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pYea5.1_Notch1_sfGFP

Plasmid

#207801

Purpose

Yeast 2µ vector for expression of Suc2SP-Notch1_100-sfGFP-GAL4

Depositor

Dieter Langosch

Article

Ortner et al J Mol Biol. 2023 Aug 1;435(18):168218. d

Insert

Suc2SP-Notch1_100-sfGFP-GAL4 fusion protein

Tags

Strep-TagII

Expression

Bacterial and Yeast

Promoter

TEF

Available Since

Nov. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pHYX143

Plasmid

#202816

Purpose

Control plasmid expressing mScarlet-I fluorescent protein.

Depositor

Jean-Félix Dallery

Article

Geistodt-Kiener et al Metab Eng. 2023 Oct 18:S1096-7176(23)001

Insert

mScarlet-I

Expression

Yeast

Promoter

ScPCK1

Available Since

Nov. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pHYX173

Plasmid

#202817

Purpose

Plasmid expressing the TEV protease, codon-optimized for Saccharomyces cerevisiae.

Depositor

Jean-Félix Dallery

Article

Geistodt-Kiener et al Metab Eng. 2023 Oct 18:S1096-7176(23)001

Insert

TEV protease

Expression

Yeast

Promoter

ScPCK1

Available Since

Nov. 1, 2023

Availability

Academic Institutions and Nonprofits only

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YP46

Plasmid

#199987

Purpose

Yeast/E coli shuttle vector expressing GFP from synthetic yeast promoter 46

Depositor

Farren Isaacs

Article

Patel et al Cell. 2022 Apr 28;185(9):1487-1505.e14.

Insert

GFP

Expression

Yeast

Available Since

Oct. 24, 2023

Availability

Academic Institutions and Nonprofits only

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185_pETcon_SARS2_BQ1-1

Plasmid

#208085

Purpose

yeast surface display of the SARS-CoV-2 BQ.1.1 variant RBD

Depositor

Tyler Starr

Article

Taylor et al PLoS Pathog. 2023 Dec 29;19(12):e1011901

Insert

SARS-CoV-2 BQ.1.1 RBD (S Budding Yeast, SARS-CoV-2 virus)

Tags

HA and c-Myc

Expression

Yeast

Available Since

Oct. 4, 2023

Availability

Academic Institutions and Nonprofits only

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186_pETcon_SARS2_XBB1-5

Plasmid

#208086

Purpose

yeast surface display of the SARS-CoV-2 XBB.1.5 variant RBD

Depositor

Tyler Starr

Article

Taylor et al PLoS Pathog. 2023 Dec 29;19(12):e1011901

Insert

SARS-CoV-2 XBB.1.5 RBD (S Budding Yeast, SARS-CoV-2 virus)

Tags

HA and c-Myc

Expression

Yeast

Available Since

Oct. 4, 2023

Availability

Academic Institutions and Nonprofits only

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pWR24

Plasmid

#202771

Purpose

episomal bioluminescent reporter plasmid for S. stipitis

Depositor

J. Brian Robertson

Article

Reichard et al mSphere. 2023 Aug 24;8(4):e0022423. doi:

Inserts

TEF1 promoter (S. stipitis) (TEF1 S. stipitis (yeast))
coNAT
ACT1 terminator (S. stipitis)
TDH3 promoter (S. stipitis)
coCBG
ADH2 terminator (S. stipitis)
ARS from S. stipitis chromosome 1

Expression

Yeast

Available Since

Oct. 4, 2023

Availability

Academic Institutions and Nonprofits only

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pWR95

Plasmid

#203916

Purpose

BLINCAR plasmid for gene deletion, no genomic target

Depositor

J. Brian Robertson

Article

Reichard et al mSphere. 2023 Aug 24;8(4):e0022423. doi:

Insert

modified MCS

Expression

Yeast

Available Since

Oct. 4, 2023

Availability

Academic Institutions and Nonprofits only

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pWR16

Plasmid

#202711

Purpose

integrating bioluminescent reporter plasmid for S. stipitis

Depositor

J. Brian Robertson

Article

Reichard et al mSphere. 2023 Aug 24;8(4):e0022423. doi:

Inserts

TEF1 promoter (S. stipitis) (TEF1 S. stipitis (yeast))
coHPH
ACT1 terminator (S. stipitis)
TDH3 promoter (S. stipitis)
coCBG
ADH2 terminator (S. stipitis)
URA3 and flanking sequence

Expression

Yeast

Available Since

Oct. 4, 2023

Availability

Academic Institutions and Nonprofits only

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pYP-ptef1-GFP

Plasmid

#199990

Purpose

Yeast/E coli shuttle vector expressing GFP from tef1 promoter

Depositor

Farren Isaacs

Article

Patel et al Cell. 2022 Apr 28;185(9):1487-1505.e14.

Insert

GFP

Expression

Yeast

Available Since

Sept. 29, 2023

Availability

Academic Institutions and Nonprofits only

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pUDE1093

Plasmid

#204227

Purpose

Episomal yeast plasmid expressing the Ercas12a

Depositor

Jean-Marc Daran

Article

Bennis et al FEMS Yeast Res. 2023 Jan 4;23:foad043. d

Insert

Eubacterium rectale cas12a (Ercas12a)

Expression

Yeast

Mutation

codon optimized for S. cerevisiae

Promoter

Saccharomyces cerevisiae PGK1

Available Since

Sept. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pDRF1-GW ymTq2-T2A-ymScarletl

Plasmid

#205511

Purpose

Expression of ymTq2-T2A-ymScarletl in yeast

Depositor

Bas Teusink

Insert

ymTq2-T2A-ymScarletl

Expression

Yeast

Available Since

Sept. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pDRF1-GW ymTq2-d11

Plasmid

#205490

Purpose

express ymTq2-d11 as donor control for yAT1.03

Depositor

Bas Teusink

Article

Luzia et al iScience. 2023 Dec 21;27(1):108767. doi:

Insert

ymTq2-d11

Expression

Yeast

Available Since

Sept. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pDRF1-GW ymTq2-ymScarletI

Plasmid

#205512

Purpose

Expression of ymTq2-ymScarletl in yeast

Depositor

Bas Teusink

Article

Botman et al ACS Synth Biol. 2026 Apr 1. doi: 10.1021

Insert

ymTq2-ymScarletl

Expression

Yeast

Available Since

Sept. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pDRF1-GW miFP663-T2A-mtq2

Plasmid

#205504

Purpose

Expression of miFP663-T2A-mtq2 in yeast

Depositor

Bas Teusink

Article

Botman et al ACS Synth Biol. 2026 Apr 1. doi: 10.1021

Insert

miFP663-T2A-mtq2

Expression

Yeast

Available Since

Sept. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pDRF1-GW emiRFP2-T2A-mtq2

Plasmid

#205497

Purpose

Expression of emiRFP2-T2A-mtq2 in yeast

Depositor

Bas Teusink

Article

Botman et al ACS Synth Biol. 2026 Apr 1. doi: 10.1021

Insert

emiRFP2-T2A-mtq2

Expression

Yeast

Available Since

Sept. 13, 2023

Availability

Academic Institutions and Nonprofits only

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pDRF1-GW ymTq2-P2A-ymScarletI

Plasmid

#205500

Purpose

Expression of ymTq2-P2A-ymScarletI in yeast

Depositor

Bas Teusink

Article

Botman et al ACS Synth Biol. 2026 Apr 1. doi: 10.1021

Insert

ymTq2-P2A-ymScarletI

Expression

Yeast

Available Since

Sept. 13, 2023

Availability

Academic Institutions and Nonprofits only

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pDRF1-GW ymTq2-E2A-ymScarletl

Plasmid

#205510

Purpose

Expression of ymTq2-E2A-ymScarletl in yeast

Depositor

Bas Teusink

Article

Botman et al ACS Synth Biol. 2026 Apr 1. doi: 10.1021

Insert

ymTq2-E2A-ymScarletl

Expression

Yeast

Available Since

Sept. 13, 2023

Availability

Academic Institutions and Nonprofits only

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pDRF1-GW ymTq2-O2A-ymScarletl

Plasmid

#205509

Purpose

Expression of ymTq2-O2A-ymScarletl in yeast

Depositor

Bas Teusink

Article

Botman et al ACS Synth Biol. 2026 Apr 1. doi: 10.1021

Insert

ymTq2-O2A-ymScarletl

Expression

Yeast

Available Since

Sept. 13, 2023

Availability

Academic Institutions and Nonprofits only

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pDRF1-GW Achilles-T2A-mCherry

Plasmid

#205499

Purpose

Expression of Achilles-T2A-mCherry in yeast

Depositor

Bas Teusink

Article

Botman et al ACS Synth Biol. 2026 Apr 1. doi: 10.1021

Insert

Achilles-T2A-mCherry

Expression

Yeast

Available Since

Sept. 13, 2023

Availability

Academic Institutions and Nonprofits only

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