We narrowed to 3,663 results for: golden gate

1. Tad-CBE2.1

   Plasmid

   #235053

   Purpose

   Expresses Tad-CBE2.1 in mammalian cells.

   Depositor

   Weixin Tang

   Article

   Wu et al Nat Biotechnol. 2025 Jul 7:10.1038/s4158

   Insert

   Tad-CBE2.1-SpCas9 D10A
   

   Use

   CRISPR

   Tags

   2xUGI, SV40NLS and bpNLS

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. Tad-CABE2.5

   Plasmid

   #235054

   Purpose

   Expresses Tad-CABE2.5 in mammalian cells.

   Depositor

   Weixin Tang

   Article

   Wu et al Nat Biotechnol. 2025 Jul 7:10.1038/s4158

   Insert

   Tad-CABE2.5-SpCas9 D10A
   

   Use

   CRISPR

   Tags

   2xUGI, SV40NLS and bpNLS

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. Tad-CBE2.4

   Plasmid

   #235055

   Purpose

   Expresses Tad-CBE2.4 in mammalian cells.

   Depositor

   Weixin Tang

   Article

   Wu et al Nat Biotechnol. 2025 Jul 7:10.1038/s4158

   Insert

   Tad-CBE2.4-SpCas9 D10A
   

   Use

   CRISPR

   Tags

   2xUGI, SV40NLS and bpNLS

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. Tad-CBE3.1

   Plasmid

   #235056

   Purpose

   Expresses Tad-CBE3.1 in mammalian cells.

   Depositor

   Weixin Tang

   Article

   Wu et al Nat Biotechnol. 2025 Jul 7:10.1038/s4158

   Insert

   Tad-CBE3.1-SpCas9 D10A
   

   Use

   CRISPR

   Tags

   2xUGI, SV40NLS and bpNLS

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. Tad-CBE3.2

   Plasmid

   #235057

   Purpose

   Expresses Tad-CBE3.2 in mammalian cells.

   Depositor

   Weixin Tang

   Article

   Wu et al Nat Biotechnol. 2025 Jul 7:10.1038/s4158

   Insert

   Tad-CBE3.2-SpCas9 D10A
   

   Use

   CRISPR

   Tags

   2xUGI, SV40NLS and bpNLS

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. Tad-CBE3.6

   Plasmid

   #235058

   Purpose

   Expresses Tad-CBE3.6 in mammalian cells.

   Depositor

   Weixin Tang

   Article

   Wu et al Nat Biotechnol. 2025 Jul 7:10.1038/s4158

   Insert

   Tad-CBE3.6-SpCas9 D10A
   

   Use

   CRISPR

   Tags

   2xUGI, SV40NLS and bpNLS

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. Tad-CABE1.1

   Plasmid

   #235051

   Purpose

   Expresses Tad-CABE1.1 in mammalian cells.

   Depositor

   Weixin Tang

   Article

   Wu et al Nat Biotechnol. 2025 Jul 7:10.1038/s4158

   Insert

   Tad-CABE1.1-SpCas9 D10A
   

   Use

   CRISPR

   Tags

   2xUGI, SV40NLS and bpNLS

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. Tad-CABE1.2

   Plasmid

   #235052

   Purpose

   Expresses Tad-CABE1.2 in mammalian cells.

   Depositor

   Weixin Tang

   Article

   Wu et al Nat Biotechnol. 2025 Jul 7:10.1038/s4158

   Insert

   Tad-CABE1.2-SpCas9 D10A
   

   Use

   CRISPR

   Tags

   2xUGI, SV40NLS and bpNLS

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pTE5425

   Plasmid

   #229032

   Purpose

   Expression vector for 10xHis-phi29 DNA polymerase (catalytically inactive mutant D249E): PT7-lacO-g10leader-p2 dead-T7 terminator, CamR

   Depositor

   Tobias Erb

   Article

   Barthel et al bioRxiv 2025.07.08.663768; doi: 10.1101/

   Insert

   PT7-lacO-g10lead-10xHisTag-phi29 DNAP (D249E, catalytically inactive)
   

   Use

   Tags

   10x His-tag + Factor Xa recognition site

   Expression

   Bacterial

   Mutation

   Promoter

   PT7-lacO and T7 promoter

   Available since

   July 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pSWITCH

    Plasmid

    #222402

    Purpose

    Expression in Streptococcus pyogenes

    Depositor

    Anne Botteaux

    Article

    Schiavolin et al Microbiol Spectr. 2024 Oct 3;12(10):e011

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 2, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

11. Hv_HPT:pCSRM2C:Gus_introns

    Plasmid

    #215191

    Purpose

    Evaluating guard cell specific promotors in Barley

    Depositor

    Penny Hundleby

    Article

    Nguyen et al Plant Direct. 2025 Feb 12;9(2):e70045. d

    Insert

    Scream2
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    BdSCRM2

    Available since

    May 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. Bo_NPTII:pCSRM2C:Gus_introns

    Plasmid

    #215177

    Purpose

    Evaluating guard cell specific promotors in Brassica

    Depositor

    Penny Hundleby

    Article

    Nguyen et al Plant Direct. 2025 Feb 12;9(2):e70045. d

    Insert

    Scream2
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    pSCRM2

    Available since

    May 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pRO288

    Plasmid

    #233827

    Purpose

    MoClo-YTK; type4A; Activation domains; VP48

    Depositor

    Jeff Hasty

    Article

    O'Laughlin et al ACS Synth Biol. 2024 Jan 19;13(1):85-102

    Insert

    VP48
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 22, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pRO129

    Plasmid

    #233805

    Purpose

    MoClo-YTK; type3; rtTA systems; rtTA3

    Depositor

    Jeff Hasty

    Article

    O'Laughlin et al ACS Synth Biol. 2024 Jan 19;13(1):85-102

    Insert

    rtTA3
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 22, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pRO232

    Plasmid

    #233808

    Purpose

    MoClo-YTK; type2A; ABA systems; phlfO7

    Depositor

    Jeff Hasty

    Article

    O'Laughlin et al ACS Synth Biol. 2024 Jan 19;13(1):85-102

    Insert

    phlfO7
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 22, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pRO175

    Plasmid

    #233810

    Purpose

    MoClo-YTK; type3B; ABA systems; ABI

    Depositor

    Jeff Hasty

    Article

    O'Laughlin et al ACS Synth Biol. 2024 Jan 19;13(1):85-102

    Insert

    ABI
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 22, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pRO177

    Plasmid

    #233811

    Purpose

    MoClo-YTK; type3B; ABA systems; PYL1

    Depositor

    Jeff Hasty

    Article

    O'Laughlin et al ACS Synth Biol. 2024 Jan 19;13(1):85-102

    Insert

    PYL1
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 22, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pRO286

    Plasmid

    #233812

    Purpose

    MoClo-YTK; type2A; Auxin systems; amtRO7

    Depositor

    Jeff Hasty

    Article

    O'Laughlin et al ACS Synth Biol. 2024 Jan 19;13(1):85-102

    Insert

    amtRO7
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 22, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pWN022

    Plasmid

    #233814

    Purpose

    MoClo-YTK; type3B; Auxin systems; AIDΔ34

    Depositor

    Jeff Hasty

    Article

    O'Laughlin et al ACS Synth Biol. 2024 Jan 19;13(1):85-102

    Insert

    AIDΔ34
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 22, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pWN079

    Plasmid

    #233819

    Purpose

    MoClo-YTK; type3B; NS3 systems; NS3a

    Depositor

    Jeff Hasty

    Article

    O'Laughlin et al ACS Synth Biol. 2024 Jan 19;13(1):85-102

    Insert

    NS3a
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 22, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge