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We narrowed to 3,609 results for: golden gate

Showing: 181 - 200 of 3609 results

  1. pDM068

    Plasmid
    #216811
    Purpose
    Vector for Aspergillus CRISPR-Cas9 genetic engineering with Golden Gate cloning drop-out cassette for protospacer insertion and NAT selectable marker.
    Depositor
    Ken Sawin
    Article
    Modaffari et al Wellcome Open Res. 2024 Oct 17;9:602. do
    Type
    Empty backbone
    Use
    CRISPR; Fungal expression
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Sept. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    216811-plasmid-map-sequence-id-430142

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  2. pDM026

    Plasmid
    #216808
    Purpose
    Vector for Aspergillus CRISPR-Cas9 genetic engineering with Golden Gate cloning drop-out cassette for protospacer insertion and pyrG selectable marker.
    Depositor
    Ken Sawin
    Article
    Modaffari et al Wellcome Open Res. 2024 Oct 17;9:602. do
    Type
    Empty backbone
    Use
    CRISPR; Fungal expression
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Sept. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    216808-plasmid-map-sequence-id-429385

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  3. pZA8

    Plasmid
    #158486
    Purpose
    Entry clone for Golden Gate assembly. Golden gate compatible N. benthamiana ZAR1 L17E/D481V with STOP codon
    Depositor
    Sophien Kamoun
    Article
    Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.
    Insert
    ZAR1
    Use
    Golden gate entry vector
    Tags
    Expression
    Mutation
    L17E, D481V, STOP codon
    Promoter
    Available since
    Sept. 11, 2020
    Availability
    Academic Institutions and Nonprofits only
    158486-plasmid-map-sequence-id-310749

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  5. pNDGG008

    Plasmid
    #231312
    Purpose
    Vector part for BEVA; BEVA2.0 Position 4 sacB sucrose counterselection module, AmpR.
    Depositor
    Barney Geddes
    Article
    Geddes et al Can J Microbiol. 2025 Jan 1;71:1-13. doi
    Insert
    BEVA2.0 Position 4 sacB sucrose counterselection module, AmpR.
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Feb. 27, 2025
    Availability
    Academic Institutions and Nonprofits only
    231312-plasmid-map-sequence-id-462975

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  6. pDM028

    Plasmid
    #216809
    Purpose
    Vector for Aspergillus CRISPR-Cas9 genetic engineering with Golden Gate cloning drop-out cassette for protospacer insertion and hph selectable marker.
    Depositor
    Ken Sawin
    Article
    Modaffari et al Wellcome Open Res. 2024 Oct 17;9:602. do
    Type
    Empty backbone
    Use
    CRISPR; Fungal expression
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Sept. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    216809-plasmid-map-sequence-id-430135

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  7. pDM030

    Plasmid
    #216810
    Purpose
    Vector for Aspergillus CRISPR-Cas9 genetic engineering with Golden Gate cloning drop-out cassette for protospacer insertion and ble selectable marker.
    Depositor
    Ken Sawin
    Article
    Modaffari et al Wellcome Open Res. 2024 Oct 17;9:602. do
    Type
    Empty backbone
    Use
    CRISPR; Fungal expression
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Sept. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    216810-plasmid-map-sequence-id-430137

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  8. pZA7

    Plasmid
    #158485
    Purpose
    Entry clone for Golden Gate assembly. Golden gate compatible N. benthamiana ZAR1 L17E with STOP codon
    Depositor
    Sophien Kamoun
    Article
    Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.
    Insert
    ZAR1
    Use
    Golden gate entry vector
    Tags
    Expression
    Mutation
    L17E, STOP codon
    Promoter
    Available since
    Sept. 11, 2020
    Availability
    Academic Institutions and Nonprofits only
    158485-plasmid-map-sequence-id-310754

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  9. pZA15

    Plasmid
    #158493
    Purpose
    Entry clone for Golden Gate assembly. Golden gate compatible N. benthamiana ZAR1 L17E without STOP codon
    Depositor
    Sophien Kamoun
    Article
    Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.
    Insert
    ZAR1
    Use
    Golden gate entry vector
    Tags
    Expression
    Mutation
    L17E, no STOP codon
    Promoter
    Available since
    Sept. 11, 2020
    Availability
    Academic Institutions and Nonprofits only
    158493-plasmid-map-sequence-id-310753

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  10. pZA6

    Plasmid
    #158484
    Purpose
    Entry clone for Golden Gate assembly. Golden gate compatible N. benthamiana ZAR1 D481V with STOP codon
    Depositor
    Sophien Kamoun
    Article
    Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.
    Insert
    ZAR1
    Use
    Golden gate entry vector
    Tags
    Expression
    Mutation
    D481V, STOP codon
    Promoter
    Available since
    Aug. 27, 2020
    Availability
    Academic Institutions and Nonprofits only
    158484-plasmid-map-sequence-id-309731

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  12. pZA14

    Plasmid
    #158492
    Purpose
    Entry clone for Golden Gate assembly. Golden gate compatible N. benthamiana ZAR1 D481V without STOP codon
    Depositor
    Sophien Kamoun
    Article
    Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.
    Insert
    ZAR1
    Use
    Golden gate entry vector
    Tags
    Expression
    Mutation
    D481V, no STOP codon
    Promoter
    Available since
    Aug. 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    158492-plasmid-map-sequence-id-309703

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  13. PGR-Blue

    Plasmid
    #68374
    Purpose
    Terminator quantification vector utilizing Golden Gate Assembly and amilCP. Positive colonies are green and false positives are blue. Terminators are placed between GFP and RFP - induced by arabinose.
    Depositor
    Nathan Reyna
    Type
    Empty backbone
    Use
    Synthetic Biology; Golden gate assembly
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    pBAD
    Available since
    Oct. 29, 2015
    Availability
    Academic Institutions and Nonprofits only
    68374-plasmid-map-sequence-id-121945

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  14. pDOC-GG

    Plasmid
    #149377
    Purpose
    Chassis plasmid for building mutagenesis cassettes by Golden Gate assembly to form a donor plasmid for recombineering by Gene Doctoring
    Depositor
    Mark Pallen
    Article
    Thomson et al BMC Biotechnol. 2020 Oct 7;20(1):54. doi
    Inserts
    Fragment containing a kanamycin resistance cassette
    Fragment containing pMB1 origin of replication and an I-SceI recognition sequence
    Fragment containing a lacZ⍺ expression cassette flanked by BsaI sites
    Fragment containing an I-SceI recognition sequence
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 18, 2020
    Availability
    Academic Institutions and Nonprofits only
    149377-plasmid-map-sequence-id-308794

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  15. pZA1

    Plasmid
    #158479
    Purpose
    Entry clone for Golden Gate assembly. Golden Gate compatible wild-type A. thaliana ZAR1 with STOP codon
    Depositor
    Sophien Kamoun
    Article
    Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.
    Insert
    ZAR1 (ZAR1 Mustard Weed)
    Use
    Golden gate entry vector
    Tags
    Expression
    Mutation
    STOP codon
    Promoter
    Available since
    Aug. 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    158479-plasmid-map-sequence-id-309695

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  16. pNDGG005

    Plasmid
    #231309
    Purpose
    Vector part for BEVA; BEVA2.0 Position 3 RSF1010 broad host range origin of replication with oriT, CmR.
    Depositor
    Barney Geddes
    Article
    Geddes et al Can J Microbiol. 2025 Jan 1;71:1-13. doi
    Insert
    BEVA2.0 Position 3 RSF1010 broad host range origin of replication with oriT, CmR.
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    May 8, 2025
    Availability
    Academic Institutions and Nonprofits only
    231309-plasmid-map-sequence-id-472146

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  17. pNDGG006

    Plasmid
    #231310
    Purpose
    Vector part for BEVA; BEVA2.0 Position 3 p15A narrow host range origin of replication with oriT, CmR.
    Depositor
    Barney Geddes
    Article
    Geddes et al Can J Microbiol. 2025 Jan 1;71:1-13. doi
    Insert
    BEVA2.0 Position 3 p15A narrow host range origin of replication with oriT, CmR.
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 4, 2025
    Availability
    Academic Institutions and Nonprofits only
    231310-plasmid-map-sequence-id-463781

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  18. pNDGG007

    Plasmid
    #231311
    Purpose
    Vector part for BEVA; BEVA2.0 Position 3 pMB1 narrow broad host range origin of replication with oriT, CmR.
    Depositor
    Barney Geddes
    Article
    Geddes et al Can J Microbiol. 2025 Jan 1;71:1-13. doi
    Insert
    BEVA2.0 Position 3 pMB1 narrow broad host range origin of replication with oriT, CmR.
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 4, 2025
    Availability
    Academic Institutions and Nonprofits only
    231311-plasmid-map-sequence-id-463780

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  20. pNDGG002

    Plasmid
    #231308
    Purpose
    Vector part for BEVA; BEVA2.0 Position 2 Sp antibiotic resistance via aadA, AmpR/SpR
    Depositor
    Barney Geddes
    Article
    Geddes et al Can J Microbiol. 2025 Jan 1;71:1-13. doi
    Insert
    BEVA2.0 Position 2 Sp antibiotic resistance via aadA, AmpR/SpR
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Feb. 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    231308-plasmid-map-sequence-id-462187

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Showing: 181 - 200 of 3609 results