8,060 results

1. pACT2-μ4

   Plasmid

   #198176

   Purpose

   Expression of GAL4 transcriptional activation domain (AD)-AP-4 μ4 fusion protein in yeast (yeast two-hybrid assays)

   Depositor

   Juan Bonifacino

   Article

   Guo et al Dev Cell. 2013 Nov 11;27(3):353-66. doi:

   Insert

   AP-4 μ4
   

   Use

   Tags

   GAL4 transcriptional activation domain (AD) fragm…

   Expression

   Yeast

   Mutation

   Promoter

   ADH1

   Available since

   April 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pCEV-G2-Km ymTurquoise2

   Plasmid

   #193958

   Purpose

   TEF1 promoter controlled expression plasmid with G418 resistance for expressing ymTurquoise2 in budding yeast

   Depositor

   Bas Teusink

   Insert

   ymTurquoise2
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   TEF1

   Available since

   April 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pGBT9-GGA2-GAE

   Plasmid

   #199175

   Purpose

   Expression of GAL4 DNA-binding domain (BD)-GGA2-GAE domain fusion protein in yeast (yeast two-hybrid assays)

   Depositor

   Juan Bonifacino

   Article

   Mattera et al EMBO J. 2003 Jan 2;22(1):78-88. doi: 10.

   Insert

   GGA2-GAE domain (GGA2 Human)
   

   Use

   Tags

   GAL4-DNA binding domain fragment

   Expression

   Yeast

   Mutation

   Contains Lys 545 Gln substitution

   Promoter

   ADH1

   Available since

   April 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pGBT9-γ1 ear

   Plasmid

   #199177

   Purpose

   Expression of GAL4 DNA-binding domain (BD)-AP-1 γ1 ear domain fusion protein in yeast (yeast two-hybrid assays)

   Depositor

   Juan Bonifacino

   Article

   Mattera et al EMBO J. 2003 Jan 2;22(1):78-88. doi: 10.

   Insert

   AP-1 γ1 ear domain (Ap1g1 Mouse)
   

   Use

   Tags

   GAL4-DNA binding domain fragment

   Expression

   Yeast

   Mutation

   Promoter

   ADH1

   Available since

   April 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pGBT9-GGA3-GAE

   Plasmid

   #199176

   Purpose

   Expression of GAL4 DNA-binding domain (BD)-GGA3-GAE domain fusion protein in yeast (yeast two-hybrid assays)

   Depositor

   Juan Bonifacino

   Article

   Mattera et al EMBO J. 2003 Jan 2;22(1):78-88. doi: 10.

   Insert

   GGA3-GAE domain (GGA3 Human)
   

   Use

   Tags

   GAL4-DNA binding domain fragment

   Expression

   Yeast

   Mutation

   Promoter

   ADH1

   Available since

   April 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pGBT9-γ2 ear

   Plasmid

   #199178

   Purpose

   Expression of GAL4 DNA-binding domain (BD)-AP-1 γ2 ear domain fusion protein in yeast (yeast two-hybrid assays)

   Depositor

   Juan Bonifacino

   Article

   Mattera et al EMBO J. 2003 Jan 2;22(1):78-88. doi: 10.

   Insert

   AP-1 γ2 ear domain (AP1G2 Human)
   

   Use

   Tags

   GAL4-DNA binding domain fragment

   Expression

   Yeast

   Mutation

   Promoter

   ADH1

   Available since

   April 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pGBT9-GGA1-GAE

   Plasmid

   #199174

   Purpose

   Expression of GAL4 DNA-binding domain (BD)-GGA1-GAE domain fusion protein in yeast (yeast two-hybrid assays)

   Depositor

   Juan Bonifacino

   Article

   Mattera et al EMBO J. 2003 Jan 2;22(1):78-88. doi: 10.

