8,126 results

1. pNB649

   Plasmid

   #60136

   Purpose

   Firefly luciferase (Promega) driven by MET17 promoter.

   Depositor

   Nicolas Buchler

   Article

   Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

   Insert

   FLuc
   

   Expression

   Yeast

   Mutation

   A4V

   Promoter

   MET17pr

   Available Since

   Nov. 18, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. UASGAL-CU1-Pleum

   Plasmid

   #60331

   Purpose

   Contains a promoter comprised of derivatives from native promoters galactose, cyc and leucine.

   Depositor

   Hal Alper

   Article

   Blazeck et al Biotechnol Bioeng. 2012 Nov;109(11):2884

   Insert

   promoter
   

   Use

   Synthetic Biology; Yeast synthetic hybrid promote…

   Expression

   Yeast

   Available Since

   Nov. 6, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

3. pNB689

   Plasmid

   #60133

   Purpose

   Green beetle luciferase with nuclear localization signal (NLS) and ClpXP degron tag (ssrA), driven by MET17 promoter.

   Depositor

   Nicolas Buchler

   Article

   Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

   Insert

   GrLuc
   

   Tags

   ClpXP degron and Nuclear localization signal (NLS…

   Expression

   Yeast

   Promoter

   MET17pr

   Available Since

   Oct. 31, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pNB756

   Plasmid

   #60149

   Purpose

   Yellow beetle luciferase with nuclear localization signal (NLS) and ClpXP degron tag (ssrA), driven by LEU1 promoter.

   Depositor

   Nicolas Buchler

   Article

   Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

   Insert

   YeLuc
   

   Tags

   ClpXP degron and Nuclear localization signal (NLS…

   Expression

   Yeast

   Promoter

   LEU1pr

   Available Since

   Oct. 31, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pNB752

   Plasmid

   #60155

   Purpose

   Green beetle luciferase with nuclear localization signal (NLS) and adjustable degron tag (ssrA), driven by ADE17 promoter.

   Depositor

   Nicolas Buchler

   Article

   Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

   Insert

   GrLuc
   

   Tags

   ClpXP degron and Nuclear localization signal (NLS…

   Expression

   Yeast

   Promoter

   ADE17pr

   Available Since

   Oct. 31, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pNB812

   Plasmid

   #60160

   Purpose

   Yeast codon-optimized yellow fluorescent protein (yEVenus) with PEST degron driven by RNR1 promoter.

   Depositor

   Nicolas Buchler

   Article

   Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

   Insert

   yEVenus-PEST
   

   Tags

   PEST degron

   Expression

   Yeast

   Promoter

   RNR1pr

   Available Since

   Oct. 31, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMZ286

   Plasmid

   #52227

   Purpose

   Expression of sgRNA precursor targeted against ade6L469

   Depositor

   Mikel Zaratiegui

   Article

   Jacobs et al Nat Commun. 2014 Oct 29;5:5344. doi: 10.

   Insert

   rrk1-sgRNA
   

   Use

   CRISPR

   Expression

   Yeast

   Promoter

   rrk1

   Available Since

   Oct. 29, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. p41Nat 3-F GW

   Plasmid

   #58553

   Purpose

   CEN vector with clonNat resistance marker for triple Gateway insertion

   Depositor

   Leonid Kruglyak

   Type

   Empty backbone

   Expression

   Yeast

   Available Since

   Oct. 24, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. p416GAL1-Skn7t-L-IFP-F[1] (Yeast)

   Plasmid

   #52896

   Purpose

   Skn7t replaces the zipper and is fused to the IFP-F[1] through the linker (GGGGS) X 2. Plasmid confers ampicillin resistance to DH5α.

   Depositor

   Stephen Michnick

   Article

   Tchekanda et al Nat Methods. 2014 Jun;11(6):641-4. doi:

   Insert

   Skn7t-L-IFP-F[1]
   

   Tags

   L-IFP-F[1]

   Expression

   Yeast

   Promoter

   GAL1

   Available Since

   June 12, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. p413GAL1-Ypd*1-L-IFP-F[2] (Yeast)

    Plasmid

    #52898

    Purpose

    YpdI* is fused to the IFP-F[2] by (GGGGS) X 2. Ypd1*/Skn7t: no signal, Ypd1*/Ssk1t: weak signal. Internal ClaI site in Ypd*I. Plasmid confers ampicillin resistance to DH5α.

    Depositor

    Stephen Michnick

    Article

    Tchekanda et al Nat Methods. 2014 Jun;11(6):641-4. doi:

    Insert

    Ypd*1-L-IFP-F[2]
    

    Tags

    L-IFP-F[2]

    Expression

    Yeast

    Mutation

    Changed Tryptophane 80 to Alanine

    Promoter

    GAL1

    Available Since

    June 12, 2014

    Availability

    Academic Institutions and Nonprofits only

11. pDL86

    Plasmid

    #43909

    Depositor

    Stephen Elledge

    Article

    Lee et al Mol Cell. 2008 Oct 10;32(1):70-80. doi:

    Insert

    DIF1 (DIF1 Budding Yeast)
    

    Expression

    Yeast

    Available Since

    June 10, 2014

    Availability

    Academic Institutions and Nonprofits only

12. pGLx2-set1-H1017K

    Plasmid

    #53258

    Purpose

    GAL driven LexA-tagged SET1-catalytically dead (H1017K)

    Depositor

    Jean Cook

    Article

    Rizzardi et al Genetics. 2012 Oct;192(2):371-84. doi: 1

    Insert

    SET1
    

    Tags

    LexA

    Expression

    Yeast

    Mutation

    H1017K mutation - catalytically dead

    Promoter

    GAL1/10

    Available Since

    June 9, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. pZHY566

    Plasmid

    #50608

    Purpose

    TALEN expression vector for yeast, N152,C18 architecture

    Depositor

    Daniel Voytas

    Article

    Zhang et al Plant Physiol. 2012 Nov 2.

    Type

    Empty backbone

    Use

    TALEN

    Expression

    Yeast

    Promoter

    TEF

    Available Since

    April 16, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pZHY565

    Plasmid

    #50607

    Purpose

    TALEN expression vector for yeast, N152,C18 architecture

    Depositor

    Daniel Voytas

    Article

    Zhang et al Plant Physiol. 2012 Nov 2.

    Type

    Empty backbone

    Use

    TALEN

    Expression

    Yeast

    Promoter

    TEF

    Available Since

    Jan. 31, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pSS63

    Plasmid

    #47951

    Purpose

    rtTA positive feedback loop with single tet operator in the GAL1 promoter

    Depositor

    Attila Becskei

    Article

    Hsu et al Nat Commun. 2012 Feb 21;3:682. doi: 10.1

    Insert

    rtTA
    

    Use

    Tet inducible expression

    Expression

    Yeast

    Promoter

    T3

    Available Since

    Nov. 14, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pFA6a-link-yomEmerald-CaURA3

    Plasmid

    #44890

    Purpose

    Yeast tagging vector to create gene fusion with yeast optimized mEmerald with CaUra3 selection

    Depositor

    Wendell Lim, Kurt Thorn

    Article

    Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

    Insert

    yomEmerald
    

    Expression

    Yeast

    Available Since

    Nov. 7, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAAH4

    Plasmid

    #41828

    Purpose

    Used as a PCR template for tagging yeast proteins with the DHFR tag by homologous recombination and selection for phleomycin resistance

    Depositor

    Melissa Moore

    Article

    Hoskins et al Science. 2011 Mar 11;331(6022):1289-95.

    Type

    Empty backbone

    Use

    Pcr template

    Expression

    Yeast

    Promoter

    TEF

    Available Since

    Oct. 29, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCM305

    Plasmid

    #44550

    Depositor

    Michael Grunstein

    Article

    Millar et al Genes Dev. 2006 Mar 15;20(6):711-22.

    Insert

    HA-htz1
    

    Expression

    Yeast

    Available Since

    Oct. 23, 2013

    Availability

    Academic Institutions and Nonprofits only

19. pFA6a-link-PhiYellow-SpHis5

    Plasmid

    #44651

    Purpose

    Yeast tagging vector to create gene fusion with PhiYellow with SpHis5 selection

    Depositor

    Kurt Thorn

    Insert

    phiYellow
    

    Expression

    Yeast

    Available Since

    Oct. 16, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pDR-AmTrac-GS

    Plasmid

    #47769

    Purpose

    expresses AmTrac-GS in yeast

    Depositor

    Wolf Frommer

    Article

    De Michele et al eLife. 2013 Jul 2;2:e00800.

    Insert

    AmTrac-GS
    

    Tags

    mcpGFP

    Expression

    Yeast

    Promoter

    PMA

    Available Since

    Sept. 17, 2013

    Availability

    Academic Institutions and Nonprofits only