We narrowed to 3,788 results for: golden gate

1. pJB158

   Plasmid

   #202601

   Purpose

   RP4-based mobilizable plasmid for the IPTG inducible expression of inserts that can be introduced using BsaI-based Golden Gate cloning. Vector has two origins of replication.

   Depositor

   Athanasios Typas

   Article

   Bobonis et al Nat Protoc. 2024 May 9. doi: 10.1038/s41

   Type

   Empty backbone

   Expression

   Bacterial

   Available Since

   Oct. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMH220

   Plasmid

   #122836

   Purpose

   dCas9 with GreenGate B-C flanking sequences

   Depositor

   Markus Schmid

   Article

   Lee et al PLoS One. 2019 Sep 26;14(9):e0222778. do

   Insert

   Nuclease-dead SpCas9 (dCas9), codon-optimised for Arabidopsis
   

   Use

   Golden gate compatible cloning vector

   Mutation

   Contains D10A and H840A mutations

   Available Since

   March 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pPOM033_P7_3"His5

   Plasmid

   #216458

   Purpose

   3' homology arm for S. pombe genomic integration. Part type 7 following the YeastToolkit MoClo grammar.

   Depositor

   Tomáš Pluskal

   Article

   Hebra et al ACS Synth Biol. 2023 Nov 22. doi: 10.102

   Insert

   3''his5
   

   Use

   Synthetic Biology

   Expression

   Yeast

   Mutation

   Removal of any BsaI // BsmBI restriction site

   Available Since

   April 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pPOM026_P2_pNMT1

   Plasmid

   #216451

   Purpose

   Regulatable promoter for gene expression in S. pombe. Part type 2 following the YeastToolkit MoClo grammar.

   Depositor

   Tomáš Pluskal

   Article

   Hebra et al ACS Synth Biol. 2023 Nov 22. doi: 10.102

   Insert

   Promoter nmt1
   

   Use

   Synthetic Biology

   Expression

   Yeast

   Mutation

   Removal of any BsaI // BsmBI restriction site

   Available Since

   April 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pPOM019_P2_pRPL2102

   Plasmid

   #216444

   Purpose

   Constitutive promoter for gene expression in S. pombe. Part type 2 following the YeastToolkit MoClo grammar.

   Depositor

   Tomáš Pluskal

   Article

   Hebra et al ACS Synth Biol. 2023 Nov 22. doi: 10.102

   Insert

   Promoter rpl2102
   

   Use

   Synthetic Biology

   Expression

   Yeast

   Mutation

   Removal of any BsaI // BsmBI restriction site

   Available Since

   March 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pPOM015_P2_pADH1

   Plasmid

   #216440

   Purpose

   Constitutive promoter for gene expression in S. pombe. Part type 2 following the YeastToolkit MoClo grammar.

   Depositor

   Tomáš Pluskal

   Article

   Hebra et al ACS Synth Biol. 2023 Nov 22. doi: 10.102

   Insert

   Promoter adh1
   

   Use

   Synthetic Biology

   Expression

   Yeast

   Mutation

   Removal of any BsaI // BsmBI restriction site

   Available Since

   March 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pPOM013_P2_pENO101

   Plasmid

   #216438

   Purpose

   Constitutive promoter for gene expression in S. pombe. Part type 2 following the YeastToolkit MoClo grammar.

   Depositor

   Tomáš Pluskal

   Article

   Hebra et al ACS Synth Biol. 2023 Nov 22. doi: 10.102

   Insert

   Promoter eno101
   

   Use

   Synthetic Biology

   Expression

   Yeast

   Mutation

   Removal of any BsaI // BsmBI restriction site

   Available Since

   March 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pYPQ148

   Plasmid

   #69300

   Purpose

   Golden Gate recipient and Gateway entry vector; assembly of 8 gRNAs

   Depositor

   Yiping Qi

   Article

   Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

   Type

   Empty backbone

   Use

   CRISPR

   Available Since

   Sept. 14, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCHW00016

   Plasmid

   #222447

   Purpose

   Level 0, C terminal tag module (TTCG-GCTT) containing p65AD-NLS.

   Depositor

   Chih-Hang Wu

   Article

   Chiang et al bioRxiv 2024.03.23.586378

   Insert

   p65AD-NLS
   

   Use

   Synthetic Biology

   Expression

   Plant

   Mutation

   no

   Available Since

   Dec. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCBLsgRNA1

    Plasmid

    #199727

    Purpose

    Golden Gate entry vector; 1st gRNA under OsU3 promoter

    Depositor

    Kan Wang

    Article

    Lee et al Plant Physiol. 2023 Apr 18:kiad231. doi:

    Insert

    OsU3-sgRNA1 scaffold
    

    Expression

    Plant

    Available Since

    April 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCBLsgRNA2

    Plasmid

    #199728

    Purpose

    Golden Gate entry vector; 2nd gRNA under OsU3 promoter

    Depositor

    Kan Wang

    Article

    Lee et al Plant Physiol. 2023 Apr 18:kiad231. doi:

    Insert

    OsU3-sgRNA2 scaffold
    

    Expression

    Plant

    Available Since

    April 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGGF012

    Plasmid

    #48849

    Purpose

    Provides a sulfadiazine resistance cassette as GreenGate module.

    Depositor

    Jan Lohmann

    Article

    Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

    Insert

    pMAS::SulfadiazineR::t35S
    

    Use

    Golden gate compatible cloning vector

    Mutation

    internal BsaI recognition sites in SulfR removed …

    Available Since

    Jan. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBEVY-4420-mCherry

    Plasmid

    #218828

    Purpose

    Yeast surface display of an anti-fluorescene scFv and intracellular expression of mCherry

    Depositor

    Yongku Cho

    Article

    Arbaciauskaite et al Protein Eng Des Sel. 2023 Jan 21;36:gzad

    Insert

    4-4-20 scFv
    

    Expression

    Yeast

    Promoter

    GAL1-10

    Available Since

    May 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBEVY-pT231 scFv-GFP

    Plasmid

    #218827

    Purpose

    Yeast surface display of a phospho-tau pT231 scFv and intracellular expression of GFP

    Depositor

    Yongku Cho

    Article

    Arbaciauskaite et al Protein Eng Des Sel. 2023 Jan 21;36:gzad

    Insert

    pT231 tau scFv
    

    Expression

    Yeast

    Promoter

    GAL1-10

    Available Since

    May 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pICSL50005

    Plasmid

    #117536

    Purpose

    Level0 Golden Gate part: c-terminal tag, YFP

    Depositor

    Nicola Patron

    Article

    Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

    Insert

    c-term tag YFP
    

    Use

    Synthetic Biology

    Mutation

    G65S, L68V, A72S, Y203T, L231H

    Available Since

    Nov. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pEPOR0SP0020

    Plasmid

    #117531

    Purpose

    Level0 Golden Gate part: CDS, SaCas9

    Depositor

    Nicola Patron

    Article

    Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

    Insert

    SaCas9 (with stop codon)
    

    Use

    CRISPR

    Available Since

    Nov. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMH183

    Plasmid

    #122856

    Purpose

    MS2-modified guide RNA targeting the FT promoter with GreenGate D-E flanking sequences

    Depositor

    Markus Schmid

    Article

    Lee et al PLoS One. 2019 Sep 26;14(9):e0222778. do

    Insert

    MS2-modified sgRNA targeting the Arabidopsis FT promoter
    

    Use

    Golden gate compatible cloning vector

    Available Since

    June 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pPD151 ZF37i

    Plasmid

    #138778

    Purpose

    Constitutive expression of ZF37i under the CMV promoter.

    Depositor

    Joshua Leonard

    Article

    Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

    Insert

    ZF37i
    

    Use

    Synthetic Biology

    Tags

    3xFlag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. Cas12c-sgRNA_BbsI_CAM

    Plasmid

    #183221

    Purpose

    Cas12c sgRNA containing BbsI restriction recognition sites in spacer sequence for Golden Gate Assembly

    Depositor

    Jennifer Doudna

    Article

    Huang et al Mol Cell. 2022 Jun 2;82(11):2148-2160.e4

    Insert

    Cas12c single guide RNA containing BbsI sites in spacer sequence
    

    Use

    CRISPR

    Expression

    Bacterial

    Available Since

    June 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCBLsgRNA3

    Plasmid

    #199729

    Purpose

    Golden Gate entry vector; 3rd gRNA under TaU3 promoter

    Depositor

    Kan Wang

    Article

    Lee et al Plant Physiol. 2023 Apr 18:kiad231. doi:

    Insert

    TaU3-sgRNA3 scaffold
    

    Expression

    Plant

    Available Since

    April 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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