   Insert

   GGA1-GAE domain (GGA1 Human)
   

   Use

   Tags

   GAL4-DNA binding domain fragment

   Expression

   Yeast

   Mutation

   Promoter

   ADH1

   Available since

   April 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pGBT9-ATG9A-N

   Plasmid

   #198530

   Purpose

   Expression of GAL4 DNA-binding domain (BD)-ATG9A-N terminal cytosolic domain fusion protein in yeast (yeast two-hybrid assays)

   Depositor

   Juan Bonifacino

   Article

   Mattera et al Proc Natl Acad Sci U S A. 2017 Dec 12;11

   Insert

   ATG9A N-terminal cytosolic tail (residues 1-66) (ATG9A Human)
   

   Use

   Tags

   GAL4-DNA binding domain fragment

   Expression

   Yeast

   Mutation

   Promoter

   ADH1

   Available since

   April 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pGBT9-FTS

   Plasmid

   #198526

   Purpose

   Expression of GAL4 DNA-binding domain (BD)-FTS fusion protein in yeast (yeast two-hybrid assays)

   Depositor

   Juan Bonifacino

   Article

   Mattera et al Mol Biol Cell. 2020 Apr 15;31(9):963-979

   Insert

   FTS (AKTIP Human)
   

   Use

   Tags

   GAL4-DNA binding domain fragment

   Expression

   Yeast

   Mutation

   Promoter

   ADH1

   Available since

   April 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pGBT9-FHIP-L

    Plasmid

    #198528

    Purpose

    Expression of GAL4 DNA-binding domain (BD)-FHIP-L fusion protein in yeast (yeast two-hybrid assays)

    Depositor

    Juan Bonifacino

    Article

    Mattera et al Mol Biol Cell. 2020 Apr 15;31(9):963-979

    Insert

    FHIP-L (FHIP1A Human)
    

    Use

    Tags

    GAL4-DNA binding domain fragment

    Expression

    Yeast

    Mutation

    Promoter

    ADH1

    Available since

    April 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pGBT9-Hook1

    Plasmid

    #198523

    Purpose

    Expression of GAL4 DNA-binding domain (BD)-Hook1 fusion protein in yeast (yeast two-hybrid assays)

    Depositor

    Juan Bonifacino

    Article

    Mattera et al Mol Biol Cell. 2020 Apr 15;31(9):963-979

    Insert

    Hook1 (HOOK1 Human)
    

    Use

    Tags

    GAL4-DNA binding domain fragment

    Expression

    Yeast

    Mutation

    silent substitution in codon 620 to eliminate int…

    Promoter

    ADH1

    Available since

    April 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pGADT7-δ

    Plasmid

    #197259

    Purpose

    Expression of GAL4 transcriptional activation domain (AD)-AP-3 δ fusion protein in yeast (yeast two-hybrid or yeast three-hybrid assays)

    Depositor

    Juan Bonifacino

    Article

    Mattera et al J Biol Chem. 2011 Jan 21;286(3):2022-30.

    Insert

    AP-3 δ (AP3D1 Human)
    

    Use

    Tags

    GAL4 transcriptional activation domain (AD) fragm…

    Expression

    Yeast

    Mutation

    Val 549 Ile substitution; silent substitutions in…

    Promoter

    ADH1

    Available since

    March 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pGAD424-Rabex-5

    Plasmid

    #197043

    Purpose

    Expression of GAL4 transcriptional activation domain (AD)-Rabex-5 fusion protein in yeast (yeast two-hybrid assays)

    Depositor

    Juan Bonifacino

    Article

    Mattera et al J Biol Chem. 2006 Mar 10;281(10):6874-83

    Insert

    Rabex-5 (RABGEF1 Bovine)
    

    Use

    Tags

    GAL4 transcriptional activation domain (AD) fragm…

    Expression

    Yeast

    Mutation

    Promoter

    ADH1

    Available since

    March 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pBridge-tyrosinase.σ3A

    Plasmid

    #197513

    Purpose

    Expression of 1) GAL4 DNA-binding domain (BD)-tyrosinase cytosolic tail fusion protein, 2) HA epitope and nuclear localization signal (NLS) AP-3 σ3A fusion protein in yeast (yeast three-hybrid assays)

    Depositor

    Juan Bonifacino

    Article

    Mattera et al J Biol Chem. 2011 Jan 21;286(3):2022-30.

