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We narrowed to 819 results for: Anti-CRISPR

Showing: 181 - 200 of 819 results

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pUC19-U6-BsmBI_cassette-CjeCas9-sgRNA (KAC482)

Plasmid

#133793

Purpose

U6 promoter sgRNA entry vector used for all CjeCas9 sgRNAs (clone spacer oligos into BsmBI cassette) - similar to V2-TGAA linker sgRNA architecture from Kim et al. Nature Communications 2017

Depositor

Benjamin Kleinstiver, Joseph Bondy-Denomy

Article

Mahendra et al Nat Microbiol. 2020 Apr;5(4):620-629. do

Insert

CjeCas9 sgRNA entry vector

Use

Tags

Expression

Mammalian

Mutation

V2-TGAA linker sgRNA architecture from Kim et al.…

Promoter

U6

Available since

March 30, 2020

Availability

Academic Institutions and Nonprofits only

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pUC19-U6-BsmBI_cassette-St1Cas9-sgRNA (KAC14)

Plasmid

#133791

Purpose

U6 promoter sgRNA entry vector used for all St1Cas9 sgRNAs (clone spacer oligos into BsmBI cassette) - similar to V1 sgRNA architecture from Carter et al. biorxiv 2018

Depositor

Benjamin Kleinstiver, Joseph Bondy-Denomy

Article

Mahendra et al Nat Microbiol. 2020 Apr;5(4):620-629. do

Insert

St1Cas9 sgRNA entry vector

Use

Tags

Expression

Mammalian

Mutation

V1 sgRNA architecture from Carter et al. biorxiv …

Promoter

U6

Available since

March 30, 2020

Availability

Academic Institutions and Nonprofits only

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pUC19-U6-BsmBI_cassette-St3Cas9-sgRNA (KAC27)

Plasmid

#133792

Purpose

U6 promoter sgRNA entry vector used for all St3Cas9 sgRNAs (clone spacer oligos into BsmBI cassette) - similar to sgRNA architecture from Muller et al. Molecular Therapy 2015

Depositor

Benjamin Kleinstiver, Joseph Bondy-Denomy

Article

Mahendra et al Nat Microbiol. 2020 Apr;5(4):620-629. do

Insert

St3Cas9 sgRNA entry vector

Use

Tags

Expression

Mammalian

Mutation

sgRNA architecture from Muller et al. Molecular T…

Promoter

U6

Available since

March 30, 2020

Availability

Academic Institutions and Nonprofits only

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pCS2-AcrIIA4

Plasmid

#174034

Purpose

In vitro transcription of anti-CRISPR AcrIIA4 mRNA

Depositor

James Gagnon

Article

Takasugi et al Genetics. 2021 Nov 4. pii: 6420709. doi:

Insert

AcrIIA4

Use

In vitro transcription

Tags

Expression

Mutation

Promoter

Available since

Feb. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pCS2-AcrIIA2

Plasmid

#174033

Purpose

In vitro transcription of anti-CRISPR AcrIIA2 mRNA

Depositor

James Gagnon

Article

Takasugi et al Genetics. 2021 Nov 4. pii: 6420709. doi:

Insert

AcrIIA2

Use

In vitro transcription

Tags

Expression

Mutation

Promoter

Available since

Feb. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pCS2-AcrIIC3

Plasmid

#174036

Purpose

In vitro transcription of anti-CRISPR AcrIIC3 mRNA

Depositor

James Gagnon

Article

Takasugi et al Genetics. 2021 Nov 4. pii: 6420709. doi:

Insert

AcrIIC3

Use

In vitro transcription

Tags

Expression

Mutation

Promoter

Available since

Nov. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pCS2-AcrIIC1

Plasmid

#174035

Purpose

In vitro transcription of anti-CRISPR AcrIIC1 mRNA

Depositor

James Gagnon

Article

Takasugi et al Genetics. 2021 Nov 4. pii: 6420709. doi:

Insert

AcrIIC1

Use

In vitro transcription

Tags

Expression

Mutation

Promoter

Available since

Nov. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV-T7-AcrIIC3-NLS(SV40) (KAC209)

Plasmid

#134341

Purpose

CMV and T7 promoter expression plasmid for human codon optimized AcrIIC3 with C-terminal NLS (SV40)

Depositor

Benjamin Kleinstiver, Joseph Bondy-Denomy

Article

Mahendra et al Nat Microbiol. 2020 Apr;5(4):620-629. do

Insert

human codon optimized AcrIIC3

Use

Tags

NLS(SV40)

Expression

Mammalian

Mutation

Promoter

CMV and T7

Available since

March 30, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV-T7-AcrIIA4-NLS(SV40) (KAC200)

Plasmid

#133801

Purpose

CMV and T7 promoter expression plasmid for human codon optimized AcrIIA4 with C-terminal NLS (SV40)

Depositor

Benjamin Kleinstiver, Joseph Bondy-Denomy

Article

Mahendra et al Nat Microbiol. 2020 Apr;5(4):620-629. do

Insert

human codon optimized AcrIIA4

Use

Tags

NLS(SV40)

Expression

Mammalian

Mutation

n/a

Promoter

CMV and T7

Available since

March 30, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-T7-AcrIIA5-NLS(SV40) (KAC203)

Plasmid

#133802

Purpose

CMV and T7 promoter expression plasmid for human codon optimized AcrIIA5 with C-terminal NLS (SV40)

Depositor

Benjamin Kleinstiver, Joseph Bondy-Denomy

Article

Mahendra et al Nat Microbiol. 2020 Apr;5(4):620-629. do

Insert

human codon optimized AcrIIA5

Use

Tags

NLS(SV40)

