We narrowed to 12,122 results for: cel.2

1. pNW538_pAAVS1-pAP1(2)-FlpO-ABI-P2A-PYL-FlpO-CAG-U1-frt-STOP-frt-U2-GFP-P2A-luc2-ZeoR

   Plasmid

   #192939

   Purpose

   FlpO-based digitizer circuit driven by AP1 promoter

   Depositor

   Wilson Wong

   Article

   Wong et al Nat Commun. 2021 Oct 22;12(1):6150. doi:

   Inserts

   AP1(2)/minTK promoter
   FlpO-ABI
   PYL-FlpO
   CAG promoter
   frt-STOP-frt
   GFP
   luciferase
   pPGK
   Zeocin resistance
   BFP
   

   Use

   Synthetic Biology

   Expression

   Mammalian

   Available Since

   Dec. 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEGFP/golgin-160/3DE

   Plasmid

   #180631

   Purpose

   Expression of caspase-resistant golgin-160

   Depositor

   Carolyn Machamer

   Article

   Mancini et al J Cell Biol. 2000 May 1;149(3):603-12. d

   Insert

   golgin-160/3DE (GOLGA3 Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   D59E, D139E, D311E

   Available Since

   March 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pHR KIF17 (1-738)

   Plasmid

   #60406

   Purpose

   Expression of truncated form of human homodimeric kinesin-2 (KIF17) amino acids 1-738

   Depositor

   Ron Vale

   Article

   Sirajuddin et al Nat Cell Biol. 2014 Apr;16(4):335-44. do

   Insert

   KIF17 (KIF17 Human)
   

   Use

   Lentiviral

   Tags

   GB1, Strep-Tag, and sfGFP

   Expression

   Mammalian

   Mutation

   Truncated version amino acids 1-738

   Promoter

   SFFV

   Available Since

   Jan. 26, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pIRESneo-FLAG/HA Ago2

   Plasmid

   #10821

   Depositor

   Thomas Tuschl

   Article

   Meister et al Mol Cell. 2004 Jul 23. 15(2):185-97.

   Insert

   Argonaute 2 (AGO2 Human)
   

   Tags

   FLAG and HA

   Expression

   Mammalian

   Mutation

   R668H. Additionally, N terminus different from wi…

   Available Since

   Oct. 14, 2005

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAc5.1B-lambdaN-DmPABPC1-Cterm-V5His6_H

   Plasmid

   #146488

   Purpose

   Insect Expression of DmPABPC1-Cterm

   Depositor

   Elisa Izaurralde

   Article

   Zekri et al Mol Cell Biol. 2009 Dec;29(23):6220-31.

   Insert

   DmPABPC1-Cterm (pAbp Fly)
   

   Expression

   Insect

   Available Since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAc5.1B-lambdaN-DmPABPC1-Nterm-V5His6_H

   Plasmid

   #146489

   Purpose

   Insect Expression of DmPABPC1-Nterm

   Depositor

   Elisa Izaurralde

   Article

   Zekri et al Mol Cell Biol. 2009 Dec;29(23):6220-31.

   Insert

   DmPABPC1-Nterm (pAbp Fly)
   

   Expression

   Insect

   Available Since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAc5.1B-DmPABPC1-Nterm-V5His6_H

   Plasmid

   #146478

   Purpose

   Insect Expression of DmPABPC1-Nterm

   Depositor

   Elisa Izaurralde

   Article

   Zekri et al Mol Cell Biol. 2009 Dec;29(23):6220-31.

   Insert

   DmPABPC1-Nterm (pAbp Fly)
   

   Expression

   Insect

   Available Since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pHIV-EGFP.Flag-EZH2-mt2

   Plasmid

   #220245

   Purpose

   Expresses an EZH2 mutant for knockout/rescue experiments in mammalian cells.

   Depositor

   Chen Davidovich

   Article

   Gail et al Nat Genet. 2024 May 14. doi: 10.1038/s41

   Insert

   EZH2 mt 2 (EZH2 Human)
   

   Use

   Lentiviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   F32A, R34A, D36A, K39A, PRKKKR494-499NAAIRS

   Promoter

   EF-1α

   Available Since

   June 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. GABA(A) receptor subunit a2SE

   Plasmid

   #49169

   Purpose

   pHluorin-tagged GABA A receptor subunit (alpha 2) produces minimal fluorescence during trafficking and a robust fluorescent signal at the cell surface

   Depositor

   Tija Jacob, Stephen Moss

   Article

   Tretter et al J Neurosci. 2008 Feb 6;28(6):1356-65. do

   Insert

   GABA(A) receptor subunit alpha-2 (GABRA2 Human)
   

   Tags

   HA, bovine alpha 1 signal sequence, and pHGFP (pH…

   Expression

   Mammalian

   Mutation

   T343A

   Promoter

   CMV

   Available Since

   Dec. 10, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3-sACE2v2-Fc(IgG1)

    Plasmid

    #145165

    Purpose

    Mammalian expression plasmid for soluble ACE2-Fc fusion (IgG1) high affinity 2

    Depositor

    Erik Procko

    Article

    Chan et al Science. 2020 Sep 4;369(6508):1261-1265.

    Insert

    Angiotensin-converting enzyme 2 (ACE2 Human)
    

    Tags

    Fc region of human IgG1

    Expression

    Mammalian

    Mutation

    Extracellular, soluble protease domain (a.a. M1-D…

    Promoter

    CMV

    Available Since

    April 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pFl Strep-sumo-TEV-hAgo2 D597N

    Plasmid

    #136236

    Purpose

    Expressing catalytically inactive human Argonaute-2 in insect cells

    Depositor

    Leemor Joshua-Tor

    Article

    Elkayam et al Nucleic Acids Res. 2017 Apr 7;45(6):3528

    Insert

    human Argonaute-2 (AGO2 Human)
    

    Tags

    Sterp Sumo

    Expression

    Insect

    Mutation

    D597N

    Promoter

    polyhedrin

    Available Since

    April 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3-sACE2v2-sfGFP

    Plasmid

    #145173

    Purpose

    Mammalian expression plasmid for sACE2-sfGFP high affinity variant 2

    Depositor

    Erik Procko

    Article

    Chan et al Science. 2020 Sep 4;369(6508):1261-1265.

    Insert

    Angiotensin-converting enzyme 2 (ACE2 Human)
    

    Tags

    Linker GSGGSGSGG and superfolder GFP

    Expression

    Mammalian

    Mutation

    Extracellular, soluble protease domain (a.a. M1-D…

    Promoter

    CMV

    Available Since

    April 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3-sACE2v2-8his

    Plasmid

    #149270

    Purpose

    Mammalian expression plasmid for his-tagged sACE2 high affinity variant 2

    Depositor

    Erik Procko

    Article

    Chan et al Science. 2020 Sep 4;369(6508):1261-1265.

    Insert

    Angiotensin-converting enzyme 2 (ACE2 Human)
    

    Tags

    Short GSG linker and 8his tag

    Expression

    Mammalian

    Mutation

    Extracellular, soluble protease domain (a.a. M1-D…

    Promoter

    CMV

    Available Since

    May 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3-sACE2v2

    Plasmid

    #145149

    Purpose

    Mammalian expression plasmid for soluble ACE2 (protease domain) high affinity variant 2

    Depositor

    Erik Procko

    Article

    Chan et al Science. 2020 Sep 4;369(6508):1261-1265.

    Insert

    Angiotensin-converting enzyme 2 (ACE2 Human)
    

    Expression

    Mammalian

    Mutation

    Extracellular, soluble protease domain (a.a. M1-D…

    Promoter

    CMV

    Available Since

    April 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pcDNA3-sACE2v2-Fc(IgA1)

    Plasmid

    #145157

    Purpose

    Mammalian expression plasmid for soluble ACE2-Fc fusion (IgA1) high affinity variant 2

    Depositor

    Erik Procko

    Article

    Chan et al Science. 2020 Sep 4;369(6508):1261-1265.

    Insert

    Angiotensin-converting enzyme 2 (ACE2 Human)
    

    Tags

    Fc region of human IgA1

    Expression

    Mammalian

    Mutation

    Extracellular, soluble protease domain (a.a. M1-D…

    Promoter

    CMV

    Available Since

    April 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pv6_MECP2_MBD-R106W

    Plasmid

    #179408

    Purpose

    Cell line generation via recombination-mediated cassette exchange (RMCE) and stable expression of MECP2_MBD(R106W)

    Depositor

    Tuncay Baubec

    Article

    Villasenor et al Nat Biotechnol. 2020 Mar 2. pii: 10.1038

    Insert

    MECP2_MBD(R106W) (Mecp2 Mouse)
    

    Tags

    AviTag and EGFP

    Expression

    Mammalian

    Mutation

    MBD R106W

    Promoter

    CAGGS

    Available Since

    Aug. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pv6_MECP2_MBD-R133C

    Plasmid

    #179409

    Purpose

    Cell line generation via recombination-mediated cassette exchange (RMCE) and stable expression of MECP2_MBD(R133C)

    Depositor

    Tuncay Baubec

    Article

    Villasenor et al Nat Biotechnol. 2020 Mar 2. pii: 10.1038

    Insert

    MECP2_MBD(R133C) (Mecp2 Mouse)
    

    Tags

    AviTag and EGFP

    Expression

    Mammalian

    Mutation

    MBD R133C

    Promoter

    CAGGS

    Available Since

    Sept. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pv6_MECP2_MBD-T158M

    Plasmid

    #179410

    Purpose

    Cell line generation via recombination-mediated cassette exchange (RMCE) and stable expression of MECP2_MBD(T158M)

    Depositor

    Tuncay Baubec

    Article

    Villasenor et al Nat Biotechnol. 2020 Mar 2. pii: 10.1038

    Insert

    MECP2_MBD(T158M) (Mecp2 Mouse)
    

    Tags

    AviTag and EGFP

    Expression

    Mammalian

    Mutation

    MBD T158M

    Promoter

    CAGGS

    Available Since

    Aug. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCMV6-AN-DDK_3KA + S580A POLI

    Plasmid

    #131241

    Purpose

    Mammalian expression of N-terminally FLAG-tagged DNA polymerase iota containing mutations K440A, K444A, K446A and S580A

    Depositor

    Roger Woodgate

    Article

    Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. d

    Insert

    DNA polymerase iota (POLI Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Contains point mutations that convert Pol iota se…

    Available Since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3-sACE2v2(732)

    Plasmid

    #154099

    Purpose

    Mammalian expression plasmid for dimeric soluble ACE2 high affinity variant 2 (untagged)

    Depositor

    Erik Procko

    Article

    Chan et al Science. 2020 Sep 4;369(6508):1261-1265.

    Insert

    Angiotensin-converting enzyme 2 (ACE2 Human)
    

    Expression

    Mammalian

    Mutation

    Extracellular, soluble protease and neck domains …

    Promoter

    CMV

    Available Since

    June 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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