8,060 results

1. pJPS2957

   Plasmid

   #135591

   Purpose

   SNR6 has GAGAUUUAUUUC at position 96–107 deleted

   Depositor

   Jonathan Staley

   Article

   Toroney et al Genes Dev. 2019 Sep 26. pii: gad.328294.

   Insert

   snR6 (snR6 Budding Yeast)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   U6Δ12 has GAGAUUUAUUUC at position 96–107 deleted

   Promoter

   Native

   Available since

   Dec. 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pPSG569

   Plasmid

   #158588

   Purpose

   tNCS-ePTS1 Cen6/ARS4

   Depositor

   John Dueber

   Article

   Grewal et al Nat Chem Biol. 2020 Oct 12. pii: 10.1038

   Insert

   tNCS-ePTS1
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Dec. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pMJB1-RPL1B-Cl

   Plasmid

   #160430

   Purpose

   Expresses "cre-less" yeast RPL1B tagged with mCherry under the GAL1 promoter

   Depositor

   Gary Stormo

   Article

   Roy et al Commun Biol. 2020 Dec 11;3(1):761. doi:

   Insert

   RPL1B
   

   Use

   Tags

   mCherry

   Expression

   Yeast

   Mutation

   Removed introns, shuffled codons to vary nucleoti…

   Promoter

   S. cerevisiae GAL1

   Available since

   Nov. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. plIVMD217

   Plasmid

   #160939

   Purpose

   Excess Capsid Protein in vivo mRNA display

   Depositor

   Saeed Tavazoie

   Article

   Oikonomou et al Proc Natl Acad Sci U S A. 2020 Oct 9. pi

   Insert

   MCP-mTagBFP2-HA Tag
   

   Use

   Tags

   HA tag

   Expression

   Yeast

   Mutation

   Promoter

   MET25

   Available since

   Nov. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. plIVMD118

   Plasmid

   #160938

   Purpose

   Excess Capsid Protein in vivo mRNA display

   Depositor

   Saeed Tavazoie

   Article

   Oikonomou et al Proc Natl Acad Sci U S A. 2020 Oct 9. pi

   Insert

   MCP-mCherry-FLAG tag
   

   Use

   Tags

   MYC tag

   Expression

   Yeast

   Mutation

   Promoter

   MET25

   Available since

   Nov. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. plIVMD115

   Plasmid

   #160937

   Purpose

   Excess Capsid Protein for in vivo mRNA display

   Depositor

   Saeed Tavazoie

   Article

   Oikonomou et al Proc Natl Acad Sci U S A. 2020 Oct 9. pi

   Insert

   MCP-mCherry-MYC tag
   

   Use

   Tags

   MYC tag

   Expression

   Yeast

   Mutation

   Promoter

   MET25

   Available since

   Nov. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. plIVMD163

   Plasmid

   #160935

   Purpose

   In vivo mRNA display construct for mCherry

   Depositor

   Saeed Tavazoie

   Article

   Oikonomou et al Proc Natl Acad Sci U S A. 2020 Oct 9. pi

   Insert

   MCP-mCherry-HIS tag, 3'UTR MS2 Stem Loop
   

   Use

   Tags

   HIS tag, 3'UTR MS2 Stem Loop

   Expression

   Yeast

   Mutation

   Promoter

   MET25

   Available since

   Nov. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pAM1160c2

   Plasmid

   #159724

   Purpose

   Express MPGIL-G6-Dhfr in yeast two hybrid plasmid, pGBKCg

   Depositor

   Alexander Varshavsky

   Article

   Melnykov et al Proc Natl Acad Sci U S A. 2019 Aug 6;116

   Insert

   mDHFR (Dhfr Mouse)
   

   Use

   Tags

   Gal4DBD and MPGIL-GGGGGG

   Expression

   Yeast

   Mutation

   remove start and stop codon of Dhfr

   Promoter

   ADH1

   Available since

   Nov. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pPICZ-aC-VTS-US

   Plasmid

   #160425

   Purpose

   Expression of c-luciferase from sp. Vargula tsujii (USA)

   Depositor

   Todd Oakley

   Article

   Hensley et al Mol Ecol. 2020 Oct 8. doi: 10.1111/mec.1

   Insert

   luciferase
   

   Use

   Luciferase

   Tags

   6x Histidine tag, Yeast alpha secretion factor, c…

   Expression

   Yeast

   Mutation

   See depositor comments below

   Promoter

   AOX

   Available since

   Oct. 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pPICZ-aC-MSVU-BZ

    Plasmid

    #160426

    Purpose

    Expression of c-luciferase from sp. Maristella sp. SVU (Belize)

    Depositor

    Todd Oakley

    Article

    Hensley et al Mol Ecol. 2020 Oct 8. doi: 10.1111/mec.1

    Insert

    luciferase
    

    Use

    Luciferase

    Tags

    6x Histidine tag, Yeast alpha secretion factor, c…

    Expression

    Yeast

    Mutation

    See depositor comments below

    Promoter

    AOX

    Available since

    Oct. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pPSG1059

    Plasmid

    #158595

    Purpose

    tNCS-ePTS1, 6OMT, 4'OMT2, CNMT (CEN6/ARS4)

    Depositor

    John Dueber

    Article

    Grewal et al Nat Chem Biol. 2020 Oct 12. pii: 10.1038

    Inserts

    tNCS-ePTS1
    Ec_CYP80B1
    Ps_6OMT
    Ps_4'OMT2
    Ps_CNMT
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pPSG1060

    Plasmid

    #158596

    Purpose

    tNCS-dead-ePTS1, 6OMT, 4'OMT2, CNMT (CEN6/ARS4)

    Depositor

    John Dueber

    Article

    Grewal et al Nat Chem Biol. 2020 Oct 12. pii: 10.1038

    Inserts

    tNCS-dead_ePTS1
    Ec_CYP80B1
    Ps_6OMT
    Ps_4'OMT2
    Ps_CNMT
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pPSG1067

    Plasmid

    #158597

    Purpose

    tNCS-Venus (CEN6/ARS4)

    Depositor

    John Dueber

    Article

    Grewal et al Nat Chem Biol. 2020 Oct 12. pii: 10.1038

    Insert

    tNCS-Venus-ePTS1
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pJJB207

    Plasmid

    #158599

    Purpose

    UbiY-Venus-ePTS1

    Depositor

    John Dueber

    Article

    Grewal et al Nat Chem Biol. 2020 Oct 12. pii: 10.1038

    Insert

    UbiY-Venus-ePTS1
    

    Use

    Tags

    Ubi-Y and ePTS1

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pPSG1066

    Plasmid

    #158590

    Purpose

    tNCS-Venus-ePTS1 CEN6/ARS4

    Depositor

    John Dueber

    Article

    Grewal et al Nat Chem Biol. 2020 Oct 12. pii: 10.1038

    Insert

    tNCS-Venus-ePTS1
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pJAS1643

    Plasmid

    #158591

    Purpose

    tNCS-VioE CEN6/ARS4

    Depositor

    John Dueber

    Article

    Grewal et al Nat Chem Biol. 2020 Oct 12. pii: 10.1038

    Insert

    tNCS-VioE-ePTS1
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pJAS1644

    Plasmid

    #158592

    Purpose

    tNCS-VioE-dead_ePTS1 CEN6/ARS4

    Depositor

    John Dueber

    Article

    Grewal et al Nat Chem Biol. 2020 Oct 12. pii: 10.1038

    Insert

    tNCS-VioE-dead_ePTS1
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pDUAL-Pnmt1-Ost4-GFP

    Plasmid

    #154368

    Purpose

    Yeast expression of Ost4

    Depositor

    Wanzhong He

    Article

    Jiang et al Nat Methods. 2020 Sep;17(9):937-946. doi

    Insert

    Ost4 (ost4 Fission Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pPEE45

    Plasmid

    #139424

    Purpose

    Recyclable marker for use in Cryptococcus neoformans. Contains the amdS2 counterselectable marker flanked by the mTurquoise2 ORF and a flexible linker at the 5' end.

    Depositor

    James Fraser

    Article

    Spencer et al Fungal Genet Biol. 2020 May;138:103365.

    Insert

    mTurquoise1
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    TEF1

    Available since

    Oct. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pPEE44

    Plasmid

    #139423

    Purpose

    Recyclable marker for use in Cryptococcus neoformans. Contains the amdS2 counterselectable marker flanked by the mTFP1 ORF and a flexible linker at the 5' end.

    Depositor

    James Fraser

    Article

    Spencer et al Fungal Genet Biol. 2020 May;138:103365.

    Insert

    mTFP1
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    TEF1

    Available since

    Oct. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge