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pLenti-gRNA13-3

Plasmid

#248540

Purpose

A CRISPR-based technology to study chromosome-specific aneuploidy by targeting human centromeres

Depositor

Teresa Davoli

Publication

Bosco et al Cell. 2023 Apr 27;186(9):1985-2001.e19.

Insert

gRNA13-3

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available since

Dec. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pLenti-gRNA13-5

Plasmid

#248541

Purpose

A CRISPR-based technology to study chromosome-specific aneuploidy by targeting human centromeres

Depositor

Teresa Davoli

Publication

Bosco et al Cell. 2023 Apr 27;186(9):1985-2001.e19.

Insert

gRNA13-5

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available since

Nov. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pLenti-gRNA16-1

Plasmid

#248544

Purpose

A CRISPR-based technology to study chromosome-specific aneuploidy by targeting human centromeres

Depositor

Teresa Davoli

Publication

Bosco et al Cell. 2023 Apr 27;186(9):1985-2001.e19.

Insert

gRNA16-1

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available since

Nov. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pLenti-gRNA17-1

Plasmid

#248545

Purpose

A CRISPR-based technology to study chromosome-specific aneuploidy by targeting human centromeres

Depositor

Teresa Davoli

Publication

Bosco et al Cell. 2023 Apr 27;186(9):1985-2001.e19.

Insert

gRNA17-1

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available since

Nov. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pLenti-gRNA21-1

Plasmid

#248549

Purpose

A CRISPR-based technology to study chromosome-specific aneuploidy by targeting human centromeres

Depositor

Teresa Davoli

Publication

Bosco et al Cell. 2023 Apr 27;186(9):1985-2001.e19.

Insert

gRNA21-1

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available since

Nov. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pLenti-gRNA10-3

Plasmid

#248536

Purpose

A CRISPR-based technology to study chromosome-specific aneuploidy by targeting human centromeres

Depositor

Teresa Davoli

Publication

Bosco et al Cell. 2023 Apr 27;186(9):1985-2001.e19.

Insert

gRNA10-3

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available since

Nov. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pLenti-gRNA12-2

Plasmid

#248539

Purpose

A CRISPR-based technology to study chromosome-specific aneuploidy by targeting human centromeres

Depositor

Teresa Davoli

Publication

Bosco et al Cell. 2023 Apr 27;186(9):1985-2001.e19.

Insert

gRNA12-2

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available since

Nov. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pCut_GFP

Plasmid

#240154

Purpose

Expresses CRISPR guides

Depositor

Morten Norholm

Publication

Gude et al bioRxiv 2025.06.19.660532

Inserts

constant cut sites CRISPR guide array
"self-curing" CRISPR guide array
GFP

Use

CRISPR

Expression

Bacterial

Available since

Oct. 22, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-2xcHS4-cjSST-E2-CMVmini-3xHA-mScarlet3-miR-WPRE-hGHpA

Plasmid

#240048

Purpose

Expression of RFP in cortical SST interneurons in marmoset

Depositor

Guoping Feng

Insert

mScarlet3

Use

AAV

Promoter

CMVmini

Available since

Aug. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pMZ2007

Plasmid

#204399

Purpose

A 3OC6-HSL sensor for B. subtilis on a shuttle vector for B. subtilis and E. coli.

Depositor

Lauren Andrews

Publication

Zeng et al ACS Synth Biol. 2023 Dec 11. doi: 10.102

Inserts

quorum sensing regulator LuxR
GFP

Use

Synthetic Biology

Promoter

PftsH and Synthetic promoter PLux0002

Available since

Dec. 12, 2023

Availability

Academic Institutions and Nonprofits only

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pBM23-pyrF-NT

Plasmid

#174384

Purpose

pBM23 contained pyrF and mini-CRISPR.

Depositor

Jing Han

Publication

Lin et al Commun Biol. 2021 Aug 25;4(1):1007. doi:

Insert

pyrF-NT

Use

Template for cloning fragments for chromosomal in…

Expression

Bacterial

Available since

Nov. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pBM23-pyrF-CS

Plasmid

#174396

Purpose

pBM23 contained pyrF and mini-CRISPR.

Depositor

Jing Han

Publication

Lin et al Commun Biol. 2021 Aug 25;4(1):1007. doi:

Insert

pyrF-CS

Use

Template for cloning fragments for chromosomal in…

Expression

Bacterial

Available since

Nov. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pRhaQCas-BsaI

Plasmid

#170631

Purpose

Expression of CRISPR-associated transposases

Depositor

Sheng Yang

Publication

Zhang et al CRISPR J. 2021 Jun;4(3):350-359. doi: 10

Insert

VchTniQ, VchCas8, VchCas7, VchCas6, CRISPR(BsaI)

Expression

Bacterial

Available since

Nov. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pSDH51

Plasmid

#139045

Purpose

MosSCI SapTrap assembly: Cassette 3A for 5-cassette system

Depositor

Erik Griffin

Publication

Fan et al G3 (Bethesda). 2019 Dec 17. pii: g3.119.

Insert

halotag (no STOP codon, PATC introns)

Expression

Worm

Available since

April 16, 2020

Availability

Academic Institutions and Nonprofits only

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pJF7

Plasmid

#139036

Purpose

MosSCI SapTrap assembly: Cassette 2 for 4- or 5-cassette system

Depositor

Erik Griffin

Publication

Fan et al G3 (Bethesda). 2019 Dec 17. pii: g3.119.

Insert

tomm-20 (no STOP codon) (tomm-20 Nematode)

Expression

Worm

Available since

March 6, 2020

Availability

Academic Institutions and Nonprofits only

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pJUMP47ts-2A(sfGFP)

Plasmid

#126992

Purpose

Level 2 vector. sfGFP reporter. Thermosensitive origin pSC101 (low copy number)

Depositor

Chris French

Publication

Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Mutation

RepA A56V thermosensitive mutation

Available since

Aug. 27, 2019

Availability

Academic Institutions and Nonprofits only

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pJUMP27ts-1A(lacZ)

Plasmid

#126977

Purpose

Level 1 vector. With lacZ as alternative cloning reporter. Thermosensitive origin pSC101 (low copy number)

Depositor

Chris French

Publication

Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Mutation

RepA A56V thermosensitive mutation

Available since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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BSP179

Plasmid

#110915

Purpose

For cloning expression cassettes by HR in yeast, Universal MosSCI compatible

Depositor

Alexander Mendenhall

Publication

Sands et al Transl Med Aging. 2018 Jan;2:1-10. doi:

Type

Empty backbone

Use

Synthetic Biology; Yeast homologous recombination…

Expression

Worm

Available since

June 22, 2018

Availability

Academic Institutions and Nonprofits only

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pICH87644

Plasmid

#50274

Depositor

Sylvestre Marillonnet, Nicola Patron

Publication

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

promoter, act2 (AT3G18780, A. thaliana) and 5'UTR, omega (Tobacco Mosaic Virus)

Use

Plant expression

Mutation

BsaI/BbsI sites removed by point-mutation

Available since

March 7, 2014

Availability

Academic Institutions and Nonprofits only

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pAGM1479

Plasmid

#50291

Depositor

Sylvestre Marillonnet

Publication

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

5'UTR, omega (Tobacco Mosaic Virus) + N terminal HIS tag (6x polyhistidine)

Use

Plant expression

Mutation

BsaI/BbsI sites removed by point-mutation

Available since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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