9,703 results

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mVenus-ATG3-C-18

Plasmid

#56384

Purpose

Localization: Membrane Receptor, Excitation: 515, Emission: 528

Depositor

Michael Davidson

Insert

ATG3 (ATG3 Human)

Tags

mVenus

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 19, 2014

Availability

Industry, Academic Institutions, and Nonprofits
pDONR221_SLC35A1

Plasmid

#132064

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC35A1 (SLC35A1 Human)

Expression

Mammalian

Available Since

Nov. 8, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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RCAN1_pCSdest

Plasmid

#53883

Depositor

Roger Reeves

Article

Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

Insert

RCAN1 (RCAN1 Human)

Expression

Mammalian

Promoter

SP6 and CMV

Available Since

March 27, 2018

Availability

Industry, Academic Institutions, and Nonprofits

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DTX3L

Plasmid

#28266

Depositor

Cheryl Arrowsmith

Insert

DTX3L (DTX3L Human)

Tags

His tag with thrombin cleavage

Expression

Bacterial

Mutation

contains only carboxyl terminal domain (residues …

Available Since

April 14, 2011

Availability

Industry, Academic Institutions, and Nonprofits

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5W9S (CXXC5)

Plasmid

#101266

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Cheryl Arrowsmith

Insert

CXXC5 (CXXC5 Human)

Tags

6xHis-TEV

Expression

Bacterial

Promoter

T7

Available Since

April 3, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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tdEos-MyosinIIB-C-18

Plasmid

#57646

Purpose

Localization: Cytoskeleton, Excitation: 505 / 569, Emission: 516 / 581

Depositor

Michael Davidson

Insert

MyosinIIB (MYH10 Human)

Tags

tdEos

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 13, 2015

Availability

Industry, Academic Institutions, and Nonprofits
pDEST-FRT/T0-GFP-BRCA1 1364-1437

Plasmid

#71119

Purpose

Mammalian expression of GFP-BRCA1 1364-1437

Depositor

Daniel Durocher

Article

Orthwein et al Nature. 2015 Dec 9. doi: 10.1038/nature1

Insert

BRCA1 (BRCA1 Human)

Tags

GFP

Expression

Mammalian

Mutation

contains amino acids 1364-1437 of BRCA1

Promoter

CMV/TO

Available Since

Dec. 9, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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mCrimson3-Tubulin-C-18

Plasmid

#56197

Purpose

Localization: Microtubules, Excitation: 588, Emission: 615

Depositor

Michael Davidson

Insert

Tubulin (TUBA1B Human)

Tags

mCrimson3

Expression

Mammalian

Promoter

CMV

Available Since

March 12, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pGBW-m4133398

Plasmid

#152940

Purpose

Mammalian expression plasmid for SARS-CoV-2 nsp2

Depositor

Ginkgo Bioworks, Benjie Chen

Insert

SARS-CoV-2 nsp2 (ORF1ab Severe acute respiratory syndrome coronavirus 2)

Tags

Cleavable TEV;HA

Expression

Mammalian

Mutation

wt

Promoter

CMV

Available Since

July 30, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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mEos2-TOMM20-N-10

Plasmid

#57429

Purpose

Localization: Mitochondrial Membrane, Excitation: 505 / 569, Emission: 516 / 581

Depositor

Michael Davidson

Insert

TOMM20 (TOMM20 Human)

Tags

mEos2

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 5, 2015

Availability

Industry, Academic Institutions, and Nonprofits
pGBW-m4134407

Plasmid

#152787

Purpose

Mammalian expression plasmid for SARS-CoV-2 nsp10

Depositor

Ginkgo Bioworks, Benjie Chen

Insert

SARS-CoV-2 nsp10 (ORF1ab Severe acute respiratory syndrome coronavirus 2)

Tags

Cleavable TEV;HA

Expression

Mammalian

Mutation

wt

Promoter

CMV

Available Since

July 30, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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mTurquoise-ER-5

Plasmid

#55550

Purpose

Localization: Endoplasmic Reticulum, Excitation: 434, Emission: 474

Depositor

Michael Davidson

Insert

ER (CALR Human)

Tags

mTurquoise

Expression

Mammalian

Mutation

ER Signal Peptide

Promoter

CMV

Available Since

Oct. 2, 2014

Availability

Industry, Academic Institutions, and Nonprofits
Dronpa-Vimentin-7

Plasmid

#57305

Purpose

Localization: Intermediate Filaments, Excitation: 503, Emission: 518

Depositor

Michael Davidson

Insert

Vimentin (VIM Human)

Tags

Dronpa

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 13, 2015

Availability

Industry, Academic Institutions, and Nonprofits
RAB4B

Plasmid

#25514

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Cheryl Arrowsmith

Insert

RAB4B (RAB4B Human)

Tags

His

Expression

Bacterial

Available Since

Aug. 30, 2010

Availability

Industry, Academic Institutions, and Nonprofits
pGBW-m4133478

Plasmid

#152804

Purpose

Mammalian expression plasmid for SARS-CoV-2 nsp4

Depositor

Ginkgo Bioworks, Benjie Chen

Insert

SARS-CoV-2 nsp4 (ORF1ab Severe acute respiratory syndrome coronavirus 2)

Tags

Cleavable TEV;HA

Expression

Mammalian

Mutation

wt

Promoter

CMV

Available Since

July 30, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pGBW-m4133907

Plasmid

#152492

Purpose

Mammalian expression plasmid for SARS-CoV-2 nsp6

Depositor

Ginkgo Bioworks, Benjie Chen

Insert

SARS-CoV-2 nsp6 (ORF1ab Severe acute respiratory syndrome coronavirus 2)

Tags

Cleavable TEV;HA

Expression

Mammalian

Mutation

wt

Promoter

CMV

Available Since

July 30, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pDONR221 SARS-CoV-2 S-frag1_nostop

Plasmid

#153173

Purpose

Gateway-compatible Entry vector, with a fragmented insert of S gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1

Depositor

Fritz Roth, Mikko Taipale

Article

Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

Insert

S-frag1

Use

Gateway-compatible entry vector

Mutation

Many synonymous changes due to codon optimization

Available Since

May 27, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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3BFN

Plasmid

#60111

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Cheryl Arrowsmith

Insert

KIF22:KIF22_dml-X01:C200692 (KIF22 Human)

Expression

Bacterial

Mutation

See comments

Promoter

T7

Available Since

Oct. 22, 2014

Availability

Industry, Academic Institutions, and Nonprofits
pGBW-m4046890

Plasmid

#145740

Purpose

Bacterial Expression plasmid for SARS-CoV-2 ORF6 protein

Depositor

Ginkgo Bioworks, Benjie Chen

Insert

SARS-CoV-2 ORF6 protein (ORF6 Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)

Tags

Cleavable TEV;6xHIS

Expression

Bacterial

Mutation

Escherichia coli recode 1

Promoter

Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

Available Since

April 29, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pGBW-m4134342

Plasmid

#152111

Purpose

Mammalian expression plasmid for SARS-CoV-2 E (envelope)

Depositor

Ginkgo Bioworks, Benjie Chen

Insert

SARS-CoV-2 E (envelope) (E Severe acute respiratory syndrome coronavirus 2)

Tags

Cleavable TEV;3xFlag

Expression

Mammalian

Mutation

wt

Promoter

CMV

Available Since

May 20, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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