8,060 results

1. Dcp2 FL-H1

   Plasmid

   #163583

   Purpose

   express Dcp2 FL-H1 at endogenous level in S.cerevisiae

   Depositor

   Michael Rosen

   Article

   Xing et al Elife. 2020 Jun 19;9. pii: 56525. doi: 1

   Insert

   Dcp2 (DCP2 Budding Yeast)
   

   Use

   Tags

   GFP

   Expression

   Yeast

   Mutation

   The first HLM (EDQLKSYAEEQLKLLLGITKEE) is added t…

   Promoter

   DCP2 promoter

   Available since

   April 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pROS13_IDH2 #7

   Plasmid

   #166102

   Purpose

   This plasmid express a sgRNA that targets the IDH2 gene. This allows Cas9 to make a double-strand break and facilitate integration of LOV2 at position IDH2-135 by homologous recombination.

   Depositor

   Matthias Heinemann

   Article

   Chen et al bioRxiv

   Insert

   IDH2 sgRNA #7 (IDH2 Budding Yeast)
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   April 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pROS13_IDH1 #4

   Plasmid

   #166101

   Purpose

   This plasmid express a sgRNA that targets the IDH1 gene. This allows Cas9 to make a double-strand break and facilitate integration of LOV2 at position IDH1-67 by homologous recombination.

   Depositor

   Matthias Heinemann

   Article

   Chen et al bioRxiv

   Insert

   IDH1 sgRNA #4 (IDH1 Budding Yeast)
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   April 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. HCP4

   Plasmid

   #166106

   Purpose

   This plasmid encodes a Cas9 protein as well as two sgRNAs, one targets the center of Cyr1 and the other targets a non-coding region on chromosome X (location X1)

   Depositor

   Matthias Heinemann

   Insert

   Cyr1Del-sgRNA/X1-sgRNA (CYR1 Budding Yeast)
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   April 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. HCP1

   Plasmid

   #166103

   Purpose

   This plasmid encodes a Cas9 protein as well as a sgRNA that targets Msn2, which will create a double strand break and allow C-terminus tagging via homologous recombination.

   Depositor

   Matthias Heinemann

   Insert

   Msn2-sgRNA#27 (MSN2 Budding Yeast)
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   April 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. HCP2

   Plasmid

   #166104

   Purpose

   This plasmid encodes a Cas9 protein as well as a sgRNA that targets Msn2, which will create a double strand break and allow C-terminus tagging via homologous recombination

   Depositor

   Matthias Heinemann

   Insert

   Msn2-sgRNA#27 (MSN2 Budding Yeast)
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   April 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pNSU299_CPR1_1

   Plasmid

   #166074

   Purpose

   Plasmid for constituive spCas9 and tet-inducible CPR1 targeting sgRNA expression for double stranded break formation in yeast

   Depositor

   Rebecca Burgess

   Insert

   CPR1 (CPR1 Budding Yeast)
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Tet-inducible

   Available since

   April 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pNSU299_RRT8

   Plasmid

   #166080

   Purpose

   Plasmid for constituive spCas9 expression and tet-inducible expression of sgRNA binding to the promoter of RRT8 for double stranded break formation in yeast.

   Depositor

   Rebecca Burgess

   Insert

   Promoter of RRT8 (RRT8 Budding Yeast)
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Tet-inducible

   Available since

   April 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pNSU299_CPR1_2

   Plasmid

   #166071

   Purpose

   Plasmid for constituive spCas9 expression and tet-inducible expression of an sgRNA targeting an intergenic site near CPR1 for double stranded break formation in yeast.

   Depositor

   Rebecca Burgess

   Insert

   Intergenic region near CPR1
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Tet-inducible

   Available since

   April 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pRR1

    Plasmid

    #166096

    Purpose

    Gal inducible expression of Cas9 WT

    Depositor

    Rebecca Burgess

    Insert

    WT spCas9
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Gal-inducible

    Available since

    April 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pNSU299_GAL7

    Plasmid

    #166089

    Purpose

    Plasmid for constituive spCas9 and tet-inducible GAL7 targeting sgRNA expression for double stranded break formation in yeast

    Depositor

    Rebecca Burgess

    Insert

    GAL7 (GAL7 Budding Yeast)
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Tet-inducible

    Available since

    April 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pNSU299_CUP1-1

    Plasmid

    #166086

    Purpose

    Plasmid for constituive spCas9 and tet-inducible CUP1-1 targeting sgRNA expression for double stranded break formation in yeast

    Depositor

    Rebecca Burgess

    Insert

    CUP1-1 (CUP1-1 Budding Yeast)
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Tet-inducible

    Available since

    April 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pPH3

    Plasmid

    #166098

    Purpose

    Gal inducible expression of D10A-Cas9 nickase

    Depositor

    Rebecca Burgess

    Insert

    Cas9-D10A
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    spCas9-D10A

    Promoter

    Gal-inducible

    Available since

    April 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pNSU299_RPL39

    Plasmid

    #166078

    Purpose

    Plasmid for constituive spCas9 expression and tet-inducible expression of sgRNA binding to the promoter of RPL39 for double stranded break formation in yeast.

    Depositor

    Rebecca Burgess

    Insert

    Promoter of RPL39 (Overlaps with 5'UTR and first base of gene) (RPL39 Budding Yeast)
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Tet-inducible

    Available since

    April 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. HCP9

    Plasmid

    #166111

    Purpose

    This plasmid encodes a Cas9 protein as well as a sgRNA that targets the C-terminus of Hta2

    Depositor

    Matthias Heinemann

    Insert

    Hta2-sg18 (HTA2 Budding Yeast)
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    April 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. HCP3

    Plasmid

    #166105

    Purpose

    This plasmid encodes a Cas9 protein as well as a sgRNA that targets Msn2, which will create a double strand break and allow C-terminus tagging via homologous recombination

    Depositor

    Matthias Heinemann

    Insert

    Msn2-sgRNA#27 (MSN2 Budding Yeast)
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    April 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. HCP6

    Plasmid

    #166108

    Purpose

    This plasmid encodes a Cas9 protein as well as two sgRNAs, one targets the C-terminus of Cyr1 and the other targets a non-coding region on chromosome X (location X1)

    Depositor

    Matthias Heinemann

    Insert

    Cyr1CT-sgRNA/X1-sgRNA (CYR1 Budding Yeast)
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    April 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. HCP7

    Plasmid

    #166109

    Purpose

    This plasmid encodes a Cas9 protein as well as two sgRNAs, one targets the C-terminus of Whi5 and the other targets the C-terminus of Hta2.

    Depositor

    Matthias Heinemann

    Insert

    Whi5-sg106 (WHI5 Budding Yeast)
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    April 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. VP2C-GFP-MIOX only

    Plasmid

    #166684

    Purpose

    Yeast integrative plasmid for expressing mouse myo-inositol oxygenase fused to VP2C-GFP (GAL10 promoter). Contains uracil auxotrophic marker (K. lactis URA3).

    Depositor

    Claudia Vickers

    Article

    Cheah et al ACS Synth Biol. 2021 Dec 17;10(12):3251-

    Insert

    VP2C-GFP-MIOX
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    GAL10

    Available since

    April 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. GFP-MIOX only

    Plasmid

    #166683

    Purpose

    Yeast integrative plasmid for expressing mouse myo-inositol oxygenase fused to GFP (GAL10 promoter). Contains uracil auxotrophic marker (K. lactis URA3).

    Depositor

    Claudia Vickers

    Article

    Cheah et al ACS Synth Biol. 2021 Dec 17;10(12):3251-

    Insert

    GFP-MIOX
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    GAL10

    Available since

    April 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge