2,294 results

1. pcDNA-rUSP19ER-siRNAresistant

   Plasmid

   #155251

   Purpose

   for mammalian overexpression of the siRNA resistant ER isoform of rat USP19

   Depositor

   Simon Wing

   Insert

   USP19 (ER isoform) siRNA resistant (Usp19 Rat)
   

   Tags

   6XHis-3XFlag

   Expression

   Mammalian

   Mutation

   Lys(23)AAG to AAA, Lys(24)AAG to AAA, Gln(26)CAG …

   Promoter

   CMV

   Available Since

   Oct. 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pcDNA-rUSP19CYT-siRNAresistant

   Plasmid

   #155252

   Purpose

   for mammalian overexpression of the siRNA resistant CYT isoform of rat USP19

   Depositor

   Simon Wing

   Insert

   USP19 (CYT isoform) siRNA resistant (Usp19 Rat)
   

   Tags

   6XHis-3XFlag

   Expression

   Mammalian

   Mutation

   Lys(23)AAG to AAA, Lys(24)AAG to AAA, Gln(26)CAG …

   Promoter

   CMV

   Available Since

   Oct. 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pcDNA-HisFlag-rUSP19ER-CAmut

   Plasmid

   #155237

   Purpose

   for mammalian overexpression of the mutated ER isoform of rat USP19

   Depositor

   Simon Wing

   Article

   Lu et al Mol Cell Biol. 2009 Jan;29(2):547-58. do

   Insert

   USP19 (ER isoform) (Usp19 Rat)
   

   Tags

   6XHis-3XFlag

   Expression

   Mammalian

   Mutation

   Cys(545) to Ala

   Promoter

   CMV

   Available Since

   Oct. 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pcDNA-HisFlag-rUSP19CSdomains

   Plasmid

   #155240

   Purpose

   for mammalian overexpression of the CS domains of rat USP19

   Depositor

   Simon Wing

   Insert

   USP19 (CS domains) (Usp19 Rat)
   

   Tags

   6XHis-3XFlag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. CBSH3+ shRNA-2

   Plasmid

   #160068

   Purpose

   Knock Down SH3+ Collybistin isoforms

   Depositor

   Angel de Blas

   Article

   George et al J Neurochem. 2021 May;157(4):1032-1051.

   Insert

   ARHGEF9 (Arhgef9 Rat)
   

   Use

   RNAi

   Promoter

   mouse U6 promoter

   Available Since

   Oct. 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. CBSH3+ 3m shRNA-1

   Plasmid

   #160067

   Purpose

   3-point mutation negative control for CBSH3+ shRNA-1 targeting collybistin isoforms with SH3+ domain (plasmid 160066)

   Depositor

   Angel de Blas

   Article

   George et al J Neurochem. 2021 May;157(4):1032-1051.

   Insert

   ARHGEF9 (Arhgef9 Rat)
   

   Use

   RNAi

   Mutation

   GGcTGaTTaCAACAAGGATTG (mutations shown in lower c…

   Promoter

   mouse U6 promoter

   Available Since

   Oct. 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. CBSH3+ shRNA-1

   Plasmid

   #160066

   Purpose

   Knock Down SH3+ Collybistin isoforms

   Depositor

   Angel de Blas

   Article

   George et al J Neurochem. 2021 May;157(4):1032-1051.

   Insert

   ARHGEF9 (Arhgef9 Rat)
   

   Use

   RNAi

   Promoter

   mouse U6 promoter

   Available Since

   Oct. 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. CBSH3- 3m shRNA3

   Plasmid

   #160213

   Purpose

   Knock Down MYDSH3- Collybistin isoforms, 3-point mutation negative control for CBSH3- shRNA3 targeting collybistin MYD-CBSH3- isoforms (Plasmid #160212)

   Depositor

   Angel de Blas

   Article

   George et al J Neurochem. 2021 May;157(4):1032-1051.

   Insert

   ARHGEF9 (Arhgef9 Rat)
   

   Use

   RNAi

   Mutation

   TGTATGACCTCaGGcTGtACCA (mutations shown in lower …

   Promoter

   mouse U6 promoter

   Available Since

   Oct. 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. CBSH3- shRNA2

   Plasmid

   #160210

   Purpose

   Knock Down MTLSH3- Collybistin isoforms

   Depositor

   Angel de Blas

   Article

   George et al J Neurochem. 2021 May;157(4):1032-1051.

   Insert

   ARHGEF9 (Arhgef9 Human, Rat)
   

   Use

   RNAi

   Promoter

   mouse U6 promoter

   Available Since

   Oct. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. CBSH3- shRNA3

    Plasmid

    #160212

    Purpose

    Knock Down MYDSH3- Collybistin isoforms

    Depositor

    Angel de Blas

    Article

    George et al J Neurochem. 2021 May;157(4):1032-1051.

    Insert

    ARHGEF9 (Arhgef9 Human, Rat)
    

    Use

    RNAi

    Promoter

    mouse U6 promoter

    Available Since

    Oct. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. CBSH3- 3m shRNA2

    Plasmid

    #160211

    Purpose

    Knock Down MTLSH3- Collybistin isoforms, 3-point mutation negative control for CBSH3- shRNA2 targeting collybistin MTL-CBSH3-isoforms (Plasmid #160210)

    Depositor

    Angel de Blas

    Article

    George et al J Neurochem. 2021 May;157(4):1032-1051.

    Insert

    ARHGEF9 (Arhgef9 Rat)
    

    Use

    RNAi

    Mutation

    TGACGTTGCTCaGGcTGtACCA (mutations shown in lower …

    Promoter

    mouse U6 promoter

    Available Since

    Oct. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. CBSH3- 3m shRNA1

    Plasmid

    #160209

    Purpose

    Knock Down MQWSH3- Collybistin isoforms, 3-point mutation negative control for CBSH3- shRNA1 targeting collybistin MQW-CBSH3- isoforms (Plasmid # 160208).

    Depositor

    Angel de Blas

    Article

    George et al J Neurochem. 2021 May;157(4):1032-1051.

    Insert

    ARHGEF9 (Arhgef9 Rat)
    

    Use

    RNAi

    Mutation

    GATCGGGAATGCTCaGGcTGtACC (mutations shown in lowe…

    Promoter

    mouse U6 promoter

    Available Since

    Oct. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. CBSH3- shRNA1

    Plasmid

    #160208

    Purpose

    Knock Down MQWSH3- Collybistin isoforms

    Depositor

    Angel de Blas

    Article

    George et al J Neurochem. 2021 May;157(4):1032-1051.

    Insert

    ARHGEF9 (Arhgef9 Human, Rat)
    

    Use

    RNAi

    Promoter

    mouse U6 promoter

    Available Since

    Oct. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pAAV-hSyn-DIO-HA-CB2SH3(-)-IRES-mCitrine

    Plasmid

    #160069

    Purpose

    Conditionally overexpress collybistin (CB2SH3-) and mCitrine

    Depositor

    Angel de Blas

    Article

    George et al eNeuro. 2021 Jun 2. pii: ENEURO.0561-20.

    Insert

    ARHGEF9 (Arhgef9 Rat)
    

    Use

    AAV

    Tags

    HA- in CB2SH3

    Promoter

    human Synapsin promoter

    Available Since

    Oct. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. S2 1058 APOBEC3 no UGI

    Plasmid

    #135350

    Purpose

    Expression of intradomain insertion of rat cytosine deaminase, rAPOBEC3, at position 1058 of nSpyCas9 lacking uracil DNA glycosylase inhibitor.

    Depositor

    Alex Hewitt

    Article

    Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

    Insert

    nSpyCas9-rAPOBEC3-ID-1058
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    WT

    Available Since

    Oct. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. S1 1029 CP APOBEC3 no UGI

    Plasmid

    #135352

    Purpose

    Expression of circular permutant of nSpyCas9 lacking uracil DNA glycosylase inhibitor at amino acid position 1029 encoding rat cytosine deaminase, rAPOBEC3 at the N-terminus

    Depositor

    Alex Hewitt

    Article

    Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

    Insert

    rAPOBEC3-nSpyCas9 aa 1029
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    WT

    Available Since

    Oct. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. S1 1029 AIDx with UGI

    Plasmid

    #135354

    Purpose

    Expression of nSpyCas9 encoding truncated human cytosine deaminase hAIDx at amino acid 1029 and uracil DNA glycosylase inhibitor (UGI) at the C terminus

    Depositor

    Alex Hewitt

    Article

    Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

    Insert

    hAIDx-nSpyCas9 aa-UGI
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    WT

    Available Since

    Oct. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pcDNA-HisFlag-rUSP19ER-CRmut

    Plasmid

    #155253

    Purpose

    for mammalian overexpression of the mutated ER isoform of rat USP19

    Depositor

    Simon Wing

    Insert

    USP19 (ER isoform) CR mutant (Usp19 Rat)
    

    Tags

    6XHis-3XFlag

    Expression

    Mammalian

    Mutation

    Cys(545) to Arg

    Promoter

    CMV

    Available Since

    Sept. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pEGFP-rUSP19CYT-CAmut

    Plasmid

    #155250

    Purpose

    for mammalian overexpression of the mutated CYT isoform of rat USP19 fused to GFP at N-terminus

    Depositor

    Simon Wing

    Insert

    USP19 (Cytoplasmic isoform) CA mutant (Usp19 Rat)
    

    Expression

    Mammalian

    Mutation

    Cys(545) to Ala

    Promoter

    CMV

    Available Since

    Sept. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pEGFP-rUSP19ER-CAmut

    Plasmid

    #155249

    Purpose

    for mammalian overexpression of the mutated ER isoform of rat USP19 fused to GFP at N-terminus

    Depositor

    Simon Wing

    Insert

    USP19 (ER isoform) CA mutant (Usp19 Rat)
    

    Expression

    Mammalian

    Mutation

    Cys(545) to Ala

    Promoter

    CMV

    Available Since

    Sept. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge