiol-inducible expression of 42-10 Zinc finger activatorDepositorInsert{pTEF1-ZEV}{pGal1(Zif268op)x6-3xFLAG-NLS-VP16-mRuby2-(42-10-4x)-PDZLigWT-tSSA1}
ExpressionYeastMutationFour Arg to Ala mutations in zinc finger backboneAvailable sinceMay 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKL2-160
Plasmid#219414PurposeEstradiol-inducible expression of 13-6 Zinc finger activatorDepositorInsert{pTEF1-ZEV}{pGal1(Zif268op)x6-3xFLAG-NLS-VP16-mRuby2-(42-10-WT)-PDZLigWT-tSSA1}
ExpressionYeastAvailable sinceMay 24, 2024AvailabilityAcademic Institutions and Nonprofits only