8,126 results
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Plasmid#159623PurposeAn empty HIS3 plasmid for translation factor eIF4E complementation test in yeast, strain Jo55. Golden Gate cloning using BsaI. Selection with RFP after cloning.DepositorTypeEmpty backboneExpressionBacterial and YeastAvailable SinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only
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pJo55-CA1
Plasmid#159624PurposePlasmid pJo55, which contains positive control (C. annuum eIF4E-1) for complementation test in Jo55 yeasts.DepositorInsertC. annuum eIF4E-1
ExpressionBacterial and YeastAvailable SinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pIFU002
Plasmid#174299PurposeEncodes the SARS-CoV-2 Spike Receptor Binding Domain (RBD) with the N501Y mutation and can be combined with pJS697 (Addgene plasmid # 168778) through homologous recombinationDepositorInsertSpike RBD N343Q, N501Y (S Severe acute respiratory syndrome coronavirus 2)
TagsHA and cMycExpressionYeastMutationN343Q, N501YAvailable SinceSept. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pIFU001
Plasmid#174298PurposeEncodes the SARS-CoV-2 Spike Receptor Binding Domain (RBD) with the E484K mutation and can be combined with pJS697 (Addgene plasmid # 168778) through homologous recombinationDepositorInsertSpike RBD N343Q, E484K (S Severe acute respiratory syndrome coronavirus 2)
TagsHA and cMycExpressionYeastMutationN343Q, E484KAvailable SinceSept. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJL1
Plasmid#174070PurposeExpresses VP16-CIB1 in S. cerevisiae. Part of optogenetic system.DepositorInsertpGal-Olac-3xNLS-VP16-CIB1-tADH1
TagsFLAGExpressionYeastPromoterpGal1Available SinceSept. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJL29
Plasmid#174063PurposeExpresses for mCherry for S. cerevisiae. Contain 2 43-8 zinc-finger operators upstream of Cyc1 promoter.DepositorInsert2x43-8 ZFO-1x97-4 ZFO pCyc mCherry tCyc
TagsFLAGExpressionYeastPromoterpCyc1Available SinceSept. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJL15
Plasmid#174069PurposeExpresses 43-8 zinc finger array fused to CRY2 in S. cerevisiae. Part of optogenetic system.DepositorInsertpGAL-tetO-3xNLS-43-8WT-GS-Cry2-Tadh1
TagsFLAGExpressionYeastPromoterpGal1Available SinceSept. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRS41K-mScarlet-I-mNeonGreen-ADH1term-TEFpr-hphΔC (pKBJ011)
Plasmid#173462PurposeC-SWAT donor for Selection Reconstitution tagging, contains mScarletI-mNeonGreen tandem fluorescent protein timerDepositorInsertmScarlet-I-mNeonGreen
ExpressionYeastPromoterno promoterAvailable SinceSept. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRS41K-mCherry-mNeonGreen-ADH1term-TEFpr-hphΔC (pJJF004)
Plasmid#173460PurposeC-SWAT donor for Selection Reconstitution tagging, contains mCherry-mNeonGreen tandem fluorescent protein timerDepositorInsertmCherry-mNeonGreen
ExpressionYeastPromoterno promoterAvailable SinceSept. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRS41K-mCherry-mNeonGreen (pJJF003)
Plasmid#173459PurposeC-SWAT donor for Seamless tagging, contains mCherry-mNeonGreen tandem fluorescent protein timerDepositorInsertmCherry-mNeonGreen
ExpressionYeastPromoterno promoterAvailable SinceSept. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRS41K-mCherry-sfGFP-ADH1term-TEFpr-hphΔC (pJJF002)
Plasmid#173458PurposeC-SWAT donor for Selection Reconstitution tagging, contains mCherry-sfGFP tandem fluorescent protein timerDepositorInsertmCherry-sfGFP
ExpressionYeastPromoterno promoterAvailable SinceSept. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDB4953
Plasmid#174020PurposepDUAL plasmid expressing GFP-Atg6 from 41nmt1 promoterDepositorInsertAtg6
TagsGFP, Atg6ExpressionYeastPromoter41nmt1Available SinceSept. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDB4658
Plasmid#174017PurposepDUAL plasmid expressing GFP-Atg8 from 41nmt1 promoterDepositorInsertAtg8
TagsGFP, Atg8ExpressionYeastPromoter41nmt1Available SinceSept. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDB4958
Plasmid#174014PurposepHIS3H plasmid expressing CFP-Atg6 from 41nmt1 promoterDepositorInsertAtg6
TagsCFP, Atg6ExpressionYeastPromoter41nmt1Available SinceSept. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDB4924
Plasmid#174012PurposepDUAL plasmid expressing Pil1-mCherry from 41nmt1 promoterDepositorInsertPil1-mCherry
TagsPil1, mCherryExpressionYeastPromoter41nmt1 and 41nmt1pAvailable SinceSept. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDB4948
Plasmid#174019PurposepDUAL plasmid expressing Pil1-mCherry-Vps38 from 41nmt1 promoterDepositorInsertVps38
TagsPil1, mCherry, Vps38ExpressionYeastPromoter41nmt1Available SinceSept. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDB4946
Plasmid#174018PurposepDUAL plasmid expressing Pil1-mCherry-Atg14 from 41nmt1 promoterDepositorAvailable SinceSept. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDB4925
Plasmid#174016PurposepDUAL plasmid expressing Pil1-mCherry-Atg38(161-190) from 41nmt1 promoterDepositorInsertAtg38(161-190)
TagsPil1, mCherry, Atg38(161-190)ExpressionYeastPromoter41nmt1Available SinceSept. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJS697
Plasmid#168778PurposeYeast surface display plasmid backbone that can be combined with pJS699 through homologous recombinationDepositorTypeEmpty backboneTagsCmyc and HAExpressionYeastAvailable SinceAug. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
U6-U57A
Plasmid#169927PurposeWT U6 with a U57A mutationDepositorInsertU6 U57A
ExpressionBacterial and YeastAvailable SinceAug. 5, 2021AvailabilityAcademic Institutions and Nonprofits only