Expression

Mammalian

Mutation

n/a

Promoter

CMV and T7

Available since

March 30, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV-T7-AcrIIA16_Efa-NLS(SV40) (KAC459)

Plasmid

#134337

Purpose

CMV and T7 promoter expression plasmid for human codon optimized AcrIIA16_Efa with C-terminal NLS (SV40)

Depositor

Benjamin Kleinstiver, Joseph Bondy-Denomy

Article

Mahendra et al Nat Microbiol. 2020 Apr;5(4):620-629. do

Insert

human codon optimized AcrIIA16_Efa

Use

Tags

NLS(SV40)

Expression

Mammalian

Mutation

Promoter

CMV and T7

Available since

March 30, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV-T7-AcrIIA16_Lmo-NLS(SV40) (KAC508)

Plasmid

#134332

Purpose

CMV and T7 promoter expression plasmid for human codon optimized AcrIIA16_Lmo with C-terminal NLS (SV40)

Depositor

Benjamin Kleinstiver, Joseph Bondy-Denomy

Article

Mahendra et al Nat Microbiol. 2020 Apr;5(4):620-629. do

Insert

human codon optimized AcrIIA16_Lmo

Use

Tags

NLS(SV40)

Expression

Mammalian

Mutation

Promoter

CMV and T7

Available since

March 30, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-T7-AcrIIC1-NLS(SV40) (KAC208)

Plasmid

#134340

Purpose

CMV and T7 promoter expression plasmid for human codon optimized AcrIIC1 with C-terminal NLS (SV40)

Depositor

Benjamin Kleinstiver, Joseph Bondy-Denomy

Article

Mahendra et al Nat Microbiol. 2020 Apr;5(4):620-629. do

Insert

human codon optimized AcrIIC1

Use

Tags

NLS(SV40)

Expression

Mammalian

Mutation

Promoter

CMV and T7

Available since

March 30, 2020

Availability

Academic Institutions and Nonprofits only

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AAVS1-Puro CAG J591-scFV T-CAR

Plasmid

#171961

Purpose

Donor plasmid for J591 T-CAR expression in human cells

Depositor

Xiaoping Bao

Article

Harris et al J Immunol Regen Med. 2023 May;20:100074.

Insert

Synthetic J591-scFV CAR

Use

CRISPR, Synthetic Biology, and TALEN

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

May 5, 2023

Availability

Academic Institutions and Nonprofits only

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pVAX_CBI_AcrIIA4

Plasmid

#136662

Purpose

Expresses human codon-optimized AcrIIA4 in mammalian cells. ORF flanked by NotI and XhoI restriction sites

Depositor

Yannick Doyon

Article

Hynes et al Nat Commun. 2018 Jul 25;9(1):2919. doi:

Insert

AcrIIA4

Use

Synthetic Biology

Tags

No tag, No NLS

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 28, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pVAX_CBI_AcrIIA2

Plasmid

#136661

Purpose

Expresses human codon-optimized AcrIIA2 in mammalian cells. ORF flanked by NotI and XhoI restriction sites

Depositor

Yannick Doyon

Article

Hynes et al Nat Commun. 2018 Jul 25;9(1):2919. doi:

Insert

AcrIIA2

Use

Synthetic Biology

Tags

No tag, No NLS

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-T7-AcrIIA17_Efa-NLS(SV40) (KAC91)

Plasmid

#134333

Purpose

CMV and T7 promoter expression plasmid for human codon optimized AcrIIA17_Efa with C-terminal NLS (SV40)

Depositor

Benjamin Kleinstiver, Joseph Bondy-Denomy

Article

Mahendra et al Nat Microbiol. 2020 Apr;5(4):620-629. do

Insert

human codon optimized AcrIIA17_Efa

Use

Tags

NLS(SV40)

Expression

Mammalian

Mutation

Promoter

CMV and T7

Available since

March 30, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-T7-AcrIIA17_Sga-NLS(SV40) (KAC92)

Plasmid

#134334

Purpose

CMV and T7 promoter expression plasmid for human codon optimized AcrIIA17_Sga with C-terminal NLS (SV40)

Depositor

Benjamin Kleinstiver, Joseph Bondy-Denomy

Article

Mahendra et al Nat Microbiol. 2020 Apr;5(4):620-629. do

Insert

human codon optimized AcrIIA17_Sga

Use

Tags

NLS(SV40)

Expression

Mammalian

Mutation

Promoter

CMV and T7

Available since

March 30, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-T7-AcrIIA18_Sma-NLS(SV40) (KAC95)

Plasmid

#134335

Purpose

CMV and T7 promoter expression plasmid for human codon optimized AcrIIA18_Sma with C-terminal NLS (SV40)

Depositor

Benjamin Kleinstiver, Joseph Bondy-Denomy

Article

Mahendra et al Nat Microbiol. 2020 Apr;5(4):620-629. do

Insert

human codon optimized AcrIIA18_Sma

Use

Tags

NLS(SV40)

Expression

Mammalian

Mutation

Promoter

CMV and T7

Available since

March 30, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-T7-AcrIIA19_Ssim-NLS(SV40) (KAC97)

Plasmid

#134336

Purpose

CMV and T7 promoter expression plasmid for human codon optimized AcrIIA19_Ssim with C-terminal NLS (SV40)

Depositor

Benjamin Kleinstiver, Joseph Bondy-Denomy

Article

Mahendra et al Nat Microbiol. 2020 Apr;5(4):620-629. do

Insert

human codon optimized AcrIIA19_Ssim

Use

Tags

NLS(SV40)

Expression

Mammalian

Mutation

Promoter

CMV and T7

Available since

March 30, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge

Showing: 181 - 200 of 819 results

Results per page:

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