8,388 results
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Plasmid#63200PurposepCACS backbone (Amp), SpHIS5, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. LoxP flanked TU. Integration into chrVI: 97873-98803 (NotI or BciVI).DepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterial and YeastAvailabilityAcademic Institutions and Nonprofits only
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pAV10.K3.loxP
Plasmid#63202PurposepCACS backbone (Amp), SpHIS5, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. LoxP flanked TU. Integration into YKL162C-A (NotI).DepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterial and YeastAvailabilityAcademic Institutions and Nonprofits only -
pAV10.K6.loxP
Plasmid#63203PurposepCACS backbone (Amp), KlURA3, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. LoxP flanked TU. Integration into YKL162C-A (NotI or BciVI).DepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterial and YeastAvailabilityAcademic Institutions and Nonprofits only -
pAV10.K5.loxP
Plasmid#63204PurposepCACS backbone (Amp), LEU2, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. LoxP flanked TU. Integration into YKL162C-A (NotI or BciVI).DepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterial and YeastAvailabilityAcademic Institutions and Nonprofits only -
pAV10.HO3.loxP
Plasmid#63205PurposepCACS backbone (Amp), SpHIS5, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. LoxP flanked TU. Integration into HO locus (NotI).DepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterial and YeastAvailabilityAcademic Institutions and Nonprofits only -
pAV10.HO5.loxP
Plasmid#63206PurposepCACS backbone (Amp), LEU2, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. LoxP flanked TU. Integration into HO locus (NotI or BciVI).DepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterial and YeastAvailabilityAcademic Institutions and Nonprofits only -
pAV10.HO6
Plasmid#63211PurposepCACS backbone (Amp), KlURA3, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. Integration into HO locus (NotI or BciVI).DepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterial and YeastAvailabilityAcademic Institutions and Nonprofits only -
L284F Cus2p
Plasmid#73059PurposeS. cerevisiae Cus2p gene with L284F MutationDepositorArticleInsertCUS2p (CUS2 Budding Yeast)
Tags6x His tag fused to TEV cleavage siteExpressionBacterial and YeastMutationLeucine 284 to PhenylalaninePromoterT7AvailabilityAcademic Institutions and Nonprofits only -
D282N Cus2p
Plasmid#73060PurposeS. cerevisiae Cus2p gene with D282N MutationDepositorArticleInsertCUS2p (CUS2 Budding Yeast)
Tags6x His tag fused to TEV cleavage siteExpressionBacterial and YeastMutationAsp 282 to AsnPromoterT7AvailabilityAcademic Institutions and Nonprofits only -
pEMY01AB-PRO
Plasmid#127714PurposeIntegrative yeast promoter characterization plasmid. Clone in with BpiI. Has ChrXV homology upstream and an mVenus fragment downstream.DepositorTypeEmpty backboneUseSynthetic BiologyExpressionYeastAvailabilityAcademic Institutions and Nonprofits only -
pDSM-5a-Pact1-ITE-Tadh1
Plasmid#127723PurposeYeast pathway position 1. ITE transcription unit with the ACT1 promoter and ADH1 terminator.DepositorInsertitaconate transporter from A. terreus
UseSynthetic BiologyExpressionYeastPromoterPact1AvailabilityAcademic Institutions and Nonprofits only -
pDSM-5a-Ppre3-ITE-Teno1
Plasmid#127724PurposeYeast pathway position 1. ITE transcription unit with the PRE3 promoter and ENO1 terminator.DepositorInsertitaconate transporter from A. terreus
UseSynthetic BiologyExpressionYeastPromoterPpre3AvailabilityAcademic Institutions and Nonprofits only -
pDSM-5a-Pact1-ITE-Tdpp1
Plasmid#127725PurposeYeast pathway position 1. ITE transcription unit with the ACT1 promoter and DPP1 terminator.DepositorInsertitaconate transporter from A. terreus
UseSynthetic BiologyExpressionYeastPromoterPact1AvailabilityAcademic Institutions and Nonprofits only -
pDSM-5a-Phta1-ITE-Tvma2
Plasmid#127726PurposeYeast pathway position 1. ITE transcription unit with the HTA1 promoter and VMA2 terminator.DepositorInsertitaconate transporter from A. terreus
UseSynthetic BiologyExpressionYeastPromoterPhta1AvailabilityAcademic Institutions and Nonprofits only -
pDSM-bc-Prps3-CAD-Tcyc1
Plasmid#127728PurposeYeast pathway position 3. CAD transcription unit with the RPS3 promoter and CYC1 terminator.DepositorInsertcis-aconitate decarboxylase
UseSynthetic BiologyExpressionYeastPromoterPrps3AvailabilityAcademic Institutions and Nonprofits only -
pDSM-bc-Peft2-CAD-Tklura3
Plasmid#127729PurposeYeast pathway position 3. CAD transcription unit with the EFT2 promoter and K. lactis URA3 terminator.DepositorInsertcis-aconitate decarboxylase
UseSynthetic BiologyExpressionYeastPromoterPeft2AvailabilityAcademic Institutions and Nonprofits only -
pDSM-bc-Psmtef1-CAD-Tyhi9
Plasmid#127730PurposeYeast pathway position 3. CAD transcription unit with the S. mikatae TEF1 promoter and YHI9 terminator.DepositorInsertcis-aconitate decarboxylase
UseSynthetic BiologyExpressionYeastPromoterPsmtef1AvailabilityAcademic Institutions and Nonprofits only -
pDSM-cd-Pfba1p-ACO-Tgpm1
Plasmid#127731PurposeYeast pathway position 4. ACO transcription unit with the FBA1 promoter and GPM1 terminator.DepositorInsertaconitase
UseSynthetic BiologyExpressionYeastPromoterPfba1AvailabilityAcademic Institutions and Nonprofits only -
pDSM-cd-Pykt6-ACO-Tprm9
Plasmid#127732PurposeYeast pathway position 4. ACO transcription unit with the YKT6 promoter and PRM9 terminator.DepositorInsertaconitase
UseSynthetic BiologyExpressionYeastPromoterPykt6AvailabilityAcademic Institutions and Nonprofits only -
pDSM-cd-Ptef1-ACO-Tyol036w
Plasmid#127733PurposeYeast pathway position 4. ACO transcription unit with the TEF1 promoter and YOL036w terminator.DepositorInsertaconitase
UseSynthetic BiologyExpressionYeastPromoterPtef1AvailabilityAcademic Institutions and Nonprofits only