8,060 results

1. pJK272

   Plasmid

   #71562

   Purpose

   Produces Crepis alpina delta12 fatty acid acetylenase (vFAD2) in yeast

   Depositor

   T. J. Kappock

   Article

   Nam et al J Exp Bot. 2007;58(6):1421-32. Epub 2007

   Insert

   delta12 fatty acid acetylenase
   

   Use

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   GAL1

   Available Since

   Jan. 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pDS3-aurJ

   Plasmid

   #70152

   Purpose

   Fusarium graminearum AurJ encoded on CEN/ARS yeast shuttle vector with DS3 selection marker

   Depositor

   Morten Sommer

   Article

   Rugbjerg et al Metab Eng. 2015 Sep;31:189-97. doi: 10.1

   Insert

   aurJ
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   GPD1

   Available Since

   Dec. 23, 2015

   Availability

   Academic Institutions and Nonprofits only

3. pDS3-X4

   Plasmid

   #70140

   Purpose

   Yeast chr. integration vector with DS3 selection marker

   Depositor

   Morten Sommer

   Article

   Rugbjerg et al Metab Eng. 2015 Sep;31:189-97. doi: 10.1

   Insert

   DS3
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   AgTEF1

   Available Since

   Dec. 23, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pRS425-M25-Afg1

   Plasmid

   #61720

   Purpose

   yeast Afg1 AAA protein in yeast expression vector

   Depositor

   Oleh Khalimonchuk

   Article

   Germany et al J Cell Sci. 2018 Nov 21;131(22). pii: jc

   Insert

   Afg1 (AFG1 Budding Yeast)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   Dec. 22, 2015

   Availability

   Academic Institutions and Nonprofits only

5. pRS415-Cox15-13xMyc

   Plasmid

   #61721

   Purpose

   yeast Cox15 heme A synthase with C-terminal 13xMyc tag in low-copy yeast expression vector

   Depositor

   Oleh Khalimonchuk

   Article

   Swenson et al J Biol Chem. 2016 Mar 3. pii: jbc.M115.7

   Insert

   Cox15 (COX15 Budding Yeast)
   

   Use

   Tags

   13xMyc

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   Dec. 22, 2015

   Availability

   Academic Institutions and Nonprofits only

6. pCfB2229

   Plasmid

   #67537

   Purpose

   EasyClone system-based yeast integrative vector carrying loxP-flanked CaLYS5 marker, integration into S. cerevisiae XI-5 chromosomal location, USER site for cloning, amp resistance

   Depositor

   Irina Borodina

   Article

   Stovicek et al J Ind Microbiol Biotechnol. 2015 Sep 16.

   Type

   Empty backbone

   Use

   Cre/Lox; Integrative

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   Nov. 3, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. LD123

   Plasmid

   #69173

   Purpose

   Integration of GFP tag at Atg23 C terminus, use auxotrophic marker URA3(Candida albicans)

   Depositor

   Zhiping Xie

   Article

   Li et al Autophagy. 2015;11(6):954-60. doi: 10.10

   Insert

   Atg23-EGFP
   

   Use

   Tags

   EGFP

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   Oct. 28, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. LD114

   Plasmid

   #69167

   Purpose

   Integration of GFP tag at Atg14 C terminus, use auxotrophic marker URA3(Candida albicans)

   Depositor

   Zhiping Xie

   Article

   Li et al Autophagy. 2015;11(6):954-60. doi: 10.10

   Insert

   Atg14-EGFP
   

   Use

   Tags

   EGFP

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   Oct. 28, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. LD105

   Plasmid

   #69162

   Purpose

   Integration of GFP tag at Atg5 C terminus, use auxotrophic marker URA3(Candida albicans)

   Depositor

   Zhiping Xie

   Article

   Li et al Autophagy. 2015;11(6):954-60. doi: 10.10

   Insert

   Atg5-EGFP
   

   Use

   Tags

   EGFP

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   Oct. 28, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. BS-Trp1

    Plasmid

    #69196

    Purpose

    complementation of yeast auxotrophic marker TRP1. Although this is the plasmid described in the paper, the depositor notes that it has a low integration efficiency and Plasmid 69503 should be used.

    Depositor

    Zhiping Xie

    Article

    Li et al Autophagy. 2015;11(6):954-60. doi: 10.10

    Insert

    TRP1
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Oct. 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCyc-Bem1

    Plasmid

    #66573

    Purpose

    Express an additional copy of Bem1, integrated at TRP locus

    Depositor

    Orion Weiner

    Article

    Jost et al ACS Synth Biol. 2015 Jun 2.

    Insert

    Bem1
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    F99I*(see below)

    Promoter

    pCyc1

    Available Since

    Oct. 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. LD131

    Plasmid

    #69177

    Purpose

    Integration of GFP tag at Atg31 C terminus, use auxotrophic marker URA3(Candida albicans)

    Depositor

    Zhiping Xie

    Article

    Li et al Autophagy. 2015;11(6):954-60. doi: 10.10

    Insert

    Atg31-EGFP
    

    Use

    Tags

    EGFP

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Sept. 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. LD129

    Plasmid

    #69176

    Purpose

    Integration of GFP tag at Atg29 C terminus, use auxotrophic marker URA3(Candida albicans)

    Depositor

    Zhiping Xie

    Article

    Li et al Autophagy. 2015;11(6):954-60. doi: 10.10

    Insert

    Atg29-EGFP
    

    Use

    Tags

    EGFP

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Sept. 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. LD124

    Plasmid

    #69174

    Purpose

    Integration of GFP tag at Atg24 C terminus, use auxotrophic marker URA3(Candida albicans)

    Depositor

    Zhiping Xie

    Article

    Li et al Autophagy. 2015;11(6):954-60. doi: 10.10

    Insert

    Atg24-EGFP
    

    Use

    Tags

    EGFP

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Sept. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. LD120

    Plasmid

    #69171

    Purpose

    Integration of GFP tag at Atg20 C terminus, use auxotrophic marker URA3(Candida albicans)

    Depositor

    Zhiping Xie

    Article

    Li et al Autophagy. 2015;11(6):954-60. doi: 10.10

    Insert

    Atg20-EGFP
    

    Use

    Tags

    EGFP

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Sept. 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pED55 pRS306 pRPS2-SteND-NLS-mCherry

    Plasmid

    #68277

    Purpose

    SKARS: Fus3 and Kss1 MAPK activity sensor: Ste7ND 2xNLS mCherry Non functional sensor with non-docking mutation

    Depositor

    Serge Pelet

    Article

    Durandau et al BMC Biol. 2015 Aug 1;13:55. doi: 10.1186

    Insert

    Ste7ND NLS mCherry
    

    Use

    Tags

    mCherry

    Expression

    Yeast

    Mutation

    Promoter

    pRPS2

    Available Since

    Sept. 14, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pED53 pRS306 pRPS2-Ste7DS NLS(4A)-mCherry

    Plasmid

    #68275

    Purpose

    SKARS: Fus3 and Kss1 MAPK activity sensor: Ste7DS 2xNLS(4A) mCherry Non functional sensor with non-phosphorylatable NLS

    Depositor

    Serge Pelet

    Article

    Durandau et al BMC Biol. 2015 Aug 1;13:55. doi: 10.1186

    Insert

    Ste7DS NLS(4A) mCherry
    

    Use

    Tags

    mCherry

    Expression

    Yeast

    Mutation

    Promoter

    pRPS2

    Available Since

    Sept. 14, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCyc-Cdc24

    Plasmid

    #66574

    Purpose

    Express an additional copy of Cdc24, integrated at TRP locus

    Depositor

    Orion Weiner

    Article

    Jost et al ACS Synth Biol. 2015 Jun 2.

    Insert

    Cdc24
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    pCyc1

    Available Since

    July 14, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pRPR1_g2gRNA_RPR1t

    Plasmid

    #64388

    Purpose

    encodes g2 gRNA

    Depositor

    Timothy Lu

    Article

    Farzadfard et al ACS Synth Biol. 2013 Sep 11.

    Insert

    g2 gRNA
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    pRPR1

    Available Since

    May 19, 2015

    Availability

    Academic Institutions and Nonprofits only

20. pRS424-TUB2 - human TUBB3 Cterminal tail delta K

    Plasmid

    #60404

    Purpose

    Expression of chimeric yeast beta tubulin (TUB2) - human beta3 tubulin Cterminal tail (TUBB3) without last 4 amino acids--QGPK

    Depositor

    Ron Vale

    Article

    Sirajuddin et al Nat Cell Biol. 2014 Apr;16(4):335-44. do

    Insert

    TUB2
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    TUB2 amino acids 429 - end, replaced with human T…

    Promoter

    GAL

    Available Since

    May 6, 2015

    Availability

    Academic Institutions and Nonprofits only