8,060 results

1. YIplac211-AUR1-msGFP2

   Plasmid

   #177101

   Purpose

   Expresses Aur1-msGFP2 in yeast cells

   Depositor

   Benjamin Glick

   Article

   Casler et al J Cell Biol. 2022 Jan 3;221(1). pii: 212

   Insert

   AUR1 (AUR1 Budding Yeast)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Nov. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. YIplac211-RBD2-6xmGFP

   Plasmid

   #177109

   Purpose

   Expresses Rbd2-6xmEGFP in yeast cells

   Depositor

   Benjamin Glick

   Article

   Casler et al J Cell Biol. 2022 Jan 3;221(1). pii: 212

   Insert

   RBD2 (RBD2 Budding Yeast)
   

   Use

   Tags

   6xmEGFP

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Nov. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. YIplac211-SEC2-msGFPx3

   Plasmid

   #177110

   Purpose

   Expresses Sec2-msGFPx3 in yeast cells

   Depositor

   Benjamin Glick

   Article

   Casler et al J Cell Biol. 2022 Jan 3;221(1). pii: 212

   Insert

   SEC2 (SEC2 Budding Yeast)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Nov. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pDB4961

   Plasmid

   #174015

   Purpose

   pHIS3H plasmid expressing 13Myc-Vps15 from 41nmt1 promoter

   Depositor

   Li-Lin Du

   Article

   Yu et al J Cell Sci. 2021 Oct 1;134(19):jcs258774

   Insert

   Vps15 (ppk19 Fission Yeast)
   

   Use

   Tags

   13Myc, Vps15

   Expression

   Yeast

   Mutation

   Promoter

   41nmt1

   Available since

   Nov. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. EC2_2_dCas9_VP64_sgRNA

   Plasmid

   #163707

   Purpose

   Yeast low copy plasmid with dCas9, sgRNA expression cassette and VP64 activation domain

   Depositor

   Yvonne Nygård

   Article

   Camara et al Sci Rep. 2020 Sep 3;10(1):14605. doi: 10

   Inserts

   dCas9
   VP64+SV40 NLS
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Point mutations D10A and H840A

   Promoter

   ScTEF1

   Available since

   Nov. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. Ecm2 1-197

   Plasmid

   #169924

   Purpose

   WT Ecm2 mutated to introduce stop codon after AA 197 of Ecm2

   Depositor

   Aaron Hoskins

   Article

   van der Feltz et al RNA. 2021 Feb 5. pii: rna.077727.120. do

   Insert

   Ecm2 1-197
   

   Use

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   Available since

   Oct. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pRS426-CeLACE-1

   Plasmid

   #174523

   Purpose

   Express C.elegans LACE-1 in yeast cells

   Depositor

   Oleh Khalimonchuk

   Article

   Germany et al J Cell Sci. 2018 Nov 21;131(22). pii: jc

   Insert

   lace-1 (C30F12.2 Nematode)
   

   Use

   Tags

   6xHis

   Expression

   Yeast

   Mutation

   Promoter

   MET25

   Available since

   Sept. 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEDJ-87

   Plasmid

   #163085

   Purpose

   MarkerFree plasmid for integration of pADH1-MCP-VPR into site XI-5

   Depositor

   Michael Jensen

   Article

   Jensen et al Microb Cell Fact. 2017 Mar 15;16(1):46.

   Insert

   VPR
   

   Use

   Tags

   MCP

   Expression

   Yeast

   Mutation

   Promoter

   ADH1

   Available since

   Sept. 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pJo55

   Plasmid

   #159623

   Purpose

   An empty HIS3 plasmid for translation factor eIF4E complementation test in yeast, strain Jo55. Golden Gate cloning using BsaI. Selection with RFP after cloning.

   Depositor

   Vasiliy Taranov

   Article

   Lebedeva et al Biochemistry (Mosc). 2021 Sep;86(9):1128

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   Available since

   Sept. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pJo55-CA1

    Plasmid

    #159624

    Purpose

    Plasmid pJo55, which contains positive control (C. annuum eIF4E-1) for complementation test in Jo55 yeasts.

    Depositor

    Vasiliy Taranov

    Article

    Lebedeva et al Biochemistry (Mosc). 2021 Sep;86(9):1128

    Insert

    C. annuum eIF4E-1
    

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    Sept. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pIFU002

    Plasmid

    #174299

    Purpose

    Encodes the SARS-CoV-2 Spike Receptor Binding Domain (RBD) with the N501Y mutation and can be combined with pJS697 (Addgene plasmid # 168778) through homologous recombination

    Depositor

    Timothy Whitehead

    Article

    Francino-Urdaniz et al Cell Rep. 2021 Aug 31;36(9):109627. doi:

    Insert

    Spike RBD N343Q, N501Y (S Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    HA and cMyc

    Expression

    Yeast

    Mutation

    N343Q, N501Y

    Promoter

    Available since

    Sept. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pIFU001

    Plasmid

    #174298

    Purpose

    Encodes the SARS-CoV-2 Spike Receptor Binding Domain (RBD) with the E484K mutation and can be combined with pJS697 (Addgene plasmid # 168778) through homologous recombination

    Depositor

    Timothy Whitehead

    Article

    Francino-Urdaniz et al Cell Rep. 2021 Aug 31;36(9):109627. doi:

    Insert

    Spike RBD N343Q, E484K (S Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    HA and cMyc

    Expression

    Yeast

    Mutation

    N343Q, E484K

    Promoter

    Available since

    Sept. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pJL1

    Plasmid

    #174070

    Purpose

    Expresses VP16-CIB1 in S. cerevisiae. Part of optogenetic system.

    Depositor

    Albert Keung

    Article

    Lee et al Cell Syst. 2021 Aug 24. pii: S2405-4712(

    Insert

    pGal-Olac-3xNLS-VP16-CIB1-tADH1
    

    Use

    Tags

    FLAG

    Expression

    Yeast

    Mutation

    Promoter

    pGal1

    Available since

    Sept. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pJL29

    Plasmid

    #174063

    Purpose

    Expresses for mCherry for S. cerevisiae. Contain 2 43-8 zinc-finger operators upstream of Cyc1 promoter.

    Depositor

    Albert Keung

    Article

    Lee et al Cell Syst. 2021 Aug 24. pii: S2405-4712(

    Insert

    2x43-8 ZFO-1x97-4 ZFO pCyc mCherry tCyc
    

    Use

    Tags

    FLAG

    Expression

    Yeast

    Mutation

    Promoter

    pCyc1

    Available since

    Sept. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pJL15

    Plasmid

    #174069

    Purpose

    Expresses 43-8 zinc finger array fused to CRY2 in S. cerevisiae. Part of optogenetic system.

    Depositor

    Albert Keung

    Article

    Lee et al Cell Syst. 2021 Aug 24. pii: S2405-4712(

    Insert

    pGAL-tetO-3xNLS-43-8WT-GS-Cry2-Tadh1
    

    Use

    Tags

    FLAG

    Expression

    Yeast

    Mutation

    Promoter

    pGal1

    Available since

    Sept. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pRS41K-mScarlet-I-mNeonGreen-ADH1term-TEFpr-hphΔC (pKBJ011)

    Plasmid

    #173462

    Purpose

    C-SWAT donor for Selection Reconstitution tagging, contains mScarletI-mNeonGreen tandem fluorescent protein timer

    Depositor

    Anton Khmelinskii

    Article

    Fung et al Methods Mol Biol. 2022;2378:85-100. doi:

    Insert

    mScarlet-I-mNeonGreen
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    no promoter

    Available since

    Sept. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pRS41K-mCherry-mNeonGreen-ADH1term-TEFpr-hphΔC (pJJF004)

    Plasmid

    #173460

    Purpose

    C-SWAT donor for Selection Reconstitution tagging, contains mCherry-mNeonGreen tandem fluorescent protein timer

    Depositor

    Anton Khmelinskii

    Article

    Fung et al Methods Mol Biol. 2022;2378:85-100. doi:

    Insert

    mCherry-mNeonGreen
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    no promoter

    Available since

    Sept. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pRS41K-mCherry-mNeonGreen (pJJF003)

    Plasmid

    #173459

    Purpose

    C-SWAT donor for Seamless tagging, contains mCherry-mNeonGreen tandem fluorescent protein timer

    Depositor

    Anton Khmelinskii

    Article

    Fung et al Methods Mol Biol. 2022;2378:85-100. doi:

    Insert

    mCherry-mNeonGreen
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    no promoter

    Available since

    Sept. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pRS41K-mCherry-sfGFP-ADH1term-TEFpr-hphΔC (pJJF002)

    Plasmid

    #173458

    Purpose

    C-SWAT donor for Selection Reconstitution tagging, contains mCherry-sfGFP tandem fluorescent protein timer

    Depositor

    Anton Khmelinskii

    Article

    Fung et al Methods Mol Biol. 2022;2378:85-100. doi:

    Insert

    mCherry-sfGFP
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    no promoter

    Available since

    Sept. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pDB4953

    Plasmid

    #174020

    Purpose

    pDUAL plasmid expressing GFP-Atg6 from 41nmt1 promoter

    Depositor

    Li-Lin Du

    Article

    Yu et al J Cell Sci. 2021 Oct 1;134(19):jcs258774

    Insert

    Atg6
    

    Use

    Tags

    GFP, Atg6

    Expression

    Yeast

    Mutation

    Promoter

    41nmt1

    Available since

    Sept. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge