We narrowed to 2,718 results for: fas

1. pAAV-EF1a-Dio-ASAP5-Kv2.1-WPRE

   Plasmid

   #225708

   Purpose

   Cre dependent soma-targeted expression of the genetically encoded voltage indicator ASAP5

   Depositor

   Michael Lin

   Article

   Hao et al Neuron. 2024 Sep 17:S0896-6273(24)00643-

   Has Service

   AAV9

   Insert

   ASAP5-Kv2.1
   

   Use

   AAV

   Promoter

   EF1α

   Available Since

   Oct. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. evoFERNY-Cas9NG

   Plasmid

   #203329

   Purpose

   Plasmid for bacterial purification of codon optimized evoFERNY-Cas9NG

   Depositor

   Vijay Sankaran

   Article

   Martin-Rufino et al Cell. 2023 Apr 27:S0092-8674(23)00332-X.

   Insert

   evoFERNY-Cas9NG
   

   Use

   CRISPR

   Tags

   His-tag

   Expression

   Bacterial

   Available Since

   June 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLSB-KAN

   Plasmid

   #166700

   Purpose

   Combined sgRNA/Cas9 pLSB vector with kanMX6 (G418) marker. Golden Gate-ready for cloning custom sgRNA sequences.

   Depositor

   Robin Allshire

   Article

   Torres-Garcia et al Wellcome Open Res. 2020 Nov 24;5:274. do

   Inserts

   tRNA promoter : sgRNA cassette
   adh15 promoter : Cas9 codon-optimised for S. pombe
   

   Expression

   Yeast

   Promoter

   adh15 promoter and tRNA Ser promoter

   Available Since

   April 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. ABE8e-Cas9NG

   Plasmid

   #203328

   Purpose

   Plasmid for bacterial purification of codon optimized ABE8e-Cas9NG

   Depositor

   Vijay Sankaran

   Article

   Martin-Rufino et al Cell. 2023 Apr 27:S0092-8674(23)00332-X.

   Insert

   ABE8e-Cas9NG
   

   Use

   CRISPR

   Tags

   His-tag

   Expression

   Bacterial

   Available Since

   Oct. 17, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLSB-HYG

   Plasmid

   #166699

   Purpose

   Combined sgRNA/Cas9 pLSB vector with hphMX6 (hygromycin) marker. Golden Gate-ready for cloning custom sgRNA sequences.

   Depositor

   Robin Allshire

   Article

   Torres-Garcia et al Wellcome Open Res. 2020 Nov 24;5:274. do

   Inserts

   tRNA promoter : sgRNA cassette
   adh15 promoter : Cas9 codon-optimised for S. pombe
   

   Expression

   Yeast

   Promoter

   adh15 promoter and tRNA promoter

   Available Since

   April 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. FUW-TetON-GFP

   Plasmid

   #115495

   Purpose

   Lentiviral plasmid for tet-inducible expression of GFP (generated from plasmid #20321)

   Depositor

   Catherine Verfaillie

   Article

   Garcia-Leon et al Stem Cell Reports. 2018 Feb 13;10(2):655

   Insert

   eGFP
   

   Use

   Lentiviral

   Available Since

   Nov. 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. AAVS1-TRE3G-NFIA+SOX9

   Plasmid

   #129455

   Purpose

   Inducible overexpression of NFIA and Sox9 in human cells.

   Depositor

   Su-Chun Zhang

   Article

   Li et al Stem Cell Reports. 2018 Oct 9;11(4):998-

   Insert

   Sox 9 (SOX9 Human)
   

   Expression

   Mammalian

   Promoter

   TRE3G

   Available Since

   March 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pRibo-Sec-APEX2m

   Plasmid

   #176844

   Purpose

   Expression of APEX2 in the periplasm from a codon-optimized gene (multi-copy episomal plasmid)

   Depositor

   Jessica Seeliger

   Article

   Ahamed et al Methods Enzymol. 2022;664:267-289. doi:

   Insert

   pRibo-Sec-APEX2m
   

   Use

   Inducible

   Expression

   Bacterial

   Promoter

   pRibo, theophilline riboswitch inducible hsp60 pr…

   Available Since

   Jan. 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-EF1a-DIO-mGreenLantern

   Plasmid

   #164468

   Purpose

   Cre-dependent EF1a-driven expression of mGreenLantern in animals using AAV

   Depositor

   Gregory Petsko

   Article

   Campbell et al Proc Natl Acad Sci U S A. 2020 Dec 1;117

   Insert

   mGreenLantern
   

   Use

   AAV

   Expression

   Mammalian

   Mutation

   Clover-F64L/S72A/E124V/N149K/I167T/S175G/A206K/L2…

   Promoter

   EF1a

   Available Since

   Jan. 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. His-ZZ-TEV-SNAPf-BICDR1

    Plasmid

    #111858

    Purpose

    N-terminal SNAPf tag on BICDR1 for expression in Sf9 cells. 8xHis-ZZ-SNAPf N-terminal tag, TEV site to cleave 8xHis-ZZ.

    Depositor

    Andrew Carter

    Article

    Urnavicius et al Nature. 2018 Feb 7;554(7691):202-206. do

    Insert

    BICDR1 (Bicdl1 Mouse)
    

    Tags

    His-tag, SNAPf-tag, TEV site, and ZZ

    Expression

    Insect

    Mutation

    R250Q, Optimised for Sf9 expression

    Available Since

    July 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pEGFPN1-N-gamma-tubulin (334-449)

    Plasmid

    #87859

    Purpose

    Fluorescent fragment of gamma-tubulin containing gamma-tubulin DNA binding domain and NLS

    Depositor

    Maria Alvarado-Kristensson

    Article

    Hoog et al FASEB J. 2011 Nov;25(11):3815-27. doi: 1

    Insert

    gamma-tubulin 1 (TUBG1 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    fragment from aa 334 to 449

    Available Since

    May 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLJM1-PHD2

    Plasmid

    #223552

    Purpose

    Lentivirus transfer plasmid for expression of full length human PHD2

    Depositor

    Somshuvra Mukhopadhyay

    Article

    Gurol et al Proc Natl Acad Sci U S A. 2024 Jun 25;12

    Insert

    PHD2 (EGLN1 Human)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. AP1muA single guide RNA

    Plasmid

    #230030

    Purpose

    guide RNA for AP1muA tagging at the C-terminus in pX459

    Depositor

    Francesca Bottanelli

    Article

    Stockhammer et al J Cell Sci. 2024 Aug 15;137(16):jcs26195

    Insert

    AP1M1 guide (AP1M1 Human)
    

    Use

    CRISPR

    Available Since

    Jan. 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. His-ZZ-TEV-BICDR1-GFP

    Plasmid

    #111657

    Purpose

    C-terminal GFP tag on BICDR1 for expression in Sf9 cells. 8xHis-ZZ-GFP N-terminal tag, TEV site to cleave 8xHis-ZZ.

    Depositor

    Andrew Carter

    Article

    Urnavicius et al Nature. 2018 Feb 7;554(7691):202-206. do

    Insert

    BICDR1-GFP (Bicdl1 Mouse)
    

    Tags

    8xHis-tag, GFP, TEV site, and ZZ

    Expression

    Insect

    Mutation

    Optimised for Sf9 expression

    Available Since

    July 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. px330-CIB1-dCas9

    Plasmid

    #63665

    Purpose

    Mammalian gRNA expression vector also expressing CIB1-dCas9

    Depositor

    Dong Kong

    Article

    Chen et al Theranostics. 2018 Aug 7;8(16):4447-4461

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6 and CMV

    Available Since

    Sept. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. RAT

    Plasmid

    #187416

    Purpose

    Expresses Na,K-ATPase (ATP1A1 and ATP1B1 in pFastBac Dual vector) in insect cells

    Depositor

    Susanne Dobler

    Article

    Mohammadi et al bioRxiv 2021.11.29.470343

    Inserts

    ATP1A1 (Atp1a1 Rat)
    ATP1B1 (Atp1b1 Rat)
    

    Expression

    Insect

    Promoter

    PH and p10

    Available Since

    Oct. 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCytERM-mGreenLantern

    Plasmid

    #164467

    Purpose

    Visualization of the ER in cells (compatible with OSER assay)

    Depositor

    Gregory Petsko

    Article

    Campbell et al Proc Natl Acad Sci U S A. 2020 Dec 1;117

    Insert

    mGreenLantern
    

    Tags

    Cytochrome p450 first 29 animo acids (Oryctolagus…

    Expression

    Mammalian

    Mutation

    Clover-F64L/S72A/E124V/N149K/I167T/S175G/A206K/L2…

    Promoter

    CMV

    Available Since

    Jan. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3.1 E2F1 HA(Δ2-126) (HindIII- BamHI- EcoRI)

    Plasmid

    #70668

    Purpose

    Human mutant of E2F1 lacking amino acids 2 to 126

    Depositor

    Maria Alvarado-Kristensson

    Article

    Hoog et al FASEB J. 2011 Nov;25(11):3815-27. doi: 1

    Insert

    E2F1 lacking residues 2 to 126 (E2F1 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    lacks amino acids 2-126

    Promoter

    cmv

    Available Since

    Nov. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

19. pLPCX2-HA-GATA3 C1(259-443)

    Plasmid

    #89832

    Purpose

    Express GATA3 C1 in mammalian cells

    Depositor

    Shengyu Yang

    Article

    Sun et al J Biol Chem. 2013 Dec 27;288(52):36971-8

    Insert

    Gata3 (Gata3 Mouse)
    

    Use

    Retroviral

    Tags

    HA

    Mutation

    C1 (AA 259-443)

    Promoter

    CMV

    Available Since

    May 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. His-ZZ-TEV-BICDN(1-400)-GFP

    Plasmid

    #111862

    Purpose

    C-terminal GFP tag on BICDN (amino acids 1-400) for baculovirus expression in Sf9 cells. 8xHis-ZZ N-terminal tag, TEV site to cleave 8xHis-ZZ.

    Depositor

    Andrew Carter

    Article

    Urnavicius et al Nature. 2018 Feb 7;554(7691):202-206. do

    Insert

    BICDN(1-400)-GFP (Bicd2 Mouse)
    

    Tags

    8xHis tag, GFP, and ZZ tag

    Expression

    Insect

    Mutation

    Codon optimized for Sf9 expression

    Available Since

    July 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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