    Inserts

    tyrosinase cytosolic tail
    AP-3 σ3A
    

    Use

    Tags

    GAL4-DNA binding domain fragment, HA tag, and nuc…

    Expression

    Yeast

    Mutation

    silent substitution in codon 2 of tyrosinase tail…

    Promoter

    ADH1 and MET25

    Available since

    March 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pROS_phleo-Tps1/Gsy2

    Plasmid

    #196612

    Purpose

    Encoding guide RNAs for the knock out of TPS1 and GSY2 genes

    Depositor

    Matthias Heinemann

    Article

    Takhaveev et al Nat Metab. 2023 Feb;5(2):294-313. doi: 1

    Insert

    TPS1, GSY2 (TPS1, GSY2 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    SNR52

    Available since

    March 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pGADT7-AP-4 β4

    Plasmid

    #198327

    Purpose

    Expression of GAL4 transcriptional activation domain (AD)-AP-4 β4 fusion protein in yeast (yeast two-hybrid assays)

    Depositor

    Juan Bonifacino

    Article

    Mattera et al Mol Biol Cell. 2022 Oct 1;33(12):ar109.

    Insert

    AP-4 β4
    

    Use

    Tags

    GAL4 transcriptional activation domain (AD) fragm…

    Expression

    Yeast

    Mutation

    Contains L480S substitution

    Promoter

    ADH1

    Available since

    March 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pGAD424-AP-4 σ4

    Plasmid

    #198328

    Purpose

    Expression of GAL4 transcriptional activation domain (AD)-AP-4 σ4 fusion protein in yeast (yeast two-hybrid assays)

    Depositor

    Juan Bonifacino

    Article

    Mattera et al Mol Biol Cell. 2022 Oct 1;33(12):ar109.

    Insert

    AP-4 σ4
    

    Use

    Tags

    GAL4 transcriptional activation domain (AD) fragm…

    Expression

    Yeast

    Mutation

    Promoter

    ADH1

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pGADT7-AP-2 σ2

    Plasmid

    #198345

    Purpose

    Expression of GAL4 transcriptional activation domain (AD)-AP-2 σ2 fusion protein in yeast (yeast two-hybrid assays)

    Depositor

    Juan Bonifacino

    Article

    Mattera et al Mol Biol Cell. 2022 Oct 1;33(12):ar109.

    Insert

    AP-2 σ2
    

    Use

    Tags

    GAL4 transcriptional activation domain (AD) fragm…

    Expression

    Yeast

    Mutation

    R42G substitution, and silent substitutions in co…

    Promoter

    ADH1

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pGADT7-AP-1 σ1B

    Plasmid

    #198335

    Purpose

    Expression of GAL4 transcriptional activation domain (AD)-AP-1 σ1B fusion protein in yeast (yeast two-hybrid assays)

    Depositor

    Juan Bonifacino

    Article

    Mattera et al Mol Biol Cell. 2022 Oct 1;33(12):ar109.

    Insert

    AP-1 σ1B
    

    Use

    Tags

    GAL4 transcriptional activation domain (AD) fragm…

    Expression

    Yeast

    Mutation

    Expresses a LELQMNRRY nonapeptide after the last …

    Promoter

    ADH1

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pGADT7-AP-1 σ1A

    Plasmid

    #198334

    Purpose

    Expression of GAL4 transcriptional activation domain (AD)-AP-1 σ1A fusion protein in yeast (yeast two-hybrid assays)

    Depositor

    Juan Bonifacino

    Article

    Mattera et al Mol Biol Cell. 2022 Oct 1;33(12):ar109.

    Insert

    AP-1 σ1A
    

    Use

    Tags

    GAL4 transcriptional activation domain (AD) fragm…

    Expression

    Yeast

    Mutation

    Expresses a LELQMNRRY nonapeptide after the last …

    Promoter

    ADH1

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge