8,318 results
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Plasmid#180211PurposeExpresses GAL4-fusion protein of Acanthoeca spectabilis NF-kB3 protein in yeastDepositorInsertAs-NF-kB3
TagsGAL4 (aa 1-147) fusion proteinExpressionYeastMutationaa 2-412PromoterAdhIAvailable SinceMarch 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDIV491
Plasmid#177699PurposePlasmid expressing optimized Cas9 and NAT marker. Compatible with CTG-clade yeast species.DepositorInsertsCas9
Nat
UseCRISPRTagsSV40ExpressionBacterial and YeastPromoterRNR2p (Debaryomyces hansenii ) and TEF1p (Ashbya ā¦Available SinceMarch 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDIV495
Plasmid#177702PurposePlasmid expressing optimized Cas9 and NAT marker and sgRNA targeting ADE2 locus in D. hanseniiDepositorInsertsCas9
Nat
sgRNA Targeting ADE2 locus in D.hansenii: AGCTAaGCAGATTAAtGCAT
UseCRISPRTagsSV40ExpressionBacterial and YeastPromoterRNR2p (Debaryomyces hansenii ), SRC1p (Debaryomycā¦Available SinceMarch 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDIV488
Plasmid#177696PurposePlasmid expressing optimized Cas9 and NAT marker. Compatible with CTG-clade yeast speciesDepositorInsertsCas9
Nat
UseCRISPRTagsSV40ExpressionBacterial and YeastPromoterTDH3p (Candida lusitaniae) and TEF1p (Ashbya gossā¦Available SinceMarch 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCSJ982
Plasmid#176622PurposeExpress yeast Ub-MPGLW-Fbp1-3xflag and reference flag-DHFR-ha protein, both are contolled by tetracycline mediated TDH promter, test protein expression and stability.DepositorInsertUb-MPGLW-Fbp1
TagsUbiquitin N-terminal fusion and triple flag tag Cā¦ExpressionYeastMutationChanged first 4 N-terminal amino acid residues ofā¦PromoterTDH3tc3Available SinceMarch 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
RP51A-No5'ss-NoBS-NoPseudoBS
Plasmid#100987PurposeRP51A with T7 promoter in HindIII/EcoRI sites of pUC18. No 5'ss, No BS, No pseudo BSDepositorInsertRP51A (RPS17A Budding Yeast)
ExpressionBacterial and YeastMutation5'ss mut to C/ATACCT BS mut to GTTAGTG, pseuā¦Available SinceMarch 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
Rp51a Hyperstabilized 5'SS
Plasmid#100988PurposepUC18 Hyperstabilized Rp51a 5'SS between HindIII and EcoRI; ATG/GTATGT -> AAG/GTAAGTDepositorInsertRP51A (RPS17A Budding Yeast)
ExpressionBacterial and YeastMutation5'ss mut ATG/GTATGT -> AAG/GTAAGTAvailable SinceMarch 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
Rp51a_10bp 5'SS_No BP or pBP_pUC18
Plasmid#100989PurposeRp51a with a tGGTAtGTta->AGGTAAGTAT 5'SS mutant with potential to form 10bps with the U1 snRNA. Also contains TACTAAC->GTTAGTG BP mutataion and a TACAAAC->GTTTGTG pseudo BP mutationDepositorInsertRP51A (RPS17A Budding Yeast)
ExpressionBacterial and YeastMutationtGGTAtGTta->AGGTAAGTAT 5'SS mutant, TACTAā¦Available SinceMarch 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
Rp51a_SUS1 5'ss_No_BP_pBP-pUC18
Plasmid#100990PurposeContains model Rp51a with a GGTAtGT->tGTAtGa 5'SS mutant (SUS1 5'SS sequence). Also contains TACTAAC->GTTAGTG BP mutataion and a TACAAAC->GTTTGTG pseudo BP mutationDepositorInsertRP51A (RPS17A Budding Yeast)
ExpressionBacterial and YeastMutationGGTAtGT->tGTAtGa 5'SS mutant, TACTAAC->ā¦Available SinceMarch 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
Rp51a_SUS1 5'SS
Plasmid#100991PurposeContains model Rp51a with a GGTAtGT->tGTAtGa 5'SS mutant (SUS1 5'SS sequence).DepositorInsertRP51A (RPS17A Budding Yeast)
ExpressionBacterial and YeastMutationGGTAtGT->tGTAtGa 5'SS mutantAvailable SinceMarch 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
Rp51a_No_BP-pUC18
Plasmid#100992PurposeContains model Rp51a with a TACTAAC->GTTAGTG BP mutataionDepositorInsertRP51A (RPS17A Budding Yeast)
ExpressionBacterial and YeastMutationTACTAAC->GTTAGTG BP mutataionAvailable SinceMarch 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
RP51A-NoBS-NoPseudoBS
Plasmid#100986PurposeRP51A w/ T7 promoter in HindIII/EcoRI sites of pUC18. Has WT 5'ss but mutant BS and no pseudo BSDepositorInsertRP51A (RPS17A Budding Yeast)
ExpressionBacterial and YeastMutationBS mutated to GTTAGTG, pseudo BS mutated to GTTTGā¦Available SinceMarch 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFBP-2_6m.mutant
Plasmid#162842PurposeYeast expression of the mutant #2_6m for the RNA-based sensor of fructose-1,6-bisphosphate #2_6DepositorInsert2_6m_RNA-device
ExpressionYeastAvailable SinceFeb. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pML104-natR412
Plasmid#162042PurposeExpresses Cas9 and contains natR412 guide which targets the natR geneDepositorInsertnatR412 guide
UseCRISPRExpressionYeastAvailable SinceFeb. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pML107-natR854
Plasmid#162043PurposeExpresses Cas9 and contains natR854 guide which targets the natR geneDepositorInsertnatR854 guide
UseCRISPRExpressionYeastAvailable SinceFeb. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDN513
Plasmid#175365PurposeYeast shuttle plasmid that allows conversion between low copy, high copy, and integrating vectors. Low copy, HIS3-selected.DepositorTypeEmpty backboneExpressionYeastAvailable SinceFeb. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDN514
Plasmid#175366PurposeYeast shuttle plasmid that allows conversion between low copy, high copy, and integrating vectors. Low copy, TRP1-selected.DepositorTypeEmpty backboneExpressionYeastAvailable SinceFeb. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDN515
Plasmid#175367PurposeYeast shuttle plasmid that allows conversion between low copy, high copy, and integrating vectors. Low copy, LEU2-selected.DepositorTypeEmpty backboneExpressionYeastAvailable SinceFeb. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDN516
Plasmid#175368PurposeYeast shuttle plasmid that allows conversion between low copy, high copy, and integrating vectors. Low copy, URA3-selected.DepositorTypeEmpty backboneExpressionYeastAvailable SinceFeb. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDN523
Plasmid#175369PurposeYeast shuttle plasmid that allows conversion between low copy, high copy, and integrating vectors. High copy, HIS3-selected.DepositorTypeEmpty backboneExpressionYeastAvailable SinceFeb. 3, 2022AvailabilityAcademic Institutions and Nonprofits only