8,436 results
-
Plasmid#165061PurposeIntegrative expression of SKP1-fusing rice auxin receptor gene OsTIR1DepositorInsertP-URA3>KlURA3>T-AgTEF1- T-PGK1-P-ACS2> SKP1>OsTIR1opt>T-URA3
ExpressionYeastMutationWTAvailable sinceMarch 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pILGFP105
Plasmid#165060PurposeIntegrative expression of nucleus-targeting yEGFP-AID*(mini auxin inducible degron)-CUP1DepositorInsertP-URA3>KlURA3>T-AgTEF1-P-TEF1>yEGFP>AID*>CUP1>SV40NSL>T-PGK1- T-URA3
ExpressionYeastMutationWTAvailable sinceMarch 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pILGFP104
Plasmid#165059PurposeIntegrative expression of yEGFP-AID*(mini auxin inducible degron)-CUP1DepositorInsertP-URA3>KlURA3>T-AgTEF1-P-TEF1>yEGFP>AID*>CUP1>T-PGK1-T-URA3
ExpressionYeastMutationWTAvailable sinceMarch 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJAID8Z105
Plasmid#165057PurposeIntegrative expression of nucleus-targeting yEGFP-AID*-CUP1 and SKP1-fusing rice auxin receptor gene OsTIR1.DepositorInsertP-URA3>KlURA3>T-AgTEF1-P-TEF1>yEGFP>AID*>CUP1>SV40NSL>T-PGK1-P-ACS2>SKP1>OsTIR1opt>T-URA3
ExpressionYeastMutationWTAvailable sinceMarch 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJAID9L105
Plasmid#165055PurposeIntegrative expression of nucleus-targeting yEGFP-AID*-CUP1 and rice auxin receptor gene OsTIR1.DepositorInsertP-URA3>KlURA3>T-AgTEF1-P-TEF1>yEGFP>AID*>CUP1>SV40NSL>T-PGK1-P-ACS2>OsTIR1opt>T-URA3
ExpressionYeastMutationWTAvailable sinceMarch 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJAID9L104
Plasmid#165054PurposeIntegrative expression of yEGFP-AID*-CUP1 and rice auxin receptor gene OsTIR1.DepositorInsertP-URA3>KlURA3>T-AgTEF1-P-TEF1>yEGFP>AID*>CUP1>T-PGK1-P-ACS2>OsTIR1opt>T-URA3
ExpressionYeastMutationWTAvailable sinceMarch 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJAID9L
Plasmid#165052PurposeIntegrative expression of yEGFP and rice auxin receptor gene OsTIR1. OsTIR1 is codon-optimised for expression in S. cerevisiaeDepositorInsertP-URA3>KlURA3>T-AgTEF1-P-TEF1>yEGFP>T-PGK1-P-ACS2>OsTIR1opt>T-URA3
ExpressionYeastMutationWTAvailable sinceMarch 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJAID5Z
Plasmid#165047PurposeIntegrative expression of AID* (mini auxin inducible degron)-yEGFP and rice auxin receptor gene OsTIR1.DepositorInsertP-URA3>KlURA3>T-AgTEF1-P-TEF1>AID*>yEGFP>T-PGK1-P-ACS2>OsTIR1opt>T-URA3
ExpressionYeastMutationOsTIR1 is codon-optimised for expression in S. ce…Available sinceMarch 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
CPH3568 (YCp LEU2 Rpb1 P1455_E1456InsGGGGGLEVLFQGP(H)10,N1501K; msLink1)
Plasmid#91819PurposeYeast expression of S. cerevisiae Rpb1 with an internal PreScission protease site and 10xHis tag for purification of the linker-CTDDepositorInsertRPO21 (RPO21 Budding Yeast)
Tags10xHisExpressionYeastMutation5xGly linker, PreScission Protease site (LEVLFQGP…PromoterEndogenous Rpb1 promoterAvailable sinceMarch 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
CPH3569 (YCp LEU2 Rpb1 P1455_E1456InsGGGGGLEVLFQGP(H)10,K1487N,D1497K; msLink2)
Plasmid#91820PurposeYeast expression of S. cerevisiae Rpb1 with an internal PreScission protease site and 10xHis tag for purification of the linker-CTDDepositorInsertRPO21 (RPO21 Budding Yeast)
Tags10xHisExpressionYeastMutation5xGly linker, PreScission Protease site (LEVLFQGP…PromoterEndogenous Rpb1 promoterAvailable sinceMarch 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
CPH3400 (YCp LEU2 Rpb1 A1529_G1534delInsLEVLFQGP)
Plasmid#91806PurposeYeast expression of S. cerevisiae Rpb1 with an internal PreScission protease siteDepositorInsertRPO21 (RPO21 Budding Yeast)
ExpressionYeastMutationResidues A1529-G1534 replaced with a PreScission …PromoterEndogenous Rpb1 promoterAvailable sinceMarch 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
CPH3477 (YCp LEU2 Rpb1 P1455_E1456InsLEVLFQGP)
Plasmid#91807PurposeYeast expression of S. cerevisiae Rpb1 with an internal PreScission protease siteDepositorInsertRPO21 (RPO21 Budding Yeast)
ExpressionYeastMutationPreScission Protease site (LEVLFQGP) inserted bet…PromoterEndogenous Rpb1 promoterAvailable sinceMarch 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
CPH3486 (YCp LEU2 Rpb1 F1492A)
Plasmid#91808PurposeYeast expression of S. cerevisiae Rpb1 with a F1492A mutationDepositorInsertRPO21 (RPO21 Budding Yeast)
ExpressionYeastMutationchanged phenylalanine 1492 to alaninePromoterEndogenous Rpb1 promoterAvailable sinceMarch 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pVG50
Plasmid#164708PurposeyEVenus under the control of tetOpr with ADH1t upstream of the reporterDepositorInsertADH1t - tetOpr - yEVenus - CLN2 PEST - ADH1t
ExpressionYeastAvailable sinceMarch 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pVG88
Plasmid#164709PurposeyEVenus under the control of ARG3 promoterDepositorInsertARG3pr - yEVenus - CLN2 PEST - ADH1t
ExpressionYeastAvailable sinceMarch 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
YIplac211-KAR2-mEGFP-HDEL
Plasmid#160467PurposeYeast integration vector for the gene replacement of S. cerevisiae KAR2 fused to mEGFP with an ER retrieval signalDepositorInsertKAR2-mEGFP-HDEL
ExpressionYeastAvailable sinceMarch 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCL10
Plasmid#164710PurposeyEVenus under the control of MET3 promoterDepositorInsertMET3pr - yEVenus - CLN2 PEST - ADH1t
ExpressionYeastAvailable sinceMarch 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUC19-PpSEC13-mEGFP
Plasmid#160469PurposeP. pastoris integration vector for the gene replacement of SEC13 fused to mEGFPDepositorInsertppSEC13-mEGFP
ExpressionYeastAvailable sinceMarch 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUC19-PpSEC13-msGFP2
Plasmid#160470PurposeP. pastoris integration vector for the gene replacement of SEC13 fused to msGFP2DepositorInsertppSEC13-msGFP2
ExpressionYeastAvailable sinceMarch 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
Dcp2 ΔN
Plasmid#163575Purposeexpress wild type Dcp2 ΔN(243-970) at endogenous level in S.cerevisiaeDepositorInsertDcp2 (DCP2 Budding Yeast)
TagsGFPExpressionYeastMutationTruncation of N-terminal domain (1-242) of Dcp2PromoterDCP2 promoterAvailable sinceMarch 2, 2021AvailabilityAcademic Institutions and Nonprofits only