Addgene

We narrowed to 3,518 results for: Mak

Showing: 3001 - 3030 of 3518 results

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pJC225

Plasmid

#176883

Purpose

MoClo Level1 plasmid, allows transition into Cermak, et al., 2017 GG cloning system

Depositor

Daniel Voytas

Article

Cody et al Nat Protoc. 2022 Oct 17. pii: 10.1038/s4

Type

Empty backbone

Use

Cloning

Tags

Expression

Mutation

Promoter

Available since

May 9, 2022

Availability

Academic Institutions and Nonprofits only

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MSCV-6NBC-GFP-PGK-Puro-Barcode1

Plasmid

#83678

Purpose

individual 6N barcode to make barcoded cell line variants

Depositor

Monte Winslow

Article

Gruner et al Nat Methods. 2016 Sep 12. doi: 10.1038/n

Insert

Barcode1

Use

Retroviral

Tags

Expression

Mutation

Promoter

Available since

Nov. 8, 2016

Availability

Academic Institutions and Nonprofits only

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pMIII-Neo

Plasmid

#153422

Purpose

MSCV with IRES-Neomycin resistant gene

Depositor

Maki Nakayama

Article

Landry et al Bio Protoc. 2021 Jan 20;11(2):e3883. doi

Type

Empty backbone

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 11, 2020

Availability

Academic Institutions and Nonprofits only

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px330-USP13

Plasmid

#61812

Purpose

to make USP13 Knockout cell line

Depositor

Yihong Ye

Article

Liu et al Elife. 2014;3:e01369. doi: 10.7554/eLife

Insert

USP13 (USP13 Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

U6

Available since

Feb. 5, 2015

Availability

Academic Institutions and Nonprofits only
pTol-Promoter:NLS-tdMCP-eGFP

Plasmid

#86248

Purpose

For generating transgenic lines that ubiquitously expressing the fluorescent MCP reporter

Depositor

Florence Marlow

Article

Campbell et al Development. 2015 Apr 1;142(7):1368-74.

Insert

beta Actin promoter:NLS-tdMCPeGFP

Use

Used with transposase to make transient or stable…

Tags

NLS-tdMCP-eGFP

Expression

Mutation

Promoter

Beta Actin

Available since

Jan. 25, 2017

Availability

Academic Institutions and Nonprofits only

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H2B-mGarnet

Plasmid

#104311

Purpose

codes for a fusion of fluorescent protein mGarnet and human histon 2B (H2B), makes the H2B visible in live cells

Depositor

Ulrich Nienhaus

Article

Hense et al Sci Rep. 2015 Dec 9;5:18006. doi: 10.103

Insert

H2B-mGarnet

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 25, 2018

Availability

Academic Institutions and Nonprofits only

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p221z-AtMIR390a

Plasmid

#118388

Purpose

Entry clone containing AtMIR390a backbone (split by ccdB gene). Used to make artificial microRNA construct. For use in plants and compatible with the MultiSite Gateway system.

Depositor

Ari Pekka Mähönen

Article

Wang et al Nat Plants. 2020 Jun 29. pii: 10.1038/s4

Insert

AtMIR390a backbone

Use

RNAi

Tags

Expression

Mutation

Promoter

Available since

Sept. 24, 2019

Availability

Academic Institutions and Nonprofits only

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pERB219

Plasmid

#61501

Purpose

Const. Halotag-GFP-PACT + mCherry-DHFR

Depositor

Michael Lampson, David Chenoweth

Article

Ballister et al Nat Commun. 2014 Nov 17;5:5475. doi: 10.

Inserts

HaloTag-GFP-PACT
mCherry-DHFR

Use

Tags

HaloTag and mCherry

Expression

Mammalian

Mutation

Promoter

Available since

May 18, 2015

Availability

Academic Institutions and Nonprofits only

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pTAPmyc-T2A-tagRFP

Plasmid

#140278

Purpose

Backbone for making a mammalian expression vector of the protein of interest

Depositor

Hirohide Saito

Article

Kawasaki et al Nucleic Acids Res. 2017 May 19. doi: 10.

Insert

Empty backbone

Use

Synthetic Biology

Tags

myc-T2A-tagRFP

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 2, 2020

Availability

Academic Institutions and Nonprofits only

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mCherry-Cb5-IRES-puromycin-pLVx-EF1a

Plasmid

#177434

Purpose

To express mCherry fused to Cb5 endoplasmic reticulum (ER) targeting sequence. Lentiviral vector used to make cell lines expressing this ER landmark.

Depositor

David Andrews

Insert

Cb5 tail anchor (Cyb5r4 Rat)

Use

Lentiviral

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Jan. 3, 2023

Availability

Academic Institutions and Nonprofits only

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TALED_Left-stuffer-8e-E1347A

Plasmid

#187163

Purpose

original vector to make all constructs

Depositor

Jin-Soo Kim

Article

Cho et al Cell. 2022 May 12;185(10):1764-1776.e12.

Insert

Left-stuffer-8e-E1347A

Use

Tags

Expression

Mammalian

Mutation

Promoter

pCMV

Available since

Aug. 9, 2022

Availability

Academic Institutions and Nonprofits only

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TALED_Left-stuffer-1397C-8e

Plasmid

#187169

Purpose

original vector to make all constructs

Depositor

Jin-Soo Kim

Article

Cho et al Cell. 2022 May 12;185(10):1764-1776.e12.

Insert

Left-stuffer-1397C-8e

Use

Tags

Expression

Mammalian

Mutation

Promoter

pCMV

Available since

Aug. 9, 2022

Availability

Academic Institutions and Nonprofits only

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TALED_Left-stuffer-1397C-UGI

Plasmid

#187168

Purpose

original vector to make all constructs

Depositor

Jin-Soo Kim

Article

Cho et al Cell. 2022 May 12;185(10):1764-1776.e12.

Insert

Left-stuffer-1397C-UGI

Use

Tags

Expression

Mammalian

Mutation

Promoter

pCMV

Available since

Aug. 5, 2022

Availability

Academic Institutions and Nonprofits only

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TALED_Right-stuffer-1397N-UGI

Plasmid

#187173

Purpose

original vector to make all constructs

Depositor

Jin-Soo Kim

Article

Cho et al Cell. 2022 May 12;185(10):1764-1776.e12.

Insert

Right-stuffer-1397N-UGI

Use

Tags

Expression

Mammalian

Mutation

Promoter

pCMV

Available since

Aug. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pMSSM97: pCMV-HA-NLS-SpuFz1-E498R

Plasmid

#205275

Purpose

Human expression vector for human codon optimized N-terminal NLS-tagged SpuFz1-E498R

Depositor

Feng Zhang

Article

Saito et al Nature. 2023 Jun 28. doi: 10.1038/s41586

Insert

SpuFz1

Use

Tags

HA, NLS(Makkerh)

Expression

Mammalian

Mutation

E498R

Promoter

Available since

Aug. 15, 2023

Availability

Academic Institutions and Nonprofits only

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pMSSM105: pCMV-HA-NLS-SpuFz1-T513K

Plasmid

#205276

Purpose

Human expression vector for human codon optimized N-terminal NLS-tagged SpuFz1-T513K

Depositor

Feng Zhang

Article

Saito et al Nature. 2023 Jun 28. doi: 10.1038/s41586

Insert

SpuFz1

Use

Tags

HA, NLS(Makkerh)

Expression

Mammalian

Mutation

T513K

Promoter

Available since

Aug. 15, 2023

Availability

Academic Institutions and Nonprofits only

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pMSSM58: pCMV-HA-NLS-SpuFz1-D487K

Plasmid

#205274

Purpose

Human expression vector for human codon optimized N-terminal NLS-tagged SpuFz1-D487K

Depositor

Feng Zhang

Article

Saito et al Nature. 2023 Jun 28. doi: 10.1038/s41586

Insert

SpuFz1

Use

Tags

HA, NLS(Makkerh)

Expression

Mammalian

Mutation

D487K

Promoter

Available since

Aug. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pMSSM59: pCMV-HA-NLS-SpuFz1-D300R

Plasmid

#205272

Purpose

Human expression vector for human codon optimized N-terminal NLS-tagged SpuFz1-D300R

Depositor

Feng Zhang

Article

Saito et al Nature. 2023 Jun 28. doi: 10.1038/s41586

Insert

SpuFz1

Use

Tags

HA, NLS(Makkerh)

Expression

Mammalian

Mutation

D300R

Promoter

Available since

Aug. 9, 2023

Availability

Academic Institutions and Nonprofits only

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KI-eab2-AA

Plasmid

#182157

Purpose

Knockin plasmid with homologous arm for making zMADM-AA zebrafish line

Depositor

Hui Zong

Article

Xu et al Proc Natl Acad Sci U S A. 2022 Mar 1;119

Insert

Homologous arm

Use

Zebrafish expression

Tags

Expression

Mutation

Promoter

eab2

Available since

May 20, 2022

Availability

Academic Institutions and Nonprofits only

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KI-eab2-GG

Plasmid

#182158

Purpose

Knockin plasmid with homologous arm for making zMADM-GG zebrafish line

Depositor

Hui Zong

Article

Xu et al Proc Natl Acad Sci U S A. 2022 Mar 1;119

Insert

Homologous arm

Use

Zebrafish expression

Tags

Expression

Mutation

Promoter

eab2

Available since

May 20, 2022

Availability

Academic Institutions and Nonprofits only

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KI-eab2-AG

Plasmid

#182159

Purpose

Knockin plasmid with homologous arm for making zMADM-AG zebrafish line

Depositor

Hui Zong

Article

Xu et al Proc Natl Acad Sci U S A. 2022 Mar 1;119

Insert

Homologous arm

Use

Zebrafish expression

Tags

Expression

Mutation

Promoter

eab2

Available since

May 20, 2022

Availability

Academic Institutions and Nonprofits only

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KI-eab2-GA

Plasmid

#182160

Purpose

Knockin plasmid with homologous arm for making zMADM-GA zebrafish line

Depositor

Hui Zong

Article

Xu et al Proc Natl Acad Sci U S A. 2022 Mar 1;119

Insert

Homologous arm

Use

Zebrafish expression

Tags

Expression

Mutation

Promoter

eab2

Available since

May 20, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
TALED_Right-stuffer-1397C-8e

Plasmid

#187170

Purpose

original vector to make all constructs

Depositor

Jin-Soo Kim

Article

Cho et al Cell. 2022 May 12;185(10):1764-1776.e12.

Insert

Right-stuffer-1397C-8e

Use

Tags

Expression

Mammalian

Mutation

Promoter

pCMV

Available since

Aug. 9, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
TALED_Left-stuffer-1397N

Plasmid

#187167

Purpose

original vector to make all constructs

Depositor

Jin-Soo Kim

Article

Cho et al Cell. 2022 May 12;185(10):1764-1776.e12.

Insert

Left-stuffer-1397N

Use

Tags

Expression

Mammalian

Mutation

Promoter

pCMV

Available since

Aug. 9, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
TALED_Right-stuffer-8e-E1347A

Plasmid

#187166

Purpose

original vector to make all constructs

Depositor

Jin-Soo Kim

Article

Cho et al Cell. 2022 May 12;185(10):1764-1776.e12.

Insert

Right-stuffer-8e-E1347A

Use

Tags

Expression

Mammalian

Mutation

Promoter

pCMV

Available since

Aug. 9, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
TALED_Right-stuffer-8e

Plasmid

#187165

Purpose

original vector to make all constructs

Depositor

Jin-Soo Kim

Article

Cho et al Cell. 2022 May 12;185(10):1764-1776.e12.

Insert

Right-stuffer-8e

Use

Tags

Expression

Mammalian

Mutation

Promoter

pCMV

Available since

Aug. 9, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
TALED_Right-stuffer-E1347A

Plasmid

#187164

Purpose

original vector to make all constructs

Depositor

Jin-Soo Kim

Article

Cho et al Cell. 2022 May 12;185(10):1764-1776.e12.

Insert

Right-stuffer-E1347A

Use

Tags

Expression

Mammalian

Mutation

Promoter

pCMV

Available since

Aug. 9, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
TALED_Left-stuffer-8e

Plasmid

#187162

Purpose

original vector to make all constructs

Depositor

Jin-Soo Kim

Article

Cho et al Cell. 2022 May 12;185(10):1764-1776.e12.

Insert

stuffer-8e

Use

Tags

Expression

Mammalian

Mutation

Promoter

pCMV

Available since

Aug. 9, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
TALED_Left-stuffer-E1347A

Plasmid

#187161

Purpose

original vector to make all constructs

Depositor

Jin-Soo Kim

Article

Cho et al Cell. 2022 May 12;185(10):1764-1776.e12.

Insert

Left-stuffer-E1347A

Use

Tags

Expression

Mammalian

Mutation

Promoter

pCMV

Available since

Aug. 9, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
TALED_Left-stuffer-1397N-UGI

Plasmid

#187174

Purpose

original vector to make all constructs

Depositor

Jin-Soo Kim

Article

Cho et al Cell. 2022 May 12;185(10):1764-1776.e12.

Insert

Left-stuffer-1397N-UGI

Use

Tags

Expression

Mammalian

Mutation

Promoter

pCMV

Available since

Aug. 5, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge