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pWZLblasti-HA-MEK1-MEK5DD

Plasmid

#53177

Purpose

Expresses HA tagged chimeric human MEK1 (nucleotides 1–201)-MEK5 (498–1344 with S311DT315D) from blasticidin resistance retroviral vector

Depositor

Christopher Counter

Article

Brady et al Nature. 2014 Apr 9. doi: 10.1038/nature1

Insert

MEK1 (1-201)-MEK5 (498–1344) fusion (MAP2K1 Human)

Use

Retroviral

Tags

HA

Expression

Mammalian

Mutation

MEK1 (1-201) MEK5(498–1344 with S311D/T315D)

Promoter

Available since

June 4, 2014

Availability

Academic Institutions and Nonprofits only
M3K5_HUMAN_D0

Plasmid

#79710

Purpose

This plasmid encodes the kinase domain of M3K5. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

Depositor

Markus Seeliger, John Chodera, Nicholas Levinson

Article

Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

Insert

M3K5 (MAP3K5 Human)

Use

Tags

His10-TEV

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only
MAP3K6 gRNA (BRDN0001162255)

Plasmid

#76012

Purpose

3rd generation lentiviral gRNA plasmid targeting human MAP3K6

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

MAP3K6 (MAP3K6 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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MAP3K6 gRNA (BRDN0001144737)

Plasmid

#76013

Purpose

3rd generation lentiviral gRNA plasmid targeting human MAP3K6

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

MAP3K6 (MAP3K6 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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MAP3K6 gRNA (BRDN0001146318)

Plasmid

#76014

Purpose

3rd generation lentiviral gRNA plasmid targeting human MAP3K6

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

MAP3K6 (MAP3K6 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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MAP3K6 gRNA (BRDN0001162229)

Plasmid

#76015

Purpose

3rd generation lentiviral gRNA plasmid targeting human MAP3K6

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

MAP3K6 (MAP3K6 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
MAP3K3 gRNA (BRDN0001148097)

Plasmid

#76016

Purpose

3rd generation lentiviral gRNA plasmid targeting human MAP3K3

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

MAP3K3 (MAP3K3 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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MAP2K2 gRNA (BRDN0001147718)

Plasmid

#77601

Purpose

3rd generation lentiviral gRNA plasmid targeting human MAP2K2

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

MAP2K2 (MAP2K2 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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MAP2K2 gRNA (BRDN0001147931)

Plasmid

#77603

Purpose

3rd generation lentiviral gRNA plasmid targeting human MAP2K2

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

MAP2K2 (MAP2K2 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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MAP2K1 gRNA (BRDN0001145794)

Plasmid

#76210

Purpose

3rd generation lentiviral gRNA plasmid targeting human MAP2K1

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

MAP2K1 (MAP2K1 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge