9,747 results

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MLKG1delKASH

Plasmid

#130842

Purpose

Expression of plant LINC fluorescent fusion protein for cell biology

Depositor

Hank Bass

Publication

Gumber et al J Cell Sci. 2019 Feb 11;132(3). pii: jcs

Insert

MLKG1

Tags

eGFP-FLAG-HA

Expression

Plant

Mutation

Deletion of KASH domain

Promoter

CaMV 35S

Available since

Sept. 28, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pL0_EF-PtBle

Plasmid

#202180

Purpose

Level 0 plasmid containing a phleomycin antibiotic cassette (fcpF promoter) with EF overhangs used to build a level 1 construct.

Depositor

Christopher Dupont

Publication

Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

Insert

ShBle expression cassette

Use

Synthetic Biology

Mutation

None

Available since

Aug. 29, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pL0_EF-H4

Plasmid

#202178

Purpose

Level 0 plasmid containing a terminator sequence from gene 34971 from Phaeodactylum tricornutum with EF overhangs used to build a level 1 construct.

Depositor

Christopher Dupont

Publication

Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

Insert

H4 terminator

Use

Synthetic Biology

Mutation

None

Available since

Aug. 28, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pL0_EF-fcpA

Plasmid

#202164

Purpose

Level 0 plasmid containing a terminator sequence from gene 18049 from Phaeodactylum tricornutum with EF overhangs used to build a level 1 construct.

Depositor

Christopher Dupont

Publication

Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

Insert

fcpA terminator

Use

Synthetic Biology

Mutation

None

Available since

Aug. 28, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pL0_AC-H4

Plasmid

#202122

Purpose

Level 0 plasmid containing the promoter from gene 34971 from Phaeodactylum tricornutum with AC overhangs used to build a level 1 construct.

Depositor

Christopher Dupont

Publication

Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

Insert

H4 promoter

Use

Synthetic Biology

Mutation

None

Available since

Aug. 24, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pL0_AC-P49

Plasmid

#202120

Purpose

Level 0 plasmid containing the promoter from gene 49202 from Phaeodactylum tricornutum with AC overhangs used to build a level 1 construct.

Depositor

Christopher Dupont

Publication

Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

Insert

P49 promoter

Use

Synthetic Biology

Mutation

None

Available since

Aug. 24, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pCA_e-2

Plasmid

#202097

Purpose

Receiver cloning vector for even-2 level constructs. Contains lacZ for blue/white screening.

Depositor

Christopher Dupont

Publication

Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

Type

Empty backbone

Use

Synthetic Biology

Mutation

None

Available since

Aug. 23, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pCA_e-1

Plasmid

#202096

Purpose

Receiver cloning vector for even-1 level constructs. Contains lacZ for blue/white screening.

Depositor

Christopher Dupont

Publication

Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

Type

Empty backbone

Use

Synthetic Biology

Mutation

None

Available since

Aug. 23, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pL0R_lacZ

Plasmid

#202095

Purpose

Receiver cloning vector for making level 0 parts. Contains lacZ for blue/white screening.

Depositor

Christopher Dupont

Publication

Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

Type

Empty backbone

Use

Synthetic Biology

Mutation

None

Available since

Aug. 23, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pCA_o-4

Plasmid

#202094

Purpose

Receiver cloning vector for odd-4 level constructs. Contains lacZ for blue/white screening.

Depositor

Christopher Dupont

Publication

Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

Type

Empty backbone

Use

Synthetic Biology

Mutation

None

Available since

Aug. 23, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pCA_o-3

Plasmid

#202093

Purpose

Receiver cloning vector for odd-3 level constructs. Contains lacZ for blue/white screening.

Depositor

Christopher Dupont

Publication

Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

Type

Empty backbone

Use

Synthetic Biology

Mutation

None

Available since

Aug. 23, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pCA_o-2

Plasmid

#202092

Purpose

Receiver cloning vector for odd-2 level constructs. Contains lacZ for blue/white screening.

Depositor

Christopher Dupont

Publication

Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

Type

Empty backbone

Use

Synthetic Biology

Mutation

None

Available since

Aug. 23, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pCA_o-1

Plasmid

#202091

Purpose

Receiver cloning vector for odd-1 level constructs. Contains lacZ for blue/white screening.

Depositor

Christopher Dupont

Publication

Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

Type

Empty backbone

Use

Synthetic Biology

Mutation

None

Available since

Aug. 23, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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huntingtin_V2095-V3138_CbioCflag

Plasmid

#194764

Purpose

Baculovirus transfer vector for expression of huntingtin protein in insect and mammalian cells

Depositor

Cheryl Arrowsmith

Insert

HTT (HTT Human)

Use

Baculovirus expression

Tags

Avi-tag (biotin acceptor peptide) and Flag-tag

Expression

Mammalian

Mutation

none

Promoter

CMV and P10

Available since

July 27, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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huntingtin_T97-M2069_NbioCflag

Plasmid

#194763

Purpose

Baculovirus transfer vector for expression of huntingtin protein in insect and mammalian cells

Depositor

Cheryl Arrowsmith

Insert

HTT (HTT Human)

Use

Baculovirus expression

Tags

Avi-tag (biotin acceptor peptide) and Flag-tag

Expression

Mammalian

Mutation

none

Promoter

CMV and P10

Available since

July 26, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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huntingtin_V2095-V3138_NbioCflag

Plasmid

#194765

Purpose

Baculovirus transfer vector for expression of huntingtin protein in insect and mammalian cells

Depositor

Cheryl Arrowsmith

Insert

HTT (HTT Human)

Use

Baculovirus expression

Tags

Avi-tag (biotin acceptor peptide) and Flag-tag

Expression

Mammalian

Mutation

none

Promoter

CMV and P10

Available since

July 26, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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BASIC_SEVA_66.11

Plasmid

#194213

Purpose

BASIC DNA assembly vector

Depositor

Paul Freemont

Publication

Haines et al Synth Biol (Oxf). 2022 Oct 11;7(1):ysac0

Insert

mScarlet counter-selection cassette

Use

Synthetic Biology

Expression

Bacterial

Available since

July 26, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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PATL2_G288-Y543_BV_his

Plasmid

#194760

Depositor

Cheryl Arrowsmith

Insert

PATL2 (PATL2 Human)

Use

Baculovirus expression

Promoter

Polyhedrin

Available since

July 19, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pDONR223 HCoV-OC43 NSP5_nostop

Plasmid

#168690

Purpose

Gateway-compatible Entry vector

Depositor

Fritz Roth

Publication

Weller et al G3 (Bethesda). 2023 Jul 5;13(7):jkad105.

Insert

NSP5 (ORF1ab )

Use

Other

Available since

July 5, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pDONR223 HCoV-OC43 NSP12_nostop

Plasmid

#168696

Purpose

Gateway-compatible Entry vector

Depositor

Fritz Roth

Publication

Weller et al G3 (Bethesda). 2023 Jul 5;13(7):jkad105.

Insert

NSP12 (ORF1ab )

Use

Other

Available since

July 5